LCGC Europe最新文献_第4页

LCGC Europe Pub Date : 2022-10-01 DOI: 10.56530/lcgc.eu.dk3677x5

R. Mcdowall

引用次数: 0

A Hydrophilic Strong Anion-Exchange Hybrid Monolith for Capillary Liquid Chromatography 一种用于毛细管液相色谱的亲水性强阴离子交换杂化单体

LCGC Europe Pub Date : 2022-09-01 DOI: 10.56530/lcgc.eu.od3570x2

Jinxiang Li, Jun Liu, Qianfeng Weng

引用次数: 0

Application of Liquid- and Supercritical Fluid Chromatography Coupled with High-Resolution Mass Spectrometry for the Analysis of Short-, Medium-, and Long-Chain Chlorinated Paraffins in Dietary Supplements 液相和超临界流体色谱-高分辨率质谱联用分析膳食补充剂中短链、中链和长链氯化石蜡的应用

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.qd8477p7

Jakub Tomasko, David Maxa, K. Navratilova, Tomas Kourimsky, V. Hrbek, J. Hajšlová, J. Pulkrabová

引用次数: 0

Quo Vadis Analytical Procedure Development and Validation? 分析程序的开发和验证?

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.ue1079n4

Chris Burgess

引用次数: 1

Dispersive Solid-Phase Extraction and Solid-Phase Extraction for ppt-level PFAS Analysis in Apples: A Comparison 分散固相萃取法与固相萃取法分析苹果中ppt水平PFAS的比较

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.tp4376p2

Marise van der Vegt, Ruben Kause, B. Berendsen, Stefan van Leeuwen

引用次数: 0

Nonlinear Predictive Modelling Enables In Silico Optimization of Chromatographic Methods for Complex Stationary Phase‑Analyte Interactions 非线性预测建模使复杂固定相分析物相互作用的色谱方法在硅优化

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.uf5786p6

I. H. Ahmad, G. L. Losacco, E. Regalado

{"title":"Nonlinear Predictive Modelling Enables In Silico Optimization of Chromatographic Methods for Complex Stationary Phase‑Analyte Interactions","authors":"I. H. Ahmad, G. L. Losacco, E. Regalado","doi":"10.56530/lcgc.eu.uf5786p6","DOIUrl":"https://doi.org/10.56530/lcgc.eu.uf5786p6","url":null,"abstract":"The development of robust analytical assays for separation and analysis of complex multicomponent mixtures can often be challenging, reflecting the increased complexity of new medicine and vaccine processes. In silico liquid chromatography (LC) method development strategies for small molecules have reached a mature stage across the pharmaceutical industry. However, a straightforward approach for large molecules remains elusive because of conformational changes that can often influence chromatographic retention. Nonetheless, an excellent correlation between experimental and predicted retention time is possible by deploying the correct regression retention models in terms of ln k vs. %B and ln k vs. 1/T (ΔtR < 0.1%). Excellent outcomes generated through in silico chromatographic method development of large molecules using different chaotropic and denaturing mobile phases are illustrated. Linear and nonlinear (polynomial regression) retention models using readily available software were deployed as a function of several chromatographic parameters (gradient slope and column temperature) for a variety of proteins (12–670 kDa) and peptides.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116915915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of MCPD and Glycidyl Fatty Acid Esters in Refined Plant Oils by Supercritical Fluid Chromatography– High-Resolution Mass Spectrometry

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.bl7965l5

Tomas Kourimsky, V. Hrbek, Martin Steidl, J. Hajšlová

{"title":"Analysis of MCPD and Glycidyl Fatty Acid Esters in Refined Plant Oils by Supercritical Fluid Chromatography– High-Resolution Mass Spectrometry","authors":"Tomas Kourimsky, V. Hrbek, Martin Steidl, J. Hajšlová","doi":"10.56530/lcgc.eu.bl7965l5","DOIUrl":"https://doi.org/10.56530/lcgc.eu.bl7965l5","url":null,"abstract":"2- and 3-chloropropane-1,2-diol (2- and 3-MCPD) and their fatty acid esters (2- and 3-MCPDEs) together with glycidyl fatty acids esters (GEs) belong to a group of process-induced contaminants. They are formed in high temperature environments and most commonly occur in refined vegetable oils. Since 2021, maximum limits for GEs and 3-MCPDEs have been enforceable in the EU, and the requirement for adequate analytical methods is constantly increasing. The aim of this study was to develop an efficient method to enable a rapid determination of nine 3-MCPDEs and seven GEs in vegetable oils within a single run employing supercritical fluid chromatography coupled to high-resolution tandem mass spectrometry (SFC–HRMS/MS). Contrary to a routine gas chromatography mass spectrometry method aimed at determination of the total MCPD pool, the sample throughput when using SFC–HRMS/MS was greatly increased, as the “dilute-and shoot” approach did not require any hydrolysis and derivatization. Additionally, the pattern of natural MCPDEs was characterized. The performance characteristics of this new method met the criteria required by Commision Regulation 2019/2093 for all target analytes.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127960686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Authentication of Panax Ginseng‑Based Herbal Teas Using “Chemical Markers” Strategy 用“化学标记”方法鉴定人参草药茶

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.ig4675a1

Jana Kvirencova, V. Hrbek, M. Tomaniová, J. Hajšlová

引用次数: 0

Analysis of UV-Treated Mushrooms: Dietary Source of Vitamin D2? 紫外线处理蘑菇的分析:维生素D2的膳食来源?

LCGC Europe Pub Date : 2022-07-01 DOI: 10.56530/lcgc.eu.wi6189z4

L. Drábová, Lenka Libenská, Markéta Zedníková, Veronika Vondrášková, J. Hajšlová, J. Pulkrabová

{"title":"Analysis of UV-Treated Mushrooms: Dietary Source of Vitamin D2?","authors":"L. Drábová, Lenka Libenská, Markéta Zedníková, Veronika Vondrášková, J. Hajšlová, J. Pulkrabová","doi":"10.56530/lcgc.eu.wi6189z4","DOIUrl":"https://doi.org/10.56530/lcgc.eu.wi6189z4","url":null,"abstract":"In recent years, dietary intake of vitamin D has become an issue of high concern because this bioactive molecule boosts the immune system and is presumed to provide some protection against Covid-19. Under these conditions, a search for nontraditional dietary sources has appeared as a new challenge. One of the possibilities is irradiation of champignons that contain a high amount of ergosterol, a vitamin D2 precursor. In our study, a fast and sensitive liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method for the determination of vitamin D2 in fresh mushrooms and its metabolite 25(OH)D2 in the blood of volunteers regularly consuming UV-treated mushrooms has been introduced. For extraction of desiccated mushrooms, solid-liquid extraction n-hexane–ethyl acetate was used, and n-hexane was employed for blood plasma samples. Separation of target analytes was performed on a polymeric bonding C18 phase column. Satisfactory limits of quantification (LOQs) were reached both for the control of vitamin D2 content in mushrooms (LOQ = 10 ng/g) and for the monitoring of vitamin D2 and D3 metabolite in human blood (LOQ = 2.5 ng/mL). For accurate quantification, isotopic dilution was employed.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124210250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide Profiling 利用液相色谱-质谱法深入研究体外转录mRNA的结构细节

LCGC Europe Pub Date : 2022-06-01 DOI: 10.56530/lcgc.eu.jk3969w4

Kris Morreel, R. T'kindt, G. Debyser, Stefanie Jonckheere, P. Sandra

{"title":"Diving into the Structural Details of In Vitro Transcribed mRNA Using Liquid Chromatography–Mass Spectrometry-Based Oligonucleotide Profiling","authors":"Kris Morreel, R. T'kindt, G. Debyser, Stefanie Jonckheere, P. Sandra","doi":"10.56530/lcgc.eu.jk3969w4","DOIUrl":"https://doi.org/10.56530/lcgc.eu.jk3969w4","url":null,"abstract":"The production process of in vitro transcribed messenger RNA (IVT-mRNA)-based vaccines has matured in recent years, partly due to the fight against infectious diseases such as COVID-19. One key to success has been the use of modified, next to canonical, nucleotides and the efficient addition of a Cap-structure and poly A tail to the 5’ and 3’ end, respectively, of this massive biomolecule. These important features affect mRNA stability and impact translation efficiency, consequently boosting the optimization and implementation of liquid chromatography–mass spectrometry (LC–MS)-based oligonucleotide profiling methods for their characterization. This article will provide an overview of these LC–MS methods at a fundamental and application level. It will be shown how LC–MS is implemented in mRNA-based vaccine analysis to determine the capping efficiency and the poly A tail length, and how it allows, via RNA mapping, (i) to determine the mRNA sequence, (ii) to screen the fidelity of the manufactured modifications, and (iii) to identify and quantify unwanted modifications resulting from manufacturing or storage, and sequence variants resulting from mutation or transcription errors.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124795610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1