Open Biomarkers Journal最新文献_第8页

Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions 分离尿中游离DNA和细胞DNA的方法——甲基化特异性PCR在两种DNA片段上的应用

Open Biomarkers Journal Pub Date : 2011-12-02 DOI: 10.2174/1875318301104010015

Agnes Beermann, Foued Ghanjati, T. Hermanns, C. Poyet, J. Pereira, J. Fischer, P. Wernet, S. Santourlidis

引用次数: 4

”BIK/NBK Gene Expression as a Possible Marker of Circulating BreastCancer Cells in Blood“ 《BIK/NBK基因表达作为血液循环乳腺癌细胞的可能标记》

Open Biomarkers Journal Pub Date : 2011-10-14 DOI: 10.2174/1875318301104010008

E. López-Muñoz, N. García-Hernández, Rosenda I. Peñaloza-Espinosa, M. Toro, Gelasio Zarco-Espinosa, S. Barroso-Bravo, F. Salamanca-Gómez, D. Arenas-Aranda

{"title":"”BIK/NBK Gene Expression as a Possible Marker of Circulating BreastCancer Cells in Blood“","authors":"E. López-Muñoz, N. García-Hernández, Rosenda I. Peñaloza-Espinosa, M. Toro, Gelasio Zarco-Espinosa, S. Barroso-Bravo, F. Salamanca-Gómez, D. Arenas-Aranda","doi":"10.2174/1875318301104010008","DOIUrl":"https://doi.org/10.2174/1875318301104010008","url":null,"abstract":"The detection of circulating breast cancer cells in blood could be of special interest as an indicator of diagnosis and prognosis, and for the selection of treatment. In a previous report, our research group determined gene expression pro- files in samples of breast cancer tissue, identifying over-expression of the BIK/NBK mRNA gene in 90% of the analyzed samples. In this paper, we analyze the BIK/NBK gene expression as a possible biomarker of circulating breast cancer cells in blood. We demonstrate that the BIK/NBK gene expression is not a significant biomarker in the detection of circulating breast cancer cells in the blood of women with breast cancer. Several studies have evaluated the regulation of apoptosis by estrogens in breast cancer cells, demonstrating the importance of BIK/NBK protein, in estrogen-regulated breast cancer cell apoptosis, which suggests that the regulation of its expression may be an important therapeutic target or strategy in the management of cancer, and, although we did not find statistically significant differences among the patient groups to demonstrate that BIK/NBK gene expression is a biomarker of circulating breast cancer cells in blood, we consider it neces- sary to continue the study of this gene in breast cancer tissue and its role in the development and progression of breast cancer, its prognostic value, and its potential use as therapeutic target.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"4 1","pages":"8-14"},"PeriodicalIF":0.0,"publicationDate":"2011-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Relationship Between Cigarette Smoking and Biomarkers of Exposure Across Two Study Centers in Europe 欧洲两个研究中心吸烟与暴露生物标志物之间的关系

Open Biomarkers Journal Pub Date : 2010-07-08 DOI: 10.2174/1875318301003010021

F. Lowe, E. Gregg, A. Bassi, R. Puntoni

{"title":"The Relationship Between Cigarette Smoking and Biomarkers of Exposure Across Two Study Centers in Europe","authors":"F. Lowe, E. Gregg, A. Bassi, R. Puntoni","doi":"10.2174/1875318301003010021","DOIUrl":"https://doi.org/10.2174/1875318301003010021","url":null,"abstract":"The relationship between biomarkers of exposure to cigarette smoke in 24h urine samples collected from groups of 80 smokers (44 males, 36 females) and 40 never smokers (17 males, 23 females) at two centers in Europe was studied. Eight biomarkers (nicotine, cotinine, hydroxycotinine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and all of the respective glucuronide conjugates) were measured. Subjects from the two centers were pooled and bio- marker data analyzed according to the machine smoked tar yield of the brand each subject smoked and the recorded num- ber of cigarettes smoked per day (CPD). A statistically significant relationship between CPD and all of the biomarkers analyzed was found. Smokers of less than 11 CPD had the lowest mean 24h urinary concentrations for all biomarkers measured. However, if the amount of constituent obtained from each cigarette smoked was calculated, then the amount of nicotine obtained per cigarette was highest in this group although the variation was also greatest for this group. The amount of NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1 butanone, the parent molecule of NNAL) obtained per cigarette was not statistically significantly different across all groups. In conclusion, these results confirm the reliability of 24h uri- nary total nicotine and NNAL concentrations as biomarkers of exposure to specific cigarette smoke constituents across two centers in Europe. These measurements may provide an objective alternative to CPD when grouping smokers for are studies of other endpoints.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"16 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2010-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68111157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Autoantibodies in Breast Cancer Identify Proteins Involved in Self- Renewal and Epigenetic Chromatin Remodeling 乳腺癌自身抗体鉴定参与自我更新和表观遗传染色质重塑的蛋白

Open Biomarkers Journal Pub Date : 2010-03-25 DOI: 10.2174/1875318301003010013

F. Madrid, Wei Chen, N. Tang, Xinbo Zhang, Jinsheng Dong, H. Sagar, J. Abrams

{"title":"Autoantibodies in Breast Cancer Identify Proteins Involved in Self- Renewal and Epigenetic Chromatin Remodeling","authors":"F. Madrid, Wei Chen, N. Tang, Xinbo Zhang, Jinsheng Dong, H. Sagar, J. Abrams","doi":"10.2174/1875318301003010013","DOIUrl":"https://doi.org/10.2174/1875318301003010013","url":null,"abstract":"Recent studies have shown that autoantibodies developing in cancer patients are tumor-associated and are promising biomarkers for the diagnosis and prognosis of cancer. Here we report a panel of signal transduction molecules with partial sequences identical to the oncogene Bmi-1, NIFK, a nucleolar protein interacting with the FHA domain of Ki- 67, TAB182, a protein interacting with tankyrase-1, CNOT6L, a subunit of the CCR4-NOT complex and to Elp3, a subunit of the elongator complex that are recognized as autoantigens by breast cancer sera with the ability to discriminate between invasive breast cancer and normal sera with high sensitivity and specificity. The proteins bearing the epitopes recognized by these antibodies have conserved regions involved in protein-protein interactions participating in regulatory processes such as self renewal, cell proliferation and survival, chromatin modulation, transcriptional silencing and organ patterning, usually ascribed to stem cell function. In this work we demonstrate by autoantigen microarray analysis that autoantibodies in breast cancer sera have the potential to delineate pathway connectivity. Our data indicate that several pathways involved in maintaining telomere stability are the target of an autoimmune reaction in breast cancer. These find- ings suggest that autoantibodies with the ability to recognize autoantigens involved in self-renewal and epigenetic chro- matin remodeling have the potential to predict an invasive tendency of breast cancer.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"162 1","pages":"13-20"},"PeriodicalIF":0.0,"publicationDate":"2010-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68111147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Identification of Serum Biomarkers in End Stage Liver Disease 终末期肝病血清生物标志物的鉴定

Open Biomarkers Journal Pub Date : 2010-01-29 DOI: 10.2174/1875318301003010001

D. Koutsogiannis, K. Summers, Biju George, P. Adams, P. Marotta, S. Chakrabarti

{"title":"Identification of Serum Biomarkers in End Stage Liver Disease","authors":"D. Koutsogiannis, K. Summers, Biju George, P. Adams, P. Marotta, S. Chakrabarti","doi":"10.2174/1875318301003010001","DOIUrl":"https://doi.org/10.2174/1875318301003010001","url":null,"abstract":"Background: Progressive fibrosis and cirrhosis, clinically presenting as end-stage liver disease are common outcomes in alcoholic hepatitis as well as non-alcoholic fatty liver disease(NAFLD). In these processes, a series of changes occurs in liver tissues leading to cell death, remodeling, fibrosis and regeneration. The aim of this study is to identify potential novel biomarkers for non-invasive diagnosis of cirrhosis due to alcoholic etiology or NAFLD. Methods: Serum from patients with biopsy proven end-stage liver disease of various etiologies, namely NAFLD(n=9), al- cohol(n=5), and other end-stage liver diseases(n=6), who underwent liver transplant during the first six months of 2007 were utilized for retrospective analysis. Serum samples were also collected from a group of healthy volunteers (n=7). The samples were analysed using Luminex technology or ELISA for 27 biomarkers that are known to be involved in patho- logic processes such as cell death, regeneration and fibrosis. Results: Of the 27 serum markers examined, 16 were elevated in the serum in all groups with end-stage liver diseases compared with the control group. They include adipokines, apoptosis and inflammatory mediators and growth factors. In- terestingly, the serum of NAFLD patients showed significantly elevated HGF levels and trend towards increase in sFAS, TGF￻ 1, TNFR-1, TNFR-2 and leptin. The level of serum markers showed excellent correlation with each other indicating a complex interdependent pathogenetic mechanism. Conclusions: The data from this study indicate that a large number of serum markers are altered in end-stage liver dis- eases. A panel of such markers may potentially be useful in assessing advanced fibrosis and cirrhosis in patients with chronic end stage liver diseases.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"3 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2010-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Sigma-2 Receptors as Potential Novel Biomarkers During the Progression of Benign Prostatic Hypertrophy (BPH) into Prostate Cancer 良性前列腺肥大(BPH)向前列腺癌发展过程中Sigma-2受体作为潜在的新型生物标志物

Open Biomarkers Journal Pub Date : 2009-06-19 DOI: 10.2174/1875318300902010011

N. Colabufo, P. Saponaro, M. Bottalico, M. Contino, C. Inglese, V. Pagliarulo, A. Pagliarulo, F. Berardi, R. Perrone

引用次数: 5

Quantitative Analysis of the Expression of Human N-myristoyltransferase 1 (hNMT-1) in Cancers 人n -肉豆芽酰基转移酶1 (hNMT-1)在肿瘤组织中的表达

Open Biomarkers Journal Pub Date : 2009-03-13 DOI: 10.2174/1875318300902010006

Lifeng Chen, Binbing Ling, J. Alcorn, Jian Yang

引用次数: 9

The Effect of n-6 Polyunsaturated Fatty Acid on Blood Levels of Malondialdehyde-Deoxyguanosine Adducts in Human Subjects~!2008-07-08~!2008-11-06~!2008-12-05~! n-6多不饱和脂肪酸对人体血液丙二醛-脱氧鸟苷加合物水平的影响

Open Biomarkers Journal Pub Date : 2008-12-05 DOI: 10.2174/1875318300801010028

S. A. Moore, E. Humphreys, Friesen, D. Shuker, S. Bingham

引用次数: 3

Aberrant Cytoplasmic Accumulation of Connexin 43 in Human Testicular Seminoma~!2008-07-21~!2008-10-15~!2008-12-05~! 人睾丸精原细胞瘤细胞质中连接蛋白43的异常积累

Open Biomarkers Journal Pub Date : 2008-12-05 DOI: 10.2174/1875318300801010020

V. Mauro, D. Chevallier, J. Gilleron, N. Defamie, D. Carette, J. Gasc, F. Sénégas-Balas, D. Segretain, G. Pointis

{"title":"Aberrant Cytoplasmic Accumulation of Connexin 43 in Human Testicular Seminoma~!2008-07-21~!2008-10-15~!2008-12-05~!","authors":"V. Mauro, D. Chevallier, J. Gilleron, N. Defamie, D. Carette, J. Gasc, F. Sénégas-Balas, D. Segretain, G. Pointis","doi":"10.2174/1875318300801010020","DOIUrl":"https://doi.org/10.2174/1875318300801010020","url":null,"abstract":"In the present study Cx43 mRNA and protein were analyzed in germ cells of men with normal spermatogenesis and in human testicular seminoma. In normal testis Cx43 mRNAs were basally located within seminiferous tubules and expressed in the most basally located germ cells (spermatogonia, early spermatocytes, and pachytene spermatocytes) and in Sertoli cells. Immunofluorescence analysis showed that Cx43 signal was mainly located in the basal compartment of seminiferous tubules and was stage-dependent. Cx43 mRNAs were also detected in human testicular seminoma. Transcripts were present within seminoma cells identified by PLAP staining. However, Cx43 protein exhibited an intracytoplasmic accumulation, within an intracellular compartment distinct from the Golgi apparatus and was undetectable at the plasma membrane level, suggesting post-translational rather than transcriptional abnormalities. This aberrant intracytoplasmic accumulation of Cx43 is due neither to a dysfunction of the protein trafficking machinery nor to a specific alteration of its major protein partner, ZO-1, since the tight junction associated protein was detected at the plasma membrane level and did not colocalize with Cx43.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"1 1","pages":"20-27"},"PeriodicalIF":0.0,"publicationDate":"2008-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68111139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Novel Risk Factors for Atherosclerosis~!2008-08-22~!2008-11-09~!2008-12-05~! 动脉粥样硬化的新危险因素

Open Biomarkers Journal Pub Date : 2008-12-05 DOI: 10.2174/1875318300801010036

A. Papazafiropoulou, N. Katsilambros, N. Tentolouris

引用次数: 4