Open Biomarkers Journal最新文献

{"title":"The Cytoglobin Expression Under Hypoxic Conditions in Covid-19 Cases","authors":"Endah Wulandari, R. Ayu, Fitri Hapsari, Francisca A Tjakradidjaja, Auliyani Andam Suri","doi":"10.2174/18753183-v13-230427-2023-2","DOIUrl":"https://doi.org/10.2174/18753183-v13-230427-2023-2","url":null,"abstract":"\u0000 \u0000 Cytoglobin (Cygb) is an oxygen transporter marker that appears in hypoxic conditions. The clinical condition of Corona Virus Disease 2019 (COVID-19) cases, in general is that patients experience hypoxemia with low oxygen saturation. The Cygb gene is stimulated by the Hypoxia-Inducible Factor-1alpha (HIF-1α) transcription factor, which is stable in hypoxia.\u0000 \u0000 \u0000 \u0000 This study investigates Cygb expression in hypoxic COVID-19 cases. The design of this research is analytically observational. Parameters measured were Cygb mRNA and protein levels, correlation of HIF-1α and Cygb proteins in COVID-19 patients with Alpha, Beta, Delta, Omicron variants and negative control patients.\u0000 \u0000 \u0000 \u0000 The results showed that each Cygb mRNA level decreased by 0.50, 0.92, 0.75 and 0.84 times that of the control. In contrast, Cygb protein levels (ng/mL) increased (16.95; 20.33; 21.20; 14.01 and 6.29 control). Strong negative correlation between mRNA and Cygb protein (R = -0.611). Strong positive correlation between HIF-1α and Cygb protein (R = 0.670).\u0000 \u0000 \u0000 \u0000 This study showed that Cygb mRNA expression decreased, further increasing Cygb protein; HIF-1α protein levels increased, further increasing Cygb protein. In COVID-19 patients (Alpha, Beta, Delta and Omicron variants), there is an increase in Cygb protein levels through stimulation of HIF-1α, which is stable under hypoxic conditions. The regulation of Cygb in this study has the potential to become the basis for handling cases of viral infections or other cases of hypoxia.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48868558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"microRNA-146a: A biomarker for Epileptogenesis, Epilepsy Prognosis, and Treatment Resistance","authors":"Zachary O. Dent, Liam Chen","doi":"10.2174/18753183-v12-e221226-2022-11","DOIUrl":"https://doi.org/10.2174/18753183-v12-e221226-2022-11","url":null,"abstract":"Recently more attention has been paid to identifying biomarkers for epilepsy to direct a more personalized treatment strategy, especially for patients who suffer from drug-resistant epilepsy which carries a much poorer prognosis. microRNA has emerged as an important and diverse type of biomarker that can participate in metabolic and cellular processes of the disease and, importantly, be detected in patient’s serum. In this short review, we compile state-of-the-art evidence regarding miRNA-146a, a novel biomarker that shows high potential for studying epileptogenesis, monitoring disease progression, evaluating treatment response, and may even functioning as a therapeutic target given its role in the process of neuroinflammation.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47878387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Gender-based Changes in P53 in Preeclampsia","authors":"S. Kharb, S. Gaur, Aparna Khadelwal, Chetna Bhatotiya, S. Nanda","doi":"10.2174/18753183-v12-e2209090","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2209090","url":null,"abstract":"It aims to compare the levels of p53 in maternal and umbilical cord venous samples of healthy pregnant and preeclamptics.\u0000 \u0000 \u0000 Preeclampsia is a leading cause of both maternal morbidity and neonatal mortality. The etiology and pathogenesis of preeclampsia are not yet fully understood. Apoptosis during pregnancy develops due to multiple different mechanisms.\u0000 No studies are available in the literature documenting any association between fetal sex and p53 levels; also, the status of p53 in cord blood is unclear.\u0000 \u0000 \u0000 \u0000 Hence the study was designed to compare p53 levels in maternal and umbilical cord venous samples to study both maternal and fetal aspects of preeclampsia.\u0000 \u0000 \u0000 \u0000 The present study was conducted in 30 normotensive, primigravida women and 30 primigravida preeclamptics (age and gestation matched) with a singleton pregnancy. Serum p53 analysis was carried out in maternal serum and cord blood by solid phase sandwich enzyme-linked immunosorbent assay (Elisa kit).\u0000 \u0000 \u0000 \u0000 In the present study, maternal and cord p53 levels in preeclamptics were higher. The cord blood p53 levels were significantly higher in preeclamptic mothers with female babies than in preeclamptic mothers with male babies.\u0000 \u0000 \u0000 \u0000 These findings indicate a definitive role of apoptosis in the pathogenesis of preeclampsia and may be useful in diagnosing patients with preeclampsia and identifying future natal, perinatal and maternal risks.\u0000 Demonstrating these gender-based changes in p53 levels suggests an active contribution of the placenta in metabolism during pregnancy.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47886170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Mini-review on the Effects of (Carbon) Nanoparticles and Oxidative Stress in Animals","authors":"Rosalin Priyadarshini Jena, Swayam Sriyanka, Rutuparna Dash, B. Paital","doi":"10.2174/18753183-v12-e2209260","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2209260","url":null,"abstract":"\u0000 \u0000 The carbon family nanoparticles are less reviewed for their impact on organisms associated with oxidative stress physiology.\u0000 \u0000 \u0000 \u0000 This review was carried out after collecting literature on the above topic from various sources, including PubMed and Google Scholar.\u0000 \u0000 \u0000 \u0000 The carbon family nanoparticles have tissue-specific impacts on various organisms, which are evident at the molecular level.\u0000 \u0000 \u0000 \u0000 The carbon nanoparticles and molecules of its family need to be very judiciously released as waste to the environment as they may impart toxic effects on organisms.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42458548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy of Red Cell Distribution Width to Platelet Ratio for Detection of Liver Fibrosis Compared with Fibroscan in Chronic Hepatitis B Egyptian patients","authors":"Amira Kamal Gabr, N. Hawash, S. Abd-Elsalam, R. Badawi, Hanan H Soliman","doi":"10.2174/18753183-v12-e2208150","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2208150","url":null,"abstract":"\u0000 \u0000 The decision to treat chronic hepatitis B Virus infection (CHB) may necessitate an assessment of the degree of liver fibrosis. Guidelines recommend Fibroscan examination in such cases. However, it is costly and not widely available. Red cell distribution width (RDW) and platelet count are simple parameters obtained from the blood pictures; and their ratio RDW to platelet ratio (RPR) was claimed to correlate with liver fibrosis. We aimed to assess the ability of RPR to replace the costly fibroscan in the detection of significant fibrosis in chronic hepatitis B patients.\u0000 \u0000 \u0000 \u0000 This cross-sectional study was conducted in the Tropical medicine department, Tanta University, Egypt, between December 2018 and September 2019. One hundred and twenty-five patients with CHB were included and divided according to the fibroscan examination into: Group I: patients with no significant fibrosis (n=66), Group II: patients with significant (≥ F2) fibrosis (n=59). RPR was calculated for all patients and tested against Fibroscan results.\u0000 \u0000 \u0000 \u0000 Both groups were matched in regards to age, sex, viral load, and steatosis. There was a significant positive correlation between the degree of stiffness measured by FibroScan in patients with a significant degree of fibrosis and serum bilirubin, a quantitative polymerase chain reaction of hepatitis B virus DNA (HBV DNA PCR), and fibrosis-4 score (FIB-4 score) (P value= 0.020, 0.049, and 0.0402, respectively). However, RPR was not correlated to the degree of fibrosis in fibroscan examination.\u0000 \u0000 \u0000 \u0000 The accuracy of RDW to platelet ratio (RPR) for the detection of fibrosis in CHB patients is questionable. FIB-4 is correlated with liver stiffness measurement (LSM) in patients with significant fibrosis (F2 or more). Neither RPR, AST to Platelet Ratio Index (APRI) or FIB4 can replace fibroscan for grading of fibrosis in CHB patients for evaluation to start therapy.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46050196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Survivin rs9904341 Polymorphisms and Susceptibility to Acne Vulgaris","authors":"S. El-Beah, E. Elmansoury, M. Alghobary, Manal M. El-Desoky","doi":"10.2174/18753183-v12-e2208050","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2208050","url":null,"abstract":"\u0000 \u0000 Acne vulgaris (AV), common dermatopathology, has a complex etiopathogenesis with a genetic background. The Survivin gene, which encodes an inhibitor of apoptosis protein, has been linked to some dermatologic disorders. The relationship between Survivin gene polymorphisms and AV has not yet been explored.\u0000 \u0000 \u0000 \u0000 To study the effect of survivin gene polymorphism rs9904341 and survivin serum concentration on the development of AV in Egyptian patients.\u0000 \u0000 \u0000 \u0000 Serum survivin was estimated using an enzyme-linked immunosorbent assay. Real-time quantitative PCR using allelic discrimination probes was conducted to investigate the rs9904341 polymorphism in the survivin gene in 118 AV patients and 120 healthy controls.\u0000 \u0000 \u0000 \u0000 The serum survivin levels were significantly higher in AV patients than controls. Also, it was positively correlated with acne severity. The C allele was significantly more observed in acne patients compared to healthy controls. Patients with the C allele had 1.6 times higher odds of exhibiting acne than those with the G allele. The C/C genotype was significantly more observed in cases versus controls. Patients with the C/C genotype had 2.8 times higher odds of exhibiting acne as compared to those with the G/G genotype. However, no significant association was found between genotype distribution and grades of acne severity.\u0000 \u0000 \u0000 \u0000 Results showed significant associations between survivin gene rs9904341 genotypes and AV susceptibility, although it was not related to acne severity and could not be used as a marker of disease activity.\u0000 \u0000 \u0000 \u0000 AV: Acne vulgaris, GAGS: Global Acne Rating System, SNPs: Single nucleotide polymorphisms.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47365753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric and Semantic Analysis of the Global Research on Biomarkers in Personalized Medicine","authors":"Aida Khakimova, F. Rahim, O. Zolotarev","doi":"10.2174/18753183-v12-e220926-2022-3","DOIUrl":"https://doi.org/10.2174/18753183-v12-e220926-2022-3","url":null,"abstract":"\u0000 \u0000 The aims of the research were to study the citation history of popular articles in the field of biomarkers in personalized medicine, to study the use of terms in the sections of articles, and to consider the key terminology of the most-cited articles and its visualization.\u0000 \u0000 \u0000 \u0000 The article describes approaches to the analysis of publication activity in the field of biomarkers and personalized medicine based on the data from the Web of Science.\u0000 \u0000 \u0000 \u0000 The aim of this study is a bibliometric and semantic analysis of the investigation field related to the application of biomarkers for the purposes of personalized medicine.\u0000 \u0000 \u0000 \u0000 The evaluation of a number of publications and its’ citations was carried out. The key terms extracted from the most-cited articles were divided into thematic groups. The number of citations of the most popular articles since 2011 was estimated.\u0000 \u0000 \u0000 \u0000 The citation histories of the top ten articles were considered. Analysis of key terms from different parts of the most-cited articles included statistics and thematic ranking. The comparison of key terms from the most-cited article and the citing articles allowed us to show that the key terminology of the cited article extends to the citing articles. We presented the key terms of the most-cited articles as a terminological map.\u0000 \u0000 \u0000 \u0000 The study of citation of the articles in the field of personalized medicine and biomarkers was based on a survey on the Web of Science. Based on the analysis of a number of citations the trends and citation histories were constructed. The statistical and thematic analysis of the use of keywords in different sections of articles was done. We have shown that the citing articles spread the key terms of the cited article to identify trends in knowledge development which could be presented as a terminological map.\u0000 \u0000 \u0000 \u0000 We presented the results in the form of a terminological map of the latest developments in the field of biomarkers in personalized medicine based on proposed principles.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48947538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AIMS65 and PALBI Scores as Predictors of Six Months’ Mortality in Cirrhotic Patients with Acute Variceal Bleeding","authors":"M. Elhendawy, A. Eldesouky, S. Soliman, Loai Mansour, S. Abd-Elsalam, N. Hawash","doi":"10.2174/18753183-v12-e2207040","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2207040","url":null,"abstract":"\u0000 \u0000 Bleeding gastroesophageal varices are a cause of high mortality among cirrhotic patients. We aimed to investigate late mortality predictors and prognostic models using easily verified factors at admission in cirrhotic patients with acute variceal bleeding (AVB).\u0000 \u0000 \u0000 \u0000 Between January 2020 and June 2020, 142 patients with AVB from Tanta university hospital were included. Investigating multiple prognostic models was done using multiple logistic regression after identifying significant predictors of 6 months' mortality. Mortality prediction accuracy was assessed with area under the receiver operating characteristic (AUROC) curve.\u0000 \u0000 \u0000 \u0000 The 6 months’ overall mortality rate was 31% (44 patients had died). AIMS56, Child-Turcotte-Pugh (CTP) grade C and MELD scores were significantly higher among non survivors (p<0.001) while Platelet-albumin-bilirubin (PALBI) was significantly more negative among survivors (P=0.001). Hepatocellular carcinoma was not significantly related to the mortality (p =0.364). Univariate analysis showed that high CTP, MELD, AIMS65 and PALBI scores were predictors of mortality and associated with decreased survival with high sensitivity and low specificity; while multivariate analysis showed that only AIMS56 was independently associated with mortality (p 0.004).\u0000 \u0000 \u0000 CTP, MELD, AIMS65 and PALBI scores are simple, bed side risk scores that can be used for the prediction of 6 months’ mortality after AVB in cirrhotic patients with high sensitivities and lower specificities.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49380279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MCP1, CRP and Procalcitonin as Novel Diagnostic Markers in Cirrhotic Patients with Spontaneous Bacterial Peritonitis","authors":"A. Mohamed, Wafaa Salah, Mohamed B Hassan, Hala H. Eldeeb, A. Adaroas, R. Khattab, Heba M. Abostate, M. Y. Soliman, E. Habba, S. Abd-Elsalam, Y. Abo-Amer","doi":"10.2174/18753183-v12-e2206270","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2206270","url":null,"abstract":"\u0000 \u0000 The aim of the study was to evaluate serum c-reactive protein (CRP), ascitic procalcitonin (PCT) and monocyte chemotactic protein-1 (MCP-1) in the diagnosis of spontaneous bacterial peritonitis (SBP) in cirrhotic patients.\u0000 \u0000 \u0000 \u0000 A cross-sectional analytic study that included 199 patients with decompensated cirrhosis (101 with SBP and 98 without SBP). Patients were classified according to Child-Pugh criteria. Ascitic PCT and MCP-1 were measured by enzyme-linked immunosorbent assay. Serum CRP, liver and renal functions were assessed.\u0000 \u0000 \u0000 \u0000 Three markers are significantly elevated in SBP patients than those without ascites. Using the ROC curve at AUC 0.883 and a cut-off value of >186 ng/ml, the diagnostic performance of ascitic MCP-1 level was higher than CRP (AUC 0.562) and ascitic fluid procalcitonin (AUC 0.751) in the diagnosis of SBP. The sensitivity and specificity were 86.15% and 79.59% at the cutoff of 186 ng/ml for MCP-1, 65.4 and 75.5 at ≥ 1 ng/ml for PCT, and 52.5 and 64.3, respectively for at 11.2 mg/dl CRP.\u0000 \u0000 \u0000 \u0000 Ascitic MCP-1 has a better diagnostic value with higher sensitivity and specificity in diagnosis SBP compared to CRP and procalcitonin which has higher diagnostic accuracy than CRP. Further studies with a large number will be necessary to evaluate the usefulness of these markers in diagnosis, follow-up and relation to morbidity and mortality of SBP patients.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44216917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin Sensitivity / Insulin Resistance as Predictors of Esophageal Varices in Post Chronic Hepatitis C Virus Patients","authors":"R. Badawi, M. Watany, Hala Moustafa Elsabagh, W. ElKhalawany","doi":"10.2174/18753183-v12-e2204040","DOIUrl":"https://doi.org/10.2174/18753183-v12-e2204040","url":null,"abstract":"\u0000 \u0000 Screening of Esophageal Varices (EV) in liver cirrhosis is highly recommended in all consensus reports. The standard screening procedure is endoscopy. Insulin resistance (IR) and the quantitative insulin sensitivity check index (QUICKI) are reliable predictors of portal hypertension.\u0000 \u0000 \u0000 \u0000 The study aimed to assess and compare the validity of insulin sensitivity/insulin resistance markers and other non-invasive markers for the detection of EVs in post chronic hepatitis C virus cirrhotic patients.\u0000 \u0000 \u0000 \u0000 In this cross-sectional study, 76 patients were screened by esophagogastroduodenoscopy and abdominal ultrasonography. Estimation of fasting serum insulin by ELISA technique was carried out. Homeostasis model assessment of insulin resistance (HOMA-IR) and QUICKI was performed.\u0000 \u0000 \u0000 \u0000 The patients with an advanced grade of EV had higher insulin resistance and lower QUICKI. A cut-off value of HOMA-IR ≥ 3.4 could significantly predict EVs with 72% sensitivity and 80.0% specificity. Spleen diameter and platelet count/spleen diameter ratio (PC/SD) showed a significant difference among groups.\u0000 \u0000 \u0000 \u0000 Lower insulin sensitivity (assessed by QUICKI) and higher insulin resistance (assessed by HOMA IR) were good non-invasive predictors of EVs. In addition, portal vein (PV) diameter, spleen diameter, and PC/SD were also found as predictors of EVs.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41660790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}