分离尿中游离DNA和细胞DNA的方法——甲基化特异性PCR在两种DNA片段上的应用

Q3 Medicine

Open Biomarkers Journal Pub Date : 2011-12-02 DOI:10.2174/1875318301104010015

Agnes Beermann, Foued Ghanjati, T. Hermanns, C. Poyet, J. Pereira, J. Fischer, P. Wernet, S. Santourlidis

{"title":"分离尿中游离DNA和细胞DNA的方法——甲基化特异性PCR在两种DNA片段上的应用","authors":"Agnes Beermann, Foued Ghanjati, T. Hermanns, C. Poyet, J. Pereira, J. Fischer, P. Wernet, S. Santourlidis","doi":"10.2174/1875318301104010015","DOIUrl":null,"url":null,"abstract":"The analysis of genomic DNA is widely-used for research, forensic and diagnostic purposes. Here we describe reliable methods for isolation of cell-free DNA and cellular DNA from urine. Both DNA fractions are suitable for PCR and Methylation-Specific PCR (MSP) amplification, leading to consistent and reproducible results. A kinetics analysis de- scribes the decline of efficiency of MSP performed with urinary DNA which had been stored at room temperature with and without proteinase K for various time periods.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"4 1","pages":"15-17"},"PeriodicalIF":0.0000,"publicationDate":"2011-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions\",\"authors\":\"Agnes Beermann, Foued Ghanjati, T. Hermanns, C. Poyet, J. Pereira, J. Fischer, P. Wernet, S. Santourlidis\",\"doi\":\"10.2174/1875318301104010015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The analysis of genomic DNA is widely-used for research, forensic and diagnostic purposes. Here we describe reliable methods for isolation of cell-free DNA and cellular DNA from urine. Both DNA fractions are suitable for PCR and Methylation-Specific PCR (MSP) amplification, leading to consistent and reproducible results. A kinetics analysis de- scribes the decline of efficiency of MSP performed with urinary DNA which had been stored at room temperature with and without proteinase K for various time periods.\",\"PeriodicalId\":39398,\"journal\":{\"name\":\"Open Biomarkers Journal\",\"volume\":\"4 1\",\"pages\":\"15-17\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-12-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Biomarkers Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1875318301104010015\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Biomarkers Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1875318301104010015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 4

摘要

基因组DNA的分析被广泛用于研究、法医和诊断目的。在这里，我们描述了从尿液中分离无细胞DNA和细胞DNA的可靠方法。这两个DNA片段都适用于PCR和甲基化特异性PCR (MSP)扩增，导致一致和可重复的结果。动力学分析描述了在室温下储存不同时间的有或没有蛋白酶K的尿液DNA进行MSP效率的下降。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions

The analysis of genomic DNA is widely-used for research, forensic and diagnostic purposes. Here we describe reliable methods for isolation of cell-free DNA and cellular DNA from urine. Both DNA fractions are suitable for PCR and Methylation-Specific PCR (MSP) amplification, leading to consistent and reproducible results. A kinetics analysis de- scribes the decline of efficiency of MSP performed with urinary DNA which had been stored at room temperature with and without proteinase K for various time periods.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Open Biomarkers Journal Medicine-Medicine (miscellaneous)

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The Open Biomarkers Journal is an Open Access online journal, which publishes original full-length, short research articles and reviews on biomarkers in clinical, medical and pharmaceutical research. The coverage includes biomarkers of disease, new biomarkers, exposure to drugs, genetic effects, and applications of biomarkers. The Open Biomarkers Journal, a peer reviewed journal, aims to provide the most complete and reliable source of information on current developments in the field. The emphasis will be on publishing quality articles rapidly and freely available to researchers worldwide.