Journal of Environmental Microbiology and Toxicology最新文献_第4页

Characterization of Butachlor Degradation by A Molybdenum-Reducing and Aniline-degrading Pseudomonas sp. 一种还原钼和苯胺的假单胞菌对丁草胺的降解特性

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.640

A. Sufyan, S. Ibrahim, A. Babandi, H. Yakasai

{"title":"Characterization of Butachlor Degradation by A Molybdenum-Reducing and Aniline-degrading Pseudomonas sp.","authors":"A. Sufyan, S. Ibrahim, A. Babandi, H. Yakasai","doi":"10.54987/jemat.v9i2.640","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.640","url":null,"abstract":"Butachlor is a chloroacetanilide herbicide that is selective in action and commonly used for pre-emergence control of weeds. It is believed to have range of toxicity from acute to chronic and also can cause DNA strand breaks and chromosomal aberrations in humans. This study was aimed at characterizing the potential of previously isolated bacteria for butachlor degradation. The isolates from culture collection, labelled A-F were screened for butachlor degradation on Bushnell Hass agar media with butachlor as a sole carbon source. Isolate A (molybdenum-reducing and aniline-degrading Pseudomonas sp.) was observed to grow best and tolerated the highest concentration of butachlor supplemented in the media after 72 h of incubation at 37 ℃. Characterization study revealed that the Pseudomonas sp. can utilize and grow with butachlor at optimum pH between 6.0 - 6.5, temperature between 30 – 37 ℃ and can tolerate up to 600 mg/L butachlor concentration with increase in growth as inoculum size increases. Additionally, this bacterial strain shows no lag phase regardless of the concentration of the herbicide used and reach its maximum growth after 24 h of incubation. The ability of this isolate to tolerate and utilize butachlor as sole carbon source makes it suitable for future bioremediation of this toxicant.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131985079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection Limit of the Four-Parameter Logistic Model for the Quantitative Detection of Serum Squamous Cell Carcinoma Antigenin Cervical Cancer Based on Surface Plasmon Resonance Biosensor 基于表面等离子体共振生物传感器的四参数Logistic模型定量检测宫颈癌血清鳞状细胞癌抗原的检出限

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.644

N. A. Masdor

{"title":"Detection Limit of the Four-Parameter Logistic Model for the Quantitative Detection of Serum Squamous Cell Carcinoma Antigenin Cervical Cancer Based on Surface Plasmon Resonance Biosensor","authors":"N. A. Masdor","doi":"10.54987/jemat.v9i2.644","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.644","url":null,"abstract":"Biochemical diagnostic procedures, such as protein binding, rely on biomolecular interactions as its diagnostic modality, and as a consequence, their calibration curves are more complex. In addition, sigmoidal curves are often seen in these tests. In the event of asymmetry, a logistic (5PL) curve, or a logistic (4PL) curve, may be the best way to represent a distinctive sigmoidal relationship. It is possible that the linearization of an otherwise nonlinear connection by log transformation may result in a disruption of the error structure of the curve, and that this will have the opposite effect of decreasing or even eliminating error in the relationship. Previously, a surface plasmon resonance biosensor for the detection of squamous cell carcinoma antigen (SCCa) using nanoparticle technology was developed. However, based on the calibration curve, it conforms to the majority on sigmoidal shape curve for antibody-type sensing system. The resultant curve showed a sigmoidal calibration curve but was not modelled according to any of the sigmoidal models available. The LOD value obtained through the 4PL modelling exercise based on the classical method was 0.255 pM (95% confidence interval of 0.167 to 0.379) while the pooled standard deviation (PSD) method yielded an LOD value of 0.035 pM (95% confidence interval of 0.011 to 0.067), which indicates that the PSD method was superior.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"509 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123200619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Arrhenius Plot Analysis, Temperature Coefficient and Q10 Value Estimation for the Effect of Temperature on the Rate of Molybdenum Reduction by Serratia marcescens strain DRY6 粘质沙雷氏菌DRY6菌株温度对钼还原速率影响的Arrhenius图分析、温度系数和Q10值估算

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-07-31 DOI: 10.54987/jemat.v9i1.600

A. Abubakar

{"title":"Arrhenius Plot Analysis, Temperature Coefficient and Q10 Value Estimation for the Effect of Temperature on the Rate of Molybdenum Reduction by Serratia marcescens strain DRY6","authors":"A. Abubakar","doi":"10.54987/jemat.v9i1.600","DOIUrl":"https://doi.org/10.54987/jemat.v9i1.600","url":null,"abstract":"Molybdenum is a micronutrient that serves as a co-factor for over 50 enzymes and catalyses a variety of hydroxylation and redox transfer activities in animal and plant physiology. When its concentration is elevated, it poses a great danger as it can interfere with the processes of spermatogenesis and oogenesis in several animals including fish. Its remediation using molybdenum-reducing bacterium is an emerging important tool. One of the parameters that are important in governing molybdenum reduction is temperature. Several models may be used to simulate the growth rate of microorganisms on various media as a function of temperature. Arrhenius is one of the very popular models since it has minimal parameters. Temperature generally affects microbial growth and metabolic activity on their substrates. The small nature of microbes makes them susceptible to change in the surrounding temperature. Growth on molybdenum by Serratia marcescens strain DRY6 is described, with a discontinuous chevron-like graph of apparent activation energy with a breakpoint at 26.25 oC. Regression analysis resulted in two activation energies: 15-25 oC and 30â€“40 oC with the activation energies of 52.7 kJ/mol and 13.33 kJ/mol, respectively. For the examined temperature range (15-25 oC), a Q10 value of 2.08 and a theta value of 1.02 was calculated. This is study is very useful in predicting the breakdown of molybdenum and the movement of molybdenum during bioremediation.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133651281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Arrhenius Plot Analysis, Temperature Coefficient and Q10 Value Estimation for the Effect of Temperature on Molybdenum Reduction Rate by Pantoea sp. strain HMY-P4 温度对Pantoea sp.菌株HMY-P4钼还原率影响的Arrhenius图分析、温度系数及Q10值估算

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-07-31 DOI: 10.54987/jemat.v9i1.599

H. Yakasai, A.J. Safiyanu, Slamet Ibrahim, A. Babandi

{"title":"Arrhenius Plot Analysis, Temperature Coefficient and Q10 Value Estimation for the Effect of Temperature on Molybdenum Reduction Rate by Pantoea sp. strain HMY-P4","authors":"H. Yakasai, A.J. Safiyanu, Slamet Ibrahim, A. Babandi","doi":"10.54987/jemat.v9i1.599","DOIUrl":"https://doi.org/10.54987/jemat.v9i1.599","url":null,"abstract":"The process of microbial molybdenum reduction to molybdenum blue precipitate is a biological phenomenon exploited in the bioremediation of molybdenum pollutant. This process is impacted by a change of the surrounding temperature. In general, to model the impact of small temperature ranges on the growth rate of microorganisms on substrates, several models are available. Arrhenius is one of the models and is very common because it has few parameters. An apparent activation energy based on the Arrhenius plot on the rate of molybdenum reduction by Pantoea sp. strain HMY-P4 is reported in this study. A linear plot of ln qmax against 1/T at the temperature range studied (25â€“40 oC) resulted in an activation energy value of 45.69 kJ per mol (95% Confidence Interval or C.I., 32.04 to 59.35). The plot also yields the Arrhenius frequency factor (A) which was calculated as 2.71 Ã— 107 (95% C.I., 1.25 Ã— 105 to 5.86 Ã— 109). The corresponding Q10 value of 2, which represents a relative 10 ËšC increase in the surrounding temperature and a doubling of the reaction rate. The Q10 value of 1.82 (95% C.I., 1.52 to 2.18) and a theta value of 1.06 (95% C.I., 1.05 to 1.08) was calculated. The values obtained in this work are within the normal range for many biological processes.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116273004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Limits of Detection Based on the Four-Parameter Logistic Model for E. coli Determined using a Fluorescent-based Sensor 基于四参数Logistic模型的荧光传感器检测大肠杆菌的检出限

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-07-31 DOI: 10.54987/jemat.v9i1.596

S. N. Padrilah, N. A. Masdor

{"title":"Limits of Detection Based on the Four-Parameter Logistic Model for E. coli Determined using a Fluorescent-based Sensor","authors":"S. N. Padrilah, N. A. Masdor","doi":"10.54987/jemat.v9i1.596","DOIUrl":"https://doi.org/10.54987/jemat.v9i1.596","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000Because of the huge number of outbreaks, bacterial infection is becoming more common, which is compounded by the rise of antibiotic resistance. These issues have warranted the development of sensitive detection and therapeutic devices. A fluorescence-based detection of the pathogen E. coli is previously developed using a mini-emulsion technique to create fluorescent/electroactive poly(3-hexylthiophene) P3HT nanoparticles (NPs) stabilized with CTAB or cetyltrimethylammonium bromide, a quaternary ammonium salt forming CTAB-P3HT NPs. Limits of detection for the bacterium based on fluorescence spectroscopy is 5 CFU/mL. The curve showed a sigmoidal calibration curve but was not modelled according to any of the sigmoidal models available. The aim of this study is to use the Four-Parameter Logistic Model to determine the LOD value more accurately. The modelling exercise gave values of the parameters a, d, Log EC50 and Hillslope representing the maximum and minimum responses, value that produces a 50% signal response, and a slope-like parameter (Hill coefficient) of -0.01427 (DF/F0), 0.6189 (DF/F0), 3.473 and 0.413, respectively. The LOD value was 30 CFU/mL with the 95% confidence interval from 13 to 64 CFU/mL. The usage of the 4Pl model in this study was successful, since it was able to represent the entire date curve. The correlation coefficient values of 0.986 indicated good fitting of the experimental data to the 4PL model. The 4PL model has been found to be a good model in fitting the calibration curve for the detection of E. coli. \u0000 \u0000 \u0000 \u0000","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134236172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimization of Culture Conditions for the Production of Alkaline Cellulase Enzyme Produced from Fusarium oxysporum VSTPDK 尖孢镰刀菌VSTPDK产碱性纤维素酶培养条件的优化

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-07-31 DOI: 10.54987/jemat.v9i1.597

A. Yakubu, G. Uba, A. Vyas

{"title":"Optimization of Culture Conditions for the Production of Alkaline Cellulase Enzyme Produced from Fusarium oxysporum VSTPDK","authors":"A. Yakubu, G. Uba, A. Vyas","doi":"10.54987/jemat.v9i1.597","DOIUrl":"https://doi.org/10.54987/jemat.v9i1.597","url":null,"abstract":"Alkaline cellulase producing Fusarium oxysporum VSTPDK was isolated and screened from the soil of Kapurthala district Punjab, India. This organism produced endoglucanase (CMCase) and exoglucanase (FPase) when grown at a different range of parameters pH (6, 7, 8, 9, 10 and 11), temperature (30, 40, 50 and 60OC), incubation time (4th 6th 8th 10th and 12th day) and nitrogen source (NH4SO4, NH4Cl, NaNO3 and NH4HCO3). Carboxymethylcellulose (CMC) and cellulose powder were used as the sole carbon source. In this research, statistical tools called Response Surface Methodology (RSM) was used for the optimization of cellulase enzyme by selecting three important parameters after one factor at a time (OFAT) approach. Using OFAT, optimum production of both CMCsase (3.52U/mL) and FPase (4.07U/mL) were achieved after 8 days incubation at pH 8, temperature 30OC and 1.0g/L ammonium sulphate while RSM produced CMCase 3.91U/mL and FPase 4.26U/mL respectively when incubated for 8 days at pH 8.5, temperature 45 and 3% ammonium sulphate concentration. Optimization of the culture conditions using RSM leads to an increase of 0.39U/mL (CMCase) and 0.19U/mL (FPase). The use of RSM has gained considerable attention in the past decade in the optimization of various physicochemical parameters and nutritional factors. Its application in different industries may find ways of selecting different factors influencing cellulase activity. The fungus can produce a considerable amount of cellulase enzyme at a pH of up to 10 and 50OC. To our knowledge, this is the first report of alkalothermophilic oxysporum VSTPDK from Punjab, India.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129468801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Modelling the Effect of Copper on the Growth Rate of Enterobacter sp. strain Neni-13 on SDS 铜对肠杆菌ni-13菌株在SDS上生长速率影响的模拟

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-07-31 DOI: 10.54987/jemat.v9i1.598

.. Rusnam, N. Gusmanizar, M. Shukor, Bilal Ibrahim Dan-Iya

{"title":"Modelling the Effect of Copper on the Growth Rate of Enterobacter sp. strain Neni-13 on SDS","authors":".. Rusnam, N. Gusmanizar, M. Shukor, Bilal Ibrahim Dan-Iya","doi":"10.54987/jemat.v9i1.598","DOIUrl":"https://doi.org/10.54987/jemat.v9i1.598","url":null,"abstract":"The introduction of tiny amounts of heavy metals into the environment can encourage the growth of a wide variety of microorganisms. The concentration at which enhanced microbial activity is seen, on the other hand, results in a significant decrease in growth rate as well as an increase in lag time (due to the higher lag time). An established link exists between heavy metal toxicity in microorganisms and the process of bioremediation, which has been well-documented. Because heavy metals have an impact on bioremediation, they must be researched, and appropriate countermeasures must be implemented. Copper reduced the growth of the SDS-degrading bacteria Enterobacter sp. strain Neni-13 to a significant extent. Under varying doses of mercury, the SDS-degrading bacteria exhibited a sigmoidal pattern with time periods ranging from 7 to 10 hours. Gompertz's model was used to calculate the growth rates of copper in different concentrations. As the copper concentration rose, the growth of bacteria was suppressed with a concentration of 1.0 g/L, with virtually total stoppage of bacterial development. From the Gompertz model, we got the estimates of growth rates; after which, they were estimated according to the Han-Levenspiel, Shukor, Wang, Liu, Andrews, and Amor models. The modified Han-Levenspiel, Andrews, Liu, and Shukor models could all successfully fit the curve. Results of the statistical analysis showed that the Han-Levenspiel model was the best model based on highest adjusted correlation coefficient (adR2), the lowest values for RMSE and AICc, and values of AF and BF closest to unity. The parameters obtained from the Han-Levenspiel model were Ccrit 0.209 mg/L (95%, C.I., 0.199 to 0.219), Î¼max 0.209 h-1 (95% C.I., 0.199 to 0.219) and m 0.472 (95% C.I., 0.383 to 0.561. The results obtained in this study indicate the maximum tolerable copper concentration that the conditions for biodegradation should not exceed.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131483837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Thermodynamic Study on the Biosorptive Removal of Lead (II) Ions from Aqueous Solutions using Acid-treated Cystoseira tricta Biomass 酸处理的三角囊藻生物质生物吸附去除水溶液中铅离子的热力学研究

Journal of Environmental Microbiology and Toxicology Pub Date : 2020-12-31 DOI: 10.54987/jemat.v8i2.563

Salihu Yahuza, I. Sabo, Abdussamad Abubakar, M. Shukor

{"title":"Thermodynamic Study on the Biosorptive Removal of Lead (II) Ions from Aqueous Solutions using Acid-treated Cystoseira tricta Biomass","authors":"Salihu Yahuza, I. Sabo, Abdussamad Abubakar, M. Shukor","doi":"10.54987/jemat.v8i2.563","DOIUrl":"https://doi.org/10.54987/jemat.v8i2.563","url":null,"abstract":"Thermodynamic studies on adsorption process play a vital role in the estimation of adsorptive mechanisms (both physical and chemical). Estimating the accuracy of thermodynamic parameters is dependent upon the equilibrium constants between two phases (KL: dimensionless). In this study, thermodynamic parameters were calculated from the KL constant derived from the adsorption of Langmuir isotherms of Pb(II) on an sulphuric acid-treated brown algae Cystoseira stricta biomass at different temperatures. The conversion of the KL values to the dimensionless Kc values based on the Langmuir model was then assessed for thermodynamic parameters via the Vanâ€™t Hoffâ€™s equation. The Pb(II) adsorption process onto the Cystoseira stricta biomass was spontaneous and feasible with DG values at 25, 30, 35 and 40 oC of -24.56 (95% C.I., -24.00 to -25.03), -22.60 (95% C.I.,-21.95 to -23.12), -20.90 (95% C.I.,-18.87 to -22.01) and -17.17 (95% C.I.,-17.71 to -21.41), respectively, and occurred in an endothermic nature (DH= -0.30 kJ/mol (95% C.I., -194.34 to 193.74) with an increased in randomness (DS= -299.47 J/molÃ—K (95% C.I., -553.60 to -45.35) kJ/mol). To confirm the accuracy and precision of the asymptotatic 95% confidence interval, Monte Carlo simulation may be carried out in the future.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"os-47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127785338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mathematical Modeling of the Molybdenum Blue Production from Serratia sp. strain DRY5 沙雷氏菌株 DRY5 生产钼蓝的数学建模

Journal of Environmental Microbiology and Toxicology Pub Date : 2020-12-31 DOI: 10.54987/jemat.v8i2.565

M. Syed, N. A. Shamaan, M. Shukor

{"title":"Mathematical Modeling of the Molybdenum Blue Production from Serratia sp. strain DRY5","authors":"M. Syed, N. A. Shamaan, M. Shukor","doi":"10.54987/jemat.v8i2.565","DOIUrl":"https://doi.org/10.54987/jemat.v8i2.565","url":null,"abstract":"The reduction of molybdenum to molybdenum blue is a detoxification process and the development of Mo-blue is associated with growth. Significant parameters such as precise reduction rate, theoretical maximum reduction and whether reduction at high molybdenum concentration influenced the lag time of reduction can be discovered by mathematical modeling of the reduction phase. While common, the use of the linearization method by the use of natural logarithm transformation is inaccurate and can only provide an approximate value for the calculated single parameter, the real growth rate. In this work, a variety of models for such as logistic, Gompertz, Richards, Schnute, Baranyi-Roberts, Von Bertalanffy, Buchanan three-phase and more recently Huang were utilized to obtain values for the above parameters or constants. The Huang model was the best model in modelling the Mo-blue production curve of the Serratia sp. strain DRY5 based on statistical tests such as root-mean-square error (RMSE) (0.043), adjusted coefficient of determination (R2) (0.994), bias factor (BF) (1.00), accuracy factor (AF) (1.03) and corrected AICc (Akaike Information Criterion) (-67.02). Parameters obtained from the fitting exercise were maximum Mo-blue production rate (ïm), lag time (ï¬) and maximal Mo-blue production (Ymax). We make use of primary growth models for modeling Mo-blue output in this work. This is an emerging method of identifying constants of parameters that control the reduction of molybdenum. For the creation of further secondary models, the constants calculated from this work will be utilized for such purpose. The use of these primary models can also be broadened to processes involving the detoxification of other heavy metals.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127748187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Modelling the Kinetics Molybdenum Reduction Rate by Morganella sp. 摩根菌对钼还原速率的动力学模拟。

Journal of Environmental Microbiology and Toxicology Pub Date : 2020-12-31 DOI: 10.54987/jemat.v8i2.566

H. Yakasai, A. Babandi, M. Manogaran

{"title":"Modelling the Kinetics Molybdenum Reduction Rate by Morganella sp.","authors":"H. Yakasai, A. Babandi, M. Manogaran","doi":"10.54987/jemat.v8i2.566","DOIUrl":"https://doi.org/10.54987/jemat.v8i2.566","url":null,"abstract":"Simulation of physical, chemical or biological data by mathematical approach can aid the researcher explain the physicochemical or biochemical phenomenon. Mathematical models can also be utilized to estimate or predict the possible actions, to replicate a prediction or retroactivity and to schedule better experiments. Bacteria are an emerging instrument for the bioremediation of the molybdenum pollutant in the world, molybdenum reduction into insoluble molybdenum blue. In this study, the molybdenum reduction rate by the bacterium Morganella sp. was studied for its inhibitory kinetics characteristics using sodium molybdate as a substrate. Modeling kinetics showed that molybdenum reduction could be explained by several models such as Monod, Haldane, Hans-Levenspiel, Luong, Teissier, Aiba and Yano with Aiba as the best model as judged using error function analysis such as bias and accuracy factors (BF and AF), root mean square error (RMSE), adjusted coefficient of determination (adjR2), and corrected Akaike Information Criterion (AICc). The calculated value for the Teissier-Edwardâ€™s constants, which are qmax, Ks, and Ki that are maximal reduction rate, half saturation constant for maximal reduction, half saturation constant for inhibition of reduction were 7.77 (95% C.I., 4.41 to 19.95) Âµmol Mo-blue hr-1, 26.63 (95% C.I., 12.82 to 40.44) mM and 51.39 (95% C.I., 23.67 to 79.10) mM, respectively. The true maximal reduction rate, which occurred when the slope of the curve is zero occurs at 36 mM molybdate concentration and a corresponding value of 1.85 Âµmol Mo-blue hr-1. The model parameters obtained revealed that Morganella sp. have strong resistance to higher toxic molybdenum levels indicating the usefulness of this bacterium for bioremediation.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"234 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132824550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1