一种还原钼和苯胺的假单胞菌对丁草胺的降解特性

A. Sufyan, S. Ibrahim, A. Babandi, H. Yakasai
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引用次数: 1

摘要

丁草胺是一种选择性的氯乙酰胺类除草剂,通常用于杂草的苗期控制。据信,它的毒性范围从急性到慢性,也可以导致人类DNA链断裂和染色体畸变。本研究的目的是表征以前分离的细菌降解丁草胺的潜力。在以丁草胺为唯一碳源的Bushnell - Hass琼脂培养基上,对标记为a - f的培养物进行了丁草胺降解筛选。分离菌株A(钼还原和苯胺降解假单胞菌sp.)在37℃培养72 h后,在培养基中添加最高浓度的丁草胺,生长最好,耐受性最强。特性研究表明,在pH 6.0 ~ 6.5、温度30 ~ 37℃的条件下,假单胞菌对丁草胺的耐受性可达600 mg/L,且随着接种量的增加,其耐受性也随之增加。此外,无论除草剂浓度如何,该菌株均不存在滞后期,在孵育24 h后达到最大生长。该分离物具有耐丁草胺和利用丁草胺作为唯一碳源的能力,适合未来对该毒物进行生物修复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Butachlor Degradation by A Molybdenum-Reducing and Aniline-degrading Pseudomonas sp.
Butachlor is a chloroacetanilide herbicide that is selective in action and commonly used for pre-emergence control of weeds. It is believed to have range of toxicity from acute to chronic and also can cause DNA strand breaks and chromosomal aberrations in humans. This study was aimed at characterizing the potential of previously isolated bacteria for butachlor degradation. The isolates from culture collection, labelled A-F were screened for butachlor degradation on Bushnell Hass agar media with butachlor as a sole carbon source. Isolate A (molybdenum-reducing and aniline-degrading Pseudomonas sp.) was observed to grow best and tolerated the highest concentration of butachlor supplemented in the media after 72 h of incubation at 37 ℃. Characterization study revealed that the Pseudomonas sp. can utilize and grow with butachlor at optimum pH between 6.0 - 6.5, temperature between 30 – 37 ℃ and can tolerate up to 600 mg/L butachlor concentration with increase in growth as inoculum size increases. Additionally, this bacterial strain shows no lag phase regardless of the concentration of the herbicide used and reach its maximum growth after 24 h of incubation. The ability of this isolate to tolerate and utilize butachlor as sole carbon source makes it suitable for future bioremediation of this toxicant.
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