Journal of Environmental Microbiology and Toxicology最新文献_第3页

Activation energy, Temperature Coefficient and Q10 Value Estimations of the Growth of Serratia marcescens strain DRY6 on SDS 粘质沙雷菌DRY6菌株在SDS上生长的活化能、温度系数和Q10值估算

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.695

Abubakar Aisami

{"title":"Activation energy, Temperature Coefficient and Q10 Value Estimations of the Growth of Serratia marcescens strain DRY6 on SDS","authors":"Abubakar Aisami","doi":"10.54987/jemat.v10i1.695","DOIUrl":"https://doi.org/10.54987/jemat.v10i1.695","url":null,"abstract":"Sodium dodecyl sulfate, occasionally known as SDS, is a widely used anionic surfactant globally as a foaming component in a wide variety of cleaning products, including shampoos, toothpaste, and detergents. Large quantities of SDS are released into the environment despite the fact that they are hazardous and have the ability to create foam. This can lead to problems in sewage treatment facilities. There are a few different models that may be utilized to mimic the growth rate of microbes on a variety of different medium at various temperature. Arrhenius is one of the most often used models partly since it has a small number of parameters. Temperature frequently influences the progress and metabolic activities of microbes on the substrates they are growing on. Because of their small size, microbes are very sensitive to variations in temperature. In order to describe the development of Serratia marcescens strain DRY6 on SDS, a discontinuous apparent activation energy with a chevron-like graph was used, and the graph's break point was set at 28.05 degrees Celsius. Following the completion of the regression study, two activation temperatures were established: 20-27 degrees Celsius and 30-42 degrees Celsius, with respective activation energies of 41.72 and 84.72 kilojoules per mole. Within the temperature range that was taken into account, it was projected that the Q10 value would be 2.905, and the theta value would be 1.11. (30-42 oC). This study is especially helpful in projecting SDS breakdown and migration during bioremediation because of its comprehensive nature.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125438647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isothermal Modelling of the Adsorption of Lead(II) Onto an Antarctic Sea-Ice Bacterial Exopolysaccharide 南极海冰细菌胞外多糖吸附铅(II)的等温模拟

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.694

Muhammed Abdullahi Ubana, M. Ya’u, Hartinie Marbawi, N. A. Yasid, M. Shukor

{"title":"Isothermal Modelling of the Adsorption of Lead(II) Onto an Antarctic Sea-Ice Bacterial Exopolysaccharide","authors":"Muhammed Abdullahi Ubana, M. Ya’u, Hartinie Marbawi, N. A. Yasid, M. Shukor","doi":"10.54987/jemat.v10i1.694","DOIUrl":"https://doi.org/10.54987/jemat.v10i1.694","url":null,"abstract":"The biosorption of the biosorption of lead(II) onto an Antarctic sea-ice bacterial exopolysaccharide is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Sips model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc) which is not the same to the originally published work using a linearized form where the Langmuir and Freundlich models best represent the biosorption and the maximum biosorption capacity. The calculated Sips parameters kS (l/g) value of 0.10 (95% confidence interval from 0.08 to 0.13), a maximum monolayer adsorption capacity qmS (mg/g) value of 252.88 (95% C.I. from 222.13 to 283.64) and nS (Sips model exponent) value of 1.16 (95% C.I. from 1.34 to 1.98). This study indicates that a different isotherm model can be obtained using nonlinear regression compared to the popular linearized form that may give relatively inaccurate outcome.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128287900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Biomonitoring of Heavy Metals in Polluted Sites Using Xenoassay®-Metal 利用Xenoassay®-Metal快速监测污染场地中的重金属

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.688

M. F. Rahman, M. Manogaran, M. Shukor

{"title":"Rapid Biomonitoring of Heavy Metals in Polluted Sites Using Xenoassay®-Metal","authors":"M. F. Rahman, M. Manogaran, M. Shukor","doi":"10.54987/jemat.v10i1.688","DOIUrl":"https://doi.org/10.54987/jemat.v10i1.688","url":null,"abstract":"Long-term water contamination, including heavy metals, may harm the reputation of the location. Because of this, it is necessary to do regular water quality checks. In light of the high expense of instrument monitoring, biomonitoring based on enzymes is rapidly gaining popularity around the world. For the purpose of this investigation, a molybdenum-reducing enzyme from the Serratia marcescens strain DRY6 that is sensitive to the heavy metals was used to build a quick inhibitory enzyme assay. The bacterium's molybdenum-reducing activity is extremely vulnerable to heavy metals. With the help of the established assay, heavy metals were detected in water samples collected in Malacca, Penang, and Perak. A number of sampling locations in Penang were found to be inhibiting enzyme activity, while all sampling locations in Malacca and Penang were confirmed to be free of high heavy metal concentrations. The Malaysian Department of Environment has set a maximum permissible limit for the suppression of Mo-reducing enzyme activity in Penang. Detection of heavy metals can be done in less than ten min at room temperature. The entire procedure takes less than 20 min. a first screening procedure or even a near real-time method for routine monitoring of heavy metals, assay is quick and straightforward.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"775 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126949998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Test of the Randomness of Residuals and Detection of Potential Outliers for the Modified Logistics Used in the Fitting of the Growth Curve of Immobilized Pseudomonas putida on Phenol 固定化恶臭假单胞菌在苯酚上的生长曲线拟合中残差随机性检验及潜在异常值检测

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.692

G. Uba, M. Ya’u

{"title":"Test of the Randomness of Residuals and Detection of Potential Outliers for the Modified Logistics Used in the Fitting of the Growth Curve of Immobilized Pseudomonas putida on Phenol","authors":"G. Uba, M. Ya’u","doi":"10.54987/jemat.v10i1.692","DOIUrl":"https://doi.org/10.54987/jemat.v10i1.692","url":null,"abstract":"As a result of the fact that several research do not carry out statistical diagnostics on the nonlinear model that was employed, the data could not be random. Because these systems rely on random data, this is a necessity for all parametric statistical assessment procedures. The Wald–Wolfowitz runs test was done on the modified logistics that were employed in the fitting of the growth curve of immobilized Pseudomonas putida on phenol. This test was carried out in order to determine whether or not the logistical changes had any effect on the growth curve. This test was carried out so that it could be determined whether or not the adjustments made to the logistical processes were successful. The runs test showed that there was a total of eight runs, which contradicts the expectation that there would only be seven runs due to the unpredictability of the circumstance. The assumption was based on the fact that there would only be seven runs. Since the p-value was larger than 0.05, the null hypothesis is not rejected; this suggests that there is no convincing evidence of the non-randomness of the residuals; rather, the residuals represent noise in the data. As a consequence of the findings of Grubb's test, which indicate that there is no outlier, it is not necessary to reanalyze the data because the modified logistics model used in the fitting of the growth curve of immobilized Pseudomonas putida on phenol was adequate enough. This means that the reanalysis would be unnecessary.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128634959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetic Analysis of the Adsorption of Cadmium onto Activated Carbon from Tridax procumbens 活性炭吸附镉的动力学分析

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.691

Faggo Abdullahi Adamu, Khalizan Sabullah, Umar Abubakar Muhammad, N. A. Yasid, M. Shukor

{"title":"Kinetic Analysis of the Adsorption of Cadmium onto Activated Carbon from Tridax procumbens","authors":"Faggo Abdullahi Adamu, Khalizan Sabullah, Umar Abubakar Muhammad, N. A. Yasid, M. Shukor","doi":"10.54987/jemat.v10i1.691","DOIUrl":"https://doi.org/10.54987/jemat.v10i1.691","url":null,"abstract":"While Cd production, consumption, and environmental release have all skyrocketed in recent decades, there is currently no practical way to recycle Cd compounds. This suggests that Cd compounds may be very dangerous to human health. Due to this cadmium remediation from polluted environment is urgently needed. Several viable technologies include biosorption, which has several positive aspects which include low operating expenses, very efficient detoxification of toxicants at low concentrations and low amount of disposal materials. The biosorption of the biosorption of cadmium onto the activated carbon from Tridax procumbens is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. Statistical analysis showed that the best kinetic model for adsorption was pseudo-1st order. All error function analyses supported the pseudo-1st order model. The pseudo-1st order kinetic constants obtained were qe (mg/g) of 5.808 (95% confidence interval from 5.043 to 6.573) and k1 (per min) of 0.009 (95% confidence interval from 0.0064 to 0.0117). Nonlinear modeling enables the determination of a 95 percent confidence interval for the uncertainty range, which can be used in model comparison and discriminant analysis.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"194 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123401120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of a PEG-degrading and Mo-reducing Escherichia coli strain Amr-13 in soils from Egypt 埃及土壤中降解聚乙二醇和还原钼的大肠埃希菌Amr-13的分离与鉴定

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.643

Nubli Shuhaimi, M. AbdEl-Mongy, N. A. Shamaan, Chaing Hin Lee, M. Syed, M. Shukor

{"title":"Isolation and Characterization of a PEG-degrading and Mo-reducing Escherichia coli strain Amr-13 in soils from Egypt","authors":"Nubli Shuhaimi, M. AbdEl-Mongy, N. A. Shamaan, Chaing Hin Lee, M. Syed, M. Shukor","doi":"10.54987/jemat.v9i2.643","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.643","url":null,"abstract":"Molybdenum is a pollutant that shows toxicity to spermatogenesis while polyethylene glycols (PEG) are used predominantly in detergents. The pollution of molybdenum and PEGs are reported worldwide. We have isolated ten molybdenum-reducing bacterial isolates from soil that can reduce molybdenum (sodium molybdate) into the colloidal molybdenum blue (Mo-blue). The screening of these isolates for PEG-degrading ability showed that one isolate was capable to utilize PEG 200, 300 and 600 for optimal conditions were pHs between 5.5 and 8.0, temperatures between 30 and 37 oC, phosphate at 5 mM, molybdate between 10 and 30 mM, and glucose as the electron donor. Biochemical analysis of the bacterium identifies it as Escherichia coli strain Amr-13. Growth was best supported by all PEGs at concentrations of between 600 and 1,000 mg/L. A complete degradation for PEG 200 and PEG 300 at 1,000 mg/L was observed on day four and five, respectively, while nearly 90% of PEG 600 was degraded on day six. The growth of this bacterium on these PEGs was modelled using the modified Gompertz model, and produced growth parameters values, which were maximum specific growth rates of 1.51, 1.45 and 1.18 d-1 and lag periods of 0.53, 0.87 and 1.02 day for PEG 200, PEG 300 and PEG 600, respectively. PEG 200 was the most preferred substrate for this bacterium, while PEG 600 was the least preferred.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130625202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Kinetics Modeling on the Biosorption of Remazol Black B Dye by Aspergillus flavus 黄曲霉对雷马唑黑B染料的生物吸附动力学模拟

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.642

Salihu Yahuza, I. Sabo, Hadiza Aliyu Biu

{"title":"Kinetics Modeling on the Biosorption of Remazol Black B Dye by Aspergillus flavus","authors":"Salihu Yahuza, I. Sabo, Hadiza Aliyu Biu","doi":"10.54987/jemat.v9i2.642","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.642","url":null,"abstract":"Azo dyes, such as Remazol Black B, are different from conventional dyes in that they establish covalent bonds with textile fibers like cotton. They are widely utilized in the textile industry because of their favorable properties of bright color, water resistance, simple application procedures, and low energy consumption. Their discharge into receiving streams has major environmental consequences, such as reducing photosynthesis in aquatic life due to lower light penetration. The biosorption isotherm data of Remazol Black B dye biosorption by Aspergillus flavus were investigated using two models—pseudo-1st order and pseudo-2nd order—and fitted using non-linear regression. The pseudo-1st order model was found to be the best by statistical analysis using root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), corrected AICc (Akaike Information Criterion), Bayesian Information Criterion (BIC), and Hannan–Quinn information criterion (HQC). At 250 mg/L, kinetic analysis using the pseudo-1st order model yielded an equilibrium sorption capacity qe of 4.61 mg/g (95 % confidence interval from 4.54 to 4.68) and a pseudo-1st-order rate constant, k1 of 0.15 (95% C.I. from 0.128 to 0.164).","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125407316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetics Modelling of Pseudomonas stutzeri strain DN2 Growth Behaviour in Tributyltin Chloride stutzeri假单胞菌DN2在氯化三丁基锡中生长的动力学模型

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.641

Abdussamad Abubakar, G. Uba, Hadiza Aliyu Biu

{"title":"Kinetics Modelling of Pseudomonas stutzeri strain DN2 Growth Behaviour in Tributyltin Chloride","authors":"Abdussamad Abubakar, G. Uba, Hadiza Aliyu Biu","doi":"10.54987/jemat.v9i2.641","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.641","url":null,"abstract":"A predictive model was performed to describe Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride, using primary Modelling and a polynomial model as a secondary predictive model. In this investigation, data predicted using the modified Logistic (ML) was the most accurate. The Bias Factor (Bf) and Accuracy Factor (Af) values for the (ML) model were 1.39 and 1.51, indicating that the predictions were within a reliable range. The low RMSE value of 0.14, R2 and adj R2 (0.99) value closer to 1, showing that modified logistics is better than the other models at describing the growth behaviour of Pseudomonas stutzeri strain DN2 in toxic tributyltin chloride. Both the Aiba and Haldane models on the other hand, among the secondary model best fit the behaviours having low RMSE and MSE values and adjR2 value closer to 1. In this study, the primary and secondary kinetics of Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride was explored and it was shown in this study that the modified logistic and the Haldane models better suit the growth behavior of Pseudomonas stutzeri strain DN2 in tributyltin chloride. The parameters obtained from the modelling exercise will be very valuable in transferring the laboratory results to the field.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122575078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Effect of High Dosage of Codeine-Containing Cough Syrup on Renal Function on Albino Rats 大剂量含可待因止咳糖浆对白化病大鼠肾功能的影响

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.646

Ramlatu Musa Adam, Aishatu Muhammad Bello, Jalil Idi James, Alhassan Ahmad Siddan, A. Abubakar

{"title":"The Effect of High Dosage of Codeine-Containing Cough Syrup on Renal Function on Albino Rats","authors":"Ramlatu Musa Adam, Aishatu Muhammad Bello, Jalil Idi James, Alhassan Ahmad Siddan, A. Abubakar","doi":"10.54987/jemat.v9i2.646","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.646","url":null,"abstract":"Codeine-containing cough syrup (CCS) is one of the most abused drugs in the world especially among the youths. However, there is need to study the nephrotoxic effect associated with oral administration of the drug and to ascertain its effect on the kidney. Consequently, understanding the renal abnormalities in chronic use of CCS will be crucial for effective development of interventions. This study assessed the nephrotoxic effect associated with oral administration of codeine-containing cough syrup (Tutolin with Codeine) in albino rats, using the level of creatinine, urea, sodium, potassium and chloride ions as biomarker in the serum of albino rats. The rats were administered orally with Tutolin and Codeine at a dose of 80mg/kg, 160mg/kg, 240 mg/kg and 320mg/kg body weight. After three weeks of oral administration of the syrup to all the groups, there was no difference (P> 0.05) in the levels of sodium ion, chloride ion and creatinine among all the study groups and levels of urea and potassium ion in the group administered with 80 mg/kg, 160 mg/kg and 240 mg/kg body weight of tutolin with codeine compared with the control. After three weeks of oral administration of 80 mg/kg, 160 mg/kg, 240 mg/kg and 320 mg/kg body weight tutolin with codeine cough syrup, urea and potassium ion concentrations were higher (p<0.05) in group given 320mg/kg body weight of tutolin with codeine cough syrup compared with the control. This suggests that at higher doses, tutolin with codeine containing cough syrup may have effect on the kidney.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130312867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Nigerian Walnut (Juglan nigra) Lectin on Haematological Parameters and Lethal Dose (LD50) on Swiss Wistar 尼日利亚核桃凝集素对瑞士Wistar血液学参数和致死剂量(LD50)的影响

Journal of Environmental Microbiology and Toxicology Pub Date : 2021-12-31 DOI: 10.54987/jemat.v9i2.645

L. Alexander, U. Obeta, Michael Okungbowa, I. Emeje, Jonathan Sabulu, Gumta Rapheal Matthew, P. Agbalaka, Ezeanyagu Oluchi

{"title":"Effects of Nigerian Walnut (Juglan nigra) Lectin on Haematological Parameters and Lethal Dose (LD50) on Swiss Wistar","authors":"L. Alexander, U. Obeta, Michael Okungbowa, I. Emeje, Jonathan Sabulu, Gumta Rapheal Matthew, P. Agbalaka, Ezeanyagu Oluchi","doi":"10.54987/jemat.v9i2.645","DOIUrl":"https://doi.org/10.54987/jemat.v9i2.645","url":null,"abstract":"This study was designed to investigate the effects of Juglan nigra lectin on haematological parameters and lethal dose of rats. An authenticated Juglan nigra (UBHP401) collected from Lokoja was used to extract lectin and treated on Swiss Wistar rats (n=35) and (n=17) with weights from 75-129g for haematological parameters and LD50 respectively. The Wistar rats were divided into five equal groups labeled A, B, C, D, and E. Lectin concentrations were prepared and administered at stipulated days. Group A served as control, group B received 30%, C received 50%, D received 70% and group E received 90% of lectin intraperitoneally. Samples (Baseline-BL, First Harvests-FH and Second Harvest-SH) were collected from all the groups and analyzed for the following haematological parameters: white blood count (WBC), Red Blood count (RBC), Haemoglobin (HB), packed cell volume (PCV), platelet count, neutrophil, lymphocyte, monocytes, eosinophil and basophil. The data were analysed using two-way analysis of variance (ANOVA) in graph pad prism version 5.03. The Eosinophil count and white blood cell count were significantly increased P<0.005 and 0.05, whereas the HB, PCV and RBC showed significant different P<0.05 based on the day of exposure of the animal to the lectin, the platelet count was significantly increased on the basis of days of exposure of P<0.05, Eosinophil significantly increased too. Juglan nigra, (Nigerian walnut) Lectin is nontoxic as noted from LD50 result. Nigerian walnut lectin has a positive effect in haematopoietic activity of Swiss Wistar rats.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"438 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122885226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1