Journal of Environmental Microbiology and Toxicology最新文献_第2页

Glyphosate Biodegradation by Molybdenum-Reducing Pseudomonas sp. 还原钼假单胞菌降解草甘膦的研究。

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.772

Fatima Ibrahim Aliyu, A. Ibrahim, A. Babandi, Dayabbu Shehu, M. Ya’u, K. Babagana, S. Ibrahim, N. Abdullahi, A.J. Safiyanu, H. Yakasai

{"title":"Glyphosate Biodegradation by Molybdenum-Reducing Pseudomonas sp.","authors":"Fatima Ibrahim Aliyu, A. Ibrahim, A. Babandi, Dayabbu Shehu, M. Ya’u, K. Babagana, S. Ibrahim, N. Abdullahi, A.J. Safiyanu, H. Yakasai","doi":"10.54987/jemat.v10i2.772","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.772","url":null,"abstract":"Bioremediation of pollutants, such as herbicides, is an economic and environmentally friendly process. Glyphosate is an active ingredient in most herbicides utilized for weed control and desiccation on cereal and other grain crops globally. Glyphosate pollution poses a threat to the environment and the habitats in it. In this study, an isolated molybdenum-reducing bacterium was characterized for its potential to degrade glyphosate and utilized as the sole source of carbon and electron donor. The effects of incubation time, glyphosate concentration (carbon source), inoculum size, pH, temperature, aeration and heavy metals on the growth of this bacterium were spectrophotometrically assayed as OD600 nm. The bacterium degrades glyphosate faster under shaking conditions, optimally at pH 7.0, concentration 1.0 g/L, temperature 40 ºC, and inoculum size 400 µL. Growth of this bacterium was significantly inhibited by heavy metals in the order of Cu>Zn>Pb>Hg>Ag>Fe compared to the control. Glyphosate can serve as an electron donor source in hexavalent molybdenum reduction, but poorly supports molybdenum blue (Mo-blue) production compared to glucose. The dual role of this isolate as a metal reducer and glyphosate degrader makes it unique already and an important instrument for the bioremediation of mixed pollutants.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130394040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling the Kinetics of Tartrazine Sorption by Bottom Ash 底灰吸附酒黄石的动力学模拟

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.773

N. A. Yasid, A. A. Basirun, Hartinie Marbawi

{"title":"Modelling the Kinetics of Tartrazine Sorption by Bottom Ash","authors":"N. A. Yasid, A. A. Basirun, Hartinie Marbawi","doi":"10.54987/jemat.v10i2.773","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.773","url":null,"abstract":"Bottom ash is the solid residue left over from municipal waste combustion or incineration in a Municipal Waste Incineration Furnace. Its use as a sorption agent, particularly for dye sorption, is a new and important application. Linearized adsorption kinetics has drawbacks such as inaccurate representation of the parameters' 95 percent confidence interval output, unbalanced attention to potential outliers, and magnification of errors may result in inaccurate parameter values. In this study, we used nonlinear regression to investigate 16 adsorption kinetics models of tartrazine by bottom ash. The pseudo-second order was the best model based on the Bias and Accuracy factor near unity, but based on other error function analysis, this model performs equally well with the exponential and fractal-like pseudo-second order based other error functions such as Root-Mean-Square Error (RMSE), adjusted coefficient of determination (adjR2), Marquardt’s percent standard deviation (MPSD), Bayesian Information Criterion (BIC), Hannan-Quinn Information Criterion (HQC), and especially the corrected Akaike Information Criterion (AICc) function as the absolute difference is 5 absolute unit making discriminatory activity difficult. Furthermore, because the pseudo-second order and exponential models have only two parameters, they are less complicated according to Occam's razor. Because the pseudo-second order model is more popular and has more applications than the less well-known exponential model, we chose it as the best model for tartrazine sorption to bottom ash. Kinetic analysis using the PSO model gave a value of equilibrium adsorption capacity, qe of 21.88 mg g-1 (95% confidence interval (C.I.), 20.93 to 22.84) and k2 (g/(mg.sec)) of 0.00002 (95%, C.I., 0.00001 to 0.00002).","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131704469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of the Growth of Pseudomonas sp. strain DrY135 on Acrylamide 假单胞菌DrY135在丙烯酰胺上的生长特性

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.734

M. F. Rahman, M. E. Khayat, H. M. Yakasai, N. A. Yasid, M. Shukor

{"title":"Characterization of the Growth of Pseudomonas sp. strain DrY135 on Acrylamide","authors":"M. F. Rahman, M. E. Khayat, H. M. Yakasai, N. A. Yasid, M. Shukor","doi":"10.54987/jemat.v10i2.734","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.734","url":null,"abstract":"This study investigated the growth properties of a molybdenum-reducing bacteria previously isolated for its ability to break down amides. The bacterial growth range is 500–1000 mg/L, 6.5–8.0 pH, and 30–35 °C. The presence of hazardous heavy metals such as mercury, silver, and copper impeded this bacterium's development on acrylamide. The protracted lag phase seen when growing on acrylamide demonstrates the compound's severe growth inhibition. This bacterium has the potential to be an effective acrylamide bioremediation agent due to its greater tolerance for acrylamide than other acrylamide-degrading bacteria identified in the scientific literature. The influence of initial pH on bacterial growth at room temperature indicates that the optimal pH range lies between 6.5 and 8.0. The ideal temperature range for plant growth was between 30 and 35 oC. In a series of experiments utilizing a starting concentration of 1% (w/v) of various organic carbon sources, it was determined that glucose supported the most cellular growth on acrylamide, followed by sucrose, fructose, mannose, and citrate, in descending order of efficiency, whereas mannitol did not support growth. Doses of 300 and 500 mg/L of acrylamide stimulated the most rapid growth expansion, but concentrations of 1500 mg/L and above completely halted development. Copper (Cu), lead (Pb), cadmium (Cd), chromium (Cr), and mercury (Hg) were investigated at a concentration of 2 ppm. Mercury hindered growth by 71 percent, copper by 72 percent, and cadmium by 52 percent, according to our findings. There was a linear association between the acrylamide content and the delay before this bacterium began to develop. A lag time of one to three days was found when the acrylamide content grew from 100 to 1,500 mg/L. As quantities of acrylamide increased, so did the maximal growth rate, indicating an overall pattern of increasing toxicity.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115091393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Bacteria Obtained from Private Well Water within Akungba-Akoko Akungba-Akoko地区私人井水细菌的评价

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.736

O. R. Adeoyo, J.O. Omaku

{"title":"Evaluation of Bacteria Obtained from Private Well Water within Akungba-Akoko","authors":"O. R. Adeoyo, J.O. Omaku","doi":"10.54987/jemat.v10i2.736","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.736","url":null,"abstract":"Availability of potable water is important for healthy living. Domestic water is usually supplied to homes through private wells, boreholes and public water companies. This study aimed at evaluating bacteria from private well water samples which serves as a major water source in the study area (Akungba-Akoko). Samples were subjected to total bacterial and coliform counts using nutrient agar and eosin-methylene blue (EMB) respectively. All isolates were identified based on their morphological and biochemical characteristics. This was followed by antibiotics sensitivity test (AST). The result showed that total bacterial count ranged from 4.0 ×103 CFU/mL to 22.5 × 103 CFU/mL while total coliform count ranged from 1.0 × 100 CFU/mL to 7 × 100 CFU/mL. Gram positive bacteria belonging to the following genera; Bacillus, Corynebacterium, Enterococcus, Micrococcus, and Staphylococcus were obtained, while Gram negative bacteria include; Alcaligenes, Campylobacter, Citrobacter, Enterobacter, Escherichia, Klebsiella, Pseudomonas and Salmonella. Enterobacter and Pseudomonas had the highest percentages of occurrence of 15%. P. pseudo-mallei had the highest sensitivity to ofloxacin (23 mm), followed by P. fluorescens (22 mm) and C. freundii (22 mm) while B. subtilis was susceptible to ciprofloxacin, erythromycin, levofloxacin. The result showed high level of bacterial contamination in all samples tested. The coliform count of all water samples exceeded the recommended level of zero. Hence, there is the need for proper wells water maintenance, control and improve hygienic practices by the households and individuals to help solve the risk of disease outbreak.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126696475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of Used Engine Oil by Pseudomonas sp. Isolated from an Automobile Workshop’s Soil 某汽车车间土壤中假单胞菌降解废旧机油的研究

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.774

A. Muhammad, Faggo Abdullahi Adamu

{"title":"Biodegradation of Used Engine Oil by Pseudomonas sp. Isolated from an Automobile Workshop’s Soil","authors":"A. Muhammad, Faggo Abdullahi Adamu","doi":"10.54987/jemat.v10i2.774","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.774","url":null,"abstract":"Environmental pollution caused by used engine oil has been on the increase as a result of accidental or deliberate discharge of the oil. Used engine oil is relatively recalcitrant to biodegradation due to the high concentrations of metals from the wear and tear of engine parts. Several physical and chemical methods were employed for the remediation of used engine oil-contaminated soil, but bioremediation remains the most economical especially in remediating polluted soils. Therefore, this work aimed to isolate and screen efficient used engine oil-degrading bacteria from serval automobile workshops located at Gadau town, Itas Gadau, Bauchi state, Nigeria. Soil samples were collected from three different locations and were used to isolate, and screen used engine oil-degrading bacteria. The best degrader was Pseudomonas sp. with 82% degradation of 1% (v/v) of used engine oil after 5 days of aerobic incubation. The result further indicated that degradation occurs best at 1% (v/v) and no degradation was recorded at 3% (v/v) of used engine oil. Pseudomonas sp. can be employed in the field remediation of used engine oil-contaminated soil.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133313997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central Composite Design-based Optimization of Staphylococcus sp. strain Amr-15 Growth on Acrylamide as a Nitrogen Source 葡萄球菌Amr-15以丙烯酰胺为氮源生长的中心复合设计优化

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.735

M. F. Rahman, M. E. Khayat, M. El-Mongy, H. M. Yakasai, N. A. Yasid, M. Shukor

{"title":"Central Composite Design-based Optimization of Staphylococcus sp. strain Amr-15 Growth on Acrylamide as a Nitrogen Source","authors":"M. F. Rahman, M. E. Khayat, M. El-Mongy, H. M. Yakasai, N. A. Yasid, M. Shukor","doi":"10.54987/jemat.v10i2.735","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.735","url":null,"abstract":"As an approach for bioremediation, the decomposition of acrylamide by microorganisms has received gradual but persistent worldwide interest. Prior to this study, a molybdenum-reducing bacteria had been identified and exhibited the ability to breakdown amides. Its key growth parameters on acrylamide were further investigated. A Central Composite Design (CCD) was employed to optimize the two previously identified key factors (incubation time and acrylamide concentration). For the examination of the significant factors or parameters, ANOVA, the perturbation plot, and numerous other diagnostic plots were employed. Using the \"Numerical Optimisation\" toolbox of Design Expert software, predicted ideal conditions were calculated. There were two ideal conditions investigated. The first was to determine the optimal growth under the employed range of variables, while the second was to forecast the optimal growth at the greatest acceptable acrylamide concentration of 1 g/L. The solution for the first predicted model predicted a maximum growth of 8.96 Log CFU/mL (95 percent confidence interval from 8.19 to 9.73), which was confirmed by experimental results with a growth of 9.88 Log CFU/mL (95 percent confidence interval from 9.79 to 9.97), which was close to the predicted values but significantly greater than the predicted values. The second numerical optimization for maximum growth with the highest acrylamide content. The solution had a predicted maximum growth of 7.81 Log CFU/mL (95 percent C.I. from 7.06 to 8.57) and was experimentally confirmed to have a growth of 8.74 Log CFU/mL (95 percent C.I. from 8.56 to 8.92), with the difference not being statistically significant (p0.05) indicating close agreement between predicted and experimental values. The findings of the RSM exercise demonstrated that growth on acrylamide may be optimized more efficiently with RSM than with OFAT, indicating that RSM is more useful in this regard than OFAT.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"193 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131794130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Microplastics Occurrence and Toxic Effects in Marine Environment 海洋环境中微塑料的发生及其毒性效应

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.733

How Swen Yap, M. Shukor, N. A. Yasid

引用次数: 0

Mathematical Modeling of the Inhibition Kinetics of Malachite Green Decolorization by Staphylococcus aureus 金黄色葡萄球菌对孔雀石绿脱色抑制动力学的数学建模

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-12-31 DOI: 10.54987/jemat.v10i2.789

M. Manogaran, Wan Yudreina Yudryk Wan Azni, Nur Muhamad Syahir Abdul Habib, N. A. Yasid

{"title":"Mathematical Modeling of the Inhibition Kinetics of Malachite Green Decolorization by Staphylococcus aureus","authors":"M. Manogaran, Wan Yudreina Yudryk Wan Azni, Nur Muhamad Syahir Abdul Habib, N. A. Yasid","doi":"10.54987/jemat.v10i2.789","DOIUrl":"https://doi.org/10.54987/jemat.v10i2.789","url":null,"abstract":"Basic Green 4 or Malachite Green (MG) is an important dye that found great usage in controlling fish pathogens. The use of MG has been banned but developing, and third-world countries still found applications for this dye. Bioremediation of dyes using microorganisms is on the rise. The ability to accurately predict the rate of bioremediation relies upon the gathering of the accurate rate of decolourisation, which is often inhibited at high concentrations of the toxicant. Various secondary models such as Monod, Haldane, Teissier, Aiba, Yano and Koga, Hans-Levenspiel, Webb and the Luong models were utilized to fit the specific decolourisation rate, and most of them show visually acceptable fitting except Monod and Teissier. The best model based on statistical analysis was Hans-Levenspiel with the highest value for the adjusted coefficient of determination and the lowest values for RMSE, AICc, HQC and BIC and the closest value to 1.0 for accuracy and bias factors. The Hans-Levenspiel model was found to conform to normality tests and is adequate to be used to fit the experimental data. The normality tests carried out using tests such as the Kolmogorov-Smirnov, Wilks-Shapiro and the D'Agostino-Pearson omnibus K2 test shows that the model pass the normality tests with p >0.05 for all normality tests carried out. The experimental data obtained indicates that Malachite Green is toxic and slows down the rate of decolourisation at higher concentrations. The maximum MG specific biodegradation rate (qmax), half-saturation concentration (KS), maximum allowable MG concentration (Sm), and the shape factors (n and m) were 0.136 h-1, 0.56 mg/L, 2691 mg/L, -33.31 and 35.12, respectively. The parameters obtained from this exercise can be utilized to model the bioremediation of MG in the future.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121634221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Atrazine, 2,4-D Amine, Glyphosate and Paraquat Herbicides on Soil Microbial Population 阿特拉津、2,4- d胺、草甘膦和百草枯除草剂对土壤微生物种群的影响

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.693

K. Abdullahi, Khalifa Jamil Saleh, I. Mzungu

{"title":"Effect of Atrazine, 2,4-D Amine, Glyphosate and Paraquat Herbicides on Soil Microbial Population","authors":"K. Abdullahi, Khalifa Jamil Saleh, I. Mzungu","doi":"10.54987/jemat.v10i1.693","DOIUrl":"https://doi.org/10.54987/jemat.v10i1.693","url":null,"abstract":"Herbicides’ toxic impact on the non-target soil microorganisms which play roles in degrading organic matter, nitrogen and nutrient recycling and decomposition needs to be considered. In the present study, the effect of four (4) most commonly used herbicides, viz; atrazine, 2, 4- D amine, glyphosate and paraquat on soil microorganisms was assessed over a period of fifteen continuous days (exposure period). The herbicide treatments were the normal recommended field rate, (6.67 mg active ingredient per gram of soil for atrazine, 6.17 mg for 2, 4-D amine, 5.56 mg for glyphosate, and 2.46 mg for paraquat), half and double of the recommended field rates. Tables 4.1 through 4.5 showed the various heterotrophic bacterial colony counts obtained from the various treatments, ranging from the control (Day 1; no herbicide applied) to days 3, 6, 9 and 15 after the application of the various herbicides, respectively. Two general trends are readily observed: the first is that, upon application of the herbicide, the microbial growth steadily diminishes, up to the 6th day. However, from there it continues to increase till the end of the experiments (day 15). This applies to each herbicide. Firstly, the effect of the addition of the various herbicides was evaluated using two samples, a two-tailed Z-Test for means, at a 95% confidence interval, and the results showed that there is a difference in the bacterial counts before the application of the herbicides (Day 0) and immediately after (day 3), and the difference is statistically significant (Z = 3.32, Z critical = 1.96, P = 0.00090). This indicates that the application of the herbicides affects the bacterial population.","PeriodicalId":393012,"journal":{"name":"Journal of Environmental Microbiology and Toxicology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128473872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIC and NIC Values Determination of Allivum sativum Extract Against Aeromonas hydrophila Using the Lambert-Pearson Model 用Lambert-Pearson模型测定葱提取物对嗜水气单胞菌的MIC和NIC值

Journal of Environmental Microbiology and Toxicology Pub Date : 2022-07-31 DOI: 10.54987/jemat.v10i1.669

Ahmad Syazwan Ismail, M. Shukor, N. A. Masdor

引用次数: 0