尖孢镰刀菌VSTPDK产碱性纤维素酶培养条件的优化

A. Yakubu, G. Uba, A. Vyas
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引用次数: 8

摘要

从印度旁遮普Kapurthala地区的土壤中分离和筛选了产碱性纤维素酶的尖孢镰刀菌VSTPDK。在不同的pH(6、7、8、9、10和11)、温度(30、40、50和60℃)、培养时间(第4、6、8、10和12天)和氮源(NH4SO4、NH4Cl、NaNO3和NH4HCO3)条件下,该菌产生内切葡聚糖酶(CMCase)和外切葡聚糖酶(FPase)。以羧甲基纤维素(CMC)和纤维素粉为唯一碳源。本研究采用响应面法(Response Surface Methodology, RSM)对纤维素酶进行优化,通过一次一因子(OFAT)方法选择三个重要参数。在pH为8、温度为30℃、硫酸铵浓度为1.0g/L的条件下,RSM在pH为8.5、温度为45、硫酸铵浓度为3%的条件下,孵育8 d, CMCsase和FPase的最佳产量分别为3.52U/mL和4.07U/mL;RSM优化培养条件后,CMCase和FPase分别提高了0.39U/mL和0.19U/mL。近十年来,RSM在优化各种理化参数和营养因子方面的应用得到了广泛的关注。在不同行业的应用,可以找到选择不同影响纤维素酶活性因素的方法。这种真菌可以在高达10和50℃的pH下产生相当数量的纤维素酶。据我们所知,这是来自印度旁遮普的嗜碱嗜热尖孢菌VSTPDK的首次报道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of Culture Conditions for the Production of Alkaline Cellulase Enzyme Produced from Fusarium oxysporum VSTPDK
Alkaline cellulase producing Fusarium oxysporum VSTPDK was isolated and screened from the soil of Kapurthala district Punjab, India. This organism produced endoglucanase (CMCase) and exoglucanase (FPase) when grown at a different range of parameters pH (6, 7, 8, 9, 10 and 11), temperature (30, 40, 50 and 60OC), incubation time (4th 6th 8th 10th and 12th day) and nitrogen source (NH4SO4, NH4Cl, NaNO3 and NH4HCO3). Carboxymethylcellulose (CMC) and cellulose powder were used as the sole carbon source. In this research, statistical tools called Response Surface Methodology (RSM) was used for the optimization of cellulase enzyme by selecting three important parameters after one factor at a time (OFAT) approach. Using OFAT, optimum production of both CMCsase (3.52U/mL) and FPase (4.07U/mL) were achieved after 8 days incubation at pH 8, temperature 30OC and 1.0g/L ammonium sulphate while RSM produced CMCase 3.91U/mL and FPase 4.26U/mL respectively when incubated for 8 days at pH 8.5, temperature 45 and 3% ammonium sulphate concentration. Optimization of the culture conditions using RSM leads to an increase of 0.39U/mL (CMCase) and 0.19U/mL (FPase). The use of RSM has gained considerable attention in the past decade in the optimization of various physicochemical parameters and nutritional factors. Its application in different industries may find ways of selecting different factors influencing cellulase activity. The fungus can produce a considerable amount of cellulase enzyme at a pH of up to 10 and 50OC. To our knowledge, this is the first report of alkalothermophilic oxysporum VSTPDK from Punjab, India.
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