African Journal of Infectious Diseases最新文献_第6页

BIOFILM: FORMATION AND NATURAL PRODUCTS' APPROACH TO CONTROL - A REVIEW. 生物膜的形成及天然产物的控制方法综述。

African Journal of Infectious Diseases Pub Date : 2022-08-17 eCollection Date: 2022-01-01 DOI: 10.21010/Ajid.v16i2S.7

Osungunna Michael Oluwole

{"title":"BIOFILM: FORMATION AND NATURAL PRODUCTS' APPROACH TO CONTROL - A REVIEW.","authors":"Osungunna Michael Oluwole","doi":"10.21010/Ajid.v16i2S.7","DOIUrl":"https://doi.org/10.21010/Ajid.v16i2S.7","url":null,"abstract":"Biofilm formation, especially on indwelling medical devices such as catheters, can result in infections and substantially affect patients' quality of life. Biofilm-associated infections have led to increased morbidity and mortality, increased cost of treatment, and length of hospital stay. However, all of the identified consequences of the biofilm-associated infections had been attributed to the reduced susceptibility of biofilm to conventional antimicrobial agents which has necessitated the development of a new strategy for biofilm infections control, thereby making a search for more effective antimicrobial agents from plant source inevitable. So far, some antimicrobial agents (crude or isolated compounds) from plant sources affect a specific stage of biofilm development while a few of them have been developed into a suitable dosage form for biofilm control. In this review, an attempt is made to look into some definitions of biofilm by \"biofilmologists\", stages in biofilm formation, mechanisms of resistance in biofilm, biofilm control strategies, the use of some natural products in biofilm control and concepts of probiotics as agents of biofilm control.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 2 Suppl","pages":"59-71"},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40370788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HOW MISUSE OF ANTIMICROBIAL AGENTS IS EXACERBATING THE CHALLENGES FACING SOMALIA’S PUBLIC HEALTH 滥用抗菌药物如何加剧了索马里公共卫生面临的挑战

African Journal of Infectious Diseases Pub Date : 2022-08-17 DOI: 10.21010/ajidv16i2s.4

Ayan Aden Moussa, B. Garba

引用次数: 3

HOW MISUSE OF ANTIMICROBIAL AGENTS IS EXACERBATING THE CHALLENGES FACING SOMALIA'S PUBLIC HEALTH. 滥用抗菌剂如何加剧了索马里公共卫生面临的挑战。

African Journal of Infectious Diseases Pub Date : 2022-08-17 eCollection Date: 2022-01-01 DOI: 10.21010/Ajid.v16i2S.4

Moussa Ayan Aden, Garba Bashiru

{"title":"HOW MISUSE OF ANTIMICROBIAL AGENTS IS EXACERBATING THE CHALLENGES FACING SOMALIA'S PUBLIC HEALTH.","authors":"Moussa Ayan Aden, Garba Bashiru","doi":"10.21010/Ajid.v16i2S.4","DOIUrl":"https://doi.org/10.21010/Ajid.v16i2S.4","url":null,"abstract":"Background: In contrast to most developed countries, antimicrobial resistance (AMR) has continued to be a serious challenge to public health in the majority of resource-limited countries in Africa.Materials and method: A comprehensive review of all available literature reporting on antimicrobial resistance patterns, antimicrobial drug usage in both human and animals, as well as national AMR regulations in Somalia was undertaken.Results: The review observed that successful AMR control and surveillance among resource-poor nations are affected by a lack of infrastructural and institutional capacities, poor investment in human and material resources, as well as non-adherence to available policies. The humanitarian crisis affecting Somalia has persisted for too long, leading to loss of lives, productivity and dilapidation of public health infrastructures. Somalia like most countries has adopted the One Health approach in developing their soon-to-be gazetted National Action Plan on AMR, which covers both human health, animal health and the environment. Although there are many other similar policy documents and guidelines regulating the usage and administration of antimicrobials in the country, evidence of the implementation indicates there is still a need for more effort.Conclusion: AMR constitute a significant public health problem in Somali, and there is urgent need for gazetting and enforcement of the newly developed national policy. In addition, there is also the need for collaboration with the major stakeholders to develop workable solutions to combat the hazards posed by AMR in the country.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 2 Suppl","pages":"26-32"},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40371245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GENETIC ANALYSIS 21 SHORT TANDEM REPEATS (STR) LOCUS IN MINANGKABAU POPULATION, WEST SUMATERA, INDONESIA 印度尼西亚西苏门答腊岛米南卡保岛21个短串联重复序列（STR）基因座的遗传分析

African Journal of Infectious Diseases Pub Date : 2022-05-06 DOI: 10.21010/ajid.v16i2.4

Citra Manela, R. Susanti, D. Tjong, A. Yudianto

{"title":"GENETIC ANALYSIS 21 SHORT TANDEM REPEATS (STR) LOCUS IN MINANGKABAU POPULATION, WEST SUMATERA, INDONESIA","authors":"Citra Manela, R. Susanti, D. Tjong, A. Yudianto","doi":"10.21010/ajid.v16i2.4","DOIUrl":"https://doi.org/10.21010/ajid.v16i2.4","url":null,"abstract":"Background: Minangkabau is the majority ethnic group in West Sumatra, Indonesia. West Sumatra is a disaster area, especially earthquakes and the potential for a tsunami. Allele frequency for 21 short tandem repeat locus and genetic variation are not well known. This data is essential for calculating the Paternity Index and Match Probability for forensic identification. Materials and methods: This was an observational study. We analyze the GlobalFiller STR loci in 25 unrelated individuals from Minangkabau ethnic group. The DNA was extracted using a Prefiller kit and amplified with a Global Filler kit by a GeneAmp PCR System, followed by capillary electrophoresis using ABI Prism 3500 Genetic Analyzer. Data analysis was performed by using Easy DNA and FORSTAT software. Results: We observed 162 alleles with allele frequencies between 0.02 – 0.36. The highest expected heterozygosity and the highest power of discrimination were at the SE33 loci, and the highest match probability was at the D2S441 locus. The Chi-square test showed that all STR loci followed Hardy–Weinberg equilibrium (p > 0.05). All loci were highly polymorphic (PIC > 0.5). The combined discrimination capacity of each locus in the population was 99,999%. Conclusion: The 21 STR loci are useful for forensic analysis and population genetic studies of the Minangkabau population.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 1","pages":"35 - 41"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46436016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ANTIMICROBIAL RESISTANCE AND MORTALITY. 抗菌药耐药性和死亡率。

African Journal of Infectious Diseases Pub Date : 2022-05-06 eCollection Date: 2022-01-01 DOI: 10.21010/Ajid.v16i2.2

Hori Hariyanto, Corry Quando Yahya, Cucunawangsih Cucunawangsih, Cecilia Lenny Pravita Pertiwi

{"title":"ANTIMICROBIAL RESISTANCE AND MORTALITY.","authors":"Hori Hariyanto, Corry Quando Yahya, Cucunawangsih Cucunawangsih, Cecilia Lenny Pravita Pertiwi","doi":"10.21010/Ajid.v16i2.2","DOIUrl":"10.21010/Ajid.v16i2.2","url":null,"abstract":"Background: Antibiotic resistance has been a long-debated topic since decades ago. The development of stronger, newer antibiotics, implementation of antibiotic stewardship and revised guidelines remain the main focus of our society to prevent resistancy. But is it really resistancy that cause higher mortality to patients with multidrug resistance (MDR) infections?Methods: We conducted a cohort retrospective study from 2016 to 2019 in our Intensive care unit (ICU). Antimicrobial susceptibility test (AST) results were analyzed for their association with patient mortality outcomes.Results: Over the four-year period, 381 positive bacterial cultures were analyzed and 51% of them grew MDR pathogens upon their first culture. The overall mortality rate was 19% (38/195), and there was no significant association between MDR and mortality; p 0.387. A strong association was however found between patients with medical cases with an OR 1.76; CI 1.76-2.55; p 0.003 and those with APACHE scores ≥20 upon admittance to the ICU, OR 1.32; CI 1.68-8.29; p 0.001.Conclusion: Resistancy is not the true cause of mortality. Infection by resistant microbes does not necessarily mean the worst outcome since virulency is the actual cause of pathogenicity, and thus mortality.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 2","pages":"13-20"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10252844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KNOWLEDGE AND COMPLIANCE TO PRACTICE OF PREVENTIVE MEASURES TO COVID-19 AMONG NURSES IN A SELECTED TERTIARY HOSPITAL IN SOUTH-SOUTH, NIGERIA. 尼日利亚南部一所选定的三级医院护士对科维德-19 病毒预防措施的了解和遵守情况。

African Journal of Infectious Diseases Pub Date : 2022-05-06 eCollection Date: 2022-01-01 DOI: 10.21010/Ajid.v16i2.6

Odikpo Linda C, Ezike Okwudili Calistus, Onyia Evert O, Egbuniwe Michael Chiedu, Clementina I Ilo, Obidife I Helen, Clementina U Nwankwo

{"title":"KNOWLEDGE AND COMPLIANCE TO PRACTICE OF PREVENTIVE MEASURES TO COVID-19 AMONG NURSES IN A SELECTED TERTIARY HOSPITAL IN SOUTH-SOUTH, NIGERIA.","authors":"Odikpo Linda C, Ezike Okwudili Calistus, Onyia Evert O, Egbuniwe Michael Chiedu, Clementina I Ilo, Obidife I Helen, Clementina U Nwankwo","doi":"10.21010/Ajid.v16i2.6","DOIUrl":"https://doi.org/10.21010/Ajid.v16i2.6","url":null,"abstract":"Background: Healthcare providers have been at the Front line the response to the COVID-19 disease. Many of them have contracted the disease, and some of them already dead. This study assessed the knowledge, compliance with preventive measures and determined the relationship between knowledge and practice of preventive strategies to COVID-19 among nurses working in a selected hospital in South-South Nigeria.Materials and methods: A cross-sectional descriptive design guided the study. Census method guided the recruitment of all the 378 nurses in the hospital who met the study's inclusion criteria.Results: Majority (360 [95.2%]) of the nurses had good knowledge of the preventive measures to COVID-19 and 311 (82.4%) of the nurses adhere strictly to practice of the preventive strategies to COVID-19. Educational level and years of experience are determinants of knowledge about preventive measures to COVID-19 (p<0.05 respectively) while knowledge, sex, level of education, years of experience, and unit of practice are determinants of compliance to preventive measures to COVID-19 among the nurses (p<0.001). Female nurses (p=0.012), RN/RM qualified nurses (p=0.037), nurses with more than five years of experience, and those in children ward (p=0.020) and maternity complex (p=0.003) significantly comply more to the preventive measures for COVID-19 as shown by their adjusted odds ratios.Conclusion: As knowledge of COVID-19 preventive strategies continues to increase among health workers, there is a need to translate this knowledge into adequate practice in order to minimise the hazardous effect of the pandemic on the health workers especially nurses.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 2","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RHAMNETIN IS A BETTER INHIBITOR OF SARS-COV-2 2’-O-METHYLTRANSFERASE THAN DOLUTEGRAVIR: A COMPUTATIONAL PREDICTION 鼠李素是sars-cov-2 2 ' - o -甲基转移酶的更好抑制剂:计算预测

African Journal of Infectious Diseases Pub Date : 2022-05-06 DOI: 10.21010/Ajid.v16i2.9

A. Rowaiye, A. Oli, O. Onuh, N. W. Emeter, D. Bur, O. A. Obideyi, Oluyomi Cornelius Dayisi, J. N. Akpa, Lovelyn Birah, Edward E. Omaka¹, Frances Otibhor Iseghohi¹, A. P. Otitoju, P. Uzor, J. Okoyeh

{"title":"RHAMNETIN IS A BETTER INHIBITOR OF SARS-COV-2 2’-O-METHYLTRANSFERASE THAN DOLUTEGRAVIR: A COMPUTATIONAL PREDICTION","authors":"A. Rowaiye, A. Oli, O. Onuh, N. W. Emeter, D. Bur, O. A. Obideyi, Oluyomi Cornelius Dayisi, J. N. Akpa, Lovelyn Birah, Edward E. Omaka¹, Frances Otibhor Iseghohi¹, A. P. Otitoju, P. Uzor, J. Okoyeh","doi":"10.21010/Ajid.v16i2.9","DOIUrl":"https://doi.org/10.21010/Ajid.v16i2.9","url":null,"abstract":"Background: The 2’-O-methyltransferase is responsible for the capping of SARS-CoV-2 mRNA and consequently the evasion of the host’s immune system. This study aims at identifying prospective natural inhibitors of the active site of SARS-CoV-2 2’O-methyltransferase (2’-OMT) through an in silico approach. Materials and methods: The target was docked against a library of natural compounds obtained from edible African plants using PyRx - virtual screening software. The antiviral agent, Dolutegravir which has a binding affinity score of -8.5 kcal mol−1 with the SARS-CoV-2 2’-OMT was used as a standard. Compounds were screened for bioavailability through the SWISSADME web server using their molecular descriptors. Screenings for pharmacokinetic properties and bioactivity were performed with PKCSM and Molinspiration web servers respectively. The PLIP and Fpocket webservers were used for the binding site analyses. The Galaxy webserver was used for simulating the time-resolved motions of the apo and holo forms of the target while the MDWeb web server was used for the analyses of the trajectory data. Results: The Root-Mean-Square-Deviation (RMSD) induced by Rhamnetin is 1.656A0 compared to Dolutegravir (1.579A0). The average B-factor induced by Rhamnetin is 113.75 while for Dolutegravir is 78.87; the Root-Mean-Square-Fluctuation (RMSF) for Rhamnetin is 0.75 and for Dolutegravir is 0.67. Also, at the active site, Rhamnetin also has a binding affinity score of -9.5 kcal mol−1 and forms 7 hydrogen bonds compared to Dolutegravir which has -8.5 kcal mol−1 and forms 4 hydrogen bonds respectively. Conclusion: Rhamnetin showed better inhibitory activity at the target’s active site than Dolutegravir.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 1","pages":"80 - 96"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43610001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

KNOWLEDGE, ATTITUDE AND RISK PERCEPTION OF COVID-19 AMONG NIGERIANS 尼日利亚人对新冠肺炎的认识、态度和风险认知

African Journal of Infectious Diseases Pub Date : 2022-05-06 DOI: 10.21010/Ajid.v16i2.7

F. Abayomi, Adeoti Adekunle Olatayo, Adeboye Muhammed Akanbi Nurudeen, Awosanya Joseph Abioye, Oluwadiya Ibironke Omowumi, O. K. Sunday

{"title":"KNOWLEDGE, ATTITUDE AND RISK PERCEPTION OF COVID-19 AMONG NIGERIANS","authors":"F. Abayomi, Adeoti Adekunle Olatayo, Adeboye Muhammed Akanbi Nurudeen, Awosanya Joseph Abioye, Oluwadiya Ibironke Omowumi, O. K. Sunday","doi":"10.21010/Ajid.v16i2.7","DOIUrl":"https://doi.org/10.21010/Ajid.v16i2.7","url":null,"abstract":"Background: COVID-19 emerged as a novel disease with global health importance. Personal and collective behaviours have been modified to prevent the spread of this pandemic. This study is aimed to assess the knowledge, attitude and risk perceptions of Nigerians towards COVID-19. Method: A cross-sectional survey was conducted between the 30th of April and 17th of May 2020 with a national representative among Nigerians using a combination of online and interviewer administered questionnaire. Results: A total of 1,135 respondents participated in the study with a slight male preponderance (M: F=1.5:1). The mean age of the participants was 42±12.2 years with the majority (77%) between the ages of 26 and 55 years. The majority were aware of the pandemic, mostly through mass and social media. Most of the respondents demonstrated good knowledge of COVID-19 but the unaware minority (0.6%) were likewise uneducated. Educational status had no significant association with attitude towards hand washing and wearing of face mask (p>0.05). Risk perception was however low to moderate despite their concerns for COVID-19 and lack of trust in the governments’ response to the disease. Conclusion: The risk perception of COVID-19 among the respondents is not encouraging, hence more advocacy and public orientation must be done to curb further spread of COVID-19 in our setting.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 1","pages":"63 - 70"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43427723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

LOCAL KNOWLEDGE OF ADAPTIVE STRATEGIES AGAINST MALARIA ENDEMICITY IN THE OKAVANGO DELTA, BOTSWANA. 博茨瓦纳奥卡万戈三角洲针对疟疾流行的适应性策略的当地知识

African Journal of Infectious Diseases Pub Date : 2022-05-06 eCollection Date: 2022-01-01 DOI: 10.21010/Ajid.v16i2.3

Maphane Dirontsho, Ngwenya Barbara Ntombi, Kolawole Oluwatoyin Dare, Motsholapheko Moseki Ronald, Pagiwa Vincent

{"title":"LOCAL KNOWLEDGE OF ADAPTIVE STRATEGIES AGAINST MALARIA ENDEMICITY IN THE OKAVANGO DELTA, BOTSWANA.","authors":"Maphane Dirontsho, Ngwenya Barbara Ntombi, Kolawole Oluwatoyin Dare, Motsholapheko Moseki Ronald, Pagiwa Vincent","doi":"10.21010/Ajid.v16i2.3","DOIUrl":"10.21010/Ajid.v16i2.3","url":null,"abstract":"Background: An increasing recognition of the need to eliminate malaria infection globally and particularly in Africa where more than 90% of the cases originate, should enhance community awareness and participation in the same. The perennial freshwater of Okavango Delta located in northern Botswana, which is a source of rural livelihoods and a suitable habitat for the malaria-causing mosquito, and where malaria is endemic provides a suitable environment for the study. Therefore, local households' adaptive strategies against malaria transmission in the Okavango Delta were investigated.Materials and methods: The data used in this paper is a subset or retrospective cohort of 79 households that reported malaria cases/incidences during the first community level household survey conducted from October-November 2015 on 355 households in Shakawe and Ngarange. The households were selected through stratified random sampling in the two study villages. The retrospective cohort household survey was conducted from 7-19 August 2016, in the two study sites. Data were collected through socio-economic survey, key informants' interviews and focus group discussions.Results: The results obtained indicate that most cohort households embraced several adaptive strategies against malaria transmission. These included, inter alia, knowledge capacity building through attendance of health information and education workshops (69%), modifications of houses structures (49.4%), timing of activities and restricting movement at certain times of the day (43%).Discussion: Although most of the stated adaptive strategies such as house screening were not exclusively aimed towards malaria prevention and adaptation, they nonetheless had postive spill over effect that could be a catalyst for eliminating malaria in the study area.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 1","pages":"21-34"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44441566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IMMUNE RESPONSE UPON THE ADMINISTRATION OF RECOMBINANT PROTEIN ANTIBODIES Ag-38 KDa Mycobacterium tuberculosis AND RIFAMPICIN EX-VIVO. 体外给予重组蛋白抗体ag - 38kda结核分枝杆菌和利福平后的免疫反应。

African Journal of Infectious Diseases Pub Date : 2022-01-01 DOI: 10.21010/Ajid.v16i2.8

Tri Yudani Mardining Raras, Almira Fahrinda, Yuliati, Dwi Yuni Nurhidayati, Hidayat Sujuti, Sumarno Reto Prawiro

{"title":"IMMUNE RESPONSE UPON THE ADMINISTRATION OF RECOMBINANT PROTEIN ANTIBODIES Ag-38 KDa Mycobacterium tuberculosis AND RIFAMPICIN EX-VIVO.","authors":"Tri Yudani Mardining Raras, Almira Fahrinda, Yuliati, Dwi Yuni Nurhidayati, Hidayat Sujuti, Sumarno Reto Prawiro","doi":"10.21010/Ajid.v16i2.8","DOIUrl":"https://doi.org/10.21010/Ajid.v16i2.8","url":null,"abstract":"Background: Development a granuloma model resembling latent tuberculosis in vitro is needed with a fast and efficient time to be used as an effective therapy. This study aimed to form efficient granulomas, increase cellular immunity and humoral immunity, and evaluate growth on media using recombinant protein antibody Ag38kDa, Rifampicin, and a combination of both. Peripheral Blood Mononuclear Cell (PBMC) in vitro is derived from a healthy individual separated from monocytes and lymphocytes.Materials and methods: Monocytes are matured into macrophages and then combined macrophages and lymphocytes to the Roswell Park Memorial Institute (RPMI) medium. Flow cytometry analysis was used to count the number of cells, and cytokine levels were measured using ELISA. The result from the treatment was planted on the Lowenstein-Jensen medium.Results: Granulomas-like aggregates was formed after one-day post-infection with Mycobacterium tuberculosis (M.tb). A significant increase in immune response occurred in the number of macrophages, Th1, and Tregs in the combination group compared to the Mtb infection group. The number of Th2 and Th17 cells in the combination group was compared with the control but not significantly. TNF-α cytokine levels increased in the combination group compared to Mtb infection, while in IL-4, we found between all groups, there was no significant difference. Bacterial colonies on culture in the Lowenstein-Jensen medium were only seen in positive controls.Conclusion: Our study concluded that administration of a combination between Ag38kDa recombinant antibody and rifampicin could inhibit granuloma formation and enhance immune response.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 2","pages":"71-79"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10252838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0