中国血吸虫病防治杂志最新文献

{"title":"[Biological threats to global malaria elimination II Deletion in the malaria rapid diagnostic test target <i>Plasmodium falciparum</i> histidine-rich protein 2/3 genes].","authors":"S Xu, J Tang","doi":"10.16250/j.32.1374.2024089","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024089","url":null,"abstract":"<p><p>The global malaria epidemic is still severe. Because of simple procedures, rapid detection and accuracy results, rapid diagnostic test (RDT) has become the most important and the most widely used diagnostic tool for malaria prevention and control. However, deletions in the RDT target <i>Plasmodium falciparum</i> histidine-rich protein 2/3 (<i>Pfhrp2/3</i>) genes may cause false-negative results of RDT, which has been included as one of the four biological threats to global malaria elimination. This article reviews the applications of RDT in the global malaria diagnosis, analyzes the threats and challenges caused by <i>Pfhrp2/3</i> gene deletion, proposes methods for monitoring <i>Pfhrp2/3</i> gene deletion, and summarizes the causes and countermeasures of negative RDT detections, so as to provide insights into consolidation of malaria elimination achievements in China and contributions to global malaria elimination.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"239-242"},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Biological threats to global malaria elimination I Antimalarial drug resistance].","authors":"F Lu","doi":"10.16250/j.32.1374.2024091","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024091","url":null,"abstract":"<p><p>Malaria is an infectious disease that seriously threatens human health. Currently, malaria control mainly depends on antimalarial chemotherapy. However, antimalarial drug resistance is becoming increasingly severe, which poses a great challenge to malaria control, notably treatment of <i>Plasmodium falciparum</i> malaria. To address this challenge, there is a need to facilitate development of novel antimalarial drugs and innovation of treatment strategies, as well as reinforce surveillance and research on antimalarial drug resistance. This article reviews the main categories and use guidelines of current antimalarial agents, summarizes the current status and monitoring methods of antimalarial drug resistance, and proposes the response to antimalarial drug resistance, so as to provide insights into the use of antimalarial drugs and response to antimalarial drug resistance, and contribute to global malaria elimination.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"233-238"},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Epidemiological characteristics of local visceral leishmaniasis in Beijing Municipality from 2021 to 2023].","authors":"W Wu, X Wang, C Kan, D Du, H Liang, D Zhang, Z He","doi":"10.16250/j.32.1374.2023256","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023256","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the epidemiological characteristics of local visceral leishmaniasis in Beijing Municipality from 2021 to 2023, so as to provide insights into formulation of the visceral leishmaniasis control strategy.</p><p><strong>Methods: </strong>Epidemiological data of visceral leishmaniasis cases reported in Beijing Municipality from 2021 to 2023 were collected from the National Health Informatization Disease Prevention and Control Information System, and the epidemiological characteristics of local visceral leishmaniasis cases were analyzed using a descriptive epidemiological method. In November 2021 and 2023, 50 to 100 permanent residents were voluntarily selected within a 100 meter radius of sites where patients lived or acquired <i>Leishmania</i> infections, and venous blood was sampled for anti-<i>Leishmania</i> antibody testing. Venous blood was sampled from dogs for anti-<i>Leishmania</i> antibody testing in natural villages where patients lived or acquired <i>Leishmania</i> infections, or in districts where <i>Leishmania</i> infected dogs were reported. In addition, sandflies were captured with CO₂ mosquito traps and fine mesh nets in natural villages where patients lived or acquired <i>Leishmania</i> infections from May to September, 2021 and 2023, for sandfly species identification.</p><p><strong>Results: </strong>A total of 4 local visceral leishmaniasis cases were reported in Beijing Municipality from 2021 to 2023, with ages of 2 to 77 years, and acquiring <i>Leishmania</i> infections in Mentougou District (2 cases), Changping District (1 case), and Yanqing District (1 case). The anti-<i>Leishmania</i> antibody testing was all negative in 73 human blood samples and the sero-prevalence of anti-<i>Leishmania</i> antibody was 25.00% in 36 venous blood samples from domestic dogs in 2021, with a total of 4 520 <i>Phlebotomus chinensi</i>s captured. The sero-prevalence of anti-<i>Leishmania</i> antibody was 0.51% in 198 human blood samples and 13.58% in 243 venous blood samples from domestic dogs in 2023, with 16.10%, 25.00%, 17.78% and 3.13% sero-prevalence in dogs sampled from Mentougou District, Changping District, Yanqing District and Haidian District, respectively (<i>P</i> = 0.011), while a total of 1 712 <i>Ph. chinensis</i> were captured, including 1 421 female sandflies (86.54%).</p><p><strong>Conclusions: </strong>The prevalence of local visceral leishmaniasis was low in Beijing Municipality from 2021 to 2023; however, there is a risk of further spread in the epidemic foci. Intensified visceral leishmaniasis surveillance and control is recommended.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 4","pages":"388-392"},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Biological threats to global malaria elimination Ⅲ Vector insecticide resistance].","authors":"G Zhu","doi":"10.16250/j.32.1374.2024088","DOIUrl":"10.16250/j.32.1374.2024088","url":null,"abstract":"<p><p>The insecticide resistance is becoming increasingly severe in malaria vectors and has become one of the most important threats to global malaria elimination. Currently, malaria vectors not only have developed high resistance to conventional insecticides, including organochlorine, organophosphates, carbamates, and pyrethroids, but also have been resistant to recently used neonicotinoids and pyrrole insecticides. This article describes the current status of global insecticide resistance in malaria vectors and global insecticide resistance management strategies, analyzes the possible major challenges in the insecticide resistance management, and proposes the response actions, so as to provide insights into global insecticide resistance management and contributions to global malaria elimination.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"243-246"},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Screening and functional analysis of differentially expressed long non-coding RNA in the liver of mice infected with <i>Schistosoma japonicum</i> during the chronic pathogenic stage].","authors":"Y Li, Q Li, W Lin, T Feng, Z Qin, C Cao, S Li, J Xu","doi":"10.16250/j.32.1374.2024076","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024076","url":null,"abstract":"<p><strong>Objective: </strong>To screen differentially expressed long non-coding RNAs (lncRNAs) in the liver of mice infected with <i>Schistosoma japonicum</i> during the chronic pathogenic stage and identify their functions, so as to provide insights into unravelling the role of lncRNAs in <i>S. japonicum</i> infection-induced liver disorders.</p><p><strong>Methods: </strong>Twenty 6-week-old C57BL/6 mice were randomly divided into two groups, of 10 animals each group. Each mouse in the experimental group was infected with (15 ± 2) <i>S. japonicum</i> cercariae via the abdomen for modeling chronic <i>S. japonicum</i> infection in mice, and distilled water served as controls. All mice were sacrificed 70 days post-infection, and mouse liver specimens were sampled for RNA extraction and library construction. All libraries were sequenced on the Illumina NovaSeq 6000 sequencing platform. Data cleaning was performed using the fastp software, and reference genome alignment and gene expression (FPKM) calculation were performed using the HISAT2 software. Potential lncRNA sequences were predicted using the software CNIC, CPC, Pfam, and PLEK, and potential lncRNAs were screened. Differentially expressed lncRNAs were screened with the DESeq2 software and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to identify biological processes and metabolic pathways involved in target genes of differentially expressed lncRNAs.</p><p><strong>Results: </strong>A total of 333 potential lncRNAs were screened, and 67 were identified as differentially expressed lncRNAs, including 49 up-regulated and 18 down-regulated lncRNAs. A total of 53 target genes were predicted for differentially expressed lncRNAs. GO enrichment analysis showed that these target genes were mainly enriched in biological process and molecular function, among which <i>Sema7a</i>, <i>Arrb1</i>, and <i>Ccl21b</i> genes may be hub target genes for positive regulation of extracellular regulated protein kinase 1 (ERK1) and ERK2 cascades and may participate in the regulation of collagen expression. KEGG enrichment analysis showed that the target genes of differentially expressed lncRNAs were mainly enriched in cytokine-cytokine receptor interaction, viral protein interactions with cytokines and cytokine receptors, chemokine signaling pathway, and nuclear factor kappa-B (NF-κB) signaling pathway.</p><p><strong>Conclusions: </strong>This study identifies differentially expressed lncRNAs and functional enrichment of their target genes in the liver of mice during the chronic pathogenic stage of <i>S. japonicum</i> infection. Up-regulated lncRNAs may affect biological processes of ERK1/2 cascades and chemokine signaling pathways via target genes <i>Sema7a</i>, <i>Arrb1</i>, and <i>Ccl21b</i>, thereby affecting collagen expression and inflammatory signal pathways, ultimately affecting the development of liver disorders.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"137-147"},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Epidemiological characteristics and diagnosis of imported <i>Plasmodium malariae</i> and <i>Plasmodium ovale</i> malaria cases in five provinces of China from 2014 to 2021].","authors":"W Lin, D Wang, L Sun, T Zhang, H Yan, W Ruan, Y Liu, D Wu, S Li, J Xia, H Zhu","doi":"10.16250/j.32.1374.2023227","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023227","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the epidemiological characteristics and diagnosis of imported &lt;i&gt;Plasmodium malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria cases in Anhui Province, Hubei Province, Zhejiang Province, Guangxi Zhuang Autonomous Region and Henan Province from 2014 to 2021, so as to provide insights into malaria control in these five provinces.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;All data pertaining to malaria cases reported in five provinces of China were captured from Chinese Disease Control and Prevention Information System from 2014 to 2021, and the epidemiological characteristics of imported &lt;i&gt;P. malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria cases were analysed using a descriptive epidemiological method. The duration from onset of malaria to initial diagnosis, duration from initial diagnosis to definitive diagnosis, institutions of initial and definitive diagnoses, and proportion of correct malaria diagnosis at initial diagnosis were statistically analyzed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 1 223 imported &lt;i&gt;P. malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria cases were reported in Anhui Province, Hubei Province, Zhejiang Province, Henan Province and Guangxi Zhuang Autonomous Region from 2014 to 2021, there were 158 &lt;i&gt;P. malariae&lt;/i&gt; malaria cases (12.92%) and 1 065 &lt;i&gt;P. ovale&lt;/i&gt; malaria cases (87.08%). Totally 98.53% (1 205/1 223) of the imported malaria cases were from Africa, with Angola (18.99%, 30/158), Nigeria (11.39%,18/158), Cameroon (10.76%, 17/158), Ghana (10.13%, 16/158) and the Democratic Republic of the Congo (10.13%,16/158) as predominant countries where &lt;i&gt;P. malariae&lt;/i&gt; malaria cases were from, and Ghana (23.19%, 247/1 065), Cameroon (14.74%, 157/1 065), Nigeria (9.39%, 100/1 065) and Angola (6.95%, 74/1 065) as predominant countries where &lt;i&gt;P. ovale&lt;/i&gt; malaria cases were from. There were significant differences in the duration from onset of malaria to initial diagnosis (χ&lt;sup&gt;2&lt;/sup&gt; = 27.673, &lt;i&gt;P&lt;/i&gt; = 0.000) and duration from initial diagnosis to definitive diagnosis of &lt;i&gt;P. malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria cases (χ&lt;sup&gt;2&lt;/sup&gt; = 29.808, &lt;i&gt;P&lt;/i&gt; = 0.000), and the proportions of correct initial diagnosis of &lt;i&gt;P. malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria cases were 38.61% (61/158) and 56.53% (602/1 065). There were 74.69% (118/158) of &lt;i&gt;P. malariae&lt;/i&gt; malaria cases with definitive diagnosis in county-, city-, and province-level medical institutions, and 79.25% (844/1 065) of &lt;i&gt;P. ovale&lt;/i&gt; malaria cases with definitive diagnosis in county- and city-level medical institutions and county-level centers for disease control and prevention.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The imported &lt;i&gt;P. malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria cases in Anhui Province, Hubei Province, Zhejiang Province, Henan Province and Guangxi Zhuang Autonomous Region from 2014 to 2021 were mainly returned from Africa and the proportion of correct diagnosis of &lt;i&gt;P. malariae&lt;/i&gt; and &lt;i&gt;P. ovale&lt;/i&gt; malaria was low at initi","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 4","pages":"407-411"},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction of a global health talent training evaluation index system based on bibliometric analysis].","authors":"X Tao, X Ma, S Fei, L Duan, S Lü, J Wu","doi":"10.16250/j.32.1374.2024035","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024035","url":null,"abstract":"<p><strong>Objective: </strong>To identify the current research hotspots of global health training, and construct a global health talent training evaluation index system.</p><p><strong>Methods: </strong>Publications pertaining to global health talent training evaluation were retrieved in China National Knowledge Infrastructure, Wanfang Database, and Web of Science Core Collection from 2003 to 2022, and keywords were extracted from eligible publications for co-occurrence and cluster analyses using the CiteSpace software. Based on keywords clustering results, a global health talent training evaluation index system was constructed using a context, input, process, and product (CIPP) evaluation model as a theoretical framework.</p><p><strong>Results: </strong>A total of 692 Chinese publications and 1 264 English publications were included. Keyword co-occurrence and cluster analyses yielded 10 Chinese and 10 English keyword clusters, and the 10 Chinese keyword clusters included analytic hierarchy process, health diplomacy, personnel structure, crossdiscipline, educational assessment, global health discipline development, training needs, curriculum program, quality evaluation and logistics support, while the English keyword clusters included evidence-based practice, capacity building, global health, quality of life, machine learning, leadership, sub-Saharan Africa, health equity, global health security and global health diplomacy. Based on keyword clustering, a global health talent training evaluation index system was constructed with CIPP as the theoretical framework, which contained 4 primary indicators, 15 secondary indicators and 59 tertiary indicators, and the primary indicators included 4 dimensions of context evaluation, input evaluation, process evaluation and product evaluation.</p><p><strong>Conclusions: </strong>A global health talent training evaluation index system has been constructed, which provides an effective evaluation tool and quantitative evidence for future global health talent training.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"191-197"},"PeriodicalIF":0.0,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of ground cage use on <i>Oncomelania hupensis</i> spread].","authors":"M Zhong, X Lu, S Fang, X Jiang, W Gu, Z Xiang","doi":"10.16250/j.32.1374.2023219","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023219","url":null,"abstract":"<p><strong>Objective: </strong>To examine the effect of ground cage use on <i>Oncomelania hupensis</i> spread, so as to provide insights into precision snail control.</p><p><strong>Methods: </strong>Twenty ground cages that were frequently used to capture rice field eels were purchased, including 11 packaging tape-made cages, 7 plastic cages and 2 nylon rope-made cages. The eel-capturing activity was mimicked, and 20 ground cages were assigned in settings with relatively high (1.00 snail/0.1 m² and higher) and low snail densities (< 1.00 snail/0.1 m²) in Xindai Township, Pinghu City, Zhejiang Province during the period from 15 : 00 to 8 : 00 of the following day on April 13, 26 and 28. The numbers of snails carried by different types of ground cages were compared in settings with different types of snail densities using the rank-sum test.</p><p><strong>Results: </strong>A total of 11 cage-times were assigned in settings with a high snail density, and a total of 77 snails were captured, with a mean number of 7 snails in each cage-time and 2.15 snails in 0.1 m² ground cage. The mean numbers of snails carried by packaging tape-made and plastic cages were 2.47 snails/0.1 m² cage and 0.37 snails/0.1 m² cage, respectively. A total of 24 cage-times were assigned in settings with a low snail density, and a total of 8 snails were captured, with a mean number of 0.33 snails in each cage-time and 0.09 snails in 0.1 m² ground cage. The mean numbers of snails carried by packaging tape-made cages were 0.12 snails/0.1 m² cage; however, no snails were carried by plastic or nylon rope-made cages. The number of snails carried by ground cages was higher in settings with a high snail density than in settings with a low snail density (<i>Z</i> = -4.019, <i>P</i> < 0.01), and the number of snails carried by packaging tape-made cages was higher in settings with a high snail density than in settings with a low snail density (<i>Z</i> = -4.086, <i>P</i> < 0.01). No significant differences were found in the numbers of snails carried by different types of ground cages.</p><p><strong>Conclusions: </strong>The use of ground cage in snail habitats is a contributor to snail spread.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"198-200"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prevalence of <i>Echinococcus</i> infections in small rodents in Yushu City, Qinghai Province in 2023].","authors":"G He, S Wu, X Qin, J Ma, T Yu, C Song, X Mo, X Ma, J Ba, G Zheng, B Jiang, T Tian, S Yang, T Zhang, X Zhou","doi":"10.16250/j.32.1374.2024007","DOIUrl":"https://doi.org/10.16250/j.32.1374.2024007","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prevalence of <i>Echinococcus</i> infections in small rodents around human residential areas in Yushu City, Qinghai Province in 2023, so as to provide insights into precision echinococcosis control.</p><p><strong>Methods: </strong>One or two quadrats, each measuring 50 m × 50 m, were randomly assigned in Shanglaxiu Township and Longbao Township, Yushu City, Qinghai Province on June 2023, respectively, and 300 plate-type mouse traps, each measuring 12.0 cm × 6.5 cm, were assigned in each quadrat. Small rodents were captured during the period between 10 : 00 and 18 : 00 each day for 4 days. Then, all captured small rodents were identified and dissected, and liver specimens with suspected <i>Echinococcus</i> infections were subjected to pathological examinations. The <i>Echinococcus</i> cytochrome c oxidase 1 (<i>cox1</i>) gene was amplified using PCR assay, and the sequence of the amplified product was aligned to that was recorded in the GenBank to characterize the parasite species. In addition, a phylogenetic tree of <i>Echinococcus</i> was generated based on the <i>cox1</i> gene sequence using the neighbor-joining method.</p><p><strong>Results: </strong>A total of 236 small rodents were captured in Shanglaxiu and Longbao townships, Yushu City, including 65 Qinghai voles and 51 plateau pikas in Shanglaxiu Township, and 62 Qinghai voles and 58 plateau pikas in Longbao Township, and there was no significant difference in the constituent ratio of small rodents between the two townships (χ² = 0.294, <i>P</i> > 0.05). Seven plateau pikas and 12 Qinghai voles were suspected to be infected with <i>Echinococcus</i> by dissection, and pathological examinations showed unclear structure of hepatic lobules and disordered hepatocyte arrangement in livers of small rodents suspected of <i>Echinococcus</i> infections. PCR assay identified <i>E. shiquicus</i> DNA in 7 Qinghai voles, which were all captured from Shanglaxiu Township. Phylogenetic analysis showed that the <i>cox1</i> gene sequence of <i>Echinococcus</i> in small rodents was highly homologous to the <i>E. shiquicus cox1</i> gene sequence reported previously.</p><p><strong>Conclusions: </strong>Plateau pika and Qinghai vole were predominant small rodents around human residential areas in Yushu City, Qinghai Province in 2023, and <i>E. shiquicus</i> infection was detected in Qinghai voles.</p>","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 2","pages":"169-173"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Familial aggregation of human hookworm infections in Sichuan Province].","authors":"J Luo, H Tian, Y Liu, X Wu, L Tie, L Zhang, X Deng","doi":"10.16250/j.32.1374.2023166","DOIUrl":"https://doi.org/10.16250/j.32.1374.2023166","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the family aggregation of human hookworm infections in Sichuan Province and to identify its influencing factors, so as to provide insights into management of hookworm infections.</p><p><strong>Methods: </strong>Three to four counties (districts) were sampled from basins, hilly regions and mountainous regions around the basins in Sichuan Province from 2017 to 2022 as fixed survey sites, and 17 to 30 counties (districts) were selected as mobile survey sites. At least 1 000 permanent residents at ages of 3 years and older were sampled from each survey site, and hookworm eggs were detected in human stool samples using the Kato-Katz technique. Subjects with 2 and more family members and at least 2 individuals diagnosed with hookworm infections in the county (district) where they lived were selected, and the familial aggregation of hookworm infections was analyzed using the test of goodness of fit for binomial distribution. In addition, the knowledge and practice of hookworm disease control were investigated among residents in Hejiang County and Wutongqiao District, Leshan City, Sichuan Province in 2021 and 2022, and the difference in the knowledge and practice of hookworm disease control was compared between members with and without familial aggregation of hookworm infections.</p><p><strong>Results: </strong>A total of 66 812 residents from 25 196 households were sampled from main endemic areas of hookworm diseases in Sichuan Province from 2017 to 2022 for detection of hookworm infections, and 4 403 infections were identified (6.59% prevalence). The distribution of human hookworm infections in Sichuan Province did not fit the binomial distribution, and showed family aggregations (χ² = 2 116.759, <i>P</i> < 0.001). Family aggregation of human hookworm infections was found in endemic areas with 1% and higher prevalence of human hookworm infections (χ² = 136.006 to 428.738, all <i>P</i> values < 0.001), and family aggregation of human hookworm infections was identified in different years (χ² = 87.615 to 471.838, all <i>P</i> values < 0.001) and in different terrains of endemic areas (χ² = 8.423 to 1 144.176, all <i>P</i> values < 0.001). The members with hookworm infections had median eggs per gram of 180 (interquartile range, 780) in aggregated families and 72 (102) in non-aggregated families (<i>Z</i> = -2.686, <i>P</i> < 0.05). The proportion of members in families with aggregation of hookworm infections who knew the preventive measures of hookworm disease was significantly lower than in non-aggregated families (24.49% vs. 51.72%; χ² = 10.262, <i>P</i> < 0.05), and the proportion of members in families with aggregation of hookworm infections who often worked barefoot on the ground was significantly higher than in non-aggregated families (30.61% vs. 13.25%; χ² = 6.289, <i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>There is a familial ","PeriodicalId":38874,"journal":{"name":"Chinese Journal of Schistosomiasis Control","volume":"36 3","pages":"294-298"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}