中国血吸虫病防治杂志最新文献

{"title":"[Health education and health promotion during the progress towards schistosomiasis elimination Ⅰ Investigation on health education needs and available resource based on integration of community resources].","authors":"J Zhang, L Li, C Cao, S Xie, J Chen, Y Liu, H Xie, F Hu","doi":"10.16250/j.32.1915.2024117","DOIUrl":"10.16250/j.32.1915.2024117","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the health education needs and available resources in schistosomiasis-endemic areas based on integration of community resources, and to explore the operation pathways for health education and promotion during the stage of schistosomiasis elimination.</p><p><strong>Methods: </strong>A community was selected from Nanji Township, Xinjian District, Nanchang City, Jiangxi Province around the Poyang Lake areas as the study site, and a questionnaire survey on health education needs for schistosomiasis control was conducted among permanent residents at ages of 20 years in the community during the period between June and July, 2022 using face-to-face interviews. In addition, the resources available for the community-based schistosomiasis control health education were investigated among workers in township-level specialized institutions, members of civil society organizations, villagers, teachers and high and primary school students through field observations, field surveys and thematic interviews.</p><p><strong>Results: </strong>A total of 304 individuals participated in the questionnaire survey on community-based health education needs for schistosomiasis control, and 94.41% (287/304) and 93.42% (284/304) of the respondents were most interested in knowing/understanding knowledge of the morphology of schistosomes and knowledge associated with the transmission route of schistosomiasis. The four most popular patterns for disseminating schistosomiasis control knowledge included live broadcasting of schistosomiasis control videos (94.41%, 287/304), printed brochures (89.80%, 273/304), special lectures by doctors (62.50%, 190/304) and physical health education materials for schistosomiasis control (61.51%, 187/304). There were 96.71% (294/304) and 77.63% (236/304) of respondents that accepted the participation of township healthcare center and township government/village committees in dissemination of schistosomiasis control knowledge, and there were 95.72% (291/304) and 80.59% (245/304) of respondents that mainly acquired schistosomiasis control knowledge through village allocation of leaflets and volunteer schistosomiasis control activities, respectively. There was 96.71% (294/304) of respondents participating in the dissemination activities of schistosomiasis control knowledge held by village committees, township healthcare centers, and high and primary schools/kinder-gartens, and 61.25% (177/289) of respondents were willing to participate in volunteer schistosomiasis control activities. A total of 115 individuals participated in the survey of available resources for community-based schistosomiasis control health education, and there were a large number of organizational, human and cultural resources in the community that were useful for health communication services.</p><p><strong>Conclusions: </strong>The abundant human and material resources from rural communities in schistosomiasis-endemic areas may be integ","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"626-630"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Plasma exchange combined with continuous renal replacement therapy for imported severe <i>Plasmodium falciparum</i> malaria: a case report].","authors":"X Ma, B Li, X Yu, L Song, L Cheng","doi":"10.16250/j.32.1915.2024130","DOIUrl":"10.16250/j.32.1915.2024130","url":null,"abstract":"<p><p>The article presents the diagnosis and treatment of an imported case with severe <i>Plasmodium falciparum</i> malaria, and the effect of plasma exchange combined with continuous renal replacement therapy. Severe <i>P. falciparum</i> malaria is characterized by complex clinical symptoms and multiple complications, and plasma exchange combined with continuous renal replacement therapy has a satisfactory therapeutic efficacy for severe <i>P. falciparum</i> malaria.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"664-666"},"PeriodicalIF":0.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molluscicidal effect and costs of spraying pyriclobenzuron with drones against <i>Pomacea canaliculata</i>].","authors":"X Zhao, Y Chen, Y Hu, Y Xu, Y Wang, D Lü, C Wan, Y Sun, L Duan, W Wang, S Huang","doi":"10.16250/j.32.1915.2024120","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024120","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To evaluate the molluscicidal effects and costs of spraying 20% suspension concentrate of pyricloben-zuron sulphate (SCPS) with drones against &lt;i&gt;Pomacea canaliculata&lt;/i&gt; in paddy environments, so as to provide insights into the extensive applications of pyriclobenzuron against &lt;i&gt;P. canaliculata&lt;/i&gt;.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;On July 2022, a paddy field was selected from Nanchang City, Jiangxi Province as the study area, and 72 independent rectangular plots measuring 2 m × 1 m were allocated in the study area, with 1 m interval between each plot, and 20 &lt;i&gt;P. canaliculata&lt;/i&gt; snails gently placed in each plot. The activity of 25% wettable powder of pyriclobenzuron sulphate (WPPS) by manual spraying at doses of 0.50, 1.00, 2.00 g/m&lt;sup&gt;2&lt;/sup&gt; and 4.00 g/m&lt;sup&gt;2&lt;/sup&gt; against &lt;i&gt;P. canaliculata&lt;/i&gt; was tested in 54 plots, and manual spraying of 50% wettable powder of niclosamide ethanolamine salt (WPNES) at a dose of 0.10 g/m&lt;sup&gt;2&lt;/sup&gt; served as a chemical control, while manual spraying of the same volume of clean water served as a blank control, with 9 plots in each group. The activity of SCPS against &lt;i&gt;P. canaliculata&lt;/i&gt; was tested in the remaining 18 plots. Based on the molluscicidal tests of WPPS, the molluscicidal effect of SCPS by manual spraying at doses of 0.20, 0.30, 0.40 g/m&lt;sup&gt;2&lt;/sup&gt; and 0.50 g/m&lt;sup&gt;2&lt;/sup&gt; against &lt;i&gt;P. canaliculata&lt;/i&gt; was evaluated, and manual spraying of WPNES at a dose of 0.10 g/m&lt;sup&gt;2&lt;/sup&gt; served as a chemical control, while manual spraying of the same volume of clean water served as a blank control, with three plots in each group. On July 2023, 14 paddy fields with a mean living &lt;i&gt;P. canaliculata&lt;/i&gt; density of &gt; 5 snails/m&lt;sup&gt;2&lt;/sup&gt; were selected from Yujiang District, Yingtan City, Jiangxi Province for molluscicidal tests. Based on the molluscicidal effect of pyriclobenzuron against &lt;i&gt;P. canaliculata&lt;/i&gt; in plots, the molluscicidal effects of WPPS by manual spraying at doses of 0.25, 0.50 g/m&lt;sup&gt;2&lt;/sup&gt; and 1.00 g/m&lt;sup&gt;2&lt;/sup&gt; and manual applications of WPPS at dose of 0.25, 0.50, 1.00 g/m&lt;sup&gt;2&lt;/sup&gt; and 2.00 g/m&lt;sup&gt;2&lt;/sup&gt; mixed with soil were tested, and manual spraying of 0.10 g/m&lt;sup&gt;2&lt;/sup&gt; WPNES served as a chemical control group, while manual spraying of the same volume of clean water served as a blank control, with one paddy field in each group. Based on the effect of pyriclobenzuron against &lt;i&gt;P. canaliculata&lt;/i&gt; in plots, the activity of SCPS sprayed with drones at doses of 0.25 g/m&lt;sup&gt;2&lt;/sup&gt; and 0.50 g/m&lt;sup&gt;2&lt;/sup&gt; mixed in water at 2 kg/667 m&lt;sup&gt;2&lt;/sup&gt; and 4 kg/667 m&lt;sup&gt;2&lt;/sup&gt; was tested against &lt;i&gt;P. canaliculata&lt;/i&gt;, and spraying of the same volume of clean water with drones served as a blank control. All &lt;i&gt;P. canaliculata&lt;/i&gt; snails were captured 3 days and 7 days following chemical treatment in plots and paddy fields and identified for survival, and the mortality and corrected mortality of &lt;i&gt;P. canaliculata&lt;/i&gt; snails were estimated","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 5","pages":"441-449"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of drones in <i>Oncomelania hupensis</i> snail control with molluscicides].","authors":"Y Huang","doi":"10.16250/j.32.1915.2024188","DOIUrl":"10.16250/j.32.1915.2024188","url":null,"abstract":"<p><p><i>Oncomelania hupensis</i> snail control by chemical treatment is an important approach for control and elimination of snails, and an appropriate apparatus for spraying chemicals is an important support to molluscicidal effects. Nevertheless, the research and evaluation of molluscicidal apparatuses have not been paid high attention in the schistosomiasis control programme for a long period of time. Currently, agricultural plant-protective apparatuses have been widely used for chemical treatment. This comment provides a short overview of the application of drones in <i>O. hupensis</i> snail control by chemical treatment, and proposes suggestions for solving the problems of use of drones in <i>O. hupensis</i> snail control by chemical treatment in China.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 5","pages":"439-440"},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Hepatic T cell subtypes and functional analysis among alveolar echinococcosis patients using single-cell RNA sequencing].","authors":"S Chen, X Wang, W Jia, Q Cai, X Zhang, Q Zhang, H Zheng, L Zhu, B Li, W Wang, X Han","doi":"10.16250/j.32.1915.2024172","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024172","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate T cell subtypes and their functions in liver immune microenvironments among patients with alveolar echinococcosis (AE) using single-cell RNA sequencing (scRNA-seq).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Four AE patients that were admitted to Qinghai Provincial People's Hospital in 2023 for hepatic surgery for the first time were enrolled, and liver specimens were sampled 1 cm (peri-lesion, PL group) and &gt; 5 cm from AE lesions (distal lesion, DL group) among each patient. Finally, a total of eight liver specimens were sampled from four AE patients for scRNA-seq analysis. Genome and transcriptome data of liver specimens were processed using the software Cell Ranger and R package. Differentially expressed genes (DEGs) and their biological functions were analyzed using gene ontology (GO) enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, and the primary intercellular communication patterns and interaction mechanisms were identified among T cell subtypes in liver specimens using the CellChat package. In addition, the developmental stages of T cells were subjected to trajectory analysis with the monocle package to investigate the expression of genes associated with cell growth and tumor transformation, and to predict the developmental trajectories of T cells.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;All four AE patients were female, with a mean age of (25.00 ± 9.06) years, and there were three cases from Jiuzhi County, Golog Tibetan Autonomous Prefecture and one case from Chengduo County, Yushu Tibetan Autonomous Prefecture, Qinghai Province. The viability of single-cell samples from eight liver specimens was 90.41% to 96.33%, and a total of 81 763 cells were analyzed, with 19 cell types annotated. Of these cell types, 13 were immune cells (87.60%), and T cells (33.13%), neutrophils (15.40%), and natural killer cells (11.92%) were the three most common cell types. Re-clustering of 27 752 T cells and proliferative T cells identified 10 distinct T cell subtypes, with CD8&lt;sup&gt;+&lt;/sup&gt; cytotoxic T cells (23.43%), CD8&lt;sup&gt;+&lt;/sup&gt; naive T cells (12.80%), and CD4&lt;sup&gt;+&lt;/sup&gt; effector memory T cells (17.73%) as dominant cell types. The proportions of T helper 2 (Th2) cells (5.19% vs. 3.63%; χ&lt;sup&gt;2&lt;/sup&gt; = 38.35, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) and CD4&lt;sup&gt;+&lt;/sup&gt; effector memory T cells (21.59% vs. 13.67%; χ&lt;sup&gt;2&lt;/sup&gt; = 244.70, &lt;i&gt;P&lt;/i&gt; &lt; 0.01) were significantly higher in liver specimens in the PL group than in the DL group, and the proportion of CD4&lt;sup&gt;+&lt;/sup&gt; helper T cells was significantly lower in the PL group than in the DL group (7.50% vs. 14.75%; χ&lt;sup&gt;2&lt;/sup&gt; = 330.52, &lt;i&gt;P&lt;/i&gt; &lt; 0.01). KEGG pathway analysis revealed that Th2 cells were significantly enriched in cell apoptosis and multiple cancer-associated pathways, and CD4&lt;sup&gt;+&lt;/sup&gt; effector memory T cells were significantly enriched in the regulation of cytokines and chronic inflammation, while CD4&lt;sup&gt;+&lt;/sup&gt; helper T cells were significa","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 5","pages":"481-493"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Spatio-temporal distribution characteristics of <i>Oncomelania hupensis</i> snails spread in Suzhou City of Jiangsu Province from 2016 to 2023].","authors":"Q Shi, L Shen, J Zhou, J Wu","doi":"10.16250/j.32.1915.2024155","DOIUrl":"10.16250/j.32.1915.2024155","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the <i>Oncomelania hupensis</i> snails spread and its spatio-temporal clustering characteristics in Suzhou City, Jiangsu Province from 2016 to 2023, so as to provide insights into precision control of <i>O. hupensis</i> snails in the City.</p><p><strong>Methods: </strong><i>O. hupensis</i> snail surveillance data in Suzhou City from 2016 to 2023 were collected, and the areas of <i>O. hupensis</i> snail spread and areas of emerging and re-emerging snail habitats were retrieved. The spatial distribution characteristics and clustering types and locations of environments with <i>O. hupensis</i> snail spread were investigated using global and local spatial auto correlation analyses with the software ArcGIS 10.7, and the clustering and cluster areas of <i>O. hupensis</i> snail spread were identified in Suzhou City using spatio-temporal scans with the software SaTScan 10.0.2.</p><p><strong>Results: </strong><i>O. hupensis</i> snail spread covered an area of 677 171 m² in Suzhou City from 2016 to 2023, including 376 230 m² emerging snail habitats and 300 941 m² re-emerging snail habitats. Global spatial autocorrelation analysis showed overall clustering of <i>O. hupensis</i> snail spread in Suzhou City from 2016 to 2023 (Moran's <i>I</i> = 0.066, <i>P</i> = 0.007), and there were spatial clustering of areas with <i>O. hupensis</i> snail spread in 2019 (Moran's <i>I</i> = 0.086, <i>P</i> = 0.001) and 2021 (Moran's <i>I</i> = 0.045, <i>P</i> = 0.003). Local spatial autocorrelation analysis showed clusters of <i>O. hupensis</i> snail spread in Suzhou City from 2016 to 2023, with high-high clusters in Guangfu Township and Dongzhu Street, and the high-high clusters of <i>O. hupensis</i> snail spread were mainly distributed in southwestern Suzhou City. Spatio-temporal scans identified two clusters of areas with <i>O. hupensis</i> snail spread and areas of re-emerging snail habitats in Suzhou City from 2016 to 2023, with large clustering areas found in Guangfu Township, Dongzhu Street, Tong'an Township and Wangting Township [relative risk (<i>RR</i>) = 22.34, log likelihood ratio (<i>LLR</i>) = 163 295.32, <i>P</i> < 0.001] and small clustering areas in Xukou Township, Mudu Township and Xiangshan Street (<i>RR</i> = 2.73, <i>LLR</i> = 921.92, <i>P</i> < 0.001).</p><p><strong>Conclusions: </strong>There was spatial clustering of <i>O. hupensis</i> snail spread in Suzhou City from 2016 to 2023. Improved quality of <i>O. hupensis</i> snail control and intensified management of environments at a high risk of <i>O. hupensis</i> snail spread are recommended in Suzhou City.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"577-583"},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Impact of patient-centered care services on the treatment compliance among patients with multidrug-resistant or rifampicin-resistant pulmonary tuberculosis].","authors":"J Pan, H Zhou, L Qian, L Zhu, Q Liu","doi":"10.16250/j.32.1915.2023164","DOIUrl":"https://doi.org/10.16250/j.32.1915.2023164","url":null,"abstract":"<p><strong>Objective: </strong>To examine the effect of patient-centered care services on compliance to treatment among patients with multidrug-resistant (MDR) or rifampicin-resistant (RR) pulmonary tuberculosis (PTB), so as to provide the scientific evidence for promoting the widespread application of the appropriate nursing process of MDR/RR-PTB patients in the hospital.</p><p><strong>Methods: </strong>The MDR/RR-PTB patients that were definitely diagnosed at the Sixth People's Hospital of Nantong City during the period from January 2017 to October 2020 were enrolled. The patients with confirmed diagnosis of MDR/RR-PTB during the period January 2017 to December 2018 served as controls, who were given routine care in the hospital, and those with confirmed diagnosis of MDR/RR-PTB during the period January 2019 to October 2020 served as the care group, who were given patient-centered personalized care services, including one-to-one consultations, periodic group activities, informatization case management, and personal reminder for return visits. The proportion of inclusion into treatment, loss to follow-up, return visits and sputum examinations were compared between the care and control groups.</p><p><strong>Results: </strong>A total of 104 MDR/RR-PTB patients were included, including 54 cases in the control group and 50 cases in the care group. There was no significant difference in gender and age distribution between the two groups (χ² = 3.013, 1.336, <i>P</i> > 0.05). The proportion of inclusion into treatment was higher in the care group (100.00%, 50/50) than in the control group (87.04%, 47/54) (<i>P</i> = 0.013), and the proportion of loss to follow-up was lower in the care group (0, 0/43) than in the control group (19.05%, 8/42) (<i>P</i> = 0.002). In addition, the overall proportion of return visits was higher in the care group (93.09%, 377/405) than in the control group (83.56%, 371/444) (χ² = 18.345, <i>P</i> < 0.001), and the proportion of sputum examinations was higher in the care group was (83.70%, 339/405) than in the control group (79.28%, 352/444) (χ² = 2.737, <i>P</i> = 0.098).</p><p><strong>Conclusions: </strong>Patient-centered care services facilitate the improvements in the proportion of inclusion into treatment and compliance to treatment and reduction in the proportion of loss to follow-up among MDR/RR-PTB patients, which deserves widespread applications.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 5","pages":"494-501"},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Impact of <i>Toxoplasma gondii</i> type I rhoptry protein 16 on programmed cell death ligand 1 expression and its binding to programmed cell death 1 in lung adenocarcinoma cells].","authors":"G Li, Y Zhou, S Ma, M Tian, T Dang, Z Zhao","doi":"10.16250/j.32.1915.2024162","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024162","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the impact of <i>Toxoplasma gondii</i> type I, II and III rhoptry protein 16 (ROP16) on programmed cell death ligand 1 (PD-L1) expression in lung adenocarcinoma cells, and to examine the effects of <i>T. gondii</i> type I ROP16 protein on the relative PD-L1 expression, the relative PD-L1 distribution on the cell membrane surface, and the binding of programmed cell death 1 (PD-1) to PD-L1 in lung adenocarcinoma cells.</p><p><strong>Methods: </strong>Lentiviral vectors overexpressing <i>T. gondii</i> type I, II and III ROP16 proteins were generated, and transfected into the human lung adenocarcinoma A549 cell line. A549 cells were used as a blank control group, and A549 cells transfected with an empty lentiviral expression vector were used as a negative control group, while A549 cells transfected with lentiviral vectors overexpressing <i>T. gondii</i> type I, II and III ROP16 proteins served as experimental groups. Stably transfected cells were selected with puromycin and verified using Western blotting, quantitative real-time PCR (RT-qPCR), and immunofluorescence assays. The PD-L1 expression was quantified at translational and transcriptional levels using Western blotting and RT-qPCR assays in A549 cells in the five groups, and the relative PD-L1 distribution was detected on the A549 cell membrane surface using flow cytometry. In addition, the effect of <i>T. gondii</i> type I ROP16 protein on the PD-1/PD-L1 binding was measured in A549 cells using enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>The relative ROP16 protein expression was 0, 0, 1.546 ± 0.091, 1.822 ± 0.047 and 2.334 ± 0.089 in the blank control group, negative control group, and the <i>T. gondii</i> type I, II and III ROP16 protein overexpression groups (<i>F</i> = 1 339.00,P < 0.001), and the relative <i>ROP16 mRNA</i> expression was 2.153 ± 0.949, 2.436 ± 1.614, 14.343 ± 0.020, 12.577 ± 0.285 and 15.090 ± 0.420 in the blank control group, negative control group and the <i>T. gondii</i> type I, II and III ROP16 protein overexpression groups, respectively (<i>F</i> = 483.50,P < 0.001). The ROP16 expression was higher in the <i>T. gondii</i> type I, II and III ROP16 protein overexpression groups than in the blank control group at both translational and transcriptional levels (allP values < 0.001). Immunofluorescence assay revealed that <i>T. gondii</i> type I, II and III ROP16 proteins were predominantly localized in A549 cell nuclei. Western blotting showed that the relative PD-L1 protein expression was 0.685 ± 0.109, 0.589 ± 0.114, 1.007 ± 0.117, 0.572 ± 0.151, and 0.426 ± 0.116 in the blank control group, negative control group, and the <i>T. gondii</i> type I, II and III ROP16 protein overexpression groups (<i>F</i> = 9.46,P < 0.05), and RT-qPCR assay quantified that the relative <i>PD-L1 mRNA</i> expression was 1.012 ± 0.190, 1.281 ± 0.465, 1.950 ± 0.175, 0.889 ± 0.251, and 0.230 ± 0.192 in the blank contro","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"37 1","pages":"44-54"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molluscicidal effect of spraying 5% niclosamide ethanolamine salt granules with drones against <i>Oncomelania hupensis</i> in marshland areas].","authors":"C Cao, J Zhang, Y Li, X Shen, J He, Z Bao, S Guo, K Yang, J Xu, S Li, X Zhou","doi":"10.16250/j.32.1915.2024115","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024115","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the molluscicidal effect of spraying 5% niclosamide ethanolamine salt granules with drones against of <i>Oncomelania hupensis</i> snails in snail habitats in marshland areas.</p><p><strong>Methods: </strong>From September to October, 2022, marshlands were sampled from Dantu District, Zhenjiang City, Jiangsu Province as study areas, and assigned into four groups, of approximately 3 000 m² per group. In Group A, environmental cleaning was performed, followed by spraying 5% niclosamide ethanolamine salt granules with knapsack sprayers at a dose of 40 g/m², and in Group B, 5% niclosamide ethanolamine salt granules were sprayed with knapsack sprayers at a dose of 40 g/m² without environmental cleaning, while in Group C, environmental cleaning was conducted, followed by spraying 5% niclosamide ethanolamine salt granules with drones at a dose of 40 g/m², and in Group D, 5% niclosamide ethanolamine salt granules were sprayed with drones at a dose of 40 g/m² without environmental cleaning. Then, the study areas in each group were equally divided into six blocks, with Block 1 for baseline surveys and blocks 2 to 6 for snail surveys 1, 3, 5, 7, 14 days following chemical treatment. The mortality of snails and the reduction of the density of living snails were calculated.</p><p><strong>Results: </strong>A total of 132 frames were surveyed during the period from September to October 2022, and the occurrence of frames with living snails and means density of living snails were 61.36% (81/132) and 1.58 snails/0.1 m², respectively. The overall mortality rates of snails were 43.02% (77/179), 38.69% (77/199), 47.78% (86/180) and 31.02% (58/187) 14 days following chemical treatment in groups A, B, C and D, respectively (χ² = 11.646, <i>P</i> < 0.05), and there were differences detected in the snail mortality between group A and D, and between groups C and D (both <i>P</i>_adjusted values < 0.05). The adjusted mortality rates of snails were 37.42%, 36.07%, 38.85% and 40.40% in groups A, B, C and D 14 days post-treatment, and the density of living snails decreased by 48.10%, 63.29%, 67.09% and 69.62% 14 days post-treatment relative to pre-treatment, respectively.</p><p><strong>Conclusions: </strong>Chemical treatment with drones is feasible for <i>O. hupensis</i> snail control in marshland areas; however, the molluscicidal effect of spraying 5% niclosamide ethanolamine salt granules with drones is comparable to spraying chemicals manually in marshland areas regardless of environmental cleaning.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 5","pages":"531-534"},"PeriodicalIF":0.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Population genetic diversity of <i>Culex tritaeniorhynchus</i> in Jining City of Shandong Province based on the mitochondrial cytochrome C oxidase I gene].","authors":"Y Zhang, C Zang, X Pan, M Gong, H Liu","doi":"10.16250/j.32.1915.2024119","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024119","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To understand the genetic basis of the adaptation of &lt;i&gt;Culex tritaeniorhynchus&lt;/i&gt; to different environmental ecology in Jining City, Shandong Province, so as to provide insights into understanding of the population structure or isolation pattern of &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; in the city.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Seven sampling sites were selected from urban, suburban and rural areas of Jining City, Shandong Province from June to August 2023, and mosquitoes were collected using mosquito-trapping lamps. All collected adult mosquitoes were identified morphologically. Genomic DNA was extracted from a single female &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; mosquito, and the mitochondrial cytochrome C oxidase I (&lt;i&gt;COI&lt;/i&gt;) gene was amplified using a PCR assay, sequenced and subjected to molecular identification. The number of haplotypes, haplotype diversity (&lt;i&gt;Hd&lt;/i&gt;), nucleotide diversity (&lt;i&gt;Pi&lt;/i&gt;), and average number of nucleotide differences (&lt;i&gt;K&lt;/i&gt;) of &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; DNA sequences were estimated among different sampling sites using the software DnaSP 6, and a neutrality test was performed. The fixation index (&lt;i&gt;F&lt;sub&gt;ST&lt;/sub&gt;&lt;/i&gt;), and gene flow (number of migrants, &lt;i&gt;Nm&lt;/i&gt;) of &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; populations were calculated using the software Arlequin 3.5.2, and subjected to analysis of molecular variance (AMOVA). In addition, a haplotype network diagrams and a phylogenetic tree of &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; populations were created using the software PopART and MEGA 11, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 420 sequences were successfully amplified from the &lt;i&gt;COI&lt;/i&gt; gene of &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; samples collected from 7 sampling sites in Jining City, and a gene fragment sequence with a length of 603 bp was obtained, with 55 variable sites and 46 haplotypes and without insertion or deletion mutations. Of the 46 haplotypes, H01 was the dominant shared haplotype, and the haplotype frequency increased gradually from urban areas (34.00%) to rural areas (47.00%). The mean &lt;i&gt;Hd&lt;/i&gt;, &lt;i&gt;Pi&lt;/i&gt; and &lt;i&gt;K&lt;/i&gt; values of &lt;i&gt;Cx. tritaeniorhynchus COI&lt;/i&gt; genes were 0.814, 0.024 and 14.129, 0.489, 0.016 and 7.941 and 0.641, 0.016 and 10.393 in suburban, urban, and rural areas, respectively, with the highest population diversity of &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; in suburban areas and the lowest in urban areas. Paired &lt;i&gt;F&lt;sub&gt;ST&lt;/sub&gt;&lt;/i&gt; analysis among different types of sampling sites showed that the mean &lt;i&gt;F&lt;sub&gt;ST&lt;/sub&gt;&lt;/i&gt; value was 0.029 between urban and suburban areas, indicating more frequent inter-population communication. AMOVA revealed that the percentage of intra-population variation (95.74%) was higher than that of inter-population variation (4.26%). Neutrality tests showed deviation from neutrality in &lt;i&gt;Cx. tritaeniorhynchus&lt;/i&gt; populations collected from Nanyang Township (Tajima's &lt;i&gt;D&lt;/i&gt; = 2.793, Fu's &lt;i&gt;Fs&lt;/i&gt; = 6.429, both &lt;i&gt;P&lt;/i&gt; values &lt; 0.05). In a","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 5","pages":"466-473"},"PeriodicalIF":0.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142773246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}