Tokai Journal of Experimental and Clinical Medicine最新文献_第4页

Metabolism of nitric oxide under the conditions chronic alcohol intoxication modelling 慢性酒精中毒条件下一氧化氮代谢模型

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-31 DOI: 10.35339/ekm.2023.92.1.myk

A. Mykytenko

{"title":"Metabolism of nitric oxide under the conditions chronic alcohol intoxication modelling","authors":"A. Mykytenko","doi":"10.35339/ekm.2023.92.1.myk","DOIUrl":"https://doi.org/10.35339/ekm.2023.92.1.myk","url":null,"abstract":"It was experimentally shown that ethanol affects the production of nitric oxide in rats. However, nitric oxide can have both a protective effect by weakening the harmful effect of ethanol on the microcirculation of the liver, and lead to liver damage by active forms of nitrogen. The purpose of the study is to study changes in the nitric oxide cycle under the conditions of modeling chronic alcohol intoxication in rats. Experiments were performed on 30 white, mature male Wistar rats, weighing 180–220 g. The animals were divided into 2 groups: I – control (n=6); II group – animals with alcoholic hepatitis (n=24) modelled by the method of forced intermittent alcoholization for 5 days, with a repeat after two days by intraperitoneal injection of a 16.5% ethanol solution in a 5% glucose solution, at the rate of 4 ml/kg of body weight. Animals were removed from the experiment on days 10, 14, 21 and 28 by taking blood from the right ventricle of the heart under thiopental anesthesia. The activity of inducible and constitutive isoforms of NO-synthase, concentration of nitrite, nitrosothiols and peroxynitrites of alkali and alkaline earth metals, the activity of nitrite reductase, nitrate reductase and arginase were determined in rat liver homogenate. Chronic alcohol intoxication modelling for 10–28 days leads to a violation of the formation and metabolism of nitric oxide with the predominant formation of its toxic metabolites, such as peroxynitrites and nitrites, which threatens the development of nitrosative stress in the liver. Chronic alcohol intoxication on the 10th–28th days of the experiment is accompanied by a sharp decrease in the activity of the arginase-dependent pathway of arginine metabolism in the liver of rats, which indicates a violation of the deamination processes in the Krebs-Handzeleit cycle.\u0000\u0000Keywords: nitrites, NO-synthase, peroxynitrite, alcohol, liver, rats.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74287223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of the COVID-19 pandemic on pediatric patients with otitis media with effusion COVID-19大流行对儿童中耳炎伴积液的影响

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.21

N. Turgut

{"title":"The effects of the COVID-19 pandemic on pediatric patients with otitis media with effusion","authors":"N. Turgut","doi":"10.52142/omujecm.40.1.21","DOIUrl":"https://doi.org/10.52142/omujecm.40.1.21","url":null,"abstract":"Otitis media with effusion (OME) is a frequently self-limiting middle ear fluid accumulation. During the COVID-19 pandemic, there was a decrease in patient referrals to pediatric Otorhinolaryngology (pENT) outpatient clinics. The aim of this study was to compare the patients who presented at our pENT outpatient clinic with OME during the pandemic with patients who presented during the equivalent period before the pandemic, and to investigate the effects of pandemic measures on OME. The study included patients aged 1-15 years who presented at the pENT Outpatient Clinic due to OME. Four groups were created based on the date of March 2020, when the first COVID-19 case was recorded in Turkey. The groups were formed as one for each year from March 2018 to March 2022. The total number of patients admitted to the pENT outpatient clinic was recorded. Group 1 (March 2018-March 2019) included 1338 patients diagnosed with OME, which constituted 12% of the total number of patients. Group 2 (March 2019- March 2020) included 1238 patients, Group 3 (March 2020-March 2021) 241 (8%), and Group 4 (March 2021-March 2022) 432 (9.4%). From this study, it was observed that the greater attention paid to the mask, social distance and hygiene rules during the COVID-19 pandemic, and the implementation of distance education decreased the frequency of upper respiratory tract infections, and had a positive effect on the number of OME cases.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77455152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell applications in female infertility – A rewiev 干细胞在女性不孕症中的应用综述

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.26

A. Özdemir, Ayşe Şeyma Taştan

引用次数: 0

Correlation of blood glucose and prostatic-related parameters in patients with prostate enlargement 前列腺肿大患者血糖与前列腺相关参数的相关性

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.19

I. Yatindra, Anak Agung Sagung Mirah Prabandari, P. Tirtayasa, I. Yatindra, A. N. O. Diatmika, Kadek Cindy Merni̇ka

{"title":"Correlation of blood glucose and prostatic-related parameters in patients with prostate enlargement","authors":"I. Yatindra, Anak Agung Sagung Mirah Prabandari, P. Tirtayasa, I. Yatindra, A. N. O. Diatmika, Kadek Cindy Merni̇ka","doi":"10.52142/omujecm.40.1.19","DOIUrl":"https://doi.org/10.52142/omujecm.40.1.19","url":null,"abstract":"Enlarged prostate, either in benign prostatic hyperplasia or prostate cancer is the predominant urological disease affecting older men. Metabolic syndrome including high blood glucose was considered to take part in the development of prostate enlargement, although their association is still unclear. This study aims to investigate the correlation between blood glucose with prostatic-related parameters. A cross-sectional study was conducted involving 91 men with prostate enlargement from Urology Polyclinic, Tabanan Hospital, Bali Province, Indonesia. Prostatic-related parameters collected include patient’s international prostate symptoms score using the questionnaire, prostate-specific antigen and random blood glucose analysis from serum blood sample, and prostate volume determined with transabdominal ultrasonography. Patient’s mean age was 65.4 ± 10 years, mean prostate volume 52.7 ± 26 ml, mean prostate-specific antigen (PSA) 9.2 ± 17 ng/ml, mean international prostatic symptoms score (IPSS) 14.31 ± 7.1, mean random blood glucose 121.1 ± 40.5 mg/dl. This study found weak positive correlation between random blood glucose and prostate volume (r=0.269; p=0.01). PSA and prostate volume showed moderate positive correlation (r=0.365; p<0.001). No correlation between random blood glucose with PSA or IPSS was found in this study. This study found random blood glucose has positive correlation with prostate volume. We recommend enhancing blood glucose control to prevent the progress of prostate enlargement.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"198 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77306837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of transvaginal ultrasonography in determining endometrium cancer risk in asymptomatic postmenopausal women with thickened endometrium 经阴道超声在确定无症状的绝经后子宫内膜增厚妇女子宫内膜癌风险中的作用

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.9

Osman Samet Günkaya, A. Tekin, M. Tekelioğlu, Zeynep Gedik Özköse, Ayşegül Bestel, Ü. Kalkan, N. Tuğ

{"title":"Role of transvaginal ultrasonography in determining endometrium cancer risk in asymptomatic postmenopausal women with thickened endometrium","authors":"Osman Samet Günkaya, A. Tekin, M. Tekelioğlu, Zeynep Gedik Özköse, Ayşegül Bestel, Ü. Kalkan, N. Tuğ","doi":"10.52142/omujecm.40.1.9","DOIUrl":"https://doi.org/10.52142/omujecm.40.1.9","url":null,"abstract":"The aim of this study is to determine the cut-off value for the histopathological evaluation of premalignant-malignant endometrial pathologies from benign pathologies in postmenopausal asymptomatic patients with increased endometrial thickness. This cross-sectional study included a population that included asymptomatic 481 postmenopausal women with an endometrial thickness of more than 5mm in TVU who underwent diagnostic/ operative hysteroscopy and full curettage between January 2015 and January 2018. Demographic characteristics TVU, hysteroscopy findings of patients were recorded. As a result, in the histopathological outcome, 154(3%) women were evaluated as having normal endometrium, 189(39.3%) women as having endometrial polyps, 93(19.3%) women as having endometrial atrophy, and 23(4.6%) women as having endometrial simple hyperplasia, 5(1%) women as having endometrial simple hyperplasia, 17(3.5%) women as having endometrial atrophy and only one (0.1%) woman as having fibroids. In the 187 postmenopausal women with normal diagnostic hysteroscopic evaluation, histopathological findings were: 13(7%) endometrial hyperplasia, 2(1.1%) atypical endometrial hyperplasia, 27(14.4%) endometrial polyps, 4 2.1%) endometrial atrophy, and 2(1.1%) endometrial carcinoma. The endometrial thickness was analyzed with the ROC curve for cutoff value differentiating atypical endometrial hypreplasia/endometrial carcinoma from benign lesions and 10.5 mm was found with 90% sensitivity and 63% specificity. In conclusion, hysteroscopy is highly effective for identifying the endometrium and focal intracavitary pathologies such as polyps, myomas and foreign bodies in women with abnormal uterine bleeding. However, for the diagnosis of endometrial hyperplasia and cancer, hysteroscopic-guided biopsy with uterine curettage seems to be the best method.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78148286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association between KRAS gene expression and breast tumors in a sample of Iraqi women 伊拉克妇女样本中KRAS基因表达与乳腺肿瘤的关系

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.5

Roya H. AL-HADDAD, Hameed M. Jasim

引用次数: 0

Epidemiological and clinical characteristics, management, and outcomes of antenatal hydronephrosis: A single-centre experience of 229 cases 流行病学和临床特征，管理和结局产前肾积水:单中心经验229例

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.24

M. Önal, H. G. Önal

{"title":"Epidemiological and clinical characteristics, management, and outcomes of antenatal hydronephrosis: A single-centre experience of 229 cases","authors":"M. Önal, H. G. Önal","doi":"10.52142/omujecm.40.1.24","DOIUrl":"https://doi.org/10.52142/omujecm.40.1.24","url":null,"abstract":"The advances in utilization of USG in antenatal routine follow-up resulted with increased diagnosis of antenatal hydronephrosis (ANH). This study was conducted to elaborate the epidemiological and clinical characteristics, management, outcomes, and possible risk factors of the ANH. A total of 229 cases diagnosed with ANH during the antenatal follow-up at the Obstetrics and Gynecology Department of OndokuzMayis University between 2004 and 2022 were included. The ANH was defined as an USG finding suggesting a hydronephrosis 7 mm. The epidemiological and clinical characteristics, risk factors, treatment and outcomes in the postnatal period were assessed retrospectively. About 75% of the cases were male, 8% were premature births, and the mean gestational week of diagnosis was 22±3 weeks. About 43.7% of mothers had urinary tract infections, and family history of a kidney disease was present in 24.5% of mothers’ and 20.5% of fathers’ family histories.38.7% of cases undergone surgery. At the end of 6-month follow-up, 37.3% had regressed and 38.7% of them had stable hydronephrosis, and 18.9% of them had normal findings in USG. Male gender, increased gestational urinary tract infections, and family histories of parents for a kidney disease were found as possible risk factors for development of ANH. Close follow-up and timely intervention including surgery provides favorable outcomes in these cases.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89515207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What is the effect of vitamin D deficiency on Pap smear test results in the postmenopausal period? 绝经后维生素D缺乏对巴氏涂片检查结果有什么影响?

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.15

Asena Ayar Madenli, İnci Öz, Ergul Demircivi

{"title":"What is the effect of vitamin D deficiency on Pap smear test results in the postmenopausal period?","authors":"Asena Ayar Madenli, İnci Öz, Ergul Demircivi","doi":"10.52142/omujecm.40.1.15","DOIUrl":"https://doi.org/10.52142/omujecm.40.1.15","url":null,"abstract":"This study aimed to determine the impact of vitamin D deficiency on Pap smear test results in women in the postmenopausal period. This cross-sectional study included 394 women who applied to İstanbul Medeniyet University, Göztepe Training and Research Hospital gynecology and obstetric clinic for routine Pap smear tests. This study was conducted between May 2019 and December 2020. Cases were divided into severe deficiency (<12 ng/ml), moderate deficiency (12-20 ng/ml), deficiency (21-30 ng/ml), and sufficiency (>30 ng/ml) according to the vitamin D levels measured in the postmenopausal period within the scope of check-up examinations. Pap smear test results compared with vitamin D levels. The mean age of the study group was 48.70±3.46 years. There was a statistically significant association between vitamin D values and Pap smear test results (p<0.05). Vitamin D greater than 20 ng/ml had a significant relationship with the normal cytology results. There was a statistically significant association between vitamin D lower than 20 ng/ml and cervicitis cytology results. The results showed that the serum vitamin D and the Pap smear test results had a significant association. It is recommended that physicians prescribe the use of vitamin D supplements in women in the high-risk group of cervical cancer.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83678676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective impact of glutamine on anti-tuberculosis drug-induced nephrotoxicity in Wistar rats 谷氨酰胺对Wistar大鼠抗结核药物肾毒性的保护作用

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.2

Elias Adikwu, Martins Mbonu, Tobechi Brendan Nnanna

{"title":"The protective impact of glutamine on anti-tuberculosis drug-induced nephrotoxicity in Wistar rats","authors":"Elias Adikwu, Martins Mbonu, Tobechi Brendan Nnanna","doi":"10.52142/omujecm.40.1.2","DOIUrl":"https://doi.org/10.52142/omujecm.40.1.2","url":null,"abstract":"This study assessed the protective effect of glutamine (GTN) against rifampicin/isoniazid/pyrazinamide/ethambutol (RIPE)-induced nephrotoxicity in rats. Thirty adult Wistar rats (200±20 g) of both sexes were grouped into 6 of 5 rats/group. The rats were treated daily for 30 days as follows: Group 1 (Vehicle control [normal saline 0.2mL]), group 2 (GTN 200 mg/kg), group 3 (RIPE 150, 75, 400 and 275 mg/kg in vehicle), group 4 (GTN 50 mg/kg +RIPE), group 5 (GTN 100 mg/kg +RIPE) and group 6 (GTN 200 mg/kg +RIPE). After treatment, blood samples were obtained and assessed for serum renal biomarkers. Kidneys were harvested, weighed and assessed for oxidative stress markers and histology. RIPE significantly (p<0.01) decreased body weight and significantly (p<0.01) increased kidney weight when compared to the control. Serum urea, creatinine, uric acid levels and kidney malondialdehyde levels were significantly (p<0.001) increased in RIPE-treated rats when compared to the control. Serum total protein, albumin, kidney glutathione, catalase, superoxide dismutase and glutathione peroxidase levels were significantly decreased (p<0.001) in RIPE-treated rats when compared to the control. RIPE caused tubular necrosis and collapsed glomeruli in the kidneys of rats. However, body and liver weights were significantly restored in GTN 100 mg/kg +RIPE and GTN 200 mg/kg +RIPE-treated rats at p<0.05 and p<0.01, respectively when compared to RIPE. Serum and kidney oxidative stress markers were restored in GTN 50 mg/kg +RIPE, GTN 100 mg/kg +RIPE and GTN 200 mg/kg +RIPE-treated rats at p<0.05, p<0.01 and p<0.001 respectively, when compared to RIPE. GTN restored kidney histology. GTN protects against RIPE-induced nephrotoxicity in a dose-related fashion.","PeriodicalId":38819,"journal":{"name":"Tokai Journal of Experimental and Clinical Medicine","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86171729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental intervertebral disc degeneration models 实验性椎间盘退变模型

Tokai Journal of Experimental and Clinical Medicine Pub Date : 2023-03-18 DOI: 10.52142/omujecm.40.1.29

O. Sönmez, Ayşe İkinci Keleş

引用次数: 0