Journal of Vaccines, Immunology and Immunopathology最新文献_第4页

Assessment of the Diagnostic Ability of the DIVA Real-Time PCR in a Duplex Configuration to Differentiate Between the Turkey Meningoencephalitis Vaccine and Wild-Type Viruses DIVA实时聚合酶链反应(Real-Time PCR)对土耳其脑膜脑炎疫苗和野生型病毒的双重诊断能力评估

Journal of Vaccines, Immunology and Immunopathology Pub Date : 2018-07-16 DOI: 10.29011/2575-789x.000132

I. Davidson, Yael Shimshon

{"title":"Assessment of the Diagnostic Ability of the DIVA Real-Time PCR in a Duplex Configuration to Differentiate Between the Turkey Meningoencephalitis Vaccine and Wild-Type Viruses","authors":"I. Davidson, Yael Shimshon","doi":"10.29011/2575-789x.000132","DOIUrl":"https://doi.org/10.29011/2575-789x.000132","url":null,"abstract":"of the Diagnostic Ability of the DIVA Real-Time PCR in a Duplex Configu ration to Differentiate Between the Turkey Vaccine and Wild-Type Viruses. Abstract The avian flavivirus Turkey Meningoencephalitis Virus (ITV) causes a neuroparalytic disease of commercial turkeys, expressed in paresis, incoordination, dropping wings and mortality that is controlled by vaccination. The newly developed ITV real time RT-PCR Differentiating Infected from Vaccinated Animals assay, was further developed and transformed into a one-step duplex assay to distinguish between wild-type ITV strains and vaccine virus and to help identify in one amplification the virus type involved in the recent emergence of ITV in the summer-fall of 2017. The performance of the newly developed duplex DIVA assay was equal to that of the two monoplex assays in detecting clinical cases evaluated on the recent outbreak that affected most of the commercial turkey flocks of the age of ITV clinical affection. Using the ITV vaccine virus, we showed that the amplification parameters of the single-and duplex DIVA real-time PCR were similar. Next, the clinical cases were similarly amplified with the ITV-DIVA assay both as single-and duplex DIVA real-time PCR. In conclusion, a powerfully distinctive, sustainable and sensitive one-step duplex assay was put in action on the 2017 ITV outbreak.","PeriodicalId":386740,"journal":{"name":"Journal of Vaccines, Immunology and Immunopathology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127489870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Control of Foot-and-Mouth Disease When Vaccines are Not Available 在没有疫苗的情况下控制口蹄疫

Journal of Vaccines, Immunology and Immunopathology Pub Date : 2018-07-09 DOI: 10.29011/2575-789x.000131

J. Cummins

{"title":"Control of Foot-and-Mouth Disease When Vaccines are Not Available","authors":"J. Cummins","doi":"10.29011/2575-789x.000131","DOIUrl":"https://doi.org/10.29011/2575-789x.000131","url":null,"abstract":"Vaccination and depopulation are the methods for control of Foot-and-Mouth Disease (FMD). Another tool is needed to manage FMD Virus (FMDV) if sero-type vaccines are unavailable. The USDA plans to vaccinate cattle if there is a major FMDV outbreak in the USA, but this plan is hampered by the lack of a stockpile of FMDV vaccines to treat millions of animals in a timely fashion. In the absence of FMDV specific vaccines strategies to induce or administer interferon (IFN) might limit FMDV replication and disease in cattle and swine. A group of USDA Animal Research Service (ARS) scientists have reported that the FMDV establishes infection in susceptible cells/hosts by its ability to subvert key host defenses, specifically the inducible IFN response. FMDV inhibits production of IFN alpha (α) [l] and blocks a key IFNinducible, antiviral pathway, i.e.Double-Stranded RNA (dsRNA) dependent Protein Kinase R (PKR) [2]. Moreover, FMD Virion Protein 1 (VP1) has been specifically identified as a viral-origin IFN suppressor molecule by interacting with soluble resistancerelated calcium protein sorcin [3]. Since a key FMDV control method by host cells is suppression of IFNα production by FMDV-infected cells then exogenous treatment with IFNα or induction of endogenous IFN should help control FMD. Indeed, this vulnerability of FMDV to IFN has led to a novel viral disease control strategy using recombinant replication-defective human adenovirus 5 vector containing various species IFN genes. Results varied by species.","PeriodicalId":386740,"journal":{"name":"Journal of Vaccines, Immunology and Immunopathology","volume":"3 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121453755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terrestrial Paradise: Multiplex Immunoassay for Specific Pneumococcal Polysaccharide Antibodies 陆地天堂:多重免疫分析特异性肺炎球菌多糖抗体

Journal of Vaccines, Immunology and Immunopathology Pub Date : 2018-06-18 DOI: 10.29011/2575-789x.000130

G. Rijkers

{"title":"Terrestrial Paradise: Multiplex Immunoassay for Specific Pneumococcal Polysaccharide Antibodies","authors":"G. Rijkers","doi":"10.29011/2575-789x.000130","DOIUrl":"https://doi.org/10.29011/2575-789x.000130","url":null,"abstract":"Streptococcus pneumoniae is an encapsulated Grampositive, facultative anaerobic bacterium. It is a cause for major mucosal as well as invasive diseases, including otitis media, pneumonia, bacteremia, meningitis [1]. The WHO estimates that annually half a million children under five years of age die due to pneumococcal infections [2]. In elderly, a similar high burden of invasive pneumococcal disease is found [3]. A variety of diagnostic tests exists to identify the causative pneumococcal serotype in case of a suspected infection [4]. An indirect, but specific, method to investigate the involvement of S. pneumoniae in a given infectious disease is analysis of the serological response, i.e. the increase in serotype specific antibodies during the course of disease [5]. Streptococcus pneumoniae comes in 93 different serotypes. This is a challenge for the immune system to defend against, as antibodies which have been produced during an infection with a given serotype will not protect against an infection with a different serotype. This also presents a challenge for vaccine development, to select the most prevalent serotypes to be included into a vaccine and a challenge to medical immunologists, to measure the antibodies against all these different serotypes. The latter aspect is the topic of this Commentary. Before multiplex immunoassays were available, the only way to determine serotype specific pneumococcal antibodies was by Enzyme-Linked Immunosorbent Assay (ELISA) [6,7]. Ideally, 93 different ELISAs would be required to detect specific antibodies to all serotypes. In practice, the best that could be achieved was to test for antibodies to eight of the most common serotypes within the IgG, IgA, IgG1 and IgG2 (sub)class. This meant that every blood sample had to be tested on 32 ELISA plates, which was extremely time-consuming and also required quite a lot of material. Furthermore, because of the restricted dynamic range of ELISA, each sample had to be tested by serial dilution, limiting the number of samples to 8 per ELISA plate.","PeriodicalId":386740,"journal":{"name":"Journal of Vaccines, Immunology and Immunopathology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114890172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of an Epitope-Based Vaccine Against Human Immunodeficiency Virus (HIV) an in silicoApproach 基于表位的抗人类免疫缺陷病毒(HIV)疫苗的预测及其在硅中的应用

Journal of Vaccines, Immunology and Immunopathology Pub Date : 2018-05-30 DOI: 10.29011/2575-789x.000110

B. Salim, Nabila MutassimMurad, Zahra - Abdelmagid

{"title":"Prediction of an Epitope-Based Vaccine Against Human Immunodeficiency Virus (HIV) an in silicoApproach","authors":"B. Salim, Nabila MutassimMurad, Zahra - Abdelmagid","doi":"10.29011/2575-789x.000110","DOIUrl":"https://doi.org/10.29011/2575-789x.000110","url":null,"abstract":"Human Immunodeficiency Virus (HIV) is the causative agent of the Acquired Immunodeficiency Syndrome (AIDS). The disease is a major global public health threat that causes over 39 million deaths in 78 million cases ever since it was discovered. To date, there is no vaccine available for prevention, and most of the efforts were directed towards the conventional vaccines approaches. Thus, in the present study, an alternative newly emerging in silico approach for designing peptide-based vaccines has been sought. This immune informatics approach has several advantages regarding the safety, stability and the ease to manufacture. We designed a potential multi-epitope vaccine against HIV-1 purposely for the most endemic sub-Saharan African countries. Three essential structural genes that include envelope ( Env ), group specific antigen ( Gag ) and polymerase ( Pol ) protein sequences were retrieved from the databases, analyzed and verified through a total of 15 in silico tools. This resulted in nine antigenic and non-allergic potential epitopes capable to provoke both humoral and cell mediated immunity without induction of autoimmunity. These epitopes covered 76.57% of the world population with a high sequence conservancy varies from 80-97%. Furthermore, computational docking techniques were used to confirm the strong binding interactions of the epitopes with their specific HLA molecules. Albeit, the results are awaiting validations by in vitro and in vivo experiments, nonetheless, this study provides a useful insight for the developing of successful vaccines to prevent the devastating HIV infections.","PeriodicalId":386740,"journal":{"name":"Journal of Vaccines, Immunology and Immunopathology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125009041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Polymorphisms of Selected Antigen-Coding Genes of Theileria parva Isolates from Cattle in Southwestern Uganda 乌干达西南部牛细小芽孢杆菌分离株抗原编码基因的多态性

Journal of Vaccines, Immunology and Immunopathology Pub Date : 1900-01-01 DOI: 10.29011/2575-789x.000159

引用次数: 0

The Temptation of St. Anthony: Fighting against an Invisible Enemy in Times of Corona 圣安东尼的诱惑:在科罗娜时代对抗无形的敌人

Journal of Vaccines, Immunology and Immunopathology Pub Date : 1900-01-01 DOI: 10.29011/2575-789x.000153

G. Rijkers

引用次数: 0

Saint James: Reflections on a Long Road of Treatments from Rheumatoid Arthritis to COVID-19 圣詹姆斯:从类风湿关节炎到COVID-19漫长治疗之路的反思

Journal of Vaccines, Immunology and Immunopathology Pub Date : 1900-01-01 DOI: 10.29011/2575-789x.000161

引用次数: 0

Mass of Saint Gregory: Lymphocyte Transformation Tests as a Diagnostic Tool in Clinical Immunology 圣格雷戈里肿块:淋巴细胞转化试验作为临床免疫学诊断工具

Journal of Vaccines, Immunology and Immunopathology Pub Date : 1900-01-01 DOI: 10.29011/2575-789x.000160

引用次数: 0

Vaccines and Vaccinology in Latin America Conference Report 拉丁美洲会议报告中的疫苗和疫苗学

Journal of Vaccines, Immunology and Immunopathology Pub Date : 1900-01-01 DOI: 10.29011/2575-789x.000152

引用次数: 0

Fc Receptors on CD4+ T cells: A Role in Cytokine Storm and Antibody Dependent Enhancement CD4+ T细胞Fc受体:在细胞因子风暴和抗体依赖性增强中的作用

Journal of Vaccines, Immunology and Immunopathology Pub Date : 1900-01-01 DOI: 10.29011/2575-789x.000157

引用次数: 0