Biomolecular Concepts最新文献_第5页

NADPH-derived ROS generation drives fibrosis and endothelial-to-mesenchymal transition in systemic sclerosis: Potential cross talk with circulating miRNAs. NADPH 衍生的 ROS 生成推动了系统性硬化症的纤维化和内皮细胞向间质转化：与循环 miRNA 的潜在交叉作用。

Biomolecular Concepts Pub Date : 2022-02-21 DOI: 10.1515/bmc-2021-0023

Anna Maria Posadino, Gian Luca Erre, Annalisa Cossu, Costanza Emanueli, Ali H Eid, Angelo Zinellu, Gianfranco Pintus, Roberta Giordo

{"title":"NADPH-derived ROS generation drives fibrosis and endothelial-to-mesenchymal transition in systemic sclerosis: Potential cross talk with circulating miRNAs.","authors":"Anna Maria Posadino, Gian Luca Erre, Annalisa Cossu, Costanza Emanueli, Ali H Eid, Angelo Zinellu, Gianfranco Pintus, Roberta Giordo","doi":"10.1515/bmc-2021-0023","DOIUrl":"10.1515/bmc-2021-0023","url":null,"abstract":"Systemic sclerosis (SSc) is an immune disorder characterized by diffuse fibrosis and vascular abnormalities of the affected organs. Although the etiopathology of this disease is largely unknown, endothelial damage and oxidative stress appear implicated in its initiation and maintenance. Here, we show for the first time that circulating factors present in SSc sera increased reactive oxygen species (ROS) production, collagen synthesis, and proliferation of human pulmonary microvascular endothelial cells (HPMECs). The observed phenomena were also associated with endothelial to mesenchymal transition (EndMT) as indicated by decreased von Willebrand factor (vWF) expression and increased alpha-smooth muscle actin, respectively, an endothelial and mesenchymal marker. SSc-induced fibroproliferative effects were prevented by HPMECs exposition to the NADPH oxidase inhibitor diphenyleneiodonium, demonstrating ROS's causative role and suggesting their cellular origin. Sera from SSc patients showed significant changes in the expression of a set of fibrosis/EndMT-associated microRNAs (miRNA), including miR-21, miR-92a, miR-24, miR-27b, miR-125b, miR-29c, and miR-181b, which resulted significantly upregulated as compared to healthy donors sera. However, miR29b resulted downregulated in SSc sera, whereas no significant differences were found in the expression of miR-29a in the two experimental groups of samples. Taking together our data indicate NADPH oxidase-induced EndMT as a potential mechanism of SSc-associated fibrosis, suggesting fibrosis-associated miRNAs as potentially responsible for initiating and sustaining the vascular alterations observed in this pathological condition.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"11-24"},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9459349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to "Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents". “从百里香根中分离的细菌内生菌的植物生长促进特性，并研究它们作为生物肥料提高精油含量的作用”勘误表。

Biomolecular Concepts Pub Date : 2022-02-21 DOI: 10.1515/bmc-2021-0025

Mahmoud Soliman Abdel-Hamid, Amr Fouda, Hesham Kamal Abo El-Ela, Abbas A El-Ghamry, Saad El-Din Hassan

引用次数: 0

The epigenetic dimension of protein structure. 蛋白质结构的表观遗传维度。

Biomolecular Concepts Pub Date : 2022-02-21 DOI: 10.1515/bmc-2022-0006

Fodil Azzaz, Jacques Fantini

{"title":"The epigenetic dimension of protein structure.","authors":"Fodil Azzaz, Jacques Fantini","doi":"10.1515/bmc-2022-0006","DOIUrl":"https://doi.org/10.1515/bmc-2022-0006","url":null,"abstract":"Accurate prediction of protein structure is one of the most challenging goals of biology. The most recent achievement is AlphaFold, a machine learning method that has claimed to have solved the structure of almost all human proteins. This technological breakthrough has been compared to the sequencing of the human genome. However, this triumphal statement should be treated with caution, as we identified serious flaws in some AlphaFold models. Disordered regions are often represented by large loops that clash with the overall protein geometry, leading to unrealistic structures, especially for membrane proteins. In fact, AlphaFold comes up against the notion that protein folding is not solely determined by genomic information. We suggest that all parameters controlling the structure of a protein without being strictly encoded in its amino acid sequence should be coined \"epigenetic dimension of protein structure.\" Such parameters include for instance protein solvation by membrane lipids, or the structuration of disordered proteins upon ligand binding, but exclude sequence-encoded sites of post-translational modifications such as glycosylation. In our view, this paradigm is necessary to reconcile two opposite properties of living systems: beyond rigorous biological coding, evolution has given way to a certain level of uncertainty and anarchy.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39942345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Gamma-induced mutants of Bacillus and Streptomyces display enhanced antagonistic activities and suppression of the root rot and wilt diseases in pulses γ诱导的芽孢杆菌和链霉菌突变体对豆类根腐病和枯萎病表现出增强的拮抗活性和抑制作用

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0004

Ariyan Manikandan, I. Johnson,, N. Jaivel, R. Krishnamoorthy, M. Senthilkumar, R. Raghu, N. O. Gopal, P. Mukherjee, R. Anandham

{"title":"Gamma-induced mutants of Bacillus and Streptomyces display enhanced antagonistic activities and suppression of the root rot and wilt diseases in pulses","authors":"Ariyan Manikandan, I. Johnson,, N. Jaivel, R. Krishnamoorthy, M. Senthilkumar, R. Raghu, N. O. Gopal, P. Mukherjee, R. Anandham","doi":"10.1515/bmc-2022-0004","DOIUrl":"https://doi.org/10.1515/bmc-2022-0004","url":null,"abstract":"Abstract This study aims to increase Bacillus and Streptomyces antagonistic activity against the root rot and wilt diseases of pulses caused by Macrophomina phaseolina and Fusarium oxysporum f. sp. udum, respectively. To increase antagonistic action, Bacillus subtilis BRBac4, Bacillus siamensis BRBac21, and Streptomyces cavourensis BRAcB10 were subjected to random mutagenesis using varying doses of gamma irradiation (0.5–3.0 kGy). Following the irradiation, 250 bacterial colonies were chosen at random for each antagonistic strain and their effects against pathogens were evaluated in a plate assay. The ERIC, BOX, and random amplified polymorphic studies demonstrated a clear distinction between mutant and wild-type strains. When mutants were compared to wild-type strains, they showed improved plant growth-promoting characteristics and hydrolytic enzyme activity. The disease suppression potential of the selected mutants, B. subtilis BRBac4-M6, B. siamensisi BRBac21-M10, and S. cavourensis BRAcB10-M2, was tested in green gram, black gram, and red gram. The combined inoculation of B. siamensis BRBac21-M10 and S. cavourensis BRAcB10-M2 reduced the incidence of root rot and wilt disease. The same treatment also increased the activity of the defensive enzymes peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. These findings suggested that gamma-induced mutation can be exploited effectively to improve the biocontrol characteristics of Bacillus and Streptomyces. Following the field testing, a combined bio-formulation of these two bacteria may be utilised to address wilt and root-rot pathogens in pulses.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"103 - 118"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43083875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Targeted photoimmunotherapy for cancer 癌症的靶向光免疫疗法

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0010

A. Mussini, Eleonora Uriati, P. Bianchini, A. Diaspro, L. Cavanna, S. Abbruzzetti, C. Viappiani

引用次数: 5

Seaweeds’ pigments and phenolic compounds with antimicrobial potential 具有抗菌潜力的海藻色素和酚类化合物

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0003

Louisa Gomes, P. Monteiro, João Cotas, A. Gonçalves, Chantal Fernandes, T. Gonçalves, L. Pereira

引用次数: 13

Corticosterone potentiates ochratoxin A-induced microglial activation 皮质酮增强赭曲霉毒素a诱导的小胶质细胞活化

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0017

Anchana Chansawhang, S. Phochantachinda, Piya Temviriyanukul, B. Chantong

{"title":"Corticosterone potentiates ochratoxin A-induced microglial activation","authors":"Anchana Chansawhang, S. Phochantachinda, Piya Temviriyanukul, B. Chantong","doi":"10.1515/bmc-2022-0017","DOIUrl":"https://doi.org/10.1515/bmc-2022-0017","url":null,"abstract":"Abstract Microglial activation in the central nervous system (CNS) has been associated with brain damage and neurodegenerative disorders. Ochratoxin A (OTA) is a mycotoxin that occurs naturally in food and feed and has been associated with neurotoxicity, while corticosteroids are CNS’ physiological function modulators. This study examined how OTA affected microglia activation and how corticosteroids influenced microglial neuroinflammation. Murine microglial cells (BV-2) were stimulated by OTA, and the potentiation effects on OTA-induced inflammation were determined by corticosterone pre-treatment. Expressions of pro-inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) were determined. Phosphorylation of mitogen-activated protein kinases (MAPKs) was analyzed by western blotting. OTA significantly increased the mRNA expression of IL-6, TNF-α, IL-1β, and iNOS and also elevated IL-6 and NO levels. Corticosterone pre-treatment enhanced the neuroinflammatory response to OTA in a mineralocorticoid receptor (MR)-dependent mechanism, which is associated with increases in extracellular signal-regulated kinase (ERK) and p38 MAPK activation. In response to OTA, microglial cells produced pro-inflammatory cytokines and NO, while corticosterone increased OTA-induced ERK and p38 MAPK phosphorylation via MR. Findings indicated the direct role of OTA in microglia activation and neuroinflammatory response and suggested that low corticosterone concentrations in the brain exacerbated neurodegeneration.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"230 - 241"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48174037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The capture of host cell’s resources: The role of heat shock proteins and polyamines in SARS-COV-2 (COVID-19) pathway to viral infection 宿主细胞资源的捕获：热休克蛋白和多胺在SARS-COV-2（新冠肺炎）病毒感染途径中的作用

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0008

X. Makhoba, S. Makumire

{"title":"The capture of host cell’s resources: The role of heat shock proteins and polyamines in SARS-COV-2 (COVID-19) pathway to viral infection","authors":"X. Makhoba, S. Makumire","doi":"10.1515/bmc-2022-0008","DOIUrl":"https://doi.org/10.1515/bmc-2022-0008","url":null,"abstract":"Abstract The exposure of organisms and cells to unfavorable conditions such as increased temperature, antibiotics, reactive oxygen species, and viruses could lead to protein misfolding and cell death. The increased production of proteins such as heat shock proteins (HSPs) and polyamines has been linked to protein misfolding sequestration, thus maintaining, enhancing, and regulating the cellular system. For example, heat shock protein 40 (Hsp40) works hand in hand with Hsp70 and Hsp90 to successfully assist the newly synthesized proteins in folding properly. On the other hand, polyamines such as putrescine, spermidine, and spermine have been widely studied and reported to keep cells viable under harsh conditions, which are also involved in cell proliferation, differentiation, and growth. Polyamines are found in all living organisms, including humans and viruses. Some organisms have developed a mechanism to hijack mammalian host cell machinery for their benefit like viruses need polyamines for infection. Therefore, the role of HSPs and polyamines in SARS-CoV-2 (COVID-19) viral infection, how these molecules could delay the effectiveness of the current treatment in the market, and how COVID-19 relies on the host molecules for its successful infection are reviewed.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"220 - 229"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47479910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Electrophysiological study of the effects of side products of RuBi-GABA uncaging on GABAA receptors in cerebellar granule cells RuBi GABA开盖副产物对小脑颗粒细胞GABAA受体影响的电生理研究

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0022

E. Gatta, V. Bazzurro, E. Angeli, A. Salis, G. Damonte, A. Cupello, M. Robello, A. Diaspro

{"title":"Electrophysiological study of the effects of side products of RuBi-GABA uncaging on GABAA receptors in cerebellar granule cells","authors":"E. Gatta, V. Bazzurro, E. Angeli, A. Salis, G. Damonte, A. Cupello, M. Robello, A. Diaspro","doi":"10.1515/bmc-2022-0022","DOIUrl":"https://doi.org/10.1515/bmc-2022-0022","url":null,"abstract":"Abstract The study of the GABAA receptor itself and its pharmacology is of paramount importance for shedding light on the role of this receptor in the central nervous system. Caged compounds have emerged as powerful tools to support research in this field, as they allow to control, in space and time, the release of neurotransmitters enabling, for example, to map receptors’ distribution and dynamics. Here we focus on γ-aminobutyric acid (GABA)-caged compounds, particularly on a commercial complex called RuBi-GABA, which has high efficiency of uncaging upon irradiation at visible wavelengths. We characterized, by electrophysiological measurements, the effects of RuBi-GABA on GABAA receptors of rat cerebellar granule cells in vitro. In particular, we evaluated the effects of side products obtained after RuBi-GABA photolysis. For this purpose, we developed a procedure to separate the “RuBi-cage” from GABA after uncaging RuBi-GABA with a laser source; then, we compared electrophysiological measurements acquired with and without administering the RuBi-cage in the perfusing bath. In conclusion, to investigate the role of the “cage” molecules both near and far from the cell soma, we compared experiments performed changing the distance of the uncaging point from the cell.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"289 - 297"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47072426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology of human papillomavirus in pregnant women in Burkina Faso. 布基纳法索孕妇人乳头瘤病毒的分子流行病学研究。

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0026

Koudpoko Madeleine Kabre, Djénéba Ouermi, Théodora Mahoukèdè Zohoncon, Fatié Porzé Wilfried Traore, Ouamini Pulchérie De Prisca Gnoumou, Rogomenoma Alice Ouedraogo, Albert Théophane Yonli, Prosper Bado, Paul Ouedraogo, Teega-Wendé Clarisse Ouedraogo, Tampoula Edwige Yelemkoure, Punya Akouélé Kuassi-Kpede, Dorcas Obiri-Yeboah, Charlemagne Marie Ragnag-Néwendé Ouedraogo, Jacques Simpore

{"title":"Molecular epidemiology of human papillomavirus in pregnant women in Burkina Faso.","authors":"Koudpoko Madeleine Kabre, Djénéba Ouermi, Théodora Mahoukèdè Zohoncon, Fatié Porzé Wilfried Traore, Ouamini Pulchérie De Prisca Gnoumou, Rogomenoma Alice Ouedraogo, Albert Théophane Yonli, Prosper Bado, Paul Ouedraogo, Teega-Wendé Clarisse Ouedraogo, Tampoula Edwige Yelemkoure, Punya Akouélé Kuassi-Kpede, Dorcas Obiri-Yeboah, Charlemagne Marie Ragnag-Néwendé Ouedraogo, Jacques Simpore","doi":"10.1515/bmc-2022-0026","DOIUrl":"https://doi.org/10.1515/bmc-2022-0026","url":null,"abstract":"Introduction: Genital human papillomavirus (HPV) infection is widespread among sexually active individuals. Several factors may contribute to increased risk of infection in pregnant women. The objective of this study was to determine the high-risk (HR-HPV) and low-risk (LR-HPV) oncogenic HPV genotypes among pregnant women in Ouagadougou.Methodology: In this study, 100 endocervical samples were collected using a sterile swab on the sterile examination glove used during vaginal examination in pregnant women. DNA from each sample was amplified by PCR followed by hybridization using the HPV Direct Flow Chips kit detecting 36 HPV genotypes.Results: Twenty-three percent (23%) of pregnant women had HPV infection. Of the 36 genotypes tested, 29 genotypes had been identified with a predominance of HPV 52 (10.34%), HPV 35 (6.89%), and HPV 82 (6.89%) for high risk and HPV 43 (10.34%), HPV 44/55 (6.90%), and HPV 62/81 (6.89%) for low risk.Conclusion: HPV is common among pregnant women in Burkina Faso. However, the available vaccines do not cover the frequent genotypes found in this study. HPV could therefore constitute a threat for pregnant women and a risk of infection for the newborn.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"334-340"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10647724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3