Biomolecular Concepts最新文献_第6页

A photosensitizing fusion protein with targeting capabilities 一种具有靶向能力的光敏融合蛋白

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0014

S. Bruno, Marilena Margiotta, Marco Cozzolino, P. Bianchini, A. Diaspro, L. Cavanna, M. Tognolini, S. Abbruzzetti, C. Viappiani

引用次数: 3

Diffusion of molecules through nanopores under confinement: Time-scale bridging and crowding effects via Markov state model 约束下分子通过纳米孔的扩散:基于马尔可夫状态模型的时间尺度桥接和拥挤效应

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0019

I. Bodrenko, S. Milenkovic, M. Ceccarelli

{"title":"Diffusion of molecules through nanopores under confinement: Time-scale bridging and crowding effects via Markov state model","authors":"I. Bodrenko, S. Milenkovic, M. Ceccarelli","doi":"10.1515/bmc-2022-0019","DOIUrl":"https://doi.org/10.1515/bmc-2022-0019","url":null,"abstract":"Abstract Passive transport of molecules through nanopores is characterized by the interaction of molecules with pore internal walls and by a general crowding effect due to the constricted size of the nanopore itself, which limits the presence of molecules in its interior. The molecule–pore interaction is treated within the diffusion approximation by introducing the potential of mean force and the local diffusion coefficient for a correct statistical description. The crowding effect can be handled within the Markov state model approximation. By combining the two methods, one can deal with complex free energy surfaces taking into account crowding effects. We recapitulate the equations bridging the two models to calculate passive currents assuming a limited occupancy of the nanopore in a wide range of molecular concentrations. Several simple models are analyzed to clarify the consequences of the model. Eventually, a biologically relevant case of transport of an antibiotic molecule through a bacterial porin is used to draw conclusions (i) on the effects of crowding on transport of small molecules through biological channels, and (ii) to demonstrate its importance for modelling of cellular transport.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"207 - 219"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48543533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Is styrene competitive for dopamine receptor binding? 苯乙烯对多巴胺受体结合有竞争力吗？

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0016

E. De Santis, V. Minicozzi, G. Rossi, F. Stellato, S. Morante

引用次数: 0

Ion channels and neuronal excitability in polyglutamine neurodegenerative diseases 聚谷氨酰胺神经退行性疾病的离子通道与神经元兴奋性

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0018

Vladimir A. Martínez-Rojas, L. J. Juárez-Hernández, C. Musio

引用次数: 0

Subcellular elements responsive to the biomechanical activity of triple-negative breast cancer-derived small extracellular vesicles. 亚细胞因子对三阴性乳腺癌来源的细胞外小泡的生物力学活性的响应。

Biomolecular Concepts Pub Date : 2022-01-01 DOI: 10.1515/bmc-2022-0024

Beatrice Senigagliesi, Diana E Bedolla, Giovanni Birarda, Michele Zanetti, Marco Lazzarino, Lisa Vaccari, Pietro Parisse, Loredana Casalis

{"title":"Subcellular elements responsive to the biomechanical activity of triple-negative breast cancer-derived small extracellular vesicles.","authors":"Beatrice Senigagliesi, Diana E Bedolla, Giovanni Birarda, Michele Zanetti, Marco Lazzarino, Lisa Vaccari, Pietro Parisse, Loredana Casalis","doi":"10.1515/bmc-2022-0024","DOIUrl":"https://doi.org/10.1515/bmc-2022-0024","url":null,"abstract":"Abstract Triple-negative breast cancer (TNBC) stands out for its aggressive, fast spread, and highly metastatic behavior and for being unresponsive to the classical hormonal therapy. It is considered a disease with a poor prognosis and limited treatment options. Among the mechanisms that contribute to TNBC spreading, attention has been recently paid to small extracellular vesicles (sEVs), nano-sized vesicles that by transferring bioactive molecules to recipient cells play a crucial role in the intercellular communication among cancer, healthy cells, and tumor microenvironment. In particular, TNBC-derived sEVs have been shown to alter proliferation, metastasis, drug resistance, and biomechanical properties of target cells. To shed light on the molecular mechanisms involved in sEVs mediation of cell biomechanics, we investigated the effects of sEVs on the main subcellular players, i.e., cell membrane, cytoskeleton, and nuclear chromatin organization. Our results unveiled that TNBC-derived sEVs are able to promote the formation and elongation of cellular protrusions, soften the cell body, and induce chromatin decondensation in recipient cells. In particular, our data suggest that chromatin decondensation is the main cause of the global cell softening. The present study added new details and unveiled a novel mechanism of activity of the TNBC-derived sEVs, providing information for the efficient translation of sEVs to cancer theranostics.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"13 1","pages":"322-333"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antibodies targeting enzyme inhibition as potential tools for research and drug development. 靶向酶抑制的抗体是研究和药物开发的潜在工具。

Biomolecular Concepts Pub Date : 2021-12-31 DOI: 10.1515/bmc-2021-0021

José Manuel Pérez de la Lastra, Victoria Baca-González, Sergio González-Acosta, Patricia Asensio-Calavia, Andrea Otazo-Pérez, Antonio Morales-delaNuez

{"title":"Antibodies targeting enzyme inhibition as potential tools for research and drug development.","authors":"José Manuel Pérez de la Lastra, Victoria Baca-González, Sergio González-Acosta, Patricia Asensio-Calavia, Andrea Otazo-Pérez, Antonio Morales-delaNuez","doi":"10.1515/bmc-2021-0021","DOIUrl":"https://doi.org/10.1515/bmc-2021-0021","url":null,"abstract":"Antibodies have transformed biomedical research and are now being used for different experimental applications. Generally, the interaction of enzymes with their specific antibodies can lead to a reduction in their enzymatic activity. The effect of the antibody is dependent on its narrow i.e. the regions of the enzyme to which it is directed. The mechanism of this inhibition is rarely a direct combination of the antibodies with the catalytic site, but is rather due to steric hindrance, barring the substrate access to the active site. In several systems, however, the interaction with the antibody induces conformational changes on the enzyme that can either inhibit or enhance its catalytic activity. The extent of enzyme inhibition or enhancement is, therefore, a reflection of the nature and distribution of the various antigenic determinants on the enzyme molecule. Currently, the mode of action of many enzymes has been elucidated at the molecular level. We here review the molecular mechanisms and recent trends by which antibodies inhibit the catalytic activity of enzymes and provide examples of how specific antibodies can be useful for the neutralization of biologically active molecules.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"215-232"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39878139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. 促进植物生长的芽孢杆菌属根瘤菌防治枯萎病的意义。在维州faba L。

Biomolecular Concepts Pub Date : 2021-12-31 DOI: 10.1515/bmc-2021-0020

Mostafa Mohamed El-Sersawy, Saad El-Din Hassan, Abbas A El-Ghamry, Amr Mahmoud Abd El-Gwad, Amr Fouda

{"title":"Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L.","authors":"Mostafa Mohamed El-Sersawy, Saad El-Din Hassan, Abbas A El-Ghamry, Amr Mahmoud Abd El-Gwad, Amr Fouda","doi":"10.1515/bmc-2021-0020","DOIUrl":"https://doi.org/10.1515/bmc-2021-0020","url":null,"abstract":"Out of seven Fusarium spp. isolated from infected faba bean roots, two Fusarium oxysporum were selected and showed faba bean-wilt disease severity with percentages of 68% and 47% under greenhouse conditions. The F. oxysporum showed the highest wilt disease was selected to complete the current study. Three rhizobacterial strains were isolated and identified as Bacillus velezensis Vb1, B. paramycoides Vb3, and B. paramycoides Vb6. These strains showed the highest in-vitro antagonistic activity by the dual-culture method against selected F. oxysporum with inhibition percentages of 59±0.2, 46±0.3, and 52±0.3% for Vb1, Vb3, and Vb6, respectively. These rhizobacterial strains exhibit varied activity for nitrogen-fixing and phosphate-solubilizing. Moreover, these strains showed positive results for ammonia, HCN, and siderophores production. The phytohormones production (indole-3-acetic acid, ABA, benzyl, kinten, ziaten, and GA3) and secretion of various lytic enzymes were recorded by these strains with varying degrees. Under greenhouse conditions, the rhizobacterial strains Vb1, Vb3, Vb6, and their consortium can protect faba bean from wilt caused by F. oxysporum with percentages of 70, 60, 65, and 82%, respectively. Under field conditions, the inoculation with the rhizobacterial consortium (Vb1+Vb3+Vb6) significantly increases the growth performance of the F. oxysporum-infected faba bean plant and recorded the highest wilt protection (83.3%).","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"197-214"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39830718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Primary Erlotinib Resistance in a Patient with Non-Small Cell Lung Cancer Carrying Simultaneous Compound EGFR L718A, Q849H, and L858R Mutations. 同时携带复合EGFR L718A、Q849H和L858R突变的非小细胞肺癌患者的原发性厄洛替尼耐药

Biomolecular Concepts Pub Date : 2021-12-31 DOI: 10.1515/bmc-2021-0018

Hanifeh Mirtavoos-Mahyari, Elham Rismani, Alireza Sarkar Lotfabadi, Azizollah Abbasi Dezfouli, Kambiz Sheikhy, Mojtaba Mokhber Dezfuli, Jalal Heshmatnia

{"title":"Primary Erlotinib Resistance in a Patient with Non-Small Cell Lung Cancer Carrying Simultaneous Compound EGFR L718A, Q849H, and L858R Mutations.","authors":"Hanifeh Mirtavoos-Mahyari, Elham Rismani, Alireza Sarkar Lotfabadi, Azizollah Abbasi Dezfouli, Kambiz Sheikhy, Mojtaba Mokhber Dezfuli, Jalal Heshmatnia","doi":"10.1515/bmc-2021-0018","DOIUrl":"https://doi.org/10.1515/bmc-2021-0018","url":null,"abstract":"Nowadays, mutations in the epidermal growth factor receptor (EGFR) kinase domain are studied in targeted therapy of non-small cell lung cancer (NSCLC) with EGFR tyrosine kinase inhibitors including gefitinib and erlotinib. The present study reports a rare case of a patient harboring three simultaneous EGFR mutations (L718A, Q849H, and L858R). The development of erlotinib resistance was detected in the subsequent treatment. Using a computational approach, the current study investigated the conformational changes of wild-type and mutant EGFR's kinase domains in the interaction with erlotinib. Their binding modes with erlotinib were elucidated during molecular dynamics simulation, where higher fluctuations were detected in the mutated forms of the EGFR tyrosine kinase domain. Prediction of stability and functional effect of mutations revealed that amino acidic substitutions have decreased the protein stability as well as the binding affinity to erlotinib. These results may be useful for a recommendation of EGFR mutational analysis for patients with NSCLC carcinoma.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"164-174"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39924667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents. 从普通胸腺根中分离的细菌内生菌对植物生长的促进作用及其作为生物肥料提高挥发油含量的作用。

Biomolecular Concepts Pub Date : 2021-12-31 DOI: 10.1515/bmc-2021-0019

Mahmoud Soliman Abdel-Hamid, Amr Fouda, Hesham Kamal Abo El-Ela, Abbas A El-Ghamry, Saad El-Din Hassan

{"title":"Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents.","authors":"Mahmoud Soliman Abdel-Hamid, Amr Fouda, Hesham Kamal Abo El-Ela, Abbas A El-Ghamry, Saad El-Din Hassan","doi":"10.1515/bmc-2021-0019","DOIUrl":"https://doi.org/10.1515/bmc-2021-0019","url":null,"abstract":"The main objective of the current study was to improve the essential oil contents of Thymus vulgaris L. using bio-inoculation with bacterial endophytes. Therefore, out of fourteen endophytic bacterial isolates obtained from roots of T. vulgaris, five isolates were selected based on the highest nitrogen-fixation and phosphate solubilization activity and identified as: Bacillus haynesii T9r, Citrobacter farmeri T10r, Bacillus licheniformis T11r, Bacillus velezensis T12r, and Bacillus velezensis T13r. These five strains have been recorded as ammonia, hydrogen cyanide (HCN), siderophores, and indole-3-acetic acid (IAA) producers. These strains have the efficacy to fix-nitrogen by reduction of acetylene with values of 82.133±1.4-346.6±1.4 n-mole-C2H4/ml/24 h. The IAA, gibberellic acid, abscisic acid, benzyl, kinten, and ziaten production were confirmed using HPLC. Two strains of T11r and T13r showed the highest plant growth-promoting properties and were selected for bio-inoculation of T. vulgaris individually or in a consortium with different mineral fertilization doses (0, 50, 75, and 100%) under field conditions. The highest growth performance was attained with the endophytic consortium (T11r+T13r) in the presence of 100% mineral fertilization. The GC-MS analysis of thyme oil contents showed the presence of 23 various compounds with varying percentages and the thymol fraction represented the highest percentages (39.1%) in the presence of the bacterial consortium.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"175-196"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39830719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

SIT1 transporter as a potential novel target in treatment of COVID-19. SIT1转运蛋白作为治疗COVID-19的潜在新靶点

Biomolecular Concepts Pub Date : 2021-12-30 DOI: 10.1515/bmc-2021-0017

Sabina Semiz

{"title":"SIT1 transporter as a potential novel target in treatment of COVID-19.","authors":"Sabina Semiz","doi":"10.1515/bmc-2021-0017","DOIUrl":"https://doi.org/10.1515/bmc-2021-0017","url":null,"abstract":"Studies published earlier this year demonstrated the association of the solute carrier SLC6A20 gene with the risk and severity of COVID-19. The SLC6A20 protein product (Sodium-dependent Imino Transporter 1 (SIT1)) is involved in the transport of amino acids, including glycine. Here we summarized the results of recent studies demonstrating the interaction of SIT1 with the ACE2 receptor for SARS-CoV-2 as well as an observed association of SLC6A20 with the risk and traits of Type 2 diabetes (T2D). Recently, it was also proposed that SLC6A20 represents the novel regulator of glycine levels and that glycine has beneficial effects against the proinflammatory cytokine secretion induced by SARS-CoV-2 infection. Ivermectin, as a partial agonist of glycine-gated chloride channels, was also recently suggested to interfere with the COVID-19 cytokine storm by inducing the activation of glycine receptors. Furthermore, plasma glycine levels are found to be decreased in diabetic patients. Thus, further clinical trials are warranted to confirm the potential favorable effects of targeting the SIT1 transporter and glycine levels in the treatment of COVID-19, particularly for the severe case of disease associated with hyperglycemia, inflammation, and T2D. These findings suggest that SIT1 may potentially represent one of the missing pieces in the complex puzzle observed between these two pandemic diseases and the potential novel target for their efficient treatment.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"156-163"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39886556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5