Biomolecular Concepts最新文献_第7页

Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells. CXCL12/SDF-1在人骨髓基质细胞中差异表达mirna的表征

Biomolecular Concepts Pub Date : 2021-10-13 DOI: 10.1515/bmc-2021-0015

Matthew L Potter, Kathryn Smith, Sagar Vyavahare, Sandeep Kumar, Sudharsan Periyasamy-Thandavan, Mark Hamrick, Carlos M Isales, William D Hill, Sadanand Fulzele

{"title":"Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells.","authors":"Matthew L Potter, Kathryn Smith, Sagar Vyavahare, Sandeep Kumar, Sudharsan Periyasamy-Thandavan, Mark Hamrick, Carlos M Isales, William D Hill, Sadanand Fulzele","doi":"10.1515/bmc-2021-0015","DOIUrl":"https://doi.org/10.1515/bmc-2021-0015","url":null,"abstract":"Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"12 1","pages":"132-143"},"PeriodicalIF":0.0,"publicationDate":"2021-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10721143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Universal scanning-free initiation of eukaryote protein translation-a new normal. 真核生物蛋白翻译的普遍免扫描起始——新常态。

Biomolecular Concepts Pub Date : 2021-09-08 DOI: 10.1515/bmc-2021-0014

Saranya Auparakkitanon, Prapon Wilairat

引用次数: 0

3D host cell and pathogen-based bioassay development for testing anti-tuberculosis (TB) drug response and modeling immunodeficiency. 3D宿主细胞和基于病原体的生物测定开发用于检测抗结核(TB)药物反应和模拟免疫缺陷。

Biomolecular Concepts Pub Date : 2021-09-02 DOI: 10.1515/bmc-2021-0013

Shilpaa Mukundan, Rachana Bhatt, John Lucas, Matthew Tereyek, Theresa L Chang, Selvakumar Subbian, Biju Parekkadan

{"title":"3D host cell and pathogen-based bioassay development for testing anti-tuberculosis (TB) drug response and modeling immunodeficiency.","authors":"Shilpaa Mukundan, Rachana Bhatt, John Lucas, Matthew Tereyek, Theresa L Chang, Selvakumar Subbian, Biju Parekkadan","doi":"10.1515/bmc-2021-0013","DOIUrl":"https://doi.org/10.1515/bmc-2021-0013","url":null,"abstract":"Tuberculosis (TB) is a global health threat that affects 10 million people worldwide. Human Immunodeficiency Virus (HIV) remains one of the major contributors to the reactivation of asymptomatic latent tuberculosis (LTBI). Over the recent years, there has been a significant focus in developing in-vitro 3D models mimicking early events of Mycobacterium tuberculosis (Mtb) pathogenesis, especially formation of the granuloma. However, these models are low throughput and require extracellular matrix. In this article, we report the generation of a matrix-free 3D model, using THP-1 human monocyte/macrophage cells and mCherry-expressing Mycobacterium bovis BCG (Bacilli Camille Guérin), henceforth referred as 3D spheroids, to study the host cell-bacterial interactions. Using mCherry-intensity-based tracking, we monitored the kinetics of BCG growth in the 3D spheroids. We also demonstrate the application of the 3D spheroids for testing anti-TB compounds such as isoniazid (INH), rifampicin (RIF), as well as a host-directed drug, everolimus (EVR) as single and combinational treatments. We further established a dual infection 3D spheroid model by coinfecting THP-1 macrophages with BCG mCherry and pseudotype HIV. In this HIV-TB co-infection model, we found an increase in BCG mCherry growth within the 3D spheroids infected with HIV pseudotype. The degree of disruption of the granuloma was proportional to the virus titers used for co-infection. In summary, this 3D spheroid assay is an useful tool to screen anti-TB response of potential candidate drugs and can be adopted to model HIV-TB interactions.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"117-128"},"PeriodicalIF":0.0,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39380306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Insulin resistance is positively associated with plasma cathepsin D activity in NAFLD patients. NAFLD患者胰岛素抵抗与血浆组织蛋白酶D活性呈正相关。

Biomolecular Concepts Pub Date : 2021-08-09 DOI: 10.1515/bmc-2021-0011

Lingling Ding, Toon J I De Munck, Yvonne Oligschlaeger, Jef Verbeek, Ger H Koek, Tom Houben, Ronit Shiri-Sverdlov

{"title":"Insulin resistance is positively associated with plasma cathepsin D activity in NAFLD patients.","authors":"Lingling Ding, Toon J I De Munck, Yvonne Oligschlaeger, Jef Verbeek, Ger H Koek, Tom Houben, Ronit Shiri-Sverdlov","doi":"10.1515/bmc-2021-0011","DOIUrl":"https://doi.org/10.1515/bmc-2021-0011","url":null,"abstract":"Previous studies associated plasma cathepsin D (CTSD) activity with hepatic insulin resistance in overweight and obese humans. Insulin resistance is a major feature of non-alcoholic fatty liver disease (NAFLD) and is one of the multiple hits determining the progression towards non-alcoholic steatohepatitis (NASH). In line, we have previously demonstrated that plasma CTSD levels are increased in NASH patients. However, it is not known whether insulin resistance associates with plasma CTSD activity in NAFLD. To increase our understanding regarding the mechanisms by which insulin resistance mediates NAFLD, fifty-five liver biopsy or MRI-proven NAFLD patients (BMI>25kg/m2) were included to investigate the link between plasma CTSD activity to insulin resistance in NAFLD. We concluded that HOMA-IR and plasma insulin levels are independently associated with plasma CTSD activity in NAFLD patients (standardized coefficient β: 0.412, 95% Cl: 0.142~0.679, p=0.004 and standardized coefficient β: 0.495, 95% Cl: 0.236~0.758, p=0.000, respectively). Together with previous studies, these data suggest that insulin resistance may link to NAFLD via elevation of CTSD activity in plasma. As such, these data pave the way for testing CTSD inhibitors as a pharmacological treatment of NAFLD.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"110-115"},"PeriodicalIF":0.0,"publicationDate":"2021-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39294457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Retraction of: Alzheimer's and Danish dementia peptides induce cataract and perturb retinal architecture in rats. 阿尔茨海默氏症和丹麦痴呆肽的缩回诱导大鼠白内障和视网膜结构紊乱。

Biomolecular Concepts Pub Date : 2021-07-31 DOI: 10.1515/bmc-2021-0012

G Bhanuprakash Reddy, P Yadagiri Reddy, Avadhesha Surolia

引用次数: 0

Bcl2 negatively regulates Protective Immune Responses During Mycobacterial Infection. 分枝杆菌感染期间Bcl2负调控保护性免疫反应。

Biomolecular Concepts Pub Date : 2021-07-25 DOI: 10.1515/bmc-2021-0010

Aayushi Singh, Vandana Anang, Chaitenya Verma, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Upasana Bandyopadhyay, Attinder Chadha, Krishnamurthy Natarajan

{"title":"Bcl2 negatively regulates Protective Immune Responses During Mycobacterial Infection.","authors":"Aayushi Singh, Vandana Anang, Chaitenya Verma, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Upasana Bandyopadhyay, Attinder Chadha, Krishnamurthy Natarajan","doi":"10.1515/bmc-2021-0010","DOIUrl":"https://doi.org/10.1515/bmc-2021-0010","url":null,"abstract":"We previously reported that M. tb on its own as well as together with HIV inhibits macrophage apoptosis by upregulating the expression of Bcl2 and Inhibitor of Apoptosis (IAP). In addition, recent reports from our lab showed that stimulation of either macrophages or BMDCs results in the significant upregulation of Bcl2. In this report, we delineate the role of Bcl2 in mediating defense responses from dendritic cells (BMDCs) during mycobacterial infection. Inhibiting Bcl2 led to a significant decrease in intracellular bacterial burden in BMDCs. To further characterize the role of Bcl2 in modulating defense responses, we inhibited Bcl2 in BMDCs as well as human PBMCs to monitor their activation and functional status in response to mycobacterial infection and stimulation with M. tb antigen Rv3416. Inhibiting Bcl2 generated protective responses including increased expression of co-stimulatory molecules, oxidative burst, pro-inflammatory cytokine expression and autophagy. Finally, co-culturing human PBMCs and BMDCs with antigen-primed T cells increased their proliferation, activation and effector function. These results point towards a critical role for Bcl2 in regulating BMDCs defense responses to mycobacterial infection.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"94-109"},"PeriodicalIF":0.0,"publicationDate":"2021-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2021-0010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39218436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product. 泰国发酵大豆产品 Thua Nao 中蜡样芽孢杆菌噬菌体的发生率和特征。

Biomolecular Concepts Pub Date : 2021-07-02 DOI: 10.1515/bmc-2021-0009

Wallapat Phongtang, Ekachai Chukeatirote

{"title":"Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product.","authors":"Wallapat Phongtang, Ekachai Chukeatirote","doi":"10.1515/bmc-2021-0009","DOIUrl":"10.1515/bmc-2021-0009","url":null,"abstract":"Bacillus cereus is considered to be an important food poisoning agent causing diarrhea and vomiting. In this study, the occurrence of B. cereus bacteriophages in Thai fermented soybean products (Thua Nao) was studied using five B. cereus sensu lato indicator strains (four B. cereus strains and one B. thuringiensis strain). In a total of 26 Thua Nao samples, there were only two bacteriophages namely BaceFT01 and BaceCM02 exhibiting lytic activity against B. cereus. Morphological analysis revealed that these two bacteriophages belonged to the Myoviridae. Both phages were specific to B. cereus and not able to lyse other tested bacteria including B. licheniformis and B. subtilis. The two phages were able to survive in a pH range between 5 and 12. However, both phages were inactive either by treatment of 50°C for 2 h or exposure of UV for 2 h. It should be noted that both phages were chloroform-insensitive, however. This is the first report describing the presence of bacteriophages in Thua Nao products. The characterization of these two phages is expected to be useful in the food industry for an alternative strategy including the potential use of the phages as a biocontrol candidate against foodborne pathogenic bacteria.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2021-0009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Size-based Degradation of Therapeutic Proteins - Mechanisms, Modelling and Control. 基于尺寸的治疗蛋白降解-机制，建模和控制。

Biomolecular Concepts Pub Date : 2021-06-19 DOI: 10.1515/bmc-2021-0008

Rohit Bansal, Saurabh Kumar Jha, Niraj Kumar Jha

引用次数: 1

Pathophysiological Aspects of the Development of Abdominal Aortic Aneurysm with a Special Focus on Mitochondrial Dysfunction and Genetic Associations. 腹主动脉瘤发展的病理生理方面，特别关注线粒体功能障碍和遗传关联。

Biomolecular Concepts Pub Date : 2021-06-11 DOI: 10.1515/bmc-2021-0007

Volha I Summerhill, Vasily N Sukhorukov, Ali H Eid, Ludmila V Nedosugova, Igor A Sobenin, Alexander N Orekhov

{"title":"Pathophysiological Aspects of the Development of Abdominal Aortic Aneurysm with a Special Focus on Mitochondrial Dysfunction and Genetic Associations.","authors":"Volha I Summerhill, Vasily N Sukhorukov, Ali H Eid, Ludmila V Nedosugova, Igor A Sobenin, Alexander N Orekhov","doi":"10.1515/bmc-2021-0007","DOIUrl":"https://doi.org/10.1515/bmc-2021-0007","url":null,"abstract":"Abdominal aortic aneurysm (AAA) is a complex degenerative vascular disease, with considerable morbidity and mortality rates among the elderly population. The mortality of AAA is related to aneurysm expansion (the enlargement of the aortic diameter up to 30 mm and above) and the subsequent rupture. The pathogenesis of AAA involves several biological processes, including aortic mural inflammation, oxidative stress, vascular smooth muscle cell apoptosis, elastin depletion, and degradation of the extracellular matrix. Mitochondrial dysfunction was also found to be associated with AAA formation. The evidence accumulated to date supports a close relationship between environmental and genetic factors in AAA initiation and progression. However, a comprehensive pathophysiological understanding of AAA formation remains incomplete. The open surgical repair of AAA is the only therapeutic option currently available, while a specific pharmacotherapy is still awaited. Therefore, there is a great need to clarify pathophysiological cellular and molecular mechanisms underlying AAA formation that would help to develop effective pharmacological therapies. In this review, pathophysiological aspects of AAA development with a special focus on mitochondrial dysfunction and genetic associations were discussed.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"55-67"},"PeriodicalIF":0.0,"publicationDate":"2021-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2021-0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39102978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Polyphenols from Passiflora ligularis Regulate Inflammatory Markers and Weight Gain. 西番莲多酚调节炎症标志物和体重增加。

Biomolecular Concepts Pub Date : 2021-06-05 DOI: 10.1515/bmc-2021-0005

Jaime Angel-Isaza, Juan Carlos Carmona-Hernandez, William Narváez-Solarte, Clara Helena Gonzalez-Correa

{"title":"Polyphenols from Passiflora ligularis Regulate Inflammatory Markers and Weight Gain.","authors":"Jaime Angel-Isaza, Juan Carlos Carmona-Hernandez, William Narváez-Solarte, Clara Helena Gonzalez-Correa","doi":"10.1515/bmc-2021-0005","DOIUrl":"https://doi.org/10.1515/bmc-2021-0005","url":null,"abstract":"Weight-related disorders affect more than half of the adult population worldwide; they are also concomitant with a state of chronic low-grade inflammation manifesting in abnormal cytokine production. The present study evaluated the effect of polyphenol and flavonoid extract from Passiflora ligularis (granadilla) on low-grade inflammation and body weight in overweight Wistar rats. To induce weight-gain, rats were fed a chow diet with 30% sucrose water and supplemented with 2.0, 2.5, and 3.0 g/L polyphenol extracts (n = 16). The design was a 3 +1 factorial model performed for 42 days (granadilla polyphenols, 3 levels of supplementation, and 1 control group). In addition to total polyphenol and total flavonoid content, the major identified and quantified polyphenol, via UHPLC, was ferulic acid. Interleukin 6 (IL-6), and cytokine tumor necrosis factor-alpha (TNF-α) were evaluated in serum. A decline in the concentration of TNF-α and in weight-gain was found in P. ligularis (granadilla) groups treated with the 2.5 g/L dose. Consumption of polyphenol extracts from granadilla inhibits interleukin-activity as an indicator of inflammation and aids in body-weight control, considering similar food intake, in overweight Wistar rats.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"36-45"},"PeriodicalIF":0.0,"publicationDate":"2021-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2021-0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39081707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4