Biomolecular Concepts最新文献_第7页

Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents. 从普通胸腺根中分离的细菌内生菌对植物生长的促进作用及其作为生物肥料提高挥发油含量的作用。

Biomolecular Concepts Pub Date : 2021-12-31 DOI: 10.1515/bmc-2021-0019

Mahmoud Soliman Abdel-Hamid, Amr Fouda, Hesham Kamal Abo El-Ela, Abbas A El-Ghamry, Saad El-Din Hassan

{"title":"Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents.","authors":"Mahmoud Soliman Abdel-Hamid, Amr Fouda, Hesham Kamal Abo El-Ela, Abbas A El-Ghamry, Saad El-Din Hassan","doi":"10.1515/bmc-2021-0019","DOIUrl":"https://doi.org/10.1515/bmc-2021-0019","url":null,"abstract":"The main objective of the current study was to improve the essential oil contents of Thymus vulgaris L. using bio-inoculation with bacterial endophytes. Therefore, out of fourteen endophytic bacterial isolates obtained from roots of T. vulgaris, five isolates were selected based on the highest nitrogen-fixation and phosphate solubilization activity and identified as: Bacillus haynesii T9r, Citrobacter farmeri T10r, Bacillus licheniformis T11r, Bacillus velezensis T12r, and Bacillus velezensis T13r. These five strains have been recorded as ammonia, hydrogen cyanide (HCN), siderophores, and indole-3-acetic acid (IAA) producers. These strains have the efficacy to fix-nitrogen by reduction of acetylene with values of 82.133±1.4-346.6±1.4 n-mole-C2H4/ml/24 h. The IAA, gibberellic acid, abscisic acid, benzyl, kinten, and ziaten production were confirmed using HPLC. Two strains of T11r and T13r showed the highest plant growth-promoting properties and were selected for bio-inoculation of T. vulgaris individually or in a consortium with different mineral fertilization doses (0, 50, 75, and 100%) under field conditions. The highest growth performance was attained with the endophytic consortium (T11r+T13r) in the presence of 100% mineral fertilization. The GC-MS analysis of thyme oil contents showed the presence of 23 various compounds with varying percentages and the thymol fraction represented the highest percentages (39.1%) in the presence of the bacterial consortium.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"175-196"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39830719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

SIT1 transporter as a potential novel target in treatment of COVID-19. SIT1转运蛋白作为治疗COVID-19的潜在新靶点

Biomolecular Concepts Pub Date : 2021-12-30 DOI: 10.1515/bmc-2021-0017

Sabina Semiz

{"title":"SIT1 transporter as a potential novel target in treatment of COVID-19.","authors":"Sabina Semiz","doi":"10.1515/bmc-2021-0017","DOIUrl":"https://doi.org/10.1515/bmc-2021-0017","url":null,"abstract":"Studies published earlier this year demonstrated the association of the solute carrier SLC6A20 gene with the risk and severity of COVID-19. The SLC6A20 protein product (Sodium-dependent Imino Transporter 1 (SIT1)) is involved in the transport of amino acids, including glycine. Here we summarized the results of recent studies demonstrating the interaction of SIT1 with the ACE2 receptor for SARS-CoV-2 as well as an observed association of SLC6A20 with the risk and traits of Type 2 diabetes (T2D). Recently, it was also proposed that SLC6A20 represents the novel regulator of glycine levels and that glycine has beneficial effects against the proinflammatory cytokine secretion induced by SARS-CoV-2 infection. Ivermectin, as a partial agonist of glycine-gated chloride channels, was also recently suggested to interfere with the COVID-19 cytokine storm by inducing the activation of glycine receptors. Furthermore, plasma glycine levels are found to be decreased in diabetic patients. Thus, further clinical trials are warranted to confirm the potential favorable effects of targeting the SIT1 transporter and glycine levels in the treatment of COVID-19, particularly for the severe case of disease associated with hyperglycemia, inflammation, and T2D. These findings suggest that SIT1 may potentially represent one of the missing pieces in the complex puzzle observed between these two pandemic diseases and the potential novel target for their efficient treatment.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"156-163"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39886556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Combination therapy using TGF-β1 and STI-571 can induce apoptosis in BCR-ABL oncogene-expressing cells. TGF-β1与STI-571联合治疗可诱导BCR-ABL癌基因表达细胞凋亡。

Biomolecular Concepts Pub Date : 2021-10-26 DOI: 10.1515/bmc-2021-0016

Masoome Bakhshayesh, Ladan Hosseini Gohari, Mahmood Barati, Majid Safa

{"title":"Combination therapy using TGF-β1 and STI-571 can induce apoptosis in BCR-ABL oncogene-expressing cells.","authors":"Masoome Bakhshayesh, Ladan Hosseini Gohari, Mahmood Barati, Majid Safa","doi":"10.1515/bmc-2021-0016","DOIUrl":"https://doi.org/10.1515/bmc-2021-0016","url":null,"abstract":"The BCR-ABL oncogene is a tyrosine kinase gene that is over-expressed in CML. It inhibits the TGF-β1 signaling pathway. Due to resistance of cells to the tyrosine kinase inhibitor, STI-571, the combined effect of STI-571 and TGF-β1 on K562 cells was studied in the present research. Results revealed that the TGF-β1 cell signaling pathway, which is activated in K562 cells treated with TGF-β1, activates collective cell signaling pathways involved in survival and apoptosis. It is noteworthy that treating K562 cells with STI-571 triggered apoptotic pathways, accompanied by a reduction in proteins such as Bcl-xL, Bcl-2, p-AKT, p-Stat5, p-FOXO3, and Mcl-1 and an increase in the pro-apoptotic proteins PARP cleavage, and p27, leading to an increase in sub-G1 phase-arrested and Annexin-positive cells. Interestingly, the proliferation behavior of TGF-β1-induced cells was changed with the combination therapy, and STI-571-induced apoptosis was also prompted by this combination. Thus, combination treatment appears to promote sub-G1 cell cycle arrest compared to individually treated cells. Furthermore, it strongly triggered apoptotic signaling. In conclusion, TGF-β1 did not negatively impact the effect of STI-571, based on positive annexin cells, and AKT protein phosphorylation remains effective in apoptosis.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"144-155"},"PeriodicalIF":0.0,"publicationDate":"2021-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39562794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells. CXCL12/SDF-1在人骨髓基质细胞中差异表达mirna的表征

Biomolecular Concepts Pub Date : 2021-10-13 DOI: 10.1515/bmc-2021-0015

Matthew L Potter, Kathryn Smith, Sagar Vyavahare, Sandeep Kumar, Sudharsan Periyasamy-Thandavan, Mark Hamrick, Carlos M Isales, William D Hill, Sadanand Fulzele

{"title":"Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells.","authors":"Matthew L Potter, Kathryn Smith, Sagar Vyavahare, Sandeep Kumar, Sudharsan Periyasamy-Thandavan, Mark Hamrick, Carlos M Isales, William D Hill, Sadanand Fulzele","doi":"10.1515/bmc-2021-0015","DOIUrl":"https://doi.org/10.1515/bmc-2021-0015","url":null,"abstract":"Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":"12 1","pages":"132-143"},"PeriodicalIF":0.0,"publicationDate":"2021-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10721143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Universal scanning-free initiation of eukaryote protein translation-a new normal. 真核生物蛋白翻译的普遍免扫描起始——新常态。

Biomolecular Concepts Pub Date : 2021-09-08 DOI: 10.1515/bmc-2021-0014

Saranya Auparakkitanon, Prapon Wilairat

引用次数: 0

3D host cell and pathogen-based bioassay development for testing anti-tuberculosis (TB) drug response and modeling immunodeficiency. 3D宿主细胞和基于病原体的生物测定开发用于检测抗结核(TB)药物反应和模拟免疫缺陷。

Biomolecular Concepts Pub Date : 2021-09-02 DOI: 10.1515/bmc-2021-0013

Shilpaa Mukundan, Rachana Bhatt, John Lucas, Matthew Tereyek, Theresa L Chang, Selvakumar Subbian, Biju Parekkadan

{"title":"3D host cell and pathogen-based bioassay development for testing anti-tuberculosis (TB) drug response and modeling immunodeficiency.","authors":"Shilpaa Mukundan, Rachana Bhatt, John Lucas, Matthew Tereyek, Theresa L Chang, Selvakumar Subbian, Biju Parekkadan","doi":"10.1515/bmc-2021-0013","DOIUrl":"https://doi.org/10.1515/bmc-2021-0013","url":null,"abstract":"Tuberculosis (TB) is a global health threat that affects 10 million people worldwide. Human Immunodeficiency Virus (HIV) remains one of the major contributors to the reactivation of asymptomatic latent tuberculosis (LTBI). Over the recent years, there has been a significant focus in developing in-vitro 3D models mimicking early events of Mycobacterium tuberculosis (Mtb) pathogenesis, especially formation of the granuloma. However, these models are low throughput and require extracellular matrix. In this article, we report the generation of a matrix-free 3D model, using THP-1 human monocyte/macrophage cells and mCherry-expressing Mycobacterium bovis BCG (Bacilli Camille Guérin), henceforth referred as 3D spheroids, to study the host cell-bacterial interactions. Using mCherry-intensity-based tracking, we monitored the kinetics of BCG growth in the 3D spheroids. We also demonstrate the application of the 3D spheroids for testing anti-TB compounds such as isoniazid (INH), rifampicin (RIF), as well as a host-directed drug, everolimus (EVR) as single and combinational treatments. We further established a dual infection 3D spheroid model by coinfecting THP-1 macrophages with BCG mCherry and pseudotype HIV. In this HIV-TB co-infection model, we found an increase in BCG mCherry growth within the 3D spheroids infected with HIV pseudotype. The degree of disruption of the granuloma was proportional to the virus titers used for co-infection. In summary, this 3D spheroid assay is an useful tool to screen anti-TB response of potential candidate drugs and can be adopted to model HIV-TB interactions.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"117-128"},"PeriodicalIF":0.0,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39380306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Insulin resistance is positively associated with plasma cathepsin D activity in NAFLD patients. NAFLD患者胰岛素抵抗与血浆组织蛋白酶D活性呈正相关。

Biomolecular Concepts Pub Date : 2021-08-09 DOI: 10.1515/bmc-2021-0011

Lingling Ding, Toon J I De Munck, Yvonne Oligschlaeger, Jef Verbeek, Ger H Koek, Tom Houben, Ronit Shiri-Sverdlov

{"title":"Insulin resistance is positively associated with plasma cathepsin D activity in NAFLD patients.","authors":"Lingling Ding, Toon J I De Munck, Yvonne Oligschlaeger, Jef Verbeek, Ger H Koek, Tom Houben, Ronit Shiri-Sverdlov","doi":"10.1515/bmc-2021-0011","DOIUrl":"https://doi.org/10.1515/bmc-2021-0011","url":null,"abstract":"Previous studies associated plasma cathepsin D (CTSD) activity with hepatic insulin resistance in overweight and obese humans. Insulin resistance is a major feature of non-alcoholic fatty liver disease (NAFLD) and is one of the multiple hits determining the progression towards non-alcoholic steatohepatitis (NASH). In line, we have previously demonstrated that plasma CTSD levels are increased in NASH patients. However, it is not known whether insulin resistance associates with plasma CTSD activity in NAFLD. To increase our understanding regarding the mechanisms by which insulin resistance mediates NAFLD, fifty-five liver biopsy or MRI-proven NAFLD patients (BMI>25kg/m2) were included to investigate the link between plasma CTSD activity to insulin resistance in NAFLD. We concluded that HOMA-IR and plasma insulin levels are independently associated with plasma CTSD activity in NAFLD patients (standardized coefficient β: 0.412, 95% Cl: 0.142~0.679, p=0.004 and standardized coefficient β: 0.495, 95% Cl: 0.236~0.758, p=0.000, respectively). Together with previous studies, these data suggest that insulin resistance may link to NAFLD via elevation of CTSD activity in plasma. As such, these data pave the way for testing CTSD inhibitors as a pharmacological treatment of NAFLD.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"110-115"},"PeriodicalIF":0.0,"publicationDate":"2021-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39294457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Retraction of: Alzheimer's and Danish dementia peptides induce cataract and perturb retinal architecture in rats. 阿尔茨海默氏症和丹麦痴呆肽的缩回诱导大鼠白内障和视网膜结构紊乱。

Biomolecular Concepts Pub Date : 2021-07-31 DOI: 10.1515/bmc-2021-0012

G Bhanuprakash Reddy, P Yadagiri Reddy, Avadhesha Surolia

引用次数: 0

Bcl2 negatively regulates Protective Immune Responses During Mycobacterial Infection. 分枝杆菌感染期间Bcl2负调控保护性免疫反应。

Biomolecular Concepts Pub Date : 2021-07-25 DOI: 10.1515/bmc-2021-0010

Aayushi Singh, Vandana Anang, Chaitenya Verma, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Upasana Bandyopadhyay, Attinder Chadha, Krishnamurthy Natarajan

{"title":"Bcl2 negatively regulates Protective Immune Responses During Mycobacterial Infection.","authors":"Aayushi Singh, Vandana Anang, Chaitenya Verma, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Upasana Bandyopadhyay, Attinder Chadha, Krishnamurthy Natarajan","doi":"10.1515/bmc-2021-0010","DOIUrl":"https://doi.org/10.1515/bmc-2021-0010","url":null,"abstract":"We previously reported that M. tb on its own as well as together with HIV inhibits macrophage apoptosis by upregulating the expression of Bcl2 and Inhibitor of Apoptosis (IAP). In addition, recent reports from our lab showed that stimulation of either macrophages or BMDCs results in the significant upregulation of Bcl2. In this report, we delineate the role of Bcl2 in mediating defense responses from dendritic cells (BMDCs) during mycobacterial infection. Inhibiting Bcl2 led to a significant decrease in intracellular bacterial burden in BMDCs. To further characterize the role of Bcl2 in modulating defense responses, we inhibited Bcl2 in BMDCs as well as human PBMCs to monitor their activation and functional status in response to mycobacterial infection and stimulation with M. tb antigen Rv3416. Inhibiting Bcl2 generated protective responses including increased expression of co-stimulatory molecules, oxidative burst, pro-inflammatory cytokine expression and autophagy. Finally, co-culturing human PBMCs and BMDCs with antigen-primed T cells increased their proliferation, activation and effector function. These results point towards a critical role for Bcl2 in regulating BMDCs defense responses to mycobacterial infection.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"94-109"},"PeriodicalIF":0.0,"publicationDate":"2021-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2021-0010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39218436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product. 泰国发酵大豆产品 Thua Nao 中蜡样芽孢杆菌噬菌体的发生率和特征。

Biomolecular Concepts Pub Date : 2021-07-02 DOI: 10.1515/bmc-2021-0009

Wallapat Phongtang, Ekachai Chukeatirote

{"title":"Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product.","authors":"Wallapat Phongtang, Ekachai Chukeatirote","doi":"10.1515/bmc-2021-0009","DOIUrl":"10.1515/bmc-2021-0009","url":null,"abstract":"Bacillus cereus is considered to be an important food poisoning agent causing diarrhea and vomiting. In this study, the occurrence of B. cereus bacteriophages in Thai fermented soybean products (Thua Nao) was studied using five B. cereus sensu lato indicator strains (four B. cereus strains and one B. thuringiensis strain). In a total of 26 Thua Nao samples, there were only two bacteriophages namely BaceFT01 and BaceCM02 exhibiting lytic activity against B. cereus. Morphological analysis revealed that these two bacteriophages belonged to the Myoviridae. Both phages were specific to B. cereus and not able to lyse other tested bacteria including B. licheniformis and B. subtilis. The two phages were able to survive in a pH range between 5 and 12. However, both phages were inactive either by treatment of 50°C for 2 h or exposure of UV for 2 h. It should be noted that both phages were chloroform-insensitive, however. This is the first report describing the presence of bacteriophages in Thua Nao products. The characterization of these two phages is expected to be useful in the food industry for an alternative strategy including the potential use of the phages as a biocontrol candidate against foodborne pathogenic bacteria.","PeriodicalId":38392,"journal":{"name":"Biomolecular Concepts","volume":" ","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2021-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bmc-2021-0009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1