The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Q2 Biochemistry, Genetics and Molecular Biology

Biomolecular Concepts Pub Date : 2022-01-01 DOI:10.1515/bmc-2022-0009

A. Gradogna, J. M. Pardo, A. Carpaneto

引用次数: 1

Abstract

Abstract In the present work, we discuss the way in which the parallel application of the patch-clamp technique and the 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) fluorescence detection for recording luminal proton changes allows the functional characterization of nonelectrogenic potassium/proton vacuolar antiporters of the NHX (Na+/H+ exchanger) family. Moreover, we review the functional role of the tonoplast-specific phosphoinositide PI(3,5)P2, able to simultaneously inhibit the activity of NHXs and CLC-a transporters, whose coordinated action can play an important role in the water balance of plant cells.

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磷酸肌醇PI（3，5）P2抑制植物NHX质子/钾反转运蛋白的活性：一种新的电生理方法的优点

摘要在本工作中，我们讨论了膜片钳技术和2′，7′-双-（2-羧乙基）-5（6）-羧基荧光素（BCECF）荧光检测的并行应用，以记录管腔质子变化，从而能够对NHX（Na+/H+交换剂）家族的非电性钾/质子空泡反转运蛋白进行功能表征。此外，我们还综述了液泡膜特异性磷酸肌醇PI（3，5）P2的功能作用，它能够同时抑制NHX和CLC-a转运蛋白的活性，其协同作用可以在植物细胞的水分平衡中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular Concepts Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： BioMolecular Concepts is a peer-reviewed open access journal fostering the integration of different fields of biomolecular research. The journal aims to provide expert summaries from prominent researchers, and conclusive extensions of research data leading to new and original, testable hypotheses. Aspects of research that can promote related fields, and lead to novel insight into biological mechanisms or potential medical applications are of special interest. Original research articles reporting new data of broad significance are also welcome. Topics: -cellular and molecular biology- genetics and epigenetics- biochemistry- structural biology- neurosciences- developmental biology- molecular medicine- pharmacology- microbiology- plant biology and biotechnology.