Molecular epidemiology of human papillomavirus in pregnant women in Burkina Faso.

Q2 Biochemistry, Genetics and Molecular Biology

Biomolecular Concepts Pub Date : 2022-01-01 DOI:10.1515/bmc-2022-0026

Koudpoko Madeleine Kabre, Djénéba Ouermi, Théodora Mahoukèdè Zohoncon, Fatié Porzé Wilfried Traore, Ouamini Pulchérie De Prisca Gnoumou, Rogomenoma Alice Ouedraogo, Albert Théophane Yonli, Prosper Bado, Paul Ouedraogo, Teega-Wendé Clarisse Ouedraogo, Tampoula Edwige Yelemkoure, Punya Akouélé Kuassi-Kpede, Dorcas Obiri-Yeboah, Charlemagne Marie Ragnag-Néwendé Ouedraogo, Jacques Simpore

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引用次数: 3

Abstract

Introduction: Genital human papillomavirus (HPV) infection is widespread among sexually active individuals. Several factors may contribute to increased risk of infection in pregnant women. The objective of this study was to determine the high-risk (HR-HPV) and low-risk (LR-HPV) oncogenic HPV genotypes among pregnant women in Ouagadougou.

Methodology: In this study, 100 endocervical samples were collected using a sterile swab on the sterile examination glove used during vaginal examination in pregnant women. DNA from each sample was amplified by PCR followed by hybridization using the HPV Direct Flow Chips kit detecting 36 HPV genotypes.

Results: Twenty-three percent (23%) of pregnant women had HPV infection. Of the 36 genotypes tested, 29 genotypes had been identified with a predominance of HPV 52 (10.34%), HPV 35 (6.89%), and HPV 82 (6.89%) for high risk and HPV 43 (10.34%), HPV 44/55 (6.90%), and HPV 62/81 (6.89%) for low risk.

Conclusion: HPV is common among pregnant women in Burkina Faso. However, the available vaccines do not cover the frequent genotypes found in this study. HPV could therefore constitute a threat for pregnant women and a risk of infection for the newborn.

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布基纳法索孕妇人乳头瘤病毒的分子流行病学研究。

简介:生殖器人乳头瘤病毒(HPV)感染在性活跃的个体中广泛存在。有几个因素可能导致孕妇感染风险增加。本研究的目的是确定瓦加杜古孕妇中高危(HR-HPV)和低危(LR-HPV)致癌HPV基因型。方法:在本研究中，使用无菌棉签在孕妇阴道检查时使用的无菌检查手套上收集了100份宫颈内样本。每个样本的DNA通过PCR扩增，然后使用HPV Direct Flow Chips试剂盒检测36种HPV基因型进行杂交。结果:23%的孕妇有HPV感染。在检测的36个基因型中，29个基因型中高危型以HPV 52(10.34%)、HPV 35(6.89%)和HPV 82(6.89%)为主，低危型以HPV 43(10.34%)、HPV 44/55(6.90%)和HPV 62/81(6.89%)为主。结论:HPV在布基纳法索孕妇中很常见。然而，现有的疫苗不包括本研究中发现的常见基因型。因此，人乳头瘤病毒可能对孕妇构成威胁，并有感染新生儿的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular Concepts Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： BioMolecular Concepts is a peer-reviewed open access journal fostering the integration of different fields of biomolecular research. The journal aims to provide expert summaries from prominent researchers, and conclusive extensions of research data leading to new and original, testable hypotheses. Aspects of research that can promote related fields, and lead to novel insight into biological mechanisms or potential medical applications are of special interest. Original research articles reporting new data of broad significance are also welcome. Topics: -cellular and molecular biology- genetics and epigenetics- biochemistry- structural biology- neurosciences- developmental biology- molecular medicine- pharmacology- microbiology- plant biology and biotechnology.