Bioactive Carbohydrates and Dietary Fibre最新文献

{"title":"Gluten-free bulgur from selected chickpea cultivars: Nutritional and functional evaluation","authors":"Oguz Acar,&nbsp;Buket Cetiner,&nbsp;Seda Kulen,&nbsp;Ramazan Avcioglu","doi":"10.1016/j.bcdf.2025.100509","DOIUrl":"10.1016/j.bcdf.2025.100509","url":null,"abstract":"<div><div>This study aimed to assess mineral bioavailability and protein-derived energy (EC 1924/2006) in chickpea-based bulgur (Bahadir and Gokce), while providing a nutritious alternative suitable for celiac patients. The study also aims to assess mineral intake adequacy ratio (MIAR) from one serving of chickpea-based bulgur for adults aged 31–50 based on Dietary Reference Intakes (DRIs) while evaluating samples' chemical and cooking properties, total phenolic content (TPC), antioxidant activity, texture, and acceptability. Protein contents of bulgurs from Bahadir and Gokce were 33.4 % and 29.0 %, respectively, and both can be labeled as “high in protein” (EC 1924/2006). Mineral contents (except K and Ca) of Gokce were significantly higher than those of Bahadir (<em>p</em> &lt; 0.05). <em>In vitro</em> mineral bioavailability ranged from 42.3 % (Cu) to 91.8 % (Zn). The lowest MIAR by consumption of a portion bulgur 20.6, 30.3, 12.2, 4.9, 66.3, 11.7, 45.5, 12.4 and 2.6 % for Mg, P, K, Ca, Mn, Fe, Cu, Zn and Se, respectively. Bulgur production did not have any significant effect on TPC or antioxidant activity of cultivars (<em>p</em> &gt; 0.05). Furthermore, differences in bulgur texture between cultivars were not significant (<em>p</em> &gt; 0.05), while Bahadir scored higher in product acceptability traits (overall 5.13, indicating moderate consumer preference). Bulgur yield and cooking properties are similar in both cultivars. Bahadir shows higher protein content and mineral bioavailability, while Gokce is characterized by greater brightness. The study's findings on mineral bioavailability, MIAR, and protein-derived energy emphasize crucial nutritional indicators, supporting the use of chickpeas in bulgur as a cost-effective, wholesome, gluten-free option meeting dietary needs.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100509"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection against intestinal damage in female Drosophila melanogaster: Comparative contribution of arabinoxylan and its non-covalent/covalent complexes with catechin","authors":"Dima Atehli ,&nbsp;Tianyu Qi ,&nbsp;Rui Liu ,&nbsp;Wan Wang ,&nbsp;Qiaomei Zhu ,&nbsp;Yuanlin Sun ,&nbsp;Qingbin Guo ,&nbsp;Ji Kang","doi":"10.1016/j.bcdf.2025.100510","DOIUrl":"10.1016/j.bcdf.2025.100510","url":null,"abstract":"<div><div>Both arabinoxylan (AX) and catechin (CA) have shown promising therapeutic potential for inflammatory bowel disease (IBD). However, the efficacy of their covalent (S, CP) and non-covalent (PM) complexes against IBD remains largely unexplored. This study evaluated the therapeutic effects of AX, CA, and their respective complexes in a <em>Drosophila melanogaster</em> model of dextran sodium sulfate (DSS)-induced colitis. Results demonstrated that covalent AX-CA complexes generated by ultrasonic treatment provided the greatest therapeutic benefits, followed by those produced using cold plasma treatment. These complexes significantly improved survival rates, restored locomotor activity, enhanced metabolic rate, improved intestinal barrier function, markedly reduced the expression of genes associated with JAK/STAT signaling pathway, and concurrently upregulated the expression of genes within the Nrf2/Keap1 pathway.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100510"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional enhancement and biochemical effects of citrus waste dietary fibre in wistar albino rats fed fortified doughnuts","authors":"Dupe Temilade Otolowo ,&nbsp;Opeyemi Oluwayemisi Ayodele ,&nbsp;Josephine-Gold Jegede ,&nbsp;Omowumi Titilola Kayode","doi":"10.1016/j.bcdf.2025.100494","DOIUrl":"10.1016/j.bcdf.2025.100494","url":null,"abstract":"<div><div>Dietary fibre extracted manually from citrus wastes of orange, white grapefruit, red grapefruit, and lemon was used to fortify doughnuts as a potential solution to non-communicable diseases. Sixteen Wistar albino rats equally divided into four groups were fed the fibre-fortified doughnuts <em>ad libitum</em> for 28 days at 25, 15, and 0 % fibre inclusion; and another control group fed standard rat pellets. Nutritional, microbiological, and sensory properties were examined on doughnuts while the rats' weight and biochemical indices were assessed after feeding. Obtained data was statistically analysed using ANOVA and post-hoc tests. Proximate analysis of the doughnuts revealed moisture range (4.06–10.21 %), ash (1.87–2.09 %), fibre (0.01–0.26 %), fat (6.67–35.84 %), protein (10.17–16.37 %), and carbohydrate (46.81–64.68 %) with the significantly (p &lt; 0.05) highest crude fibre (0.26 %) in 25 % sample, showing improvement over regular doughnuts for gut health. Microbiological analysis showed safe TVB counts (7.0 × 10³–8.0 × 10⁴ CFU/g) and the absence of pathogens, supported by low moisture. Sensory assessment rated the fibre-fortified doughnuts in the range of 6–7 on a 9-point hedonic scale, indicating high acceptability. Average rats’ blood glucose ranged 61–79 mg/dL; 18.37–114.80 mg/dL in atherogenic lipid profiles (TC, TG, VLDL-C, and LDL-C) were lowest in 25 % fibre group and highest in HDL-C of the range (7.16–19.00 mg/dL), showing the most efficient in lowering blood glucose and atherogenic lipid profiles in the experimental rats compared to the control groups and better performance than the 15 % fibre doughnut. Thus, 25 % citrus fruit-waste-fibre-fortified doughnuts could be a nutritionally enhanced fast-food option to lower the risks of cardiovascular disease and diabetes.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100494"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of okra polysaccharides extract powder on mixolab attributes, physicochemical characteristics and in-vitro starch digestibility of wheat bread","authors":"Mehak Fatima ,&nbsp;Allah Rakha ,&nbsp;Muhammad Saeed ,&nbsp;Muhammad Shahid ,&nbsp;Filip Van Bockstaele","doi":"10.1016/j.bcdf.2025.100491","DOIUrl":"10.1016/j.bcdf.2025.100491","url":null,"abstract":"<div><div>The objective of this study was to assess the effect of extracted okra polysaccharides on the dough rheological properties, texture, antioxidant properties, and <em>in-vitro</em> starch digestion attributes of wheat bread. Different breads were prepared by incorporating okra polysaccharides extract powder (OPEP) at concentrations of 0.5 %, 1 %, and 1.5 % into wheat flour. The addition of OPEP in bread had a considerable effect on the torque-scale mixolab parameters C2, C3, and C5. Moreover, its incorporation led to an increase in the water absorption capacity and decreased dough stability time. Breads with varying concentrations of OPEP exhibited elevated levels of moisture content, protein, crude fiber and ash. However, the falling number for wheat flour decreased. Further, OPEP significantly impacted the volume, texture, and color of the bread. Overall, the bread hardness and water activity were lower on the 4th day of storage. The addition of OPEP increased the crumb color parameters. The color values of the crust remained mostly unaffected. Sensory attributes revealed that bread enriched with 1 % OPEP achieved the highest level of overall acceptability. Results also indicated that the incorporation of OPEP bread increased its antioxidant content. Rapidly digestible starch (RDS) and resistant starch (RS) contents were significantly affected by the addition of OPEP in wheat bread. Therefore, the findings of this investigation may potentially provide theoretical support for the development of a new standard regarding the industrial production of baked products.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100491"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional, pasting, rheological, crystalline, and thermal properties of red rice starch from Northern Indian sub-Himalayan belt: A comparative study with basmati rice","authors":"Rakshita Bhardwaj,&nbsp;Charanjiv Singh Saini","doi":"10.1016/j.bcdf.2025.100515","DOIUrl":"10.1016/j.bcdf.2025.100515","url":null,"abstract":"<div><div>Starch was extracted from both the red rice variety <em>Chhohartu</em> and basmati rice, which was compared based on their physicochemical, functional, pasting, rheological, crystalline, and thermal properties. <em>Chhohartu</em> starch exhibited higher total starch content (80 %) and amylose content (29.21 %), contributing to its higher gelatinization temperature (79.52 °C) and enhanced thermal stability. In contrast, basmati starch contained lower amylose (21.79 %) but exhibited higher solubility (20 %), highlighting its enhanced water-binding capacity. Water absorption capacity (WAC) and oil absorption capacity (OAC) were significantly higher in basmati starch (150 % and 162 %, respectively), whereas <em>Chhohartu</em> starch exhibited lower values (136 % and 158 %, respectively) due to its more compact granular structure. Pasting analysis revealed that basmati starch had a higher peak viscosity (3636 cP), hold viscosity (3221 cP), and final viscosity (5489 cP), indicating greater swelling and gelation capacity, while <em>Chhohartu</em> starch had a lower pasting temperature (79.95 °C) but higher setback viscosity (2278 cP), suggesting greater resistance to retrogradation. Rheological analysis confirmed that both starches exhibited shear-thinning behavior, with <em>Chhohartu</em> starch demonstrating higher yield stress (452 Pa) and complex viscosity (2960.86 Pa s), making it more resistant to structural breakdown under shear. The structural analysis indicated higher crystallinity in basmati starch (47.67 %) compared with <em>Chhohartu</em> (40.23 %), showing stronger hydrogen bonding in <em>Chhohartu</em> starch. These findings suggest that basmati starch is ideal for applications requiring higher swelling, viscosity, and hydration, while <em>Chhohartu</em> starch is better suited for high-temperature processing and formulations requiring enhanced structural integrity and retrogradation resistance.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100515"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and chemical characterization of polysaccharides from the red alga Plocamium cartilagineum (Linnaeus) P.S. Dixon 1967 with antiviral, anti-angiogenic, and anti-inflammatory activities","authors":"Bouchra Benhniya ,&nbsp;Fatima Zahra Karmil ,&nbsp;Fatima Lakhdar ,&nbsp;Zakaria Boujhoud ,&nbsp;Badr-ddine El Mouns ,&nbsp;Noreddine Rezzoum ,&nbsp;Nathalie Bourgougnon ,&nbsp;Samira Etahiri","doi":"10.1016/j.bcdf.2025.100499","DOIUrl":"10.1016/j.bcdf.2025.100499","url":null,"abstract":"<div><div>Marine algae are abundant in marine ecosystems and represent a valuable source of bioactive compounds. This study reports the extraction and characterization of the sulfated polysaccharide fraction (PCPS) from the Moroccan red alga <em>Plocamium cartilagineum</em>. The antiviral, anti-angiogenic, and anti-inflammatory activities of the cell wall sulfated polysaccharide (PCPS) were evaluated. The biochemical composition of PCPS was analyzed by quantifying total sugar, protein, uronic acid, polyphenol, and sulfate group contents. The structural characteristics of the extract were investigated using Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, X-ray diffraction, scanning electron microscopy, and high-performance liquid chromatography coupled with mass spectrometry. The extraction yield of PCPS was 11.63 ± 0.13 %, with sugars (72.06 ± 0.18 %) and sulfate groups (12.27 ± 0.06 %). FTIR confirmed the presence of sulfated polysaccharides, while XRD indicated a semi-crystalline to amorphous transition. HPLC-MS identified galactose, arabinose, and glucose, classifying PCPS as a heteropolysaccharide. PCPS exhibited strong antiviral activity against HSV-1 (EC₅₀ = 3.84 ± 2.8 μg/mL), anti-inflammatory effects by inhibiting phospholipase A₂ and elastase (IC₅₀ = 0.138 and 1.34 μg/mL, respectively), and anti-angiogenic properties, reducing vessel formation in the chorioallantoic membrane assay by 46.26 %. These findings highlight the pharmaceutical potential of <em>P. cartilagineum</em> polysaccharides.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100499"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic structural characterization of sulfated polysaccharides from the brown alga Hizikia fusiforme","authors":"Zhengqi Liu ,&nbsp;Menghui Wang ,&nbsp;Jinjin Li ,&nbsp;Zhe Zhao ,&nbsp;Xiaoming Guo ,&nbsp;Qingbin Guo ,&nbsp;Beiwei Zhu","doi":"10.1016/j.bcdf.2025.100498","DOIUrl":"10.1016/j.bcdf.2025.100498","url":null,"abstract":"<div><div><em>Hizikia fusiforme</em> sulfated polysaccharides (HFSP) have attracted considerable attention due to reported bioactivities, including immunomodulating effects. However, the overall structure of the sulfated polysaccharides from <em>H. fusiforme</em> remains unclear because of the high content of sulfate groups and the highly branched structure. In this study, sulfated polysaccharides from <em>H. fusiforme</em> were extracted using the CTAB method and purified using ion exchange chromatography and size exclusion chromatography. Their structural features were systematically analyzed using partial acid hydrolysis and desulfation process, followed by methylation analysis and 1D&amp;2D NMR spectroscopy. The molecular weight (Mw) of HFP was determined to be 74.50 kDa. The monosaccharide composition consisted of fucose (Fuc), galactose (Gal), xylose (Xyl), mannose (Man), and glucuronic acid (GlcA) in a ratio of 19.30: 25.22: 2.22: 19.82: 33.45. HFSP possessed a backbone composed of →2)-α-Man<em>p</em>-(1→ and →4)-β-GlcA<em>p</em>-(1→ units, with minor portions of →4)-β-Gal<em>p</em>-(1→ and →3)-β-Fuc<em>p</em>-(1→ units. Additionally, branch points are located at C-3 of →2)-α-Man<em>p</em>-(1→, C-6 of →4)-β-Gal<em>p</em>-(1→ and C-2 of →6)-β-Gal<em>p</em>-(1 → . The fragments of →6)-β-Gal<em>p</em>-4-SO₃-(1 → 2)-β-Gal<em>p</em>-4-SO₃-(1,6→ and →4)-β-GlcA<em>p</em>-(1 → 3)-α-Fuc<em>p</em>-(1 → 3)-α-Fuc<em>p</em>-(1→ have been newly found in the HFSP. The sulfated group were firstly identified in residues of α-Fuc<em>p</em>-2-SO₃-(1→, →4)-α-Fuc<em>p</em>-2,3-SO₃-(1→, →6)-β-Gal<em>p</em>-4-SO₃-(1→ and→2,6)-β-Gal<em>p</em>-4-SO₃-(1→, which are located on the outer branches of HFSP. This study provides a theoretical foundation for the structural characterization of sulfate polysaccharides and their derivatives.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100498"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gum Arabic regulates glucose metabolic enzymes through modification of hepatic phosphoenolpyruvate carboxykinase expression in diabetic rats","authors":"Saber Y. Adam ,&nbsp;Hamza A. Eltahir ,&nbsp;Fathi Abdallah Mohammed Ismaeel ,&nbsp;Reem H. Abdallah ,&nbsp;Adam O. Abaker ,&nbsp;Hassan H. Musa ,&nbsp;Hosameldeen Mohamed Husien ,&nbsp;Layla Ahmed Mohammed ,&nbsp;Demin Cai ,&nbsp;Abdelkareem A. Ahmed","doi":"10.1016/j.bcdf.2025.100490","DOIUrl":"10.1016/j.bcdf.2025.100490","url":null,"abstract":"<div><div>Diabetes mellitus (DM) is a chronic metabolic disease that becomes one of the most serious problems of modern times, due to its long-lasting health complications. Gluconeogenesis is a responsible for continued production of glucose by using glucose synthesis enzymes. However, the effect of Gum Arabic (GA) on phosphoenolpyruvate carboxykinase (PEPCK) enzymes has not been reported. This study aims to investigate the effect of GA on PEPCK enzymes in diabetes rats. A total of thirty male Sprague-Dawley rats were divided into 3 groups (n = 10 of each): control group; diabetic group (150 mg/kg Alloxan); and diabetic group which was given 10 % GA in drinking water for 8 weeks. The effect of GA on lipid profile, serum glucose, and liver function enzymes levels were investigated. Oxidative stress gene activities, hepatic glucose regulating-enzymes, G6p1 and PEPK mRNA expression were measured. The result revealed GA administration significantly decreased plasma total cholesterol, and LDL concentrations compared to the diabetic group. GA-treatment significantly decreased concentrations of serum glucose, leptin, and TNF-α, and increased serum insulin compared to the diabetic group. Furthermore, treatment with GA significantly ubregulated SOD, CAT and GSH-Px concentration compared to the diabetic group. Consequently, GA-treatment significantly downregulated the activity of PEPCK, and Glucose-6-phosphatase enzymes and ubregulated hepatic hexokinase and pyruvate kinase activities compared to the diabetic groups. Likewise, GA-treatment significantly down-regulated the PEPK and G6p1 protein expression in liver compared to the diabetic group. Our findings suggest that the administration of GA prevented diabetic behavior via modification of PEPCK expression in diabetes rats.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100490"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Jaspreet Garg ,&nbsp;Jaspreet Kaur","doi":"10.1016/j.bcdf.2025.100496","DOIUrl":"10.1016/j.bcdf.2025.100496","url":null,"abstract":"","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"34 ","pages":"Article 100496"},"PeriodicalIF":3.6,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Livaux® gold kiwifruit powder consumption at 600 mg per day for 28 days increases Faecalibacterium prausnitzii numbers and decreases bloating and hydrogenotrophic species numbers in healthy individuals, consistent with slow fermentation: A randomized controlled trial","authors":"Starin McKeen ,&nbsp;Susanne M. Henning ,&nbsp;Erin Lewis ,&nbsp;Malkanthi Evans ,&nbsp;Emma Graham ,&nbsp;Neville Jopson ,&nbsp;Jennifer Gu ,&nbsp;Doug Rosendale","doi":"10.1016/j.bcdf.2025.100468","DOIUrl":"10.1016/j.bcdf.2025.100468","url":null,"abstract":"<div><div>Kiwifruit is renowned for its benefits to laxation. Livaux® is a gold kiwifruit powder retaining whole fruit benefits, including high-methoxy pectin content. Livaux was previously shown to provide laxation support and increased faecal <em>F. prausnitzii</em>. <em>F. prausnitzii</em> is known to use high-methoxy pectin as substrate.</div><div>We aimed to determine the changes to laxation and faecal <em>F. prausnitzii</em> numbers from 600 mg of Livaux. We used a randomized, double-blinded, placebo-controlled parallel-arm study with 85 participants conducted across four North American sites, comparing 28 days of 600 mg Livaux consumption vs cellulose placebo. The study was powered for the primary outcome Complete Spontaneous Bowel Movements (CSBM).</div><div>Participants visited at screening, baseline and endpoint. Anthropometric measures, hematology, clinical chemistry and hematology and fecal samples for microbiome assessment were collected at baseline and endpoint. Clinical outcomes (CSBM, Bristol Stool Form Score (BSFS), spontaneous bowel movements (SBM) and participant-assessed constipation symptoms and quality of life), food diaries and adverse events were recorded daily throughout the study. Analysis of the intent-to-treat population assessed differences from baseline to endpoint using ANOVA with post-hoc Tukey's T-test, an assessment of change from baseline to days 7, 14, 21 and 28 using repeated measures ANCOVA.</div><div>Livaux supplemented participants showed significantly increased fecal <em>F. prausnitzii</em> relative abundance, whilst placebo did not. Participants also showed significantly improved CSBM, SBM, BSFS and constipation symptom and quality of life scores over baseline.</div><div>Livaux supplementation improved laxation and increased <em>F. prausnitzii</em> at the low dose of 600 mg.</div><div>This study was registered at <span><span>https://clinicaltrials.gov/study/NCT03462199</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"33 ","pages":"Article 100468"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}