EuPA Open Proteomics最新文献_第9页

De novo sequencing of two novel peptides homologous to calcitonin-like peptides, from skin secretion of the Chinese Frog, Odorrana schmackeri 中国蛙皮肤分泌物中两个与降钙素样肽同源的新肽的从头测序

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.008

Geisa P.C. Evaristo , Martijn W.H. Pinkse , Tianbao Chen , Lei Wang , Shabaz Mohammed , Albert J.R. Heck , Isabella Mathes , Friedrich Lottspeich , Chris Shaw , Juan Pablo Albar , Peter D.E.M. Verhaert

{"title":"De novo sequencing of two novel peptides homologous to calcitonin-like peptides, from skin secretion of the Chinese Frog, Odorrana schmackeri","authors":"Geisa P.C. Evaristo , Martijn W.H. Pinkse , Tianbao Chen , Lei Wang , Shabaz Mohammed , Albert J.R. Heck , Isabella Mathes , Friedrich Lottspeich , Chris Shaw , Juan Pablo Albar , Peter D.E.M. Verhaert","doi":"10.1016/j.euprot.2015.07.008","DOIUrl":"10.1016/j.euprot.2015.07.008","url":null,"abstract":"<div><p>An MS/MS based analytical strategy was followed to solve the complete sequence of two new peptides from frog (<em>Odorrana schmackeri</em>) skin secretion. This involved reduction and alkylation with two different alkylating agents followed by high resolution tandem mass spectrometry. <em>De novo</em> sequencing was achieved by complementary CID and ETD fragmentations of full-length peptides and of selected tryptic fragments. Heavy and light isotope dimethyl labeling assisted with annotation of sequence ion series. The identified primary structures are GCD[I/L]STCATHN[I/L]VNE[I/L]NKFDKSKPSSGGVGPESP-NH<sub>2</sub> and SCNLSTCATHNLVNELNKFDKSKPSSGGVGPESF-NH<sub>2</sub>, i.e. two carboxyamidated 34 residue peptides with an aminoterminal intramolecular ring structure formed by a disulfide bridge between Cys<sub>2</sub> and Cys<sub>7</sub>. Edman degradation analysis of the second peptide positively confirmed the exact sequence, resolving I/L discriminations. Both peptide sequences are novel and share homology with calcitonin, calcitonin gene related peptide (CGRP) and adrenomedullin from other vertebrates. Detailed sequence analysis as well as the 34 residue length of both <em>O. schmackeri</em> peptides, suggest they do not fully qualify as either calcitonins (32 residues) or CGRPs (37 amino acids) and may justify their classification in a novel peptide family within the calcitonin gene related peptide superfamily. Smooth muscle contractility assays with synthetic replicas of the S–S linked peptides on rat tail artery, uterus, bladder and ileum did not reveal myotropic activity.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"8 ","pages":"Pages 157-166"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.07.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54257350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A quality control of proteomic experiments based on multiple isotopologous internal standards 基于多同位素内标的蛋白质组学实验质量控制

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.010

Adele Bourmaud , Sebastien Gallien , Bruno Domon

引用次数: 10

Workflow for large-scale analysis of melanoma tissue samples 大规模分析黑色素瘤组织样本的工作流程

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.011

Maria E. Yakovleva , Charlotte Welinder , Yutaka Sugihara , Krzysztof Pawłowski , Melinda Rezeli , Elisabet Wieslander , Johan Malm , György Marko-Varga

引用次数: 6

Quantitative proteomics and transcriptomics reveals metabolic differences in attracting and non-attracting human-in-mouse glioma stem cell xenografts and stromal cells 定量蛋白质组学和转录组学揭示了吸引和非吸引人小鼠胶质瘤干细胞异种移植和基质细胞的代谢差异

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.06.006

Norelle C. Wildburger , Cheryl F. Lichti , Richard D. LeDuc , Mary Schmidt , Roger A. Kroes , Joseph R. Moskal , Carol L. Nilsson

引用次数: 6

Seeing beyond the tip of the iceberg: A deep analysis of the venome of the Brazilian Rattlesnake, Crotalus durissus terrificus 看到冰山一角之外:对巴西响尾蛇，Crotalus durissus terrificus毒液的深入分析

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.05.006

Rafael D. Melani , Gabriel D.T. Araujo , Paulo C. Carvalho , Livia Goto , Fábio C.S. Nogueira , Magno Junqueira , Gilberto B. Domont

引用次数: 26

Ten years of public proteomics data: How things have evolved, and where the next ten years should lead us 十年的公共蛋白质组学数据:事物是如何演变的，以及未来十年我们将走向何方

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.014

Kenneth Verheggen , Lennart Martens

引用次数: 3

EuPA’s tribute to Juan Pablo Albar EuPA向Juan Pablo Albar致敬

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.016

Concha Gil, Fernando Corrales, Garry Corthals, György Marko-Varga, Peter Verhaert

引用次数: 0

Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment 仪器平台和实验工作流程的实验室间评估，用于定量准确性和可重复性评估

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.06.001

Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Ardeshir Amirkhani , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesc Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Paul A. Haynes , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Christoph H. Borchers

{"title":"Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment","authors":"Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Ardeshir Amirkhani , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesc Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Paul A. Haynes , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Christoph H. Borchers","doi":"10.1016/j.euprot.2015.06.001","DOIUrl":"10.1016/j.euprot.2015.06.001","url":null,"abstract":"<div><p>The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"8 ","pages":"Pages 6-15"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54257641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Proteopathogen2, a database and web tool to store and display proteomics identification results in the mzIdentML standard Proteopathogen2是一个数据库和web工具，用于存储和显示mzIdentML标准中的蛋白质组学鉴定结果

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.04.002

Vital Vialas , Concha Gil

引用次数: 4

Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases 阿尔茨海默病和其他神经退行性疾病患者脑脊液和血液中总载脂蛋白E及其特异性亚型的定量分析

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.012

Melinda Rezeli , Henrik Zetterberg , Kaj Blennow , Ann Brinkmalm , Thomas Laurell , Oskar Hansson , György Marko-Varga

引用次数: 36