Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Ardeshir Amirkhani , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesc Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Paul A. Haynes , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Christoph H. Borchers
{"title":"仪器平台和实验工作流程的实验室间评估，用于定量准确性和可重复性评估","authors":"Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Ardeshir Amirkhani , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesc Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Paul A. Haynes , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Christoph H. Borchers","doi":"10.1016/j.euprot.2015.06.001","DOIUrl":null,"url":null,"abstract":"<div><p>The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"8 ","pages":"Pages 6-15"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.06.001","citationCount":"29","resultStr":"{\"title\":\"Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment\",\"authors\":\"Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Ardeshir Amirkhani , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesc Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Paul A. Haynes , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Christoph H. Borchers\",\"doi\":\"10.1016/j.euprot.2015.06.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.</p></div>\",\"PeriodicalId\":38260,\"journal\":{\"name\":\"EuPA Open Proteomics\",\"volume\":\"8 \",\"pages\":\"Pages 6-15\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.euprot.2015.06.001\",\"citationCount\":\"29\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"EuPA Open Proteomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212968515300039\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515300039","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
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