EuPA Open Proteomics最新文献_第10页

Long time storage (archiving) of peptide, protein and tryptic digest samples on disposable nano-coated polymer targets for MALDI MS 多肽、蛋白质和胰蛋白酶消化样品在一次性纳米包被聚合物靶上的长期储存(存档)

EuPA Open Proteomics Pub Date : 2015-09-01 DOI: 10.1016/j.euprot.2015.07.013

Stefan Bugovsky , Wolfgang Winkler , Werner Balika , Günter Allmaier

引用次数: 2

Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples 直接输注- sim是一种快速、可靠的复杂样品中绝对蛋白定量方法

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.03.001

Christina Looße , Sara Galozzi , Linde Debor , Mattijs K. Julsing , Bruno Bühler , Andreas Schmid , Katalin Barkovits , Thorsten Müller , Katrin Marcus

{"title":"Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples","authors":"Christina Looße , Sara Galozzi , Linde Debor , Mattijs K. Julsing , Bruno Bühler , Andreas Schmid , Katalin Barkovits , Thorsten Müller , Katrin Marcus","doi":"10.1016/j.euprot.2015.03.001","DOIUrl":"10.1016/j.euprot.2015.03.001","url":null,"abstract":"<div><p>Relative and absolute quantification of proteins in biological and clinical samples are common approaches in proteomics. Until now, targeted protein quantification is mainly performed using a combination of HPLC-based peptide separation and selected reaction monitoring on triple quadrupole mass spectrometers. Here, we show for the first time the potential of absolute quantification using a direct infusion strategy combined with single ion monitoring (SIM) on a Q Exactive mass spectrometer. By using complex membrane fractions of <em>Escherichia coli</em>, we absolutely quantified the recombinant expressed heterologous human cytochrome P450 monooxygenase 3A4 (CYP3A4) comparing direct infusion-SIM with conventional HPLC-SIM. Direct-infusion SIM revealed only 14.7% (±4.1 (s.e.m.)) deviation on average, compared to HPLC-SIM and a decreased processing and analysis time of 4.5min (that could be further decreased to 30s) for a single sample in contrast to 65min by the LC–MS method. Summarized, our simplified workflow using direct infusion-SIM provides a fast and robust method for quantification of proteins in complex protein mixtures.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"7 ","pages":"Pages 20-26"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54256825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue 假定癌症标志物S100A4在浸润性鳞状细胞癌阴茎组织中的分布分析

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.02.001

Brian Flatley , Chris Quaye , Elizabeth Johnson , Alex Freeman , Asif Muneer , Suks Minhas , Jennifer C. Paterson , Fawaz Musa , Peter Malone , Rainer Cramer

引用次数: 2

Investigation of heart proteome of different consomic mouse strains. Testing the effect of polymorphisms on the proteome-wide trans-variation of proteins 不同经济品系小鼠心脏蛋白质组的研究。测试多态性对蛋白质全蛋白质组反式变异的影响

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.03.002

Stefanie Forler , Oliver Klein , Sebastian Köhler , Peter N. Robinson , Henning Witt , Marc Sultan , Murat Eravci , Vera Regitz-Zagrosek , Hans Lehrach , Joachim Klose

引用次数: 0

Deciphering metabolic networks by blue native polyacrylamide gel electrophoresis: A functional proteomic exploration 用蓝色天然聚丙烯酰胺凝胶电泳破译代谢网络:功能蛋白质组学的探索

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.05.003

Christopher Auger, Nishma D. Appanna, Azhar Alhasawi, Vasu D. Appanna

{"title":"Deciphering metabolic networks by blue native polyacrylamide gel electrophoresis: A functional proteomic exploration","authors":"Christopher Auger, Nishma D. Appanna, Azhar Alhasawi, Vasu D. Appanna","doi":"10.1016/j.euprot.2015.05.003","DOIUrl":"10.1016/j.euprot.2015.05.003","url":null,"abstract":"<div><p>Metabolism is the consortium of reactions within a cell which directs a variety of processes including energy synthesis, signalling and the behaviour of a biological system. Metabolic networks, and more specifically the activity of enzymes within them, provide an accurate status of how cellular information is being executed. The performance of these networks and their ability to siphon metabolites in a number of directions may be the difference between a healthy and diseased state. Blue native polyacrylamide gel electrophoresis (BN-PAGE), owing to its simplicity and wide-ranging applications, permits the inspection of these nodules. The separation of proteins and enzyme complexes in their native format enables the exploration of enzymatic activity in metabolic networks via in-gel assays. These are quick, specific, and amenable to further studies. This electrophoretic technology not only enables the visualization of enzymatic efficacy but reveals the crosstalk among enzymes and their interactions with other organellar partners.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"7 ","pages":"Pages 64-72"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54257581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Detecting significant changes in protein abundance 检测蛋白质丰度的显著变化

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.02.002

Kai Kammers , Robert N. Cole , Calvin Tiengwe , Ingo Ruczinski

引用次数: 212

Quantitative analysis of the erythrocyte membrane proteins in polycythemia vera patients treated with hydroxycarbamide 羟基脲治疗真性红细胞增多症患者红细胞膜蛋白的定量分析

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.04.001

Darshana Kottahachchi , Lallindra Gooneratne , Anil Jayasekera , Dorota Muth-Pawlak , Robert Moulder , Susumu Y. Imanishi , Ari Ariyaratne , Anne Rokka , Garry L. Corthals

引用次数: 5

Low molecular weight peptides derived from sarcoplasmic proteins produced by an autochthonous starter culture in a beaker sausage model 在烧杯香肠模型中，由本地发酵剂培养产生的肌浆蛋白衍生的低分子量肽

EuPA Open Proteomics Pub Date : 2015-06-01 DOI: 10.1016/j.euprot.2015.05.001

Constanza M. López , Miguel A. Sentandreu , Graciela M. Vignolo , Silvina G. Fadda

{"title":"Low molecular weight peptides derived from sarcoplasmic proteins produced by an autochthonous starter culture in a beaker sausage model","authors":"Constanza M. López , Miguel A. Sentandreu , Graciela M. Vignolo , Silvina G. Fadda","doi":"10.1016/j.euprot.2015.05.001","DOIUrl":"10.1016/j.euprot.2015.05.001","url":null,"abstract":"<div><p>This study focuses on meat protein degradation by different starter cultures. Sausage models inoculated with <em>Lactobacillus curvatus</em> CRL705 and <em>Staphylococcus vitulinus</em> GV318 alone and as a mixture were incubated 10 days at 22°C. Low molecular weight peptides (<3kDa) derived from sarcoplasmic proteins were analyzed by a peptidomic approach. A diverse number of protein fragments were identified. The greatest peptides diversity was obtained when the mixed starter culture was present. Peptides mainly arose from myoglobin, creatine-kinase, glyceraldehyde-3-phosphate-dehydrogenase and fructose-biphosphate-aldolase (ALDOA). ALDOA hydrolysis was attributed to the mixed starter culture; the released peptides could act as biomarkers for a specific sausage technology.</p></div><div><h3>Significance</h3><p>The selection of a specific autochthonous starter culture guarantees the hygiene and typicity of fermented sausages. The identification of new peptides as well as new target proteins by means of peptidomics represents a significant step toward the elucidation of the role of microorganisms in meat proteolysis. Moreover, these peptides may be further used as biomarkers capable to certify the use of the applied autochthonous starter culture described here.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"7 ","pages":"Pages 54-63"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54257544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Evaluation of phosphopeptide enrichment strategies for quantitative TMT analysis of complex network dynamics in cancer-associated cell signalling 评估用于定量TMT分析癌症相关细胞信号传导复杂网络动力学的磷酸肽富集策略

EuPA Open Proteomics Pub Date : 2015-03-01 DOI: 10.1016/j.euprot.2015.01.002

Benedetta Lombardi , Nigel Rendell , Mina Edwards , Matilda Katan , Jasminka Godovac Zimmermann

引用次数: 14

Multidimensional LC–MS/MS analysis of liver proteins in rat, mouse and human microsomal and S9 fractions 大鼠、小鼠和人微粒体及S9部分肝脏蛋白的多维LC-MS /MS分析

EuPA Open Proteomics Pub Date : 2015-03-01 DOI: 10.1016/j.euprot.2015.01.003

Makan Golizeh, Christina Schneider, Leanne B. Ohlund, Lekha Sleno

引用次数: 9