假定癌症标志物S100A4在浸润性鳞状细胞癌阴茎组织中的分布分析

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2015-06-01 DOI:10.1016/j.euprot.2015.02.001

Brian Flatley , Chris Quaye , Elizabeth Johnson , Alex Freeman , Asif Muneer , Suks Minhas , Jennifer C. Paterson , Fawaz Musa , Peter Malone , Rainer Cramer

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引用次数: 2

摘要

基于质谱的蛋白质组学方法首次用于发现新的阴茎癌生物标志物。采用MALDI质谱成像获得阴茎鳞状细胞癌的原位生物分子质谱，并与良性上皮质谱进行比较。我们检测了癌变组织和良性组织区域的光谱，以确定最能区分正常上皮细胞和侵袭性鳞状上皮细胞的质谱峰，为S100A4存在差异表达提供了重要证据。免疫组织化学证实S100A4在侵袭性但非良性上皮细胞亚群中呈阳性染色。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue

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Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue

MS-based proteomic methods were utilised for the first time in the discovery of novel penile cancer biomarkers. MALDI MS imaging was used to obtain the in situ biomolecular MS profile of squamous cell carcinoma of the penis which was then compared to benign epithelial MS profiles. Spectra from cancerous and benign tissue areas were examined to identify MS peaks that best distinguished normal epithelial cells from invasive squamous epithelial cells, providing crucial evidence to suggest S100A4 to be differentially expressed. Verification by immunohistochemistry resulted in positive staining for S100A4 in a sub-population of invasive but not benign epithelial cells.

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来源期刊

EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

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审稿时长

103 days