看到冰山一角之外:对巴西响尾蛇，Crotalus durissus terrificus毒液的深入分析

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2015-09-01 DOI:10.1016/j.euprot.2015.05.006

Rafael D. Melani , Gabriel D.T. Araujo , Paulo C. Carvalho , Livia Goto , Fábio C.S. Nogueira , Magno Junqueira , Gilberto B. Domont

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引用次数: 26

摘要

蛇毒蛋白成分的完整表征是对其生物学背景的全系统理解的要求。在这项工作中，我们使用不同的自下而上的方法提供了Crotalus durissus terrificus毒液的深层蛋白质组学特征。我们鉴定的蛋白质家族比以前报道的所有鉴定的总和多五倍以上。我们首次在该亚种中发现了三个新的毒素家族:CRISP、phospholipase-B和SVVEGF。这项工作还描述了存在于毒液中的毒素合成和加工调节的蛋白质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Seeing beyond the tip of the iceberg: A deep analysis of the venome of the Brazilian Rattlesnake, Crotalus durissus terrificus

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Seeing beyond the tip of the iceberg: A deep analysis of the venome of the Brazilian Rattlesnake, Crotalus durissus terrificus

The complete characterization of the snake venom protein components is a requirement for a systems-wide understanding of their biological context. In this work, we provide a deep proteomic characterization of Crotalus durissus terrificus venom using different bottom-up approaches. We identified more than five times more protein families than the sum of all identifications previously reported. For the first time in this sub-species, we report the identification of three new toxin families: CRISP, phospholipase-B, and SVVEGF. This work also describes proteins involved in regulation of toxin synthesis and processing that are present in venom.

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来源期刊

EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

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审稿时长

103 days