Food and Waterborne Parasitology最新文献

{"title":"Detection of Sarcocystis hominis, Sarcocystis bovifelis, Sarcocystis cruzi, Sarcocystis hirsuta and Sarcocystis sigmoideus sp. nov. in carcasses affected by bovine eosinophilic myositis","authors":"Selene Rubiola , Gastón Moré , Tiziana Civera , Andrew Hemphill , Caroline F. Frey , Walter Basso , Irene Colasanto , Davide Vercellino , Marta Fidelio , Mauro Lovisone , Francesco Chiesa","doi":"10.1016/j.fawpar.2024.e00220","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00220","url":null,"abstract":"<div><p>Bovine eosinophilic myositis is an inflammatory myopathy characterized by multiple focal or diffuse grey to green patches leading to condemnation of affected carcasses. Although its etiology is still uncertain, there is evidence that <em>Sarcocystis</em> species may play a role in the development of eosinophilic myositis. The goal of the present study was to identify <em>Sarcocystis</em> spp. in intralesional and extralesional tissues of condemned cattle carcasses, in order to evaluate the possible role of different bovine <em>Sarcocystis</em> spp. in the etiology of bovine eosinophilic myositis. Muscle samples (<em>n</em> = 100) of 26 affected carcasses were collected in Northern Italy. One to five samples with lesions and two aliquots of tissue without lesions were collected from each carcass; lesions were grossly categorized in green focal lesions and green diffuse patches. Genomic DNA was extracted and analyzed by multiplex-PCR targeting different <em>Sarcocystis</em> spp. Unidentified species were characterized morphologically (light microscopy, histology), ultrastructurally (scanning and transmission electron microscopy) and on the molecular level (complete 18S rRNA gene and partial <em>cox1</em> gene sequencing). A bovine eosinophilic myositis prevalence of 0.017% was visually assessed by routine carcass inspection between 2014 and 2019 in Italy (184/1,108,150 slaughtered cattle). Out of 26 carcasses, 25 revealed the presence of at least one <em>Sarcocystis</em> species (96.2%). The presence of <em>Sarcocystis</em> spp. DNA was significantly more frequent in intralesional than in extralesional samples. Considering the different species, <em>Sarcocystis bovifelis</em> and <em>Sarcocystis hominis</em> were significantly more frequent in intralesional (41.7% and 50%, respectively) than in extralesional samples (1.9% and 15.4%, respectively), while there was no significant difference between the presence of <em>Sarcocystis cruzi</em> and <em>Sarcocystis hirsuta</em> in intralesional (27.1% and 2.1%, respectively) and extralesional (30.8% and 1.9%, respectively) samples. The presence of an unnamed <em>Sarcocystis</em> sp. showing thick-walled (3.7–5.4 μm) cysts with densely packed, flattened, undulating and narrow protrusions, which showed an S-shape in side view, was recorded in the diaphragm of two carcasses. Genomic DNA from individual sarcocysts isolated from the diaphragm was successfully amplified and further sequenced. Sequence comparison revealed <94.6% and 83.4% identity at 18S rRNA and <em>cox1</em> genes, respectively, with other named <em>Sarcocystis</em> spp., while the phylogenetic analysis clearly separated the unnamed <em>Sarcocystis</em> sp. from the other <em>Sarcocystis</em> spp. using cattle as intermediate hosts. The present study contributes to the understanding of the importance of different <em>Sarcocystis</em> spp. in the pathogenesis of bovine eosinophilic myositis. The results emphasize the association of <","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"34 ","pages":"Article e00220"},"PeriodicalIF":2.5,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000027/pdfft?md5=19a87d4e20e31fa3411277713f06a6a2&pid=1-s2.0-S2405676624000027-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139549106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular phylogenetic analysis of Echinococcus multilocularis from horses raised in Canada or Japan, using mitochondrial cytochrome b gene–targeted PCR","authors":"Tatsuro Hifumi ,&nbsp;Tetsuya Tanaka ,&nbsp;Ichinosuke Suzu ,&nbsp;Miho Sato ,&nbsp;Kohei Akioka ,&nbsp;Chiaki Fujimata ,&nbsp;Ryohei Shinkai ,&nbsp;Tsutomu Maeda ,&nbsp;Kodai Kusakisako ,&nbsp;Hiromi Ikadai ,&nbsp;Noriaki Miyoshi","doi":"10.1016/j.fawpar.2024.e00219","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00219","url":null,"abstract":"<div><p>Alveolar echinococcosis is a zoonotic disease caused by a larval-stage <em>Echinococcus multilocularis</em> infection. Geographical haplotyping targeting the parasite's mitochondrial cytochrome <em>b</em> (<em>cob</em>) gene has been reported for isolates from definitive and intermediate hosts (wild canids and rodents); however, there are limited reports on strain typing for the dead-end host, the horse, which could act as a sentinel for E<em>. multilocularis</em>. Accordingly, we investigated the diversity of <em>E. multilocularis</em> in isolates obtained from slaughtered Japanese and Canadian horses originating from the Iburi and Hidaka regions in Hokkaido and from Alberta, respectively, with PCR and haplogroup analyses targeting <em>cob</em> gene sequences obtained. Seventy horses were diagnosed with alveolar echinococcosis based on histopathology and <em>cob</em>-gene PCR testing. The <em>E. multilocularis</em> detected in these horses was classified as either an Asian (for Hokkaido-raised horses) or a European (for Alberta-raised horses) haplogroup, based on the obtained <em>cob</em>-gene sequence analysis. In addition, haplotype network analysis revealed that <em>E. multilocularis</em> isolated from Hokkaido-raised horses is highly homologous to Kazakhstan isolates, and <em>E. multilocularis</em> isolated from Alberta-raised horses is highly homologous to Austrian isolates. The results of this study suggest that <em>cob</em>-gene-targeted PCR analysis could be useful for the geographical genetic characterization of <em>E. multilocularis</em> isolated from horses.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"34 ","pages":"Article e00219"},"PeriodicalIF":2.5,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000015/pdfft?md5=737cbd62f9e959c8ccc698f5e2d0de53&pid=1-s2.0-S2405676624000015-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139480174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Cryptosporidium parvum and Blastocystis hominis subtype ST3 in Cholga mussel and treated sewage: Preliminary evidence of fecal contamination in harvesting area","authors":"P. Suarez ,&nbsp;A. Vallejos-Almirall ,&nbsp;I. Fernández ,&nbsp;I. Gonzalez-Chavarria ,&nbsp;J.L. Alonso ,&nbsp;G. Vidal","doi":"10.1016/j.fawpar.2023.e00214","DOIUrl":"https://doi.org/10.1016/j.fawpar.2023.e00214","url":null,"abstract":"<div><p><em>Cryptosporidium parvum</em> and <em>Blastocystis hominis</em> are foodborne parasites known for causing diarrhea. They accumulate in mussels grown on contaminated water bodies, due to the discharge of treated sewage from sewage treatment plants (STP). Despite this, some countries like Chile do not include these parasites in the control or monitoring of sewage water. The objective of this research was to evaluate the contamination of <em>C. parvum</em>. and <em>B. hominis</em> from treated sewage (disinfected by chlorination) and Cholga mussels in a touristic rural cove from the bay of Concepción. Cholga mussels from commercial stores and a treated sewage sample were analyzed. <em>Cryptosporidium</em> spp. was identified by Ziehl-Neelsen-Staining (ZNS) and <em>C. parvum</em> by direct-immunofluorescence assay (IFA) from ZNS-positive samples. <em>Blastocystis hominis</em> was identified by PCR using locus SSU rDNA. <em>C. parvum</em> and <em>B. hominis</em> subtype ST3 were found in 40% and 45% of Cholga mussel samples, respectively, and both parasites were identified in the treated sewage. <em>Blastocystis hominis</em> SSU rDNA gene alignment from Cholga mussels and treated sewage showed 89% of similarity, indicating that could be the same parasite in both samples. We describe the first evidence of possible contamination with these parasites from treated sewage to Cholga mussel suggesting an environmental contamination with high human risk. Based on these results, further studies will consider all the rural coves and STP from the bay to prevent possible contamination of these parasites.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"34 ","pages":"Article e00214"},"PeriodicalIF":2.5,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676623000276/pdfft?md5=f45512db938b8382cafd6ab22d608415&pid=1-s2.0-S2405676623000276-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138838855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiscale ecological drivers of Echinococcus multilocularis spatial distribution in wild hosts: A systematic review","authors":"Andrea Simoncini ,&nbsp;Alessandro Massolo","doi":"10.1016/j.fawpar.2023.e00216","DOIUrl":"https://doi.org/10.1016/j.fawpar.2023.e00216","url":null,"abstract":"<div><p>Understanding the ecological factors that drive the spatial patterns of parasites transmission is essential to predict their distribution under global change and to direct proactive surveillance efforts. Here, we systematically reviewed the literature to assess the main ecological drivers responsible for the spatial distribution and transmission of the zoonotic cestode <em>Echinococcus multilocularis</em>, the aetiological agent of alveolar echinococcosis, focusing on wild hosts. The 23 retrieved studies suggested that the dispersal of definitive hosts, climatic and biotic factors (distribution of intermediate hosts, composition of host communities) shape continental-scale distribution patterns of <em>E. multilocularis</em>, whereas the relative importance of climate and land cover in driving <em>E. multilocularis</em> distribution at a smaller (country/regional) scale varies with the geographic area considered. At a local scale, two additional factors contribute to determine the distribution of micro-foci of transmission: the trophic relationships between carnivores definitive hosts and small mammals intermediate hosts, and the defecation and marking behaviour of definitive hosts.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"34 ","pages":"Article e00216"},"PeriodicalIF":2.5,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S240567662300029X/pdfft?md5=87750bbf5df8889db32262cc25bcdae6&pid=1-s2.0-S240567662300029X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138501763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board Page","authors":"","doi":"10.1016/S2405-6766(23)00030-6","DOIUrl":"https://doi.org/10.1016/S2405-6766(23)00030-6","url":null,"abstract":"","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"33 ","pages":"Article e00217"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676623000306/pdfft?md5=886cd1008a099bcf14ddfc6813a4b8b0&pid=1-s2.0-S2405676623000306-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138557893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and risk factors for Taenia spp infection in pigs in Kongwa and Songwe districts, Tanzania: A cross-sectional study","authors":"Christina Wilson ,&nbsp;Robinson Hammerthon Mdegela ,&nbsp;Hezron Emmanuel Nonga ,&nbsp;George Makingi ,&nbsp;Ayubu Jacob Churi ,&nbsp;Dominik Stelzle ,&nbsp;Ernatus Martin Mkupasi ,&nbsp;Veronika Schmidt ,&nbsp;Hélène Carabin ,&nbsp;Andrea Sylvia Winkler ,&nbsp;Helena Aminiel Ngowi","doi":"10.1016/j.fawpar.2023.e00215","DOIUrl":"https://doi.org/10.1016/j.fawpar.2023.e00215","url":null,"abstract":"<div><p><em>Taenia solium</em> porcine cysticercosis (PCC) is widespread in many low- and middle-income countries (LMICs) where free-range pig rearing is common and hygienic standards are subpar. A cross-sectional survey was conducted in 42 villages between June and September 2019 (14 in Songwe district, southwest Tanzania, and 28 in Kongwa district, central Tanzania). Using a commercial Ag-ELISA kit (apDia, Belgium), circulating antigens of <em>Taenia spp</em> in pig serum were identified and used to calculate the PCC seroprevalence. The study recruited 692 randomly selected households, sampling one pig per household. The relationship between each risk factor and the seroprevalence of PCC at the household and village levels was analysed using mixed logistic regression models. The findings showed that approximately 28% of the pigs were reared in free-range settings, the proportion of households with latrines across the districts was 92%. Twenty-seven percent of households with latrines had water and soap available for hand washing. Sixty-seven (9.7%) tested positive for PCC based on Ag-ELISA. The overall seroprevalence in Kongwa and Songwe districts was 7.3% and 14.0% respectively. In addition, the overall village Ag-ELISA positivity was 9.3%, with an interquartile range (IQR) of 4.6% – 14.1%. Increasing the age of the pig (OR = 3.13 95% CI = 1.48 – 6.60; <em>p</em> = 0.003), pig originating from outside the household (OR = 0.5 95% CI = 0.25 – 0.99; <em>p</em> = 0.05), and pigs kept in a household that practised deworming (OR = 2.23 95% CI = 1.08 – 4.61; <em>p</em> = 0.03) were important risk factors associated with PCC positivity. Therefore, the high seroprevalence of PCC, up to 14%, calls for rapid and effective control actions such as vaccination and treatment of pigs against PCC, and public health education emphasises on indoor pig rearing, hygienic practices and regular use of latrines. Our findings also point to a potential danger of <em>Taenia. spp</em> infection indicating the possibility of people carrying the adult parasite <em>Taenia solium</em> not only in the rural communities of Kongwa and Songwe districts but also in the urban areas of Tanzania, where pigs from these areas are transported for consumption. To develop effective management measures, further research on taeniasis and cysticercosis in the human population is required.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"33 ","pages":"Article e00215"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676623000288/pdfft?md5=55920667d0497a0561f968db84782c70&pid=1-s2.0-S2405676623000288-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138473829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human diphyllobothriosis in Taiwan: A review of cases and molecular evidence of Dibothriocephalus nihonkaiensis","authors":"Chia-Kwung Fan ,&nbsp;Daniel Barčák ,&nbsp;Tomáš Scholz ,&nbsp;Pasaikou Sonko ,&nbsp;Martina Orosová ,&nbsp;Kua-Eyre Su ,&nbsp;Chun-Chao Chang ,&nbsp;Yuarn-Jang Lee ,&nbsp;Roman Kuchta ,&nbsp;Mikuláš Oros","doi":"10.1016/j.fawpar.2023.e00213","DOIUrl":"https://doi.org/10.1016/j.fawpar.2023.e00213","url":null,"abstract":"<div><p>Diphyllobothriosis is an infectious disease caused by the consumption of raw freshwater or marine fish containing larvae of broad tapeworms (Diphyllobothriidae). In the present study, we critically reviewed all cases of human diphyllobothriosis reported from Taiwan, including unpublished reports from hospitals in Taipei. Genotyping based on mitochondrial DNA marker (<em>cox</em>1) confirmed that two of the recent cases were caused by <em>Dibothriocephalus nihonkaiensis</em>, which is not native to Taiwan and was probably imported with Pacific salmon infected with larvae of <em>D. nihonkaiensis</em>. The causative species previously reported in Taiwan could not be definitively confirmed. However, considering the distribution of <em>Dibothriocephalus latus</em>, which is not endemic in Taiwan, past cases diagnosed as <em>D. latus</em> are questionable.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"33 ","pages":"Article e00213"},"PeriodicalIF":2.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676623000264/pdfft?md5=11ee587248019f43012d94c9ed36ad82&pid=1-s2.0-S2405676623000264-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91957317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surrogates of foodborne and waterborne protozoan parasites: A review","authors":"Laure Augendre ,&nbsp;Damien Costa ,&nbsp;Sandie Escotte-Binet ,&nbsp;Dominique Aubert ,&nbsp;Isabelle Villena ,&nbsp;Aurélien Dumètre ,&nbsp;Stéphanie La Carbona","doi":"10.1016/j.fawpar.2023.e00212","DOIUrl":"https://doi.org/10.1016/j.fawpar.2023.e00212","url":null,"abstract":"<div><p>The protozoan parasites <em>Cryptosporidium parvum</em>, <em>Cyclospora cayetanensis</em>, and <em>Toxoplasma gondii</em> are major causes of waterborne and foodborne diseases worldwide. The assessment of their removal or inactivation during water treatment and food processing remains challenging, partly because research on these parasites is hindered by various economical, ethical, methodological, and biological constraints. To address public health concerns and gain new knowledge, researchers are increasingly seeking alternatives to the use of such pathogenic parasites. Over the past few decades, several non-pathogenic microorganisms and manufactured microparticles have been evaluated as potential surrogates of waterborne and foodborne protozoan parasites. Here, we review the surrogates that have been reported for <em>C. parvum</em>, <em>C. cayetanensis</em>, and <em>T. gondii</em> oocysts, and discuss their use and relevance to assess the transport, removal, and inactivation of these parasites in food and water matrices. Biological surrogates including non-human pathogenic <em>Eimeria</em> parasites, microorganisms found in water sources (anaerobic and aerobic spore-forming bacteria, algae), and non-biological surrogates (i.e. manufactured microparticles) have been identified. We emphasize that such surrogates have to be carefully selected and implemented depending on the parasite and the targeted application. <em>Eimeria</em> oocysts appear as promising surrogates to investigate in the future the pathogenic coccidian parasites <em>C. cayetanensis</em> and <em>T. gondii</em> that are the most challenging to work with.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"33 ","pages":"Article e00212"},"PeriodicalIF":2.5,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676623000252/pdfft?md5=b5cb6902fa2600c2d7950a2fc31af632&pid=1-s2.0-S2405676623000252-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91640364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis","authors":"Lakkhana Sadaow ,&nbsp;Patcharaporn Boonroumkaew ,&nbsp;Rutchanee Rodpai ,&nbsp;Penchom Janwan ,&nbsp;Oranuch Sanpool ,&nbsp;Tongjit Thanchomnang ,&nbsp;Yasuyuki Morishima ,&nbsp;Marcello Otake Sato ,&nbsp;Yasuhito Sako ,&nbsp;Kaoru Kobayashi ,&nbsp;Misako Iwai ,&nbsp;Wanchai Maleewong ,&nbsp;Hiroshi Yamasaki ,&nbsp;Pewpan M. Intapan","doi":"10.1016/j.fawpar.2023.e00211","DOIUrl":"10.1016/j.fawpar.2023.e00211","url":null,"abstract":"<div><p>Human cysticercosis is a life-threatening zoonotic disease caused by infection with larvae (cysticerci) of the pork tapeworm, <em>Taenia solium</em>. This can affect the nervous system causing chronic headache and intracranial hypertension, potentially leading to epileptic seizures and paralysis. The disease is found in developing countries, especially in Southeast and South Asia, Sub-Saharan Africa, and Central and South America where porcine cysticercosis is endemic and people have a habit of eating undercooked pork. An immunochromatography-based test (ICT) kit, using <em>T. solium</em> cyst fluid as antigen, was manufactured to detect anti-<em>T. solium</em> IgG antibodies in human serum. To evaluate the kit, we used 187 serum samples including 24 from proven/confirmed cysticercosis cases, 133 from cases with other parasitosis and 30 healthy controls. Diagnostic efficiencies were calculated. The sensitivity, specificity, and accuracy were 83.3%, 92.0%, and 90.9%, respectively. Moreover, the ICT was positive before treatment but became negative after treatment, implying that this kit is also useful for follow-up monitoring post-treatment. In conclusion, we have successfully developed and present preliminary evaluation of an easy-to-handle rapid diagnostic tool for human cysticercosis in the form of an ICT platform using as antigen fluid from <em>T. solium</em> cysticerci.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"33 ","pages":"Article e00211"},"PeriodicalIF":2.5,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10589370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring potentially pathogenic protists in sewage sludge using Metataxonomics","authors":"Nicolas Rozo-Montoya ,&nbsp;Katherine Bedoya-Urrego ,&nbsp;Juan F. Alzate","doi":"10.1016/j.fawpar.2023.e00210","DOIUrl":"10.1016/j.fawpar.2023.e00210","url":null,"abstract":"<div><p>Intestinal parasites continue to pose a significant threat to human health worldwide, particularly among children. Contaminated water and soil serve as major transmission vehicles for these parasites and intestinal protists are among the most prevalent parasites in both developed and developing nations. Traditionally, parasites have been studied using human or animal fecal samples, while studying them in environmental samples has been challenging due to technical limitations. However, advancements in Next-Generation Sequencing (NGS) and bioinformatic approaches now enable the detection of parasite DNA in environmental samples. In this study, we applied a metataxonomic and phylogenetic strategy to detect and classify DNA of protists present in sewage sludge from two major cities in Colombia: Medellin and Cali. We successfully detected several human pathogenic parasites including <em>Giardia intestinalis</em>, <em>Entamoeba histolytica,</em> and <em>Blastocystis</em> sp., among other protists, in all sludge samples examined. We also investigated the entry and exit of parasite DNA from the San Fernando wastewater treatment plant (WWTP). We observed a higher number of parasite DNA sequences in the plant's influent wastewater, but we also detected the discharge of DNA from pathogenic parasites in both effluent waters and biosolids.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":"33 ","pages":"Article e00210"},"PeriodicalIF":2.5,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ed/88/main.PMC10558727.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41161371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}