Food and Waterborne Parasitology最新文献

{"title":"Immuno-therapeutic and prophylactic potential of Trichinella spiralis antigens for inflammatory bowel diseases","authors":"Majed H. Wakid ,&nbsp;Walaa A. El Kholy ,&nbsp;Muslimah N. Alsulami ,&nbsp;Eman S. El-Wakil","doi":"10.1016/j.fawpar.2024.e00248","DOIUrl":"10.1016/j.fawpar.2024.e00248","url":null,"abstract":"<div><div>Ulcerative colitis (UC), a severe chronic inflammatory disorder of the colon, is one of the inflammatory bowel diseases (IBD) that affects humans and several domestic animal species, including cats and dogs. Helminth infections and autoimmune diseases are inversely correlated, as explained by the hygiene hypothesis, which suggests that IBD is infrequent in countries where helminth infections are common but more prevalent in developed nations. This study investigated the therapeutic and prophylactic potential of <em>Trichinella spiralis</em> (<em>T. spiralis</em>) antigens in an experimental colitis model for IBD. Mice were divided into eight groups: normal model, colitis model, larval antigen prophylaxis, adult antigen prophylaxis, larval antigen therapeutic, adult antigen therapeutic, larval antigen prophylaxis and therapeutic, and adult antigen prophylaxis and therapeutic. Colitis was induced intrarectally by administering a single dose of 0.2 ml of acetic acid, except in the healthy group, which received PBS (0.2 ml). The mice were euthanized 12 days after colitis induction. The therapeutic and prophylactic potential of <em>T. spiralis</em> antigens were assessed through colitis severity and histopathological, immunological, and immunohistochemical examinations. The results showed a significant reduction in Disease Activity Index (DAI), an increase in goblet cells' acidic mucin levels, reduced iNOS and TNF-α expression, and decreased serum levels of IFN-γ and IL-10 cytokines in Groups IV-VIII compared to the colitis model, particularly in the group that received adult worm antigen both prophylactically and therapeutically. This study demonstrated that <em>T. spiralis</em> antigens, especially from adult worms, had protective and therapeutic effects on experimental colitis, with a superior effect when administered both before and after colitis induction by reducing inflammation and modulating the immune response. Thus, <em>T. spiralis</em> antigens may improve disease outcomes and provide a novel treatment approach for ulcerative colitis.</div></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auditing of Danish pig herds for controlled housing requirements and Trichinella","authors":"Lis Alban ,&nbsp;Heidi Enemark ,&nbsp;Heidi Huus Petersen ,&nbsp;Lisbeth Harm Nielsen","doi":"10.1016/j.fawpar.2024.e00247","DOIUrl":"10.1016/j.fawpar.2024.e00247","url":null,"abstract":"<div><div><em>Trichinella</em> is a zoonotic parasite, which historically has been of large concern for public health in Europe. Consequently, testing of all pigs for <em>Trichinella</em> has been mandatory in many European countries, even though <em>Trichinella</em> is almost exclusively found in outdoor or backyard production. The idea therefore emerged that auditing for biosecurity should replace testing in indoor production. In the European Union (EU), pigs raised under so-called controlled housing conditions are exempt from testing. The specific requirements for a pig production to be considered a controlled housing holding are described in Annex IV in the EU <em>Trichinella</em> Regulation No. 2015/1375. In Denmark, the controlled housing concept is used extensively, not to omit <em>Trichinella</em> testing but to allow visual-only post-mortem inspection at export-authorized abattoirs. The Danish pig industry has established a quality assurance scheme called DANISH Product Standard, which is used to assure that the pig production maintains specific standards. This paper describes how the control, including the auditing, is set up and shows how the EU requirements regarding controlled housing are controlled. Moreover, the EU requirements are compared with the recommendations issued by the International Commission on Trichinellosis and the World Organisation for Animal Health. Finally, strengths, weaknesses, opportunities and threats of the Danish way of documenting indoor finisher herds' compliance with controlled housing as specified by the EU are discussed. It is concluded that the validity of the system is high.</div></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An immunochromatographic test using whole blood for rapid diagnosis of human paragonimiasis and its diagnostic usefulness","authors":"Patcharaporn Boonroumkaew ,&nbsp;Lakkhana Sadaow ,&nbsp;Penchom Janwan ,&nbsp;Rutchanee Rodpai ,&nbsp;Oranuch Sanpool ,&nbsp;Tongjit Thanchomnang ,&nbsp;Hiroshi Yamasaki ,&nbsp;Pewpan M. Intapan ,&nbsp;Wanchai Maleewong","doi":"10.1016/j.fawpar.2024.e00246","DOIUrl":"10.1016/j.fawpar.2024.e00246","url":null,"abstract":"<div><div>Paragonimiasis is a harmful food-borne zoonosis caused by lung flukes of the genus <em>Paragonimus</em>. The disease is found on most continents, several million people are at risk of infection, and it is a re-emerging disease in developing countries. The gold standard for diagnosis of pulmonary paragonimiasis requires the finding of eggs in sputa and/or fecal samples. In ectopic paragonimiasis cases, eggs are typically not seen, and supportive information is required such as a history of eating freshwater crabs or crayfishes, radiographic findings and immunological tests. Here, we developed a proof of concept based on lateral flow assay, an immunochromatographic test kit, named the paragonimiasis whole-blood test kit, for detection of specific IgG antibody in simulated whole-blood samples (WBSs) using worm excretory-secretory antigens to diagnose human paragonimiasis. The laboratory diagnostic values of this kit were compared with the detected IgG in serum samples. In simulated WBSs, the diagnostic sensitivity and specificity were 97.8 % and 96.1 %, respectively, while for serum samples, these values were 100.0 % and 94.8 %, respectively. The comparative IgG antibody detections whether a result was positive or negative between simulated WBSs and serum samples did not differ significantly with a concordance of 97.8 % in laboratory conditions using a circumscribed set of samples. The tool is fast and easy to use. The next step involves observing and evaluating native whole blood samples and using specific recombinant antigens need to be evaluated for support diagnosis of paragonimiasis caused by <em>P. heterotremus, P. westermani</em> and <em>P. miyazakii</em> at the bedside or at local and remote hospitals with limited facilities. It will also be valuable for epidemiological surveys in Asia where paragonimiasis is endemic.</div></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board Page","authors":"","doi":"10.1016/S2405-6766(24)00026-X","DOIUrl":"10.1016/S2405-6766(24)00026-X","url":null,"abstract":"","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S240567662400026X/pdfft?md5=b4424fee49115d3321686e34a0ab0fb1&pid=1-s2.0-S240567662400026X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filters comprised of sand and Zero Valent Iron hold promise as tools to mitigate risk posed by Cyclospora cayetanensis oocysts","authors":"C. Yeager ,&nbsp;M. Tucker ,&nbsp;A. Gutierrez ,&nbsp;C. O'Brien ,&nbsp;M. Sharma ,&nbsp;V. Fournet ,&nbsp;J.P. Dubey ,&nbsp;M. Jenkins ,&nbsp;K. Kniel ,&nbsp;B.M. Rosenthal","doi":"10.1016/j.fawpar.2024.e00243","DOIUrl":"10.1016/j.fawpar.2024.e00243","url":null,"abstract":"<div><p>Irrigation water contaminated by human fecal material may elevate the risk of produce contamination with the enteric parasite <em>Cyclospora cayetanensis.</em> Oocysts of <em>C. cayetanensis</em> are resistant to commonly used disinfectants and a method of removing <em>C. cayetanensis</em> from irrigation water would mitigate this risk. We evaluated zero valent iron (ZVI) sand filtration as one such method. We sought to determine if sand filters containing ZVI outperformed those without ZVI. We first evaluated the abundant poultry parasites <em>Eimeria maxima</em>, <em>E. tenella</em> and <em>E. acervulina</em> as surrogates for <em>C. cayetanensis</em>. We determined if a miniaturized gravity fed ZVI-sand filter, scaled to evaluate scarce supplies of <em>C. cayetanensis</em> oocysts, provided useful information about the performance of larger filtration systems. Filters were inoculated with oocysts, rinsed, and the resulting filtrate examined microscopically for oocysts. We performed experiments to measure the effect of varying ZVI concentrations, repeated filter use, simulated agricultural water, and oocyst size and condition. We then compared the performance of mini filters to that of larger, gravity-fed pool filters and found that ZVI-sand filtration was far more effective at removing <em>Eimeria</em> spp. from water when compared to sand filtration, at both scales. Sand mini filters retained 13–54 % of <em>E. acervulina</em> oocysts, and pool filters retained 82 %, but when combined with 50 % (mini filter) or 35 % (pool filter) <em>v</em>/v ZVI, mini filters retained 89–99 % of oocysts and pool filters retained &gt;99 %. The effectiveness of the mini filters increased with increasing ZVI concentration, and the addition of ZVI far outweighed the influence of any other measured variable. We then performed experiments including <em>C. cayetanensis</em>, which provided similar results to those utilizing <em>Eimeria</em>; 59 % of inoculated <em>C. cayetanensis</em> oocysts were retained in sand mini filters, and 97 % in mini filters composed of 35 % <em>v</em>/v ZVI. In sum, ZVI is highly effective in removing oocysts from water and <em>Eimeria</em> is a useful surrogate for <em>C. cayetanensis</em> to assess filtration. ZVI-sand filtration shows promise as a tool to mitigate the risk of <em>C. cayetanensis</em> contamination of irrigation water. Further studies should evaluate the performance of ZVI-sand in pressurized fast filtration systems under a range of field conditions.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000258/pdfft?md5=bbf171f834dbc70de72425db565754f5&pid=1-s2.0-S2405676624000258-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142151545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foodborne concerns of Blastocystis spp. in marine animals (fish, bivalves, and sponges): A systematic review and meta-analysis of global prevalence and subtypes distribution","authors":"Mohammad Ghafari-Cherati ,&nbsp;Amin Karampour ,&nbsp;Seyedeh-Sara Nazem-Sadati ,&nbsp;Ali Asghari","doi":"10.1016/j.fawpar.2024.e00242","DOIUrl":"10.1016/j.fawpar.2024.e00242","url":null,"abstract":"<div><p><em>Blastocystis</em> spp. is a common intestinal parasite found in humans and various animals, including marine species like fish, bivalves, and sponges. While traditionally considered non-pathogenic, emerging evidence suggests potential foodborne concerns, especially for vulnerable populations. The present systematic review and meta-analysis reviewed four electronic databases (PubMed, Scopus, Google Scholar, and Web of Science) until June 13, 2024, for studies reporting the prevalence and subtypes (STs) distribution of <em>Blastocystis</em> spp. in marine animals, including fish, bivalves, and sponges, to assess foodborne concern and zoonotic importance. In the analysis of 11 studies involving 1329 marine animals from nine countries, five studies/datasets (742 samples) focused on fish, five studies/datasets (567 samples) on bivalves, and one (20 samples) on sponges. This review found that 12.4 % (95 % CI: 4.3–31 %) of marine animals globally were infected by <em>Blastocystis</em> spp., with bivalves showing the highest infection rate at 32 % (95 % CI: 13–59.7 %), exceeding sponges with a single study at 10 % (95 % CI: 2.5–32.4 %), and fish at 4.4 % (95 % CI: 2–9.3 %). Sensitivity analysis assessed changes in weighted prevalence after excluding certain studies. A subgroup analysis of <em>Blastocystis</em> spp. prevalence was conducted based on publication years, countries, continents, WHO regions, and sample sizes. The data collected indicated that marine animals serve as suitable reservoirs for various <em>Blastocystis</em> spp. STs (ST1-ST4, ST7, ST8, ST10, ST14, ST23, ST26, and ST44), with most (except for ST26 and ST44) having the potential for zoonotic transmission. Overall, the findings emphasize the potential for foodborne risk posed by <em>Blastocystis</em> spp. in marine animals and highlight the need for improved monitoring and control measures to ensure food safety.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000246/pdfft?md5=3a2f1a00dcec4ef666712825e4386312&pid=1-s2.0-S2405676624000246-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Entamoeba histolytica/dispar and Giardia lamblia infections and their associated factors among schoolchildren in the Amhara region, Northwest Ethiopia","authors":"Tadesse Hailu,&nbsp;Getaneh Alemu","doi":"10.1016/j.fawpar.2024.e00241","DOIUrl":"10.1016/j.fawpar.2024.e00241","url":null,"abstract":"<div><p>Globally, intestinal protozoa <em>E. histolytica/dispar</em> and <em>Giardia lamblia</em> are the cause of amoebiasis and giardiasis, respectively. Despite their important medical importance and common occurrence in Ethiopia, they are minimally addressed in terms of their prevalence, sensitive diagnostic methods, and associated risk factors. Infections with <em>E. histolytica/dispar</em> and <em>G. lamblia</em> are often misdiagnosed and underreported in impoverished countries. Thus, the purpose of this study was to ascertain the prevalence of <em>Giardia lamblia</em> and <em>E. histolytica/dispar</em> infections as well as related variables among schoolchildren in the Amhara region. A cross-sectional study was conducted among 844 schoolchildren in the Amhara region from April to December 2019. A stool sample was collected from each study participant and processed via the formol ether concentration technique (FECT) and spontaneous tube sedimentation techniques (STST). Data were entered in EpiData and analysed by SPSS statistical software. The prevalence of <em>E. histolytica</em> and <em>G. lamblia</em> infections using each diagnostic method and composite reference was determined by descriptive statistics. The association of risk factors with <em>E. histolytica/dispar</em> and <em>G. lamblia</em> infections was analysed by logistic regression and variables with <em>p</em> &lt; 0.05 were considered to have statistical significance. From the total, 243 (28.8%) schoolchildren were found to be infected by at least one of <em>E. histolytica/dispar</em> or <em>G. lamblia</em>. The prevalence of <em>E. histolytica/dispar</em> and <em>G. lamblia</em> infections was 201 (23.8%) and 62 (7.3%), respectively. The co-infection prevalence with both <em>E. histolytica/dispar</em> and <em>G. lamblia</em> was 22 (2.6%). The sensitivity (78.6%) and negative predictive value of STST (19.6%) were higher than FECT sensitivity (65.4%) and negative predictive value (13.1%). Children in 10–14 years of age (AOR = 1.66;95%CI: 1.16–2.38), lived in the rural (AOR = 1.97;95%CI: 1.12–3.49), used latrine improperly (AOR = 1.49;95%CI: 1.04–2.13), did not wash hands before meal (AOR = 2.10; 95%CI:1.08–4.10), and after latrine (AOR = 1.51;95%CI: 1.05–2.19), ate unwashed raw vegetables (AOR = 1.85;95%CI:1.26–2.70), and played with soil (AOR = 1.48;95%CI:1.06–2.06) were associated with <em>E. histolytica/dispar</em> and <em>G. lamblia</em> infection. These findings revealed high prevalence of <em>E. histolytica/dispar</em> and <em>G. lamblia</em> infections was high in the Amhara region. Therefore, proper implementation of water, sanitation and hygiene should be advocated at the community and school levels to mitigate the disease burden.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000234/pdfft?md5=e2583e7a393b915173c94a01047a3ff2&pid=1-s2.0-S2405676624000234-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evidence of human exposure to foodborne zoonotic parasites in Romanian patients and possible association with food habits and hygiene","authors":"Violeta Briciu ,&nbsp;Zsuzsa Kalmár ,&nbsp;Anca Ieremia ,&nbsp;Mihaela Lupșe ,&nbsp;Mirela Flonta ,&nbsp;Monica Muntean ,&nbsp;Cristina Cismaru ,&nbsp;Melinda Horvat ,&nbsp;Amanda Rădulescu ,&nbsp;Adriana Topan ,&nbsp;Cristian Jianu ,&nbsp;Angela Monica Ionică","doi":"10.1016/j.fawpar.2024.e00240","DOIUrl":"10.1016/j.fawpar.2024.e00240","url":null,"abstract":"<div><p>Humans may become accidental dead-end hosts for a series of zoonotic foodborne parasites, of which <em>Toxoplasma gondii</em>, <em>Echinococcus</em> spp., <em>Toxocara</em> spp., and <em>Trichinella spiralis</em> are of major public health interest, due to their potential pathological implications. The aims of the study were to evaluate the exposure to these pathogens in north-western Romania, and to investigate their potential association to risk factors. From June 2022 to January 2024, 554 patients admitted to a tertiary hospital in north-western Romania were screened for the presence of IgG antibodies against <em>T. gondii</em>, <em>Echinococcus</em> spp., <em>Toxocara canis</em>, and <em>T. spiralis</em> by ELISA, and potential risks were assessed using a questionnaire. Overall, 225 samples (40.6%) were positive for at least one pathogen. The highest seroprevalence for IgG was found for <em>T. gondii</em> (33.9%), followed by <em>Echinococcus</em> spp. (9.1%), <em>T. spiralis</em> (2.9%)<em>,</em> and <em>T. canis</em> (1.1%). For <em>T. spiralis</em>, raw meat consumption was associated with positivity. For <em>T. gondii,</em> increased age, rural environment, contact with cats, consumption of unwashed fruits/vegetables and drinking water from unverified sources were significantly associated to seropositivity. The present study provides new insights into the epidemiological status of zoonotic foodborne parasite in Romania, underlining the need to increase awareness on the importance of water, sanitation and food habits in relation with this neglected pathology.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000222/pdfft?md5=a166f282445366862aca9e71ada58866&pid=1-s2.0-S2405676624000222-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over a century of progress on Trichinella research in pigs at the United States Department of Agriculture: Challenges and solutions","authors":"Jitender P. Dubey,&nbsp;Peter C. Thompson,&nbsp;Valsin Fournet,&nbsp;Dolores E. Hill ,&nbsp;Dante Zarlenga ,&nbsp;H. Ray Gamble ,&nbsp;Benjamin M. Rosenthal","doi":"10.1016/j.fawpar.2024.e00239","DOIUrl":"10.1016/j.fawpar.2024.e00239","url":null,"abstract":"<div><p>Trichinellosis, caused by 13 species/subspecies/genotypes in the nematode genus <em>Trichinella</em>, is a worldwide zoonosis. In the United States, trichinellosis was of historical and economic significance because of European restrictions on the import of U.S. pork. Before the advent of effective protective measures, most cases of trichinellosis were derived from consumption of undercooked or inadequately processed, infected pork. Research conducted at the United States Department of Agriculture (USDA) since 1891, and policies established by USDA regulatory agencies, have helped to reduce <em>Trichinella</em> infections in commercially raised domestic pigs to negligible levels. Here, we review the history of this scientific progress, placing special emphasis on research conducted at the USDA's Beltsville Agricultural Research Center.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000210/pdfft?md5=dcf75dffc252930512b6ae9786b10464&pid=1-s2.0-S2405676624000210-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability of Metagonimus romanicus (Ciurea, 1915) metacercariae after physico-chemical treatments","authors":"Martina Gyöngy ,&nbsp;Boglárka Sellyei ,&nbsp;István Czeglédi ,&nbsp;Csaba Székely ,&nbsp;Gábor Cech","doi":"10.1016/j.fawpar.2024.e00236","DOIUrl":"10.1016/j.fawpar.2024.e00236","url":null,"abstract":"<div><p>Digenean (Heterophyidae) trematodes include several zoonotic species such as the genus <em>Metagonimus</em> (Katsurada, 1912). <em>Metagonimus romanicus</em> (Ciurea, 1915) is a species widely distributed in Europe, whose metacercariae can be found on cyprinoids from the River Danube in Hungary. The aim of the study was to measure the viability of <em>Metagonimus romanicus</em> metacercariae by physical (freezing, heating, desiccation) and chemical (acetic acid and NaCl solutions) treatments. The methods were chosen as a model for procedures commonly used in traditional food preservation (such as freezing, salting, pickling and smoking) to measure the survival rate of metacercariae under different conditions. Most physical treatments (freezing of metacercariae at −20 °C, keeping them at 40 °C and 60 °C and desiccation) and chemical treatments (2.5%, 5%, 10% acetic acid solution and 5% and 10% NaCl solution) killed the metacercariae in a relatively short time (from 30 min to 6 days depending on the treatment) so their effects eliminated the risk of zoonotic infection. On the other hand, the metacercariae survived at room temperature and at 4 °C up to one month. Therefore, storing unprepared fish in domestic refrigerators cannot prevent infection with metacercariae in humans.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000180/pdfft?md5=67789140c9cdfa84f3204f48020323da&pid=1-s2.0-S2405676624000180-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}