Food and Waterborne Parasitology最新文献

{"title":"Fasciola hepatica: Updates on egg morphology, host range, and distribution","authors":"Olga Loginova ,&nbsp;Boris Efeykin ,&nbsp;Anna Krutikova ,&nbsp;Ivan Mizin ,&nbsp;Sergei Spiridonov","doi":"10.1016/j.fawpar.2024.e00237","DOIUrl":"10.1016/j.fawpar.2024.e00237","url":null,"abstract":"<div><p>During a survey for helminths in reindeer (<em>Rangifer tarandus</em>) across the Palearctic region, eggs were found in zoo reindeer feces. These were identified as eggs of <em>Fasciola hepatica</em> based on their morphology, morphometrics, location, and analysis of their partial sequence of ITS rDNA region. Some of the eggs had an appendage, previously unreported. Additionally, adults of <em>F. hepatica</em> were studied. Eggs derived from their uteri were also appendaged. Diagnostic morphological traits of <em>F. hepatica</em> eggs (abopercular appendage, knob, egg shell thickening, and egg shape) are discussed in this article. Three dimensional models of <em>F. hepatica</em> eggs were created to demonstrate the eggs features as best as possible. Since fecal examination remains gold standard in diagnosing fasciolosis in humans and animals worldwide, our findings may contribute to improved diagnostics. This research has also shown that reindeer can be a final host for <em>F. hepatica</em>. We also discuss whether the Novaya Zemlya archipelago might be the northernmost site of fasciolosis.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000192/pdfft?md5=23fa16089f7f240a103dff16c2eacf45&pid=1-s2.0-S2405676624000192-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance for Trichinella infection in U.S. pigs raised under controlled management documents negligible risk for public health","authors":"H. Ray Gamble ,&nbsp;Dolores E. Hill ,&nbsp;Valsin Fournet ,&nbsp;Brandon Adams ,&nbsp;Diane Hawkins-Cooper ,&nbsp;Jorrell Fredericks ,&nbsp;Jovan Aquino ,&nbsp;Sonia Agu ,&nbsp;Nadya Chehab ,&nbsp;Ako Ankrah ,&nbsp;Maria C. Antognoli ,&nbsp;Marta D. Remmenga ,&nbsp;Scott Kramer ,&nbsp;Lori Gustafson ,&nbsp;Benjamin M. Rosenthal","doi":"10.1016/j.fawpar.2024.e00238","DOIUrl":"10.1016/j.fawpar.2024.e00238","url":null,"abstract":"<div><p>Biosecurity measures preventing exposure of pigs to rodents, wildlife, and contaminated feed or waste products reduce the risk of zoonotic <em>Trichinella</em> infection in pork. To understand the benefits of such measures in the United States, we conducted the first comprehensive survey of pigs produced under the Pork Quality Assurance Plus production standard, surveying 3,208,643 pork samples from twelve processing locations tested over a period of 54 months. We detected no <em>Trichinella</em> sp. positives in any of these pork samples, providing a 95% confidence in a <em>Trichinella</em> sp. prevalence of &lt;1 in 1,000,000 for the processors represented by the study. These results are consistent with international guidelines for having a negligible risk to public health. Results obtained here should generalize to all PQA+ sources, as <em>Trichinella</em> sp. exposure risk is based on production guidelines that extend to the larger PQA+ population.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000209/pdfft?md5=9cb74fd6ad79768ab8b5d3e6f69d544d&pid=1-s2.0-S2405676624000209-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global epidemiology and species/genotype distribution of Cryptosporidium in camels: A systematic review and meta-analysis","authors":"Farzad Mahdavi ,&nbsp;Farajolah Maleki ,&nbsp;Mohammad Reza Mohammadi ,&nbsp;Ali Asghari ,&nbsp;Behnam Mohammadi-Ghalehbin","doi":"10.1016/j.fawpar.2024.e00235","DOIUrl":"10.1016/j.fawpar.2024.e00235","url":null,"abstract":"<div><p>This review analyzed reported data of <em>Cryptosporidium</em> prevalence in camels and the species/genotype distribution. Four databases (PubMed, Web of Science, Scopus, Google Scholar) were screened, and studies published by April 1, 2024, were included. Total estimates and 95% CIs were calculated using a random-effects model. The weighted prevalence of <em>Cryptosporidium</em> spp. in 7372 camels examined from 12 different countries was estimated at 13.8% with a 95% CI of 10.3–18.4%. The sensitivity analysis based on excluding the individual studies did not result in significant statistical changes in the final weighted prevalence. Subgroup prevalence of <em>Cryptosporidium</em> spp. in camels was analyzed by publication year, continent, WHO region, country, camel type, sample size, diagnostic method, age, and gender. A significant publication bias (<em>P</em> &lt; 0.05) was reported in the present study. Limitations encountered in this study encompassed: insufficient study diversity, reliance on single study results, inadequate molecular and serological studies in comparison to microscopic studies, etc., all of which could impact the findings. The study identified eight <em>Cryptosporidium</em> spp. in camels: <em>C. parvum</em>, <em>C. andersoni</em>, <em>C. bovis</em>, <em>C. muris</em>, <em>C. ratti</em>, <em>C. occultus</em>, <em>C. ubiquitum</em>, and <em>C. hominis</em>. The first three species had pooled prevalence rates of 65.5%, 66%, and 19.2%, respectively. Each of the remaining five species was documented using a single dataset/study. Moreover, genotypes IIdA19G1, IIaA15G1R1, If-like-A15G2, IIdA15G1, IIaA15G2R1, IIaA17G2R1, and IIaA18G2R1 (<em>C. parvum</em>), genotype IV (<em>C. ratti</em>), genotype XIIa (<em>C. ubiquitum</em>), and genotype IkA19G1 (<em>C. hominis</em>) have been identified in camels globally. The findings suggest that camels can act as a source of infection for a variety of <em>Cryptosporidium</em> species/genotypes, and can therefore play a key role in disseminating this protozoan to humans and animals.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000179/pdfft?md5=3c3128cfd2d1685a338f2627f1f3cf00&pid=1-s2.0-S2405676624000179-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Taenia solium cysticercosis in domestic pigs following albendazole deworming intervention in rural communities of Mbulu district, Tanzania","authors":"Vedasto Bandi ,&nbsp;Bernard Ngowi ,&nbsp;Emmanuel Mpolya ,&nbsp;Andrew Martin Kilale ,&nbsp;John-Mary Vianney","doi":"10.1016/j.fawpar.2024.e00234","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00234","url":null,"abstract":"<div><p>Taeniosis and cysticercosis are infections caused by cestodes, <em>Taenia solium</em> is among them. <em>T.solium</em> neurocysticercosis accounts for 30% of acquired epilepsy in human in developing countries. This study was carried out to determine the prevalence of cysticercosis among domestic pigs in Mbulu district following deworming intervention. The study was conducted among three rural communities monitoring community intervention in Mbulu district between March 2020 and September 2021. Live pigs were diagnosed by lingual examination for the presence of <em>T. solium</em> cysticerci<em>,</em> and pig-rearing practices were recorded. Logistic regression was performed to determine the role of risk factors on pig infection outcome. We conveniently sampled 510 pigs; 267 (52.4%) were sampled in the year 2020 and 243 (47.6%) in 2021. All pigs were examined by lingual examination for the presence of pork tapeworm larvae, and 43 (8.4%) pigs were found to be infected. Twenty-one (48.8%) of the infected pigs were males and 22 (51.2%) were females, and the overall annual prevalence of tapeworm larvae was 9% and 7.8% for 2020 and 2021, respectively. The pigs were twice more likely to be found infected during the rainy season compared to the dry season in 2020 (OR = 2.27, 95%CI of 1.16–7.22). The reported pig-rearing practices were free-range, penned, and tethered, 141 (52.8%), 64 (24%), and 62 (23.2%), respectively. Of the 94 visited households in 2020, 78 (83%) reported drinking water without boiling, and 59 (62.8%) household leaders reported having heard about taeniosis/cysticercosis. The prevalence of cysticercosis among domestic pigs in this study was high, with seasonal variations. Despite the ongoing national school deworming and community deworming program, there was no significant change in the prevalence of cysticercosis over two consecutive years. The reported pig infections imply fecal-oral transmission with humans tapeworm eggs released from infected humans. Most households reported consuming unboiled drinking water that might be contaminated. Integrating pig vaccination and deworming, health education and school or community deworming along with improved pig management practice and general community water sanitation hygiene (WASH) are recommended to reduce the burden of pork tapeworm in the study communities.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000167/pdfft?md5=4341a2380b18df491579e2ddc1a51678&pid=1-s2.0-S2405676624000167-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141605527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board Page","authors":"","doi":"10.1016/S2405-6766(24)00013-1","DOIUrl":"https://doi.org/10.1016/S2405-6766(24)00013-1","url":null,"abstract":"","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000131/pdfft?md5=36f74cbcc23eb757a7d52f94cb60bf7a&pid=1-s2.0-S2405676624000131-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141429343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the genetic diversity of Angiostrongylus spp. causing human angiostrongyliasis and implications for molecular identification and diagnosis","authors":"Abigail Hui En Chan,&nbsp;Chanisara Kaenkaew,&nbsp;Wallop Pakdee,&nbsp;Urusa Thaenkham","doi":"10.1016/j.fawpar.2024.e00230","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00230","url":null,"abstract":"<div><p><em>Angiostrongylus cantonensis</em> and <em>Angiostrongylus costaricensis</em> are known human pathogens responsible for eosinophilic angiostrongyliasis and abdominal angiostrongyliasis, respectively. Humans are accidental hosts, where infection occurs through the consumption of the infective larva stage 3 in intermediate or paratenic hosts. The proven method for abdominal angiostrongyliasis diagnosis is the histological examination through tissue biopsy, while the diagnosis of eosinophilic angiostrongyliasis is the detection of larva in the cerebrospinal fluid. As there is molecular evidence of cryptic species within <em>A. cantonensis</em> and <em>A. costaricensis</em> lineages, along with morphological similarities within both lineages, accurate species identification and disease diagnosis may be challenging. Moreover, species within the lineages share similar intermediate and definitive hosts and geographic distribution. For example, both <em>A. cantonensis</em> and <em>Angiostrongylus malaysiensis</em> (a closely related species in <em>A. cantonensis</em> lineage) overlap in their geographic distribution in Southeast Asia. Additionally, variations in the molecular makeup of <em>A. costaricensis</em> and <em>A. cantonensis</em> lineages may impact the pathogenicity, infectivity, and disease severity of angiostrongyliasis. Understanding of the genetic diversity of both lineages is a cornerstone for improved diagnosis and disease intervention, especially in a changing global environment. To shed light and provide insights into the genetic diversity of the <em>Angiostrongylus</em> lineages causing human angiostrongyliasis, we aim to present an up-to-date review of the studies conducted and genetic markers used for <em>A. costaricensis</em> and <em>A. cantonensis</em> lineages. The implications for accurate molecular identification and diagnosis of human angiostrongyliasis are also discussed.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S240567662400012X/pdfft?md5=1f3438e2f587cb4a8733a8f9810a2dde&pid=1-s2.0-S240567662400012X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141090184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Gnathostoma spinigerum larva in the brain with complete follow-up after surgical treatment of human neurognathostomiasis","authors":"Chayanuchit Chayangsu ,&nbsp;Sumate Ampawong ,&nbsp;Onrapak Reamtong ,&nbsp;Parnpen Viriyavejakul ,&nbsp;Tapanee Kanjanapruthipong ,&nbsp;Kamonpan Fongsodsri ,&nbsp;Suwaphat Intapun ,&nbsp;Pongwat Polpong ,&nbsp;Rattanarat Intarat ,&nbsp;Prakaykaew Charunwatthana ,&nbsp;Abigail Hui En Chan ,&nbsp;Dorn Watthanakulpanich","doi":"10.1016/j.fawpar.2024.e00229","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00229","url":null,"abstract":"<div><p>Human gnathostomiasis is a food-borne zoonotic helminthic infection widely reported in Latin America, Asia, and Southeast Asia. Consuming raw, or under-cooked fresh-water fish is the leading cause of this helminthic infection, which is clinically characterized by signs of inflammation, itching sensation, or irritation with migratory swelling. Neurological symptoms resulting from neurognathostomiasis vary, and there is scant information due to the rareness of patient brain samples. This study aimed to demonstrate the first evidence of human neurognathostomiasis by the detection of <em>Gnathostoma spinigerum</em> larva in patient's brain during craniotomy, supported by histopathological, immunological and proteomic evidence. Clinical symptoms were obtained from medical history and physical examination with laboratory investigations, including magnetic resonance imaging (MRI), left temporal craniotomy, histopathology of brain tissue, and Western blot analysis, were performed to elucidate the causative pathogens for diagnosis. In addition, the host–parasite interaction of the parasite invading the patient's brain was characterized through proteomics. Histopathology revealed worms with the characteristic cuticular spines of <em>G. spinigerum</em> which were detected and identified. These histopathological findings were consistent with a positive Western blot showing a 24-kDa reactive-band for gnathostomiasis. Proteomic analysis revealed the presence of <em>G. spinigerum</em> serpin and serine protease in the patient's serum. Moreover, the leucine-rich alpha-2-glycoprotein was indicated as a systemic biomarker of early brain injury related to invasion by <em>G. spinigerum.</em> Therefore, our study provides the initial evidence of human neurognathostomiasis due to <em>G. spinigerum</em> larval invasion along with successful craniotomy and proven larval detection including complete follow-up, and the disease prognosis after surgical treatment.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000118/pdfft?md5=fcf7652d053b0e92153302481a3da542&pid=1-s2.0-S2405676624000118-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140947155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and evaluation of loop-mediated isothermal amplification for rapid detection of Enterocytozoon bieneusi","authors":"Fatemeh Mahdavi ,&nbsp;Hamed Mirjalali ,&nbsp;Maryam Niyyati ,&nbsp;Seyyed Javad Seyyed Tabaei ,&nbsp;Amir Shamloo ,&nbsp;Hamid Asadzadeh Aghdaei","doi":"10.1016/j.fawpar.2024.e00225","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00225","url":null,"abstract":"<div><p><em>Enterocytozoon bieneusi</em> is one of the most prevalent microsporidia species, responsible for more than 90% of human and animal microsporidiosis. Microsporidia species, particularly <em>E. bieneusi,</em> are frequently reported from waterborne and foodborne outbreaks. Therefore, early detection is crucial in clinics and outbreak investigations. This study aimed to design a loop-mediated isothermal amplification (LAMP) for rapid detection of <em>E. bieneusi.</em> Total DNA was extracted from 30 <em>E. bieneusi</em> –positive samples, which had been confirmed with nested PCR. LAMP primers were designed based on the identical fragment of small subunit ribosomal RNA (<em>SSU rRNA</em>) gene. LAMP reactions were performed at 63 °C for 60 min. The sensitivity and specificity of the assay were analyzed and the results of amplification were compared to real-time PCR. Our results showed that the LAMP assay successfully amplified 25/30 (83.3%) samples. The specificity results indicated no false positive with other microorganisms. Furthermore, the LAMP method exhibited a sensitivity (limit of detection, LoD) as low as 34 ag/μL of total DNA. Compared to the LAMP assay, real-time PCR was able to detect all 30 nested PCR-positive samples. Our findings showed that the LAMP assay was able to detect 83.3% of <em>E. bieneusi-</em>positive samples. Although the current assay was not able to detect all nested PCR-positive samples, the lack of need for specific instruments, rapid processes, and high specificity makes LAMP assay a suitable tool for screening.</p></div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000076/pdfft?md5=b0e8a221af6d8b2c0a4de899ce0e7bc5&pid=1-s2.0-S2405676624000076-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140138187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Molecular detection of Toxoplasma gondii in chicken hearts from markets and retail stores in Northern Iran” [Food and Waterborne Parasitology 27 (2022) e00166]","authors":"Somayeh Abbaszadeh ,&nbsp;Aref Teimouri ,&nbsp;Mohammad Reza Mahmoudi ,&nbsp;Zahra Atrkar Roushan ,&nbsp;Nayereh Hajipour ,&nbsp;Bijan Majidi-Shad ,&nbsp;Meysam Sharifdini","doi":"10.1016/j.fawpar.2024.e00224","DOIUrl":"https://doi.org/10.1016/j.fawpar.2024.e00224","url":null,"abstract":"","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000064/pdfft?md5=4fb337f831164bf099df8ad4ca97c8cc&pid=1-s2.0-S2405676624000064-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140137957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board Page","authors":"","doi":"10.1016/S2405-6766(24)00008-8","DOIUrl":"https://doi.org/10.1016/S2405-6766(24)00008-8","url":null,"abstract":"","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405676624000088/pdfft?md5=37948a8b48e7dcb4be9b8bb01f9ae944&pid=1-s2.0-S2405676624000088-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140137956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}