Immuno-therapeutic and prophylactic potential of Trichinella spiralis antigens for inflammatory bowel diseases

IF 2.9 Q2 PARASITOLOGY

Food and Waterborne Parasitology Pub Date : 2024-10-06 DOI:10.1016/j.fawpar.2024.e00248

Majed H. Wakid , Walaa A. El Kholy , Muslimah N. Alsulami , Eman S. El-Wakil

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引用次数: 0

Abstract

Ulcerative colitis (UC), a severe chronic inflammatory disorder of the colon, is one of the inflammatory bowel diseases (IBD) that affects humans and several domestic animal species, including cats and dogs. Helminth infections and autoimmune diseases are inversely correlated, as explained by the hygiene hypothesis, which suggests that IBD is infrequent in countries where helminth infections are common but more prevalent in developed nations. This study investigated the therapeutic and prophylactic potential of Trichinella spiralis (T. spiralis) antigens in an experimental colitis model for IBD. Mice were divided into eight groups: normal model, colitis model, larval antigen prophylaxis, adult antigen prophylaxis, larval antigen therapeutic, adult antigen therapeutic, larval antigen prophylaxis and therapeutic, and adult antigen prophylaxis and therapeutic. Colitis was induced intrarectally by administering a single dose of 0.2 ml of acetic acid, except in the healthy group, which received PBS (0.2 ml). The mice were euthanized 12 days after colitis induction. The therapeutic and prophylactic potential of T. spiralis antigens were assessed through colitis severity and histopathological, immunological, and immunohistochemical examinations. The results showed a significant reduction in Disease Activity Index (DAI), an increase in goblet cells' acidic mucin levels, reduced iNOS and TNF-α expression, and decreased serum levels of IFN-γ and IL-10 cytokines in Groups IV-VIII compared to the colitis model, particularly in the group that received adult worm antigen both prophylactically and therapeutically. This study demonstrated that T. spiralis antigens, especially from adult worms, had protective and therapeutic effects on experimental colitis, with a superior effect when administered both before and after colitis induction by reducing inflammation and modulating the immune response. Thus, T. spiralis antigens may improve disease outcomes and provide a novel treatment approach for ulcerative colitis.

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螺旋旋毛虫抗原对炎症性肠病的免疫治疗和预防潜力

溃疡性结肠炎（UC）是一种严重的结肠慢性炎症性疾病，是炎症性肠病（IBD）的一种，影响人类和包括猫和狗在内的几种家养动物。螺旋体感染与自身免疫性疾病呈反比关系，正如 "卫生假说 "所解释的那样，"卫生假说 "认为，在螺旋体感染普遍的国家，IBD并不常见，但在发达国家则更为流行。本研究调查了螺旋毛癣菌（T. spiralis）抗原在 IBD 实验性结肠炎模型中的治疗和预防潜力。小鼠分为八组：正常模型组、结肠炎模型组、幼虫抗原预防组、成虫抗原预防组、幼虫抗原治疗组、成虫抗原治疗组、幼虫抗原预防和治疗组、成虫抗原预防和治疗组。通过直肠内注射单剂量 0.2 毫升醋酸诱发结肠炎，健康组除外，该组接受的是 PBS（0.2 毫升）。小鼠在诱导结肠炎 12 天后安乐死。通过结肠炎严重程度以及组织病理学、免疫学和免疫组化检查评估了螺旋体抗原的治疗和预防潜力。结果显示，与结肠炎模型相比，第四至第八组的疾病活动指数（DAI）明显降低，鹅口疮细胞酸性粘蛋白水平升高，iNOS和TNF-α表达减少，血清中IFN-γ和IL-10细胞因子水平降低，尤其是同时接受成虫抗原预防和治疗的组。这项研究表明，螺旋体抗原，尤其是来自成虫的抗原，对实验性结肠炎具有保护和治疗作用，在结肠炎诱导前后给药，通过减轻炎症和调节免疫反应，效果更佳。因此，螺旋体抗原可改善疾病预后，为溃疡性结肠炎提供一种新的治疗方法。

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.