Detection of Giardia and Cryptosporidium in surface water of a subarctic city

IF 2.9 Q2 PARASITOLOGY

Food and Waterborne Parasitology Pub Date : 2025-04-11 DOI:10.1016/j.fawpar.2025.e00262

Christina A. Ahlstrom , Michael P. Carey , Damian M. Menning , Jonathan A. O'Donnell , Andrew M. Ramey

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引用次数: 0

Abstract

Giardia and Cryptosporidium spp. are globally distributed protozoan parasites that can cause gastrointestinal disease in humans and animals. These zoonotic parasites and their ecological relationships have been understudied in Alaska and elsewhere, despite being identified as priority zoonotic pathogens. We aimed to detect and characterize Giardia and Cryptosporidium spp. in waterbodies within Anchorage, Alaska, USA using two methods, including the Environmental Protection Agency (EPA) Method 1623 that relies on microscopy and a molecular detection approach. The molecular approach was ultimately unsuccessful and therefore only data obtained using Method 1623 are presented. Giardia or Cryptosporidium spp. was detected from nine of 15 urban streams and lakes sampled (60 %), six of which were positive for both parasites (40 %). Fewer than 10 cysts or oocysts were detected in 10 L of surface water. Further research to characterize Giardia and Cryptosporidium beyond the genus level would help elucidate the zoonotic potential and ecology of these parasites within the region and more broadly in Alaska.

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某亚北极城市地表水中贾第鞭毛虫和隐孢子虫的检测

贾第虫和隐孢子虫属是分布于全球的原生动物寄生虫，可导致人类和动物的胃肠道疾病。这些人畜共患病寄生虫及其生态关系在阿拉斯加和其他地区一直未得到充分研究，尽管它们已被确定为重点人畜共患病病原体。我们的目标是使用两种方法检测美国阿拉斯加州安克雷奇市水体中的贾第鞭毛虫和隐孢子虫属，包括依赖显微镜的环境保护局（EPA）1623 方法和分子检测方法。分子检测方法最终未获成功，因此本文仅介绍使用方法 1623 获得的数据。在取样的 15 条城市溪流和湖泊中，有 9 条（60%）检测到贾第虫或隐孢子虫属，其中 6 条（40%）对两种寄生虫均呈阳性。在 10 升地表水中检测到的包囊或卵囊少于 10 个。对贾第鞭毛虫和隐孢子虫进行进一步的研究，使其超出种属水平，将有助于阐明这些寄生虫在该地区以及阿拉斯加更广泛地区的人畜共患可能性和生态学。

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.