Human Microbiome Journal最新文献_第7页

Microbial community profiling of peripheral blood in myalgic encephalomyelitis/chronic fatigue syndrome 肌痛性脑脊髓炎/慢性疲劳综合征患者外周血微生物群落分析

Human Microbiome Journal Pub Date : 2018-08-01 DOI: 10.1016/j.humic.2018.05.003

Jeremy E. Ellis, Dara S. Missan, Matthew Shabilla, Delyn Martinez, Stephen E. Fry

{"title":"Microbial community profiling of peripheral blood in myalgic encephalomyelitis/chronic fatigue syndrome","authors":"Jeremy E. Ellis, Dara S. Missan, Matthew Shabilla, Delyn Martinez, Stephen E. Fry","doi":"10.1016/j.humic.2018.05.003","DOIUrl":"10.1016/j.humic.2018.05.003","url":null,"abstract":"<div><p>Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is estimated to afflict hundreds of thousands, if not millions, of Americans with vastly more impacted individuals worldwide; however, the etiology of this disease has not been well established. Based on the features of ME/CFS, we hypothesized an unrecognized vascular infection may be involved. To evaluate this possibility, we performed a blinded pilot study of 30 ME/CFS patients meeting the Fukuda criteria and 48 normal controls. A community-wide analysis using next-generation DNA sequencing methods detected prokaryotic and eukaryotic populations in the peripheral blood of both ME/CFS patients and normal controls. Analysis of the prokaryotic portion of the samples revealed that organisms belonging to the <em>Pseudomonas asplenii</em> species, <em>Pseudomonadaceae</em> family, <em>Pseudomonadales</em> order, and <em>γ-proteobacteria</em> class are inversely correlated with RAND-36 scores, a quality of life metric that is reduced in ME/CFS patients. In addition, analysis of the detected eukaryotic species suggests that the <em>Perkinsus</em> genus is also inversely associated with RAND-36 scores. The most frequently observed eukaryotic DNA was for <em>Funneliformis mosseae</em>, an arbuscular mycorrhizal fungus, which was in both ME/CFS and normal control samples. A multivariate composite score consisting of the <em>Perkinsus</em> genus, <em>Spumella</em> genus, and <em>β-proteobacteria</em> class displays an inverse relationship to RAND-36 scores. Lastly, the combined measurements of several taxa allow for a retrospective categorical sorting of ME/CFS patients from normal control samples. These results suggest that microbial DNA signatures, including those from poorly understood eukaryotes, may be differentially detectable in ME/CFS and normal control samples.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48085435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Colonization of breastfed infants by Bifidobacterium longum subsp. infantis EVC001 reduces virulence gene abundance 长双歧杆菌亚种在母乳喂养婴儿中的定植。婴儿EVC001降低毒力基因丰度

Human Microbiome Journal Pub Date : 2018-08-01 DOI: 10.1016/j.humic.2018.05.001

Giorgio Casaburi , Steven A. Frese

引用次数: 30

Gut microbiota diversity according to dietary habits and geographical provenance 根据饮食习惯和地理来源的肠道微生物群多样性

Human Microbiome Journal Pub Date : 2018-04-01 DOI: 10.1016/j.humic.2018.01.001

Bruno Senghor , Cheikh Sokhna , Raymond Ruimy , Jean-Christophe Lagier

{"title":"Gut microbiota diversity according to dietary habits and geographical provenance","authors":"Bruno Senghor , Cheikh Sokhna , Raymond Ruimy , Jean-Christophe Lagier","doi":"10.1016/j.humic.2018.01.001","DOIUrl":"10.1016/j.humic.2018.01.001","url":null,"abstract":"<div><p>The gut microbiota is an ecosystem including all bacterial species that permanently colonize the gastro intestinal tract and a large number of other microorganisms from the environment. These millions of microorganisms may be unbalanced by a number of external and internal factors. The aim of this review is to summarize recent findings on animal and human studies on the effect of dietary and geographical provenance on gut microbiota-composition. It includes results on the influence of dietary products, type of diet (e.g. vegetarian and omnivorous subgroups), and geographic areas, as well as differences between populations within the same area. In animal models, most results showed contradictory effects on modulation of the intestinal microbiota within the same phylum, while in human studies, dietary products, such as fat, are often reported as being associated with an increase in Bacteroidetes and Actinobacteria species and a decrease in those in the Firmicutes and Proteobacteria phylum. The results of different studies showed that the omnivorous group has a higher diversity of bacteria compared to vegetarians. Gut microbiota composition differs widely between different areas and between different ethnic groups within the same area. However, a higher diversity of bacteria species was encountered in the African population. The conclusions highlight that gut microbiota composition differs according to diet and eating habits which are closely correlated to geographical location suggesting therefore, the need for more in-depth research, looking at ethnic diversity and eating habits.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48088477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 171

New insights in gut microbiota and mucosal immunity of the small intestine 肠道菌群和小肠粘膜免疫的新认识

Human Microbiome Journal Pub Date : 2018-04-01 DOI: 10.1016/j.humic.2018.01.004

Matthieu Million , Julie Tomas , Camille Wagner , Hugues Lelouard , Didier Raoult , Jean-Pierre Gorvel

{"title":"New insights in gut microbiota and mucosal immunity of the small intestine","authors":"Matthieu Million , Julie Tomas , Camille Wagner , Hugues Lelouard , Didier Raoult , Jean-Pierre Gorvel","doi":"10.1016/j.humic.2018.01.004","DOIUrl":"10.1016/j.humic.2018.01.004","url":null,"abstract":"<div><p>Beyond host genetics, the environment determines microbiota-immunity interactions. Most recent studies have focused on the interconnections between micronutrients, microbial and immune populations. However, the control of the gut oxidative stress and redox status has been neglected. Oxidative stress sensitive (Ox-S) prokaryotes include butyrate producers and minority mucosa-associated immunogenic symbionts, such as specific <em>Lactobacillus</em> strains, <em>Bifidobacterium adolescentis</em>, and segmented filamentous bacteria which exemplify the mucosal “minority report” paradigm. Butyrate, produced by <em>Lachnospiraceae</em>, <em>Ruminococcaceae</em> and <em>Bacteroidetes</em>, is the main microbiota-derived gut mucosal immunity regulator and the best functional marker of the healthy mature anaerobic gut microbiota (HMAGM). Oxidative stress during the “window of opportunity” around weaning is observed in severe acute malnutrition and results in Ox-S prokaryote depletion, HMAGM disruption, collapse of butyrate production and durable gut mucosal immunity alteration. High saturated-fat diet leads to oxidative stress, selection of oxidative stress-resistant (Ox-R) <em>Lactobacillus reuteri</em> strains in Peyer’s patches, secretion of pro-inflammatory cytokines, disruption of mucosal immune compartmentalization (leaky gut) and obesity. Beyond dietary micronutrient diversity and pathogen control, future research should focus on antioxidants, control of oxidative stress and Ox-S gut prokaryote preservation as new instrumental targets for maintenance of the gut microbiota-immunity symbiotic loop and prevention of malnutrition and obesity.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43465466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Clostridium botulinum type A-virulome-gut interactions: A systems biology insight A型肉毒杆菌-病毒组-肠道相互作用:系统生物学的见解

Human Microbiome Journal Pub Date : 2018-04-01 DOI: 10.1016/j.humic.2018.01.003

P. Chellapandi, A. Prisilla

{"title":"Clostridium botulinum type A-virulome-gut interactions: A systems biology insight","authors":"P. Chellapandi, A. Prisilla","doi":"10.1016/j.humic.2018.01.003","DOIUrl":"10.1016/j.humic.2018.01.003","url":null,"abstract":"<div><p><em>Clostridium botulinum</em> is a foodborne bacterium capable of producing a potent botulinum neurotoxin with seven serotypes (A–G). Type A strains are being a great concern for causing foodborne, infant and wound botulism in worldwide. Antibacterial resistance is a not a big problem for treating diseases caused by this organism, but antitoxin treatment available today has not been reverse the paralysis. <em>C. botulinum</em> strain Hall A Sanger is a clinically important strain studied intensively for its biochemical and molecular characteristics. Gene cluster for botulinum toxin is strain-specific in nature, which might have evolved independently of each other. Type A strains have a common mechanism for transcription and metabolic regulation of botulinum toxin. BotR is a known transcriptional regulator that controls the expression of botulinum toxin in type A strains in response to nutritional factors in the gut. Two-component system is a key regulator required for the full virulence of this bacterium underlying response to the host and environmental factors. Amino-acid induced germination and chitin catabolic systems are firmly established in this organism, performing the separate processes of toxin or virulence factor synthesis, sporulation and germination. Several virulence factors have recently been identified from this genome, but molecular function of them in the gut of humans is not yet to be known. Genome-scale models are being as an integrated knowledge base for detailed understanding of its host-microbe interactions during the intoxication process.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.01.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49251907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Gut microbiota modifications and weight gain in early life 早期肠道菌群改变与体重增加

Human Microbiome Journal Pub Date : 2018-04-01 DOI: 10.1016/j.humic.2018.01.002

Emmanouil Angelakis, Didier Raoult

{"title":"Gut microbiota modifications and weight gain in early life","authors":"Emmanouil Angelakis, Didier Raoult","doi":"10.1016/j.humic.2018.01.002","DOIUrl":"10.1016/j.humic.2018.01.002","url":null,"abstract":"<div><p>Childhood and adolescent obesity is a significant public health concern and has been associated with cardiovascular disease and related metabolic sequelae later in life. In recent years, several studies have postulated an imbalance in the composition of the early life gut microbiota results in pediatric obesity and its associated diseases. The early life gut microbiota is influenced by several factors including the mode of delivery, prematurity, breastfeeding, and the use of antibiotics and probiotics. It has been proposed that, when given early in life, antibiotics and probiotics disrupt the gut microbiota and consequently its metabolic activity, promoting weight gain. Probiotics have increasingly been administrated to children and studies on the perinatal use of probiotics on low birth weight and healthy infants revealed significantly increased body length and weight later in life in comparison with infants who did not receive probiotic supplements. Similarly, exposure to antibiotics is very high perinatally and in the early periods of life and there is evidence that antibiotic treatment decreases the biodiversity of the early life gut microbiota. In addition, studies have revealed that antibiotic treatment during the first months of life is associated with being overweight later in life. In this paper we review the effects of the administration of probiotics and antibiotics in early life on the gut microbiota and discuss their effects on weight gain.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43927467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

The diabetic foot microbiota: A review 糖尿病足微生物群:综述

Human Microbiome Journal Pub Date : 2017-12-01 DOI: 10.1016/j.humic.2017.09.002

J. Jneid , J.P. Lavigne , B. La Scola , N. Cassir

引用次数: 79

Long-term stability in the gut microbiome over 46 years in the life of Billy Apple® 在比利苹果®的生命中，肠道微生物群的长期稳定性超过46 年

Human Microbiome Journal Pub Date : 2017-12-01 DOI: 10.1016/j.humic.2017.09.001

Thilini N. Jayasinghe , Craig Hilton , Peter Tsai , Billy Apple , Peter Shepherd , Wayne S. Cutfield , Justin M. O'Sullivan

引用次数: 8

Gut microbiota diversity and T1DM onset: Preliminary data of a case-control study 肠道菌群多样性与T1DM发病:一项病例对照研究的初步数据

Human Microbiome Journal Pub Date : 2017-12-01 DOI: 10.1016/j.humic.2017.11.002

Deborah Traversi , Ivana Rabbone , Maria Giovanna Ignaccolo , Giulia Carletto , Irene Racca , Camilla Vallini , Violetta Andriolo , Francesco Cadario , Silvia Savastio , Roberta Siliquini , Franco Cerutti

引用次数: 9

Evidence of giant viruses of amoebae in the human gut 人类肠道中存在巨型变形虫病毒的证据

Human Microbiome Journal Pub Date : 2017-12-01 DOI: 10.1016/j.humic.2017.11.001

Philippe Colson, Sarah Aherfi, Bernard La Scola

{"title":"Evidence of giant viruses of amoebae in the human gut","authors":"Philippe Colson, Sarah Aherfi, Bernard La Scola","doi":"10.1016/j.humic.2017.11.001","DOIUrl":"10.1016/j.humic.2017.11.001","url":null,"abstract":"<div><p>The study of the gut microbiome and virome has developed dramatically since the beginning of the 21st century. Nevertheless, giant viruses of amoebae, which are emerging viruses first described in 2003, have been largely neglected in virome investigations because they are bigger than classical viruses and devoid of ribosomal DNA. Dozens of these viruses have been isolated between 2003 and 2016, which were classified in at least 7 lineages including 2 new recognized virus families. These viruses challenge previous paradigms on viruses and share many characteristics with intra-cellular microbes. We reviewed here findings about the presence of these giant viruses of amoebae in the human gut, whose microbiota has been extensively studied during the last decade. Contrasting with what is currently done for classical viruses, many studies investigating the presence of giant viruses of amoebae have been conducted by culture on amoebae in first intention. To date, a mimivirus and a marseillevirus have been isolated from human feces, which indicates that they can still replicate after a stay in the gut. Besides, sequences related to giant viruses of amoebae have been detected in several metagenomes generated from human feces. Water is a likely source of human exposure to giant viruses of amoebae. The clinical or biological significance of the presence of these viruses in the human gut remains to be determined. Taken together, available findings warrant searching more extensively and systematically for giant viruses of amoebae in the human gut, along with in other body sites.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2017.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44936715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11