Bioprinting最新文献

{"title":"Evolution, integration, and challenges of 3D printing in pharmaceutical applications: A comprehensive review","authors":"Jyoti Kumari ,&nbsp;Shalini Pandey ,&nbsp;Krishna Kant Jangde ,&nbsp;Palanirajan Vijayaraj Kumar ,&nbsp;Dinesh Kumar Mishra","doi":"10.1016/j.bprint.2024.e00367","DOIUrl":"10.1016/j.bprint.2024.e00367","url":null,"abstract":"<div><div>Three-dimensional (3D) printing involves fabricating objects from digital designs by sequentially layering materials along the X, Y, and Z axes. Although this technology has existed since the 1960s, its adoption in the pharmaceutical industry remains limited. This review examines the evolution of 3D printing and its emerging significance in pharmaceuticals. The technique offers numerous advantages, such as product customization, cost-effectiveness, and efficient material usage. Several methods—such as inkjet printing, extrusion printing, and beam-based printing—are employed, utilizing materials ranging from lactose and hydroxypropyl methylcellulose to bioinks like chitosan and hyaluronic acid. Among these techniques, fused deposition modelling (FDM) is particularly noteworthy for its versatility in both biodegradable and non-biodegradable applications. Advances in 3D printing have paved the way for innovative pharmaceutical uses, including the production of complex oral dosage forms, drug delivery systems, and medical devices such as prosthetics. More recent breakthroughs have extended into bioprinting, organ-on-a-chip technologies, and robotics. However, several challenges hinder broader adoption, including limited compatibility with thermosensitive materials, difficulties in scaling production, and maintaining quality control. Additionally, the lack of standardized regulatory and ethical frameworks for clinical approval complicates progress. This review explores the key 3D printing techniques, materials, and trends relevant to pharmaceuticals, while addressing resource constraints, intellectual property issues, and regulatory hurdles. It concludes by identifying future directions for research and development, emphasizing the need to optimize these technologies for widespread pharmaceutical applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00367"},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering bone regeneration: A review of cutting-edge scaffolds in tissue engineering","authors":"Y. Alex ,&nbsp;Sumi Vincent ,&nbsp;Nidhin Divakaran ,&nbsp;U.T. Uthappa ,&nbsp;Parthasarathy Srinivasan ,&nbsp;Suhail Mubarak ,&nbsp;Mamdouh Ahmed Al-Harthi ,&nbsp;Duraisami Dhamodharan","doi":"10.1016/j.bprint.2024.e00364","DOIUrl":"10.1016/j.bprint.2024.e00364","url":null,"abstract":"<div><div>Bone tissue engineering (BTE) is aims to develop advanced strategies to regenerate damaged or diseased bone, through the integration of principles from cellular biology, biomaterials science, and engineering. The vital aspect of these studies includes the design and fabrication of scaffolds that support cell adhesion, proliferation, and differentiation, ultimately promoting the formation of new bone tissue. Recent developments in scaffold materials have focused on organic, inorganic, and composite biomaterials. Each of these showcasing unique and distinct advantages in terms of biocompatibility, biodegradability, and mechanical strength. Polymers, such as poly (lactic-co-glycolic acid) (PLGA), provide flexibility and degradation profiles, which are conducive to tissue integration. While ceramics, including hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), offer mechanical properties similar to native bone. The fusion of organic and inorganic components in composites has yielded scaffolds with enhanced functionality, such as improved osteo-conductivity and controlled degradation rates. Advanced fabrication techniques, particularly electrospinning and 3D printing, have revolutionized scaffold design by enabling precise control over pore size, porosity, and surface architecture, critical parameters for mimicking the extracellular matrix (ECM) of bone. These structural characteristics directly influence cellular behaviors such as migration, proliferation, and differentiation, which are crucial for successful bone regeneration. This review critically evaluates the recent advances in biomaterials for scaffold fabrication, with a focus on optimizing the interplay between material properties and scaffold architecture to improve therapeutic outcomes in bone regeneration. The findings underscore the importance of material selection and scaffold design in BTE and provide actionable insights for both researchers and clinicians in the development of next-generation scaffolds. By synthesizing recent progress in this field, the review highlights potential avenues for future research aimed at refining scaffold materials and fabrication techniques to enhance bone regeneration.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00364"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinted GelMA scaffolds for clinical applications: Promise and challenges","authors":"Soumitra Das,&nbsp;Remya Valoor,&nbsp;Jeyapriya Thimukonda Jegadeesan,&nbsp;Bikramjit Basu","doi":"10.1016/j.bprint.2024.e00365","DOIUrl":"10.1016/j.bprint.2024.e00365","url":null,"abstract":"<div><div>The promise of bioprinting in diverse applications ranging from <em>in vitro</em> drug screening to creating patient-specific tissues/organs for personalized medicine has attracted significant attention globally. In this context, this review discusses the progress being made over the last decades with gelatin methacryloyl (GelMA) as a foundational hydrogel for diverse bioprintable ink formulations in particular relevance to printability, buildability, and bio-functionality. Furthermore, a comprehensive analysis is presented on the recent developments in 3D (bio)printing of GelMA for the reconstruction or regeneration of artificial tissues, spanning musculoskeletal, neurological, cardiovascular, urological, ophthalmological, dermatological, and drug screening of cancer-related applications. While presenting such wide-ranging potential, an emphasis is placed on addressing the key challenges associated with scaling up from small-scale laboratory practices to clinical applications. Furthermore, the review sheds light on the regulatory framework-related issues that impede the widespread clinical usage of 3D bioprinted tissues and organs in patient care. Taken together, this review provides significant insights into the current state of the field for researchers, clinicians, and policymakers, while navigating the intricate landscape of 3D (bio)printing for clinical translation.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00365"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural composite hydrogel laden with mesenchymal stromal cells for osteochondral repair: Comparison between casting and 3D bioprinting","authors":"Marjorie Dufaud ,&nbsp;Christophe Marquette ,&nbsp;Christian Jorgensen ,&nbsp;Emeline Perrier-Groult ,&nbsp;Danièle Noël","doi":"10.1016/j.bprint.2024.e00366","DOIUrl":"10.1016/j.bprint.2024.e00366","url":null,"abstract":"<div><div>Synovial joints, and particularly the osteochondral unit, are prone to lesions, with high risk of degeneration towards osteoarthritis. Various treatment strategies have been developed, including surgical techniques and cellular therapies, but they all show limitations. In this context, tissue engineering approaches, particularly 3D bioprinting, are promising for generating osteochondral tissue substitutes for joint repair. In this work, two biofabrication techniques, casting and extrusion-based 3D bioprinting, of an optimized formulation of a gelatin/alginate/fibrinogen bioink loaded with murine mesenchymal stromal cells (MSCs) were compared for the generation of cartilage and bone substitutes. Cell viability, proliferation and differentiation were characterized. Both techniques showed similar results in terms of viability and proliferation, but only the 3D bioprinted constructs allowed for differentiation towards the chondrogenic or osteogenic lineage using specific culture media. Bioprinting of biphasic osteochondral constructs comprising a cartilage compartment on top of a bone compartment was also explored. The study highlights the potential of our natural composite hydrogel bioink and extrusion-based 3D bioprinting for the generation of osteochondral tissue substitutes. Although further optimizations are needed, the study laid the groundwork for future advancements in osteochondral tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00366"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D and 4D printed materials for cardiac transplantation: Advances in biogenerative engineering","authors":"Aayush Prakash ,&nbsp;Sathvik Belagodu Sridhar ,&nbsp;Adil Farooq Wali ,&nbsp;Sirajunisa Talath ,&nbsp;Javedh Shareef ,&nbsp;Rishabha Malviya","doi":"10.1016/j.bprint.2024.e00362","DOIUrl":"10.1016/j.bprint.2024.e00362","url":null,"abstract":"<div><div>The most common reason for death worldwide is cardiovascular problems, and current treatments including medication, surgery, and heart transplants have disadvantages. Both 3D and 4D printing technologies are being investigated due to the demand for sophisticated solutions in cardiac care. With the use of these technologies, it may be possible to construct intricate circulatory systems, provide individualized care, and find solutions to problems like organ shortages and immune rejection. The paper focuses on various bioprinting methods that may be used in cardiac tissue engineering to create biomimetic structures, improve vascularization, and construct functional heart tissues using 3D and 4D manufacturing. The advancement of 3D and 4D printing procedures has led to substantial advancements in heart tissue engineering by offering precise and customized solutions. These technologies make it possible to fabricate intricate cardiovascular models along with medical equipment, which improves surgical planning and allows for patient-specific therapies. There are still challenges to be solved, primarily in the areas of realistic vascularization and the use of biomaterials that resemble natural cardiac tissue in terms of their mechanical and chemical properties. Technologies for 3D and 4D printing hold promise for resolving major issues with heart transplantation, namely donor scarcity and rejection. Improving vascularization along with biomaterial incorporation for therapeutic applications has to be the main goal of future research.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00362"},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of toxicity testing platforms from 2D to advanced 3D bioprinting for safety assessment of drugs","authors":"Rohin Shyam ,&nbsp;Rinni Singh ,&nbsp;Mukul Bajpai ,&nbsp;Arunkumar Palaniappan ,&nbsp;Ramakrishnan Parthasarathi","doi":"10.1016/j.bprint.2024.e00363","DOIUrl":"10.1016/j.bprint.2024.e00363","url":null,"abstract":"<div><div>The process involved in the discovery of novel drugs in medical sciences is challenging due to the time-intensive process that results in a high cost of development. Additionally, it is reported that 90 % of new drugs fail in clinical trials and cannot reach the market. One of the primary reasons for failure is that research laboratories and pharmaceutical companies have been relying exclusively on data derived from animal-based models for testing the efficacy and safety of newly developed drugs. These models do not completely recapitulate human physiology or pathophysiology, resulting in a lower translational rate. Further, the evaluation of toxicity of drugs to the human body requires a more robust and holistic approach. Researchers across the globe are focusing on developing <em>in vitro</em>3D models as alternatives to traditional animal testing to circumvent these challenges. These model systems could replicate and mimic the human physiological microenvironment, cellular interactions, and arrangements. <em>In vitro</em>3D models would provide improved methods to evaluate and comprehend drug response, thereby reducing the burden on animal usage. Further, reducing the time and costs associated with developing, screening, drug failure, and translation of drugs is also realizable. In this communication, existing <em>in vitro</em> 3D models that are used in the drug development process are reviewed. In addition, the advancements in using 3D bioprinting and organ-on-a-chip technologies towards generating human reconstructed tissues/organs are also highlighted. The challenges from a technological and regulatory perspective on adapting these alternate animal models are also discussed.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00363"},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robust design optimization of Critical Quality Indicators (CQIs) of medical-graded polycaprolactone (PCL) in bioplotting","authors":"Nectarios Vidakis ,&nbsp;Markos Petousis ,&nbsp;Constantine David ,&nbsp;Dimitrios Sagris ,&nbsp;Nikolaos Mountakis ,&nbsp;Mariza Spiridaki ,&nbsp;Amalia Moutsopoulou ,&nbsp;Nektarios K. Nasikas","doi":"10.1016/j.bprint.2024.e00361","DOIUrl":"10.1016/j.bprint.2024.e00361","url":null,"abstract":"<div><div>Polycaprolactone (PCL), either in its pure grade or as a polymeric matrix for bio-composites, plays a key role in the biomedical and bioengineering industries. It is also considered a multifunctional and versatile polymer for bioprinting and bioplotting purposes, especially in tissue engineering. Herein, an undiscovered yet valuable aspect of PCL extrusion-based bioprinting, such as the predictability of Critical Quality Indicators (CQIs), is investigated in depth. With the aid of the robust L25 orthogonal matrix design, the six most generic and device-independent control factors proved their impact on quality metrics such as global porosity, dimensional conformity, and surface roughness, determined with the aid of highly evolved Nondestructive Testing (NDT) and algorithms. To this end, 25 experimental runs were set, and 125 specimens were fabricated using an industrial-scale bio-plotter and medical-graded polycaprolactone. Various infill densities (ID), layer thicknesses (LT), raster deposition angles (RDA), printing speeds (PS), nozzle temperatures (NT), and bed temperatures (BT) were applied. CQIs were determined using optical profilometry and microscopy, and micro-computed tomography. Quadratic predictive equations were compiled and verified using two additional, well-chosen experimental runs. These generally applicable predictive models carry a massive amount of research and industrial merit, as they ensure visibility in bioprinting with PCL.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00361"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the development of stereolithography-based additive manufacturing processes: A review of applications and challenges","authors":"Ambreen Afridi,&nbsp;Ans Al Rashid,&nbsp;Muammer Koç","doi":"10.1016/j.bprint.2024.e00360","DOIUrl":"10.1016/j.bprint.2024.e00360","url":null,"abstract":"<div><div>Additive manufacturing processes have progressed over recent years due to their superiority over conventional manufacturing methods. Their ability to fabricate materials with complex structures, increased precision, and reduced cost have opened avenues for various industrial applications, including biomedical, electrical, mechanical, aviation, and filtration, and led to their development over time. Stereolithography (SLA) is an additive manufacturing technique, through photopolymerization reaction, it solidifies a selective resin to produce three-dimensional objects. SLA has emerged as a leading 3D printing technique, revolutionizing prototyping and production across various industries. SLA has been through four generations of development and advancement, resulting in its improved performance, the diversity of materials, and the variety of applications. Stereolithography has diversified its material and emerged as a promising method for polymer-based composite when operating under optimized conditions. SLA offers superior resolution, high finish quality, improved speed and precision, and is cost-effective compared to alternative techniques like Fused Deposition Modeling (FDM). This current study aims to comprehensively review SLA development, its processes, applications and inherent challenges in mechanical, electrical and biomedical fields.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00360"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing biomaterial inks: A study on the printability of Carboxymethyl cellulose-Laponite nanocomposite hydrogels and dental pulp stem cells bioprinting","authors":"Ingri Julieth Mancilla Corzo ,&nbsp;Jessica Heline Lopes da Fonseca ,&nbsp;Victor Ferman ,&nbsp;Diego Noé Rodríguez Sánchez ,&nbsp;Alexandre Leite Rodrigues de Oliveira ,&nbsp;Marcos Akira d'Ávila","doi":"10.1016/j.bprint.2024.e00358","DOIUrl":"10.1016/j.bprint.2024.e00358","url":null,"abstract":"<div><p>Tissue engineering approaches require biocompatible materials with precise pre-designed geometry, shape fidelity, and promote cellular functions. Addressing these requirements, our study focused on developing an optimized bioink formulation using carboxymethyl cellulose (CMC) and Laponite hydrogels tailored for extrusion-based three-dimensional bioprinting. To this, we investigated the rheological properties and filament behavior before and during printing. As Laponite concentration increased in CMC solutions, it improved shear-thinning behavior, viscosity, and storage modulus, resulting in well-defined filament characteristics with lower diffusion rates, excellent shape fidelity, and robust printability. Thus, we achieved a suitable biomaterial ink formulation with concentrations of 1 wt% of CMC and 4 wt% of Laponite (1C4L). Subsequently, a statistical analysis guided us to select the optimal parameters for large-scale construct printing: a nozzle speed of 5 mm/s, a print distance of 0.41 mm, and an extrusion multiplier of 1.35. After that, we enhanced the structural integrity of printed hydrogels through ionic crosslinking with calcium chloride (CaCl₂) and citric acid (CA), revealing higher-strength hydrogels at higher concentrations of CaCl₂. Finally, we have confirmed the groundbreaking potential of our bioink by integrating dental pulp mesenchymal stem cells (DPSC) into the 1C4L ink. Our bioprinted constructs showed optimized swelling, non-toxic effects, and retained excellent shape fidelity, crucial for creating anatomically accurate tissues. Our findings provide crucial insights linking the rheological analysis, the bioprinting process, and the biological properties of hydrogels, paving the way for their use for tissue engineering and other biomedical applications.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00358"},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision 3D printing of chitosan-bioactive glass inks: Rheological optimization for enhanced shape fidelity in tissue engineering scaffolds","authors":"Larissa R. Lourenço ,&nbsp;Roger Borges ,&nbsp;Danilo Carastan ,&nbsp;Mônica B. Mathor ,&nbsp;Juliana Marchi","doi":"10.1016/j.bprint.2024.e00359","DOIUrl":"10.1016/j.bprint.2024.e00359","url":null,"abstract":"<div><div>3D printing technology in tissue engineering applications provides several advantages for scaffold development, especially with natural materials, such as chitosan, which provides a biomimetic environment for cellular growth. However, chitosan hydrogel-based inks still show poor printing fidelity. In this article, we overcame this challenge by incorporating bioactive glasses (BG) nanoparticles (up to 5 wt%) into the chitosan hydrogel. The resulting inks were characterized by rheological tests, while their processability was evaluated through measurements of shape fidelity. An indirect cytotoxicity assay was also conducted to evaluate the cell viability of the printed scaffolds. The results indicated that adding BG nanoparticles to the chitosan-based ink modified its rheological properties and improved its shape-fidelity during 3D printing, which we suggest are consequences of hydrogen bonds established between the glass and the chitosan chains. Also, cytotoxicity assessment demonstrated that the resulting scaffold exhibits high cell viability. In conclusion, the proposed composite ink has optimized rheological properties for 3D printing and is promising for applications in tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00359"},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}