Non-coding RNA Research最新文献

{"title":"Emerging roles of non-coding RNAs in modulating the PI3K/Akt pathway in cancer","authors":"Mehrdad Hashemi ,&nbsp;Elaheh Mohandesi Khosroshahi ,&nbsp;Saba Asadi ,&nbsp;Mahsa Tanha ,&nbsp;Forough Ghatei Mohseni ,&nbsp;Ramina Abdolmohammad Sagha ,&nbsp;Elham Taheri ,&nbsp;Paria Vazayefi ,&nbsp;Helya Shekarriz ,&nbsp;Fatemeh Habibi ,&nbsp;Shaghayegh Mortazi ,&nbsp;Ramin Khorrami ,&nbsp;Noushin Nabavi ,&nbsp;Mohsen Rashidi ,&nbsp;Afshin Taheriazam ,&nbsp;Payman Rahimzadeh ,&nbsp;Maliheh Entezari","doi":"10.1016/j.ncrna.2024.08.002","DOIUrl":"10.1016/j.ncrna.2024.08.002","url":null,"abstract":"<div><p>Cancer progression results from the dysregulation of molecular pathways, each with unique features that can either promote or inhibit tumor growth. The complexity of carcinogenesis makes it challenging for researchers to target all pathways in cancer therapy, emphasizing the importance of focusing on specific pathways for targeted treatment. One such pathway is the PI3K/Akt pathway, which is often overexpressed in cancer. As tumor cells progress, the expression of PI3K/Akt increases, further driving cancer advancement. This study aims to explore how ncRNAs regulate the expression of PI3K/Akt. NcRNAs are found in both the cytoplasm and nucleus, and their functions vary depending on their location. They can bind to the promoters of PI3K or Akt, either reducing or increasing their expression, thus influencing tumorigenesis. The ncRNA/PI3K/Akt axis plays a crucial role in determining cell proliferation, metastasis, epithelial-mesenchymal transition (EMT), and even chemoresistance and radioresistance in human cancers. Anti-tumor compounds can target ncRNAs to modulate the PI3K/Akt axis. Moreover, ncRNAs can regulate the PI3K/Akt pathway both directly and indirectly.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"10 ","pages":"Pages 1-15"},"PeriodicalIF":5.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001276/pdfft?md5=9218fb05bcaa11abdb9f4fa80b5713ea&pid=1-s2.0-S2468054024001276-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142041329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting LINC00665/miR-199b-5p/SERPINE1 axis to inhibit trastuzumab resistance and tumorigenesis of gastric cancer via PI3K/AKt pathway","authors":"Bingyu Wang,&nbsp;Wenbo Liu,&nbsp;Buyun Song,&nbsp;Yong Li,&nbsp;Yingying Wang,&nbsp;Bibo Tan","doi":"10.1016/j.ncrna.2024.07.004","DOIUrl":"10.1016/j.ncrna.2024.07.004","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) serve as critical mediators of tumor progression and drug resistance in cancer. Herein, we identified a lncRNA, LINC00665, associated with trastuzumab resistance and development in gastric cancer (GC). LINC00665 was highly expressed in GC tissues and high expression of LINC00665 was correlated with poor prognosis. LINC00665 knockdown was verified to suppress migration, invasion, and resistance to trastuzumab in GC. Furthermore, we found that LINC00665 participates in the infiltration of naive B cells, mast cells, and T follicular helper (Tfh) cells. Mechanistically, LINC00665 was confirmed to regulate tumorigenesis and trastuzumab resistance by activating PI3K/AKt pathway. LINC00665 sponged miR-199b-5p to interact with SERPINE1 expression, resulting in the increase of phosphorylation of AKt, thus participating in the PI3K/AKt pathway. To summarize, LINC00665 facilitated the tumorigenesis and trastuzumab resistance of GC by sponging miR-199b-5p and promoting SERPINE1 expression, which further activated PI3K/AKt signaling; this finding reveals a new mechanism by which LINC00665 modulates tumor development and drug resistance in GC.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"10 ","pages":"Pages 153-162"},"PeriodicalIF":5.9,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanosensitive lncRNA H19 promotes chondrocyte autophagy, but not pyroptosis, by targeting miR-148a in post-traumatic osteoarthritis","authors":"Xuchang Zhou ,&nbsp;Hong Cao ,&nbsp;Tao Liao ,&nbsp;Weizhong Hua ,&nbsp;Ruobing Zhao ,&nbsp;Dongxue Wang ,&nbsp;Huili Deng ,&nbsp;Yajing Yang ,&nbsp;ShengYao Liu ,&nbsp;Guoxin Ni","doi":"10.1016/j.ncrna.2024.07.005","DOIUrl":"10.1016/j.ncrna.2024.07.005","url":null,"abstract":"<div><h3>Objective</h3><div>Investigating whether mechanosensitive lncRNA H19 can directly target miR-148a to alleviate cartilage damage in post-traumatic osteoarthritis (PTOA).</div></div><div><h3>Methods</h3><div>Thirty-two female rats were randomly divided into four groups: Sham-operated group (Sham group, n = 8), treadmill running group (R group, n = 8), anterior cruciate ligament transection (ACLT) group (ACLT group, n = 8), and ACLT + treadmill running group (ACLT + R group, n = 8). Histological evaluation was performed to observe the pathological changes in the cartilage of the rat knee. Micro-CT was performed to detect the bone morphological changes in the subchondral bone. RT-qPCR and Western-Blot were performed to detect changes in mRNA and protein levels of metabolic and inflammatory factors as well as changes in the expression of lncRNA H19 and miR-148a in cartilage. The Flexcell 5000™ Tension System was used to further validate that lncRNA H19 has mechanosensitivity <em>in vitro</em>. Finally, cell transfection techniques were used to knock down the expression of lncRNA H19 in chondrocytes to validate the regulatory role of lncRNA H19/miR-148a in cartilage metabolism.</div></div><div><h3>Results</h3><div>ACLT combined with treadmill running aggravated the abnormal hyperplasia of subchondral bone in the lateral tibial plateau of the rat knee joint, disturbed the balance of cartilage metabolism, induced cartilage inflammatory response and chondrocyte pyroptosis, which eventually led to cartilage damage and PTOA. Importantly, we found that the expression of lncRNA H19 was significantly downregulated in the cartilage of the ACLT + R group. Bioinformatics analysis revealed that miR-148a may be a direct target of lncRNA H19. Subsequently, we focused on the mechanosensitive of lncRNA H19. Subsequently, moderate-intensity mechanical tension stress reversed the expression of lncRNA H19 and autophagy-related factors in inflammatory chondrocytes, while miR-148a showed an opposite expression trend, demonstrating that mechanosensitive lncRNA H19 may be involved in regulating the chondrocyte inflammatory response by targeting miR-148a and activating autophagy. Cell transfection experiments revealed that lncRNA H19 knockdown upregulated miR-148a expression and significantly inhibited the autophagy level of chondrocytes without significant alteration of chondrocyte pyroptosis, which in turn exacerbated the inflammatory response of chondrocytes.</div></div><div><h3>Conclusions</h3><div>Mechanosensitive lncRNA H19 can promote chondrocyte autophagy rather than pyroptosis by targeting miR-148a, thus alleviating cartilage damage in PTOA. LncRNA H19 may be a potential therapeutic target for PTOA.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"10 ","pages":"Pages 163-176"},"PeriodicalIF":5.9,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current landscape of exosomal non-coding RNAs in prostate cancer: Modulators and biomarkers","authors":"Yongxing Li ,&nbsp;Xiaoqi Tang ,&nbsp;Binpan Wang ,&nbsp;Ming Chen ,&nbsp;Ji Zheng ,&nbsp;Kai Chang","doi":"10.1016/j.ncrna.2024.07.003","DOIUrl":"10.1016/j.ncrna.2024.07.003","url":null,"abstract":"<div><p>Prostate cancer (PCa) has the highest frequency of diagnosis among solid tumors and ranks second as the primary cause of cancer-related deaths. Non-coding RNAs (ncRNAs), such as microRNAs, long non-coding RNAs and circular RNAs, frequently exhibit dysregulation and substantially impact the biological behavior of PCa. Compared with circulating ncRNAs, ncRNAs loaded into exosomes are more stable because of protection by the lipid bilayer. Furthermore, exosomal ncRNAs facilitate the intercellular transfer of molecules and information. Increasing evidence suggests that exosomal ncRNAs hold promising potential in the progression, diagnosis and prognosis of PCa. This review aims to discuss the functions of exosomal ncRNAs in PCa, evaluate their possible applications as clinical biomarkers and therapeutic targets, and provide a comprehensive overview of the ncRNAs regulatory network in PCa. We also identified ncRNAs that can be utilized as biomarkers for diagnosis, staging, grading and prognosis assessment in PCa. This review offers researchers a fresh perspective on the functions of exosomal ncRNAs in PCa and provides additional options for its diagnosis, progression monitoring, and prognostic prediction.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1351-1362"},"PeriodicalIF":5.9,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001239/pdfft?md5=cae93c74fa74129a7bcbfe8318e0a0aa&pid=1-s2.0-S2468054024001239-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA genetic variations associated with the predisposition of oral squamous cell carcinoma in central Indian population","authors":"Shikha Tiwari ,&nbsp;Ritu Pandey ,&nbsp;Vinay Kumar ,&nbsp;Saikat Das ,&nbsp;Vikas Gupta ,&nbsp;Rajeev Nema ,&nbsp;Ashok Kumar","doi":"10.1016/j.ncrna.2024.07.002","DOIUrl":"10.1016/j.ncrna.2024.07.002","url":null,"abstract":"<div><p>The disease burden of Oral Squamous Cell Carcinoma (OSCC) is rising day-by-day and is expected to rise 62 % through 2035. The chewing of tobacco, areca nut, and betel leaf, poor oral hygiene, and chronic infection are common risk factors of OSCC, but genetic and epigenetic factors also contribute equally. MicroRNAs (miRNAs) are comprised of small, non-coding endogenous RNA that regulate a plethora of biological activities by targeting messenger RNA through degradation or inhibition. Single Nucleotide Polymorphisms (SNPs) in miRNA genes can regulate the development and progression of OSCC. The present study aimed to determine the association between SNPs in miRNA genes (miRSNPs) with the risk of OSCC. A case-control study involving 225 histo-pathologically confirmed OSCC cases and 225 healthy controls was conducted, where 25 miRSNPs were analyzed by iPLEX MassArray analysis. A SNP rs12220909 in <em>MIR4293</em> showed a highly protective effect (<em>CC vs GG,</em> OR = 0.0431, 95%CI = 0.005–0.323, p = 3e-6). Whereas three SNPs<strong>,</strong> namely, rs4705342 in <em>MIR143</em> (<em>CC vs TT</em>, OR = 2.25, 95%CI = 2.00–2.53, p = 0.0008), rs531564 in <em>MIR124</em> (<em>CC vs GG</em>, OR = 24.18, 95%CI = 3.22–181.37, p = 3e-6), and rs3746444 in MIR499 (<em>AA vs GG</em>, OR = 2.01, 95%CI = 1.32–3.05, p = 0.001) were significantly associated with a higher risk of OSCC. Additionally, NanoString-based nCounter miRNA expression profiling revealed that miR-499a (Log2FC = −1.07), and miR-143 (Log2FC = −1.56) were aberrantly expressed in OSCC tissue. Taken together, the above miSNPs may contribute to the high incidence of OSCC in central India. However, further studies with large cohorts and ethnic stratification are required to validate our findings.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1333-1341"},"PeriodicalIF":5.9,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001227/pdfft?md5=76d1043d1af586d58d444dcd510091de&pid=1-s2.0-S2468054024001227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of circulating plasma miR-9, miR-29a, miR-192, and miR-375 as potential biomarkers for predicting prediabetes and type 2 diabetes in Nepali adult population","authors":"Daya Ram Pokharel ,&nbsp;Abhishek Maskey ,&nbsp;Ramchandra Kafle ,&nbsp;Ashim Batajoo ,&nbsp;Prajwal Dahal ,&nbsp;Roji Raut ,&nbsp;Shailesh Adhikari ,&nbsp;Binod Manandhar ,&nbsp;Krishna Das Manandhar","doi":"10.1016/j.ncrna.2024.07.001","DOIUrl":"https://doi.org/10.1016/j.ncrna.2024.07.001","url":null,"abstract":"<div><p>Circulating plasma miRNAs have emerged as potential early predictors of glucometabolic disorders. However, their biomarker potential remains unvalidated in populations with diverse genetic backgrounds, races, and ethnicities. This study aims to validate the biomarker potential of plasma miR-9, miR-29a, miR-192, and miR-375 for early detection of prediabetes and type 2 diabetes mellitus (T2DM) in Nepali populations that represent distinct genetic backgrounds, races, and ethnicities. A total of 46 adults, categorized into healthy controls (n = 25), prediabetes (n = 9), and T2DM (n = 12) groups, were enrolled. Baseline sociodemographic, anthropometric, and clinical characteristics were collected. Fold change in plasma expression of all four miRNAs was quantified using RT-qPCR against the <em>RNU6B</em> reference gene. Their biomarker potential was determined by receiver operating characteristic (ROC) curve analysis. Multivariate discriminant function and hierarchical cluster analyses were used to evaluate the effectiveness of the miRNA panel in reclassifying study participants who were initially categorized according to their glucose tolerance status. Plasma expression of all four miRNAs was significantly upregulated in T2DM patients compared to normoglycemic controls. Furthermore, the expression of only miR-29a and miR-375 was upregulated in T2DM patients than in prediabetic individuals. Notably, only miR-192 expression was significantly upregulated in prediabetic individuals than in the normoglycemic controls. The miRNA expression profiles had the potential of reclassifying the participants into three original groups with an accuracy of 69.6 %. ROC curve analysis identified miR-192 as the predictor for both prediabetes and T2DM, while miR-9, miR-29a, miR-192, and miR-375 were predictive only for T2DM. The specific set of miRNA combinations significantly improved their predictive accuracy. This study validates the early predictive biomarker potential of plasma miR-9, miR-29a, miR-192, and miR-375 also in the Nepali population and paves the way for future translational studies to validate their utility in clinical laboratories.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1324-1332"},"PeriodicalIF":5.9,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001215/pdfft?md5=cf7d48aa296373f1ad87911d4a7d0991&pid=1-s2.0-S2468054024001215-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141607923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of noncoding RNAs in cancer diagnosis, prognosis, and precision medicine","authors":"Basmah Eldakhakhny ,&nbsp;Abdulaziz M. Sutaih ,&nbsp;Moaaz A. Siddiqui ,&nbsp;Yamin M. Aqeeli ,&nbsp;Akram Z. Awan ,&nbsp;Mohammad Y. Alsayegh ,&nbsp;Salma A. Elsamanoudy ,&nbsp;Ayman Elsamanoudy","doi":"10.1016/j.ncrna.2024.06.015","DOIUrl":"https://doi.org/10.1016/j.ncrna.2024.06.015","url":null,"abstract":"<div><p>This review article studies the complex field of noncoding RNAs (ncRNAs) in cancer biology, focusing on their potential use as biomarkers and therapeutic targets. NcRNAs include circular RNAs (circRNAs), long noncoding RNAs (lncRNAs), and microRNAs (miRNAs). We discuss how ncRNAs affect gene expression in cancerous cells, the spread of cancer, and metastasis. The article illustrates the complex pathways through which ncRNAs can affect oncogenesis, tumor suppression, and therapeutic resistance. It also assesses the potential clinical uses of non-coding RNAs (ncRNAs), including their utility in cancer diagnosis, and prognosis. Besides, their potential for personalized cancer therapy is documented. Finally, it is concluded that understanding the role of ncRNA is of clinical importance regarding carcinogenesis, cancer diagnosis, and personalized therapy.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1315-1323"},"PeriodicalIF":5.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001203/pdfft?md5=47b4312e1dda870b46b62172a2c8e84d&pid=1-s2.0-S2468054024001203-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141543166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional roles of conserved lncRNAs and circRNAs in eukaryotes","authors":"Jingxin Li,&nbsp;Xiaolin Wang","doi":"10.1016/j.ncrna.2024.06.014","DOIUrl":"https://doi.org/10.1016/j.ncrna.2024.06.014","url":null,"abstract":"<div><p>Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) have emerged as critical regulators in essentially all biological processes across eukaryotes. They exert their functions through chromatin remodeling, transcriptional regulation, interacting with RNA-binding proteins (RBPs), serving as microRNA sponges, etc. Although non-coding RNAs are typically more species-specific than coding RNAs, a number of well-characterized lncRNA (such as <em>XIST</em> and <em>NEAT1</em>) and circRNA (such as <em>CDR1as</em> and <em>ciRS-7</em>) are evolutionarily conserved. The studies on conserved lncRNA and circRNAs across multiple species could facilitate a comprehensive understanding of their roles and mechanisms, thereby overcoming the limitations of single-species studies. In this review, we provide an overview of conserved lncRNAs and circRNAs, and summarize their conserved roles and mechanisms.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1271-1279"},"PeriodicalIF":5.9,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001197/pdfft?md5=19c5497bfc59039ea171fe4d2a3a8617&pid=1-s2.0-S2468054024001197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiRNA-3163 limits ovarian cancer stem-like cells via targeting SOX-2 transcription factor","authors":"Bilash Chatterjee ,&nbsp;Subhankar Bose ,&nbsp;Richa Singh ,&nbsp;Amit Kumar Dixit ,&nbsp;Lalrin Puia ,&nbsp;Amit Kumar Srivastava","doi":"10.1016/j.ncrna.2024.06.012","DOIUrl":"https://doi.org/10.1016/j.ncrna.2024.06.012","url":null,"abstract":"<div><p>Cancer stem cells (CSCs) are pivotal in both cancer progression and the acquisition of drug resistance. MicroRNAs (miRNAs) play a crucial role in modulating CSC properties and are being explored as potential targets for therapeutic interventions. MiR-3163 is primarily known for its tumor suppressive properties in various human malignancies, with lower expression reported across different cancer types. However, its role in regulating the ovarian CSC phenotype and the underlying mechanism remain largely unknown. Here, we report a remarkable downregulation of miR-3163 in ovarian cancer stem-like cells (CSLCs). Enforced expression of miR-3163 in ovarian adherent and CSLCs, significantly disrupts the stemness phenotype. Moreover, downregulation of miR-3163 expression in ovarian cancer cells (OV2008 and OVCAR-3) inhibits the stem-like cells characterized by CD44+CD117+ expression. Sphere formation assay results reveal that overexpression of miR-3163 in ovarian cancer cells significantly inhibits spheroid formation ability, confirming the regulatory properties of miR-3163 on ovarian CSLCs. Mechanistic investigation reveals that miR-3163 depletes ovarian CSLCs via targeting SOX-2. Furthermore, we establish SOX-2 as a direct target of miR-3163 through dual-luciferase assay. Taken together, our study demonstrates that overexpression of miR-3163 could be a promising strategy for efficiently eradicating the CSC population to prevent chemoresistance and tumor relapse in ovarian cancer patients.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1308-1314"},"PeriodicalIF":5.9,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001173/pdfft?md5=f2b0546ab628ee3dc2fbcab3fce7d88e&pid=1-s2.0-S2468054024001173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141543131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human lncRNAs harbor conserved modules embedded in different sequence contexts","authors":"Francesco Ballesio ,&nbsp;Gerardo Pepe ,&nbsp;Gabriele Ausiello ,&nbsp;Andrea Novelletto ,&nbsp;Manuela Helmer-Citterich ,&nbsp;Pier Federico Gherardini","doi":"10.1016/j.ncrna.2024.06.013","DOIUrl":"https://doi.org/10.1016/j.ncrna.2024.06.013","url":null,"abstract":"<div><p>We analyzed the structure of human long non-coding RNA (lncRNAs) genes to investigate whether the non-coding transcriptome is organized in modular domains, as is the case for protein-coding genes. To this aim, we compared all known human lncRNA exons and identified 340 pairs of exons with high sequence and/or secondary structure similarity but embedded in a dissimilar sequence context. We grouped these pairs in 106 clusters based on their reciprocal similarities. These shared modules are highly conserved between humans and the four great ape species, display evidence of purifying selection and likely arose as a result of recent segmental duplications. Our analysis contributes to the understanding of the mechanisms driving the evolution of the non-coding genome and suggests additional strategies towards deciphering the functional complexity of this class of molecules.</p></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"9 4","pages":"Pages 1257-1270"},"PeriodicalIF":5.9,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468054024001185/pdfft?md5=62872df6d84c679b4b5496dffacbf5d5&pid=1-s2.0-S2468054024001185-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}