Non-coding RNA Research最新文献

Exosome-derived hsa_circ_0007132 promotes lenvatinib resistance by inhibiting the ubiquitin-mediated degradation of NONO 外泌体衍生的hsa_circ_0007132通过抑制泛素介导的NONO降解来促进lenvatinib耐药性

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-15 DOI: 10.1016/j.ncrna.2025.05.007

Mingbo Cao , Yuxuan Li , Xiaorui Su , Yongchang Tang , Feng Yuan , Yupeng Ren , Meihai Deng , Zhicheng Yao

{"title":"Exosome-derived hsa_circ_0007132 promotes lenvatinib resistance by inhibiting the ubiquitin-mediated degradation of NONO","authors":"Mingbo Cao , Yuxuan Li , Xiaorui Su , Yongchang Tang , Feng Yuan , Yupeng Ren , Meihai Deng , Zhicheng Yao","doi":"10.1016/j.ncrna.2025.05.007","DOIUrl":"10.1016/j.ncrna.2025.05.007","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) is a highly heterogeneous solid tumor, with its incidence showing a troubling upward trend over the past decade. Lenvatinib is one of the first-line medications for treating advanced HCC, however, the development of resistance significantly undermines its potential to improve patient prognosis. In recent years, exosomal circRNAs have been implicated in the resistance mechanisms of various cancers, yet their role in mediating lenvatinib resistance (LR) remains largely unexplored. In this study, we identified hsa_circ_0007132, which is upregulated in the serum exosomes of HCC patients exhibiting progressive disease (PD) following lenvatinib treatment. Subsequently, we employed LR cell lines to conduct both <em>in vitro</em> and <em>in vivo</em> experiments, which provided compelling evidence that hsa_circ_0007132 significantly promotes LR in HCC. Mechanistically, hsa_circ_0007132 interacts with the NONO protein, impairing its ubiquitination and leading to increased stability and upregulation of NONO expression, thereby enhancing NONO-mediated nuclear export of ZEB1 mRNA and elevating ZEB1 protein expression, which ultimately contributes to LR. In summary, our findings unveil a critical mechanism through which HCC mediates tumor progression and LR via exosomal hsa_circ_0007132, while also emphasizing that targeting NONO may represent a promising therapeutic strategy to overcome LR.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"14 ","pages":"Pages 1-13"},"PeriodicalIF":5.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Ocrelizumab in modulating gene and microRNA expression in multiple sclerosis Ocrelizumab在多发性硬化症中调节基因和microRNA表达的作用

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-15 DOI: 10.1016/j.ncrna.2025.05.009

Bruna De Felice , Elisabetta Signoriello , Concetta Montanino , Giuseppe Romano , Deborah Archetto , Elisabetta Maida , Martina Marciano , Simona Bonavita , Giacomo Lus , Federica Farinella , Cinzia Coppola

{"title":"Role of Ocrelizumab in modulating gene and microRNA expression in multiple sclerosis","authors":"Bruna De Felice , Elisabetta Signoriello , Concetta Montanino , Giuseppe Romano , Deborah Archetto , Elisabetta Maida , Martina Marciano , Simona Bonavita , Giacomo Lus , Federica Farinella , Cinzia Coppola","doi":"10.1016/j.ncrna.2025.05.009","DOIUrl":"10.1016/j.ncrna.2025.05.009","url":null,"abstract":"<div><div>Multiple sclerosis is an autoimmune neurodegenerative disease and one of the most significant challenges in modern neurology, impacting approximately 2.8 million people globally. As a multifactorial condition, susceptibility to MS can result from a combination of genetic and environmental factors. Current treatment strategies aim to prevent acute attacks, slow disease progression, and alleviate symptoms. Ocrelizumab, a monoclonal antibody targeting CD20, has demonstrated efficacy in clinical trials by reducing both disease activity and frequency of relapses.</div><div>Given the recent approval of this treatment, we investigated whether Ocrelizumab alters the expression of key miRNAs and genes involved in neuroinflammation, such as let-7a-5p, miR-14a-5p, miR-21a-5p, miR-338-3p, IL-1, IL-6, NEAT1, NEFL, NESTIN, SLC16A10 and TNF-alpha, by comparing their expression in patients’ blood before and after one year of treatment with Ocrelizumab. Additionally, we explored potential inverse correlations and direct or indirect interactions among the genes and miRNAs that showed significant changes in expression. Lastly, we conducted a pathway analysis to understand the overall effects potentially exerted by the drug.</div><div>Results revealed a significant decrease in the expression of TNF-alpha, SLC16A10, NEFL and IL-6, and an increase in let-7a-5p expression. There was an inverse correlation between let-7a-5p and the four genes, while the genes positively correlated with each other, suggesting let-7a-5p as a common modulator. These findings indicate that further investigation is needed to determine if the drug directly upregulates let-7a-5p, thereby downregulating the four genes, or if these expression changes are an indication of an overall reduction in inflammation.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 174-183"},"PeriodicalIF":5.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tongue squamous cell carcinoma-derived exosomes miR-21-5p affect tumor progression via promoting M2 macrophage polarization 舌鳞癌源性外泌体miR-21-5p通过促进M2巨噬细胞极化影响肿瘤进展

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-15 DOI: 10.1016/j.ncrna.2025.05.006

Zixian Xu , Xin Fang , Shan Wang , Jiabei Mu , Qixian Gai , Yantong Chen , Zheyi Sun , Jiemei Zhai

{"title":"Tongue squamous cell carcinoma-derived exosomes miR-21-5p affect tumor progression via promoting M2 macrophage polarization","authors":"Zixian Xu , Xin Fang , Shan Wang , Jiabei Mu , Qixian Gai , Yantong Chen , Zheyi Sun , Jiemei Zhai","doi":"10.1016/j.ncrna.2025.05.006","DOIUrl":"10.1016/j.ncrna.2025.05.006","url":null,"abstract":"<div><div>Exosome-mediated intercellular communication plays a key role in shaping the tumor microenvironment and promoting tumor progression. Recent studies have demonstrated that tumor exosomal miRNAs significantly contribute to the polarization of tumor-associated macrophages (TAMs). However, the molecular mechanisms underlying miRNA-mediated regulation of macrophage polarization by exosomes derived from tongue squamous cell carcinoma (TSCC) remain incompletely elucidated. In this study, small RNA sequencing analysis of exosomal miRNAs revealed miR-21-5p was highly expressed in TSCC-derived exosomes. Further investigation demonstrated a significant association between exosomal miR-21-5p and M2 polarization of tumor-associated macrophages (TAMs). Functionally, TSCC-derived exosomes promoted the polarization of M0 macrophages towards the M2 phenotype. Mechanistically, exosomal miR-21-5p enhanced M2 polarization of TAMs by inhibiting phosphorylation of ERK1/2. Additionally, we performed single-sample gene set enrichment analysis (ssGSEA), constructed a multivariate Cox regression model, and performed survival analysis using paired RNA transcriptome and clinical data from TSCC patients. Our results revealed a significant enrichment of M2 macrophages in the tumor microenvironment (TME) of TSCC compared to adjacent normal tissue. Furthermore, we confirmed that M2 macrophages infiltration is associated with poor prognosis in TSCC patients. In summary, our study demonstrates that TSCC-derived exosomal miR-21-5p plays an critical role in M2 macrophage polarization, and M2 macrophages infiltration contributes to the progression of TSCC. Therefore, these findings suggest that therapeutic targeting of miR-21-5p may represent a novel strategy for TSCC treatment by selectively modulating the M2 polarization of TAMs.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 184-196"},"PeriodicalIF":5.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of small nucleolar RNA retaining transcripts in human normal and cancer cells 人类正常细胞和癌细胞中小核仁RNA保留转录物的表征

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-09 DOI: 10.1016/j.ncrna.2025.05.004

Guglielmo Rambaldelli , Sidra Asghar , Giulia Venturi , Federico Zacchini , Margherita Serra , Catia Giovannini , Laura Gramantieri , Marco Bernini , Alberto Inga , Erik Dassi , Lorenzo Montanaro

{"title":"Characterization of small nucleolar RNA retaining transcripts in human normal and cancer cells","authors":"Guglielmo Rambaldelli , Sidra Asghar , Giulia Venturi , Federico Zacchini , Margherita Serra , Catia Giovannini , Laura Gramantieri , Marco Bernini , Alberto Inga , Erik Dassi , Lorenzo Montanaro","doi":"10.1016/j.ncrna.2025.05.004","DOIUrl":"10.1016/j.ncrna.2025.05.004","url":null,"abstract":"<div><div>Small nucleolar RNAs are non-coding RNAs typically encoded within the introns of both protein-coding and non-coding genes. Interestingly, a significant fraction of snoRNA sequences is found as retained introns of specific mRNA isoforms expressed from their host gene. In the present study, we aimed to define the representation of small nucleolar RNA retaining transcripts across various human cell types and tissues including cancer. We found that these type of transcripts are widely represented in normal tissues and cancer-derived cell lines, appearing both in their full-length form and, frequently, in a shorter variant. We characterized the shortening position, which occurs at or very close to the retained small nucleolar RNA sequence at the 5′ end. Interestingly, for some transcripts this shorter variant represents the only form detected. In addition, some of the small nucleolar RNA retaining transcripts can be localized into the cellular cytoplasmic fraction. Moreover, our findings point out that a variable but consistent proportion of small nucleolar RNA sequences in cells, tissues, and liquid biopsy samples is, in fact, present as small nucleolar RNA retaining transcripts, indicating that these elements should be carefully considered when snoRNA are evaluated as biomarkers. Considering that short reads and gene-based transcriptomic analysis completely overlooked these transcripts, potentially missing critical insights into their involvement in cancer and other diseases, our results strongly indicate that these type of transcripts should be further investigated in different contexts to better understand their biogenesis, sequence features, presence, and role within cells.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 153-161"},"PeriodicalIF":5.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal plasma microRNAs as potential biomarkers for triaging pregnancies of unknown location and ectopic pregnancy diagnosis 孕妇血浆microrna作为未知部位妊娠分类和异位妊娠诊断的潜在生物标志物

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-08 DOI: 10.1016/j.ncrna.2025.05.005

Christopher Kyriacou , Sung Hye Kim , Maria Arianoglou , Shabnam Bobdiwala , Margaret Pikovsky , Nina Parker , Jennifer Barcroft , Maya Al-Memar , Phillip R. Bennett , David A. MacIntyre , Tom Bourne , Vasso Terzidou

{"title":"Maternal plasma microRNAs as potential biomarkers for triaging pregnancies of unknown location and ectopic pregnancy diagnosis","authors":"Christopher Kyriacou , Sung Hye Kim , Maria Arianoglou , Shabnam Bobdiwala , Margaret Pikovsky , Nina Parker , Jennifer Barcroft , Maya Al-Memar , Phillip R. Bennett , David A. MacIntyre , Tom Bourne , Vasso Terzidou","doi":"10.1016/j.ncrna.2025.05.005","DOIUrl":"10.1016/j.ncrna.2025.05.005","url":null,"abstract":"<div><h3>Background</h3><div>Pregnancy of unknown location (PUL) is classified if an early pregnancy is not visualised on transvaginal ultrasonography (TVUS). Biomarkers currently used to triage PUL outcomes have varying accuracy. Delayed or missed diagnosis of ectopic pregnancies (EP) continue to cause significant morbidity and mortality. We investigated whether maternal plasma microRNAs (miRNAs) can predict and differentiate high-risk EP from viable (VIUP) or non-viable (NVIUP) intrauterine pregnancies.</div></div><div><h3>Methods</h3><div>Plasma was collected from women with PUL/EP (n = 120), mostly between four to eight weeks’ gestation, where outcomes of EP (n = 39), VIUP (n = 58) and NVIUP (miscarriage, n = 23) were determined using TVUS. Nanostring nCounter miRNA assay was used to examine the expression of ∼800 miRNAs in 22 women. Differentially expressed miRNAs were validated using RT-qPCR in 98 women.</div></div><div><h3>Results</h3><div>Nanostring nCounter miRNA assay identified 19 miRNAs which were expressed significantly higher in EP/NVIUP compared with VIUP. Two miRNAs were validated in a second, separate validation cohort using RT-qPCR: hsa-miR-21-5p in EP was 2.8-fold higher than in VIUP (<em>p</em> = 0.03, ROC AUC = 0.64), and hsa-miR-411-5p had 0.2-fold decreased expression (<em>p</em> = 0.02, ROC AUC = 0.66). Combining the divergent miRNAs as a ratio improved discrimination of EP from VIUP (<em>p</em> < 0.001, ROC AUC = 0.74).</div></div><div><h3>Conclusion</h3><div>Plasma miRNAs are differentially expressed in EP and VIUP and are detectable as early as four gestational weeks. Exploring miRNA targets may further understanding of EP pathophysiology, offering the potential to use miRNA as predictive and diagnostic markers in early pregnancy.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 162-173"},"PeriodicalIF":5.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ_0054633 silencing suppresses hyperglycemia-induced extracellular matrix accumulation in renal mesangial cells by regulating MiR-136-5p/SMAD3 signaling Circ_0054633沉默通过调节MiR-136-5p/SMAD3信号传导抑制高血糖诱导的肾系膜细胞细胞外基质积累

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-06 DOI: 10.1016/j.ncrna.2025.05.003

Qing Jiang , Hongxin Chen , Ke Wang , Xiaowei Zhu , Jun Yin , Wei Tang

{"title":"Circ_0054633 silencing suppresses hyperglycemia-induced extracellular matrix accumulation in renal mesangial cells by regulating MiR-136-5p/SMAD3 signaling","authors":"Qing Jiang , Hongxin Chen , Ke Wang , Xiaowei Zhu , Jun Yin , Wei Tang","doi":"10.1016/j.ncrna.2025.05.003","DOIUrl":"10.1016/j.ncrna.2025.05.003","url":null,"abstract":"<div><div>Diabetic kidney disease (DKD) is the most common complication of diabetes mellitus (DM), with excessive deposition of the extracellular matrix (ECM) produced by glomerular mesangial cells being its critical hallmark. circ_0054633, a circular RNA, is a promising biomarker for DM; however, the role of this circular RNA in DKD remains unknown. This study enrolled 10 healthy controls (CT), 10 DM patients, and 10 DKD patients, and serum circ_0054633 expression was analyzed. Circ_0054633 levels gradually increased in the CT, DM, and DKD groups, which also correlated with the serum indicator albumin/urine creatinine ratio and the degree of fibrosis. <em>In</em> <em>vitro</em> experiments, circ_0054633 silencing markedly attenuated hyperglycemia stress-induced cell proliferation and ECM accumulation in human renal mesangial cells). <em>In vivo</em> experiments indicated that circ_0054633 silencing in the db/db mouse kidney suppressed renal fibrosis. Mechanistically, circ_0054633 silencing exerted its effect by releasing miR-136-5p, which then downregulated SMAD3. The present study possibly demonstrates the clinical significance of circ_0054633, which may be a target for future DKD treatment.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 84-93"},"PeriodicalIF":5.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The emerging role of miRNAs in biological aging and age-related diseases mirna在生物衰老和年龄相关疾病中的新作用

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-05 DOI: 10.1016/j.ncrna.2025.05.002

Rawad Turko , Amro Hajja , Ahmad M. Magableh , Mohammed H. Omer , Areez Shafqat , Mohammad Imran Khan , Ahmed Yaqinuddin

{"title":"The emerging role of miRNAs in biological aging and age-related diseases","authors":"Rawad Turko , Amro Hajja , Ahmad M. Magableh , Mohammed H. Omer , Areez Shafqat , Mohammad Imran Khan , Ahmed Yaqinuddin","doi":"10.1016/j.ncrna.2025.05.002","DOIUrl":"10.1016/j.ncrna.2025.05.002","url":null,"abstract":"<div><div>Ageing is a complex biological process characterised by the accumulation of molecular and cellular damage leading to functional decline and an increased risk of chronic disease and geriatric syndromes. Despite data showing that lifestyle modifications, such as caloric restriction and exercise, can lead to healthy ageing and greatly reduce the incidence of chronic disorders, no medical therapies exist to delay or prevent these conditions effectively. We also lack tools to effectively track the ageing process in a manner that predicts an individual's risk of chronic disease and assess response to lifestyle or medical interventions. This review explores the emerging role of microRNAs (miRNAs), which are small non-coding RNAs that regulate gene expression, as a unifying mechanism underlying the biology of ageing and age-related conditions, including cardiovascular and neurodegenerative diseases, metabolic syndromes, musculoskeletal disorders such as sarcopenia, osteoarthritis and osteoporosis, and various cancers. We also examine the interactions of miRNAs with various hallmarks of ageing, such as DNA damage, cellular senescence, and mitochondrial dysfunction. We then explore the challenges of translating miRNA-based approaches from preclinical promise to clinical utility, emphasizing the need for trial-level validation to correlate miRNA profiles with clinically meaningful, patient-centred endpoints. By consolidating these findings, this article puts miRNAs forward as a pivotal mechanism in geroscience, offering a novel framework to mitigate ageing-related multimorbidity and bridge the gap between lifespan and healthspan.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 131-152"},"PeriodicalIF":5.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CircRNA-based AntimiR therapy: A novel approach to hypertension treatment 基于circrna的抗ir疗法：高血压治疗的新途径

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-05 DOI: 10.1016/j.ncrna.2025.05.001

Vahideh Tarhriz , Kamran Hosseini , Leila Abkhooie , Eric Lazartigues

{"title":"CircRNA-based AntimiR therapy: A novel approach to hypertension treatment","authors":"Vahideh Tarhriz , Kamran Hosseini , Leila Abkhooie , Eric Lazartigues","doi":"10.1016/j.ncrna.2025.05.001","DOIUrl":"10.1016/j.ncrna.2025.05.001","url":null,"abstract":"<div><div>The increasing recognition of the types and functions of non-coding RNAs has opened new avenues for their use as novel therapeutic strategies in the treatment of chronic diseases such as hypertension. While most preclinical and clinical studies on hypertension focus on prognosis and treatment, there are still no specific therapeutic approaches for primary and essential hypertension. Modest efficacy and reduced long-term adherence to treatment underscore the need for novel, mechanism-based strategies to address the underlying molecular mechanisms. AntimiRs, as synthetic RNA molecules, and circular RNAs (circRNAs), as naturally occurring RNA species that function as microRNA sponges, could play a crucial role in regulating the renin-angiotensin system (RAS). These non-coding RNAs hold significant potential as innovative approaches for managing primary and essential hypertension. This review aims to provide a comprehensive overview of circRNA-miRNA interactions involved in hypertension regulation via modulation of the RAS, their mechanisms of action, therapeutic advantages, and the major translational challenges, including delivery efficiency, off-target effects, and safety concerns. Optimization of the delivery systems, validating long-term efficacy, and navigating regulatory pathways to bring these promising ncRNA-based therapies closer to preclinical and clinical research of antimiR-based therapies are addressed. Furthermore, this review highlights the potential of these new RNA targets to fill the current therapeutic void and contribute to the advancement of precision medicine in hypertension and related cardiovascular diseases treatment.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 94-108"},"PeriodicalIF":5.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uremic toxins levels are associated with miR-223 in chronic kidney disease-associated anemia 慢性肾病相关性贫血患者尿毒症毒素水平与miR-223相关

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-05-02 DOI: 10.1016/j.ncrna.2025.04.009

Emma Brisot , Pierre-Marie Leprêtre , Eya Hamza , Ophélie Fourdinier , Benjamin Brigant , Hakim Ouled-Haddou , Gabriel Choukroun , Ziad A. Massy , Francis Verbeke , Valérie Metzinger-Le Meuth , Griet Glorieux , Laurent Metzinger

{"title":"Uremic toxins levels are associated with miR-223 in chronic kidney disease-associated anemia","authors":"Emma Brisot , Pierre-Marie Leprêtre , Eya Hamza , Ophélie Fourdinier , Benjamin Brigant , Hakim Ouled-Haddou , Gabriel Choukroun , Ziad A. Massy , Francis Verbeke , Valérie Metzinger-Le Meuth , Griet Glorieux , Laurent Metzinger","doi":"10.1016/j.ncrna.2025.04.009","DOIUrl":"10.1016/j.ncrna.2025.04.009","url":null,"abstract":"<div><div>Chronic kidney disease (CKD) poses a significant threat, with increased rates of cardiovascular and all-cause mortality. Anemia, common in CKD, is associated with accumulation of uremic toxins in the bloodstream. We previously demonstrated that the uremic toxin indoxyl sulfate (IS) impacts the regulation of erythropoiesis in cellular and preclinical CKD models. Here, the role of non-coding RNAs in this toxic effect was evaluated. The effect of IS on microRNA expression was measured in human erythropoietic cell line UT7/EPO, using nanostring. We found a significant increase of miR-223 in cells treated with IS. This finding was further validated in human primary CD34<sup>+</sup> cells, a more physiological model for human erythropoiesis. Finally, serum levels of miR-223 correlated with representative uremic toxins, including IS, in patients with various stages of CKD, and also with endothelial dysfunction markers, indicating a link with vascular damage. These correlations varied according to erythropoietin treatment and dialysis. These findings suggest that miR-223 may play a role in the development of anemia in CKD. Further investigation into the involvement of miR-223 in erythropoiesis is needed for a better understanding of the mechanisms underlying anemia in CKD and the potential role of uremic toxins. Ultimately, this may open up new therapeutic possibilities for the management of anemia in CKD.</div></div>","PeriodicalId":37653,"journal":{"name":"Non-coding RNA Research","volume":"13 ","pages":"Pages 121-130"},"PeriodicalIF":5.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

tsRNA, cell death and disease: Connecting the dots tsRNA，细胞死亡和疾病：连接点

IF 5.9 3区生物学

Non-coding RNA Research Pub Date : 2025-04-29 DOI: 10.1016/j.ncrna.2025.04.006

Xiaoyu Ma, Jiesi Xu, Guoping Li

引用次数: 0