IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-16 DOI: 10.1021/acs.est.4c09143

Hong Xu, Ping Gong, Xiaoping Wang, Lun Luo, Qianxue Yin, Xinyue Liu, Chuanfei Wang

{"title":"Release of Organochlorine Pollutants from Forest Fires: 1. Emission Factors and Revisiting Their Emissions in the Himalayan Regions","authors":"Hong Xu, Ping Gong, Xiaoping Wang, Lun Luo, Qianxue Yin, Xinyue Liu, Chuanfei Wang","doi":"10.1021/acs.est.4c09143","DOIUrl":"https://doi.org/10.1021/acs.est.4c09143","url":null,"abstract":"Worldwide forest fires have occurred frequently in recent years, a result of which may be the emission of so-called “legacy” organochlorine pollutants (OCPs) accumulated in forests. However, few studies have measured the emission factors (EFs) of the toxicity of the OCPs from forest fires. In this study, the EFs of vegetation burning were observed in forests along the altitudinal gradient from 1000 to 4200 m, and the EFs of ∑DDTs (dechlorodiphenylthrichloroethanes), HCB (hexachlorobenzene), ∑HCHs (hexachlorocyclohexanes), and ∑PCBs (polychlorinated biphenyls) were 2050 ± 1175, 379 ± 409, 48 ± 51, and 65 ± 59 ng/kg, respectively. Re-evaporation was the primary mechanism of the emission of OCP from forest fires. The masses of HCB, β-HCH, o,p′-DDD, p,p′-DDD, and PCB-28 in smoke increased 3–7 times compared with those in unburnt vegetation, suggesting the formation of these pollutants by the pyrolysis of biomass or other pollutants. Based on the observed EFs, previously estimated quantities of fire-emitted OCPs in the Himalayan regions were revisited. The DDT emissions from the Himalayan forest fires increased ∼70% compared with the previous estimation (from 19 to 32 kg/year). This highlighted that the EF observations could decrease the uncertainties of estimating OCP emissions from forest fires, which is helpful in revealing the potential roles of forest fires on global POP cycling.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"22 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Ventilated Three-Dimensional Artificial Lung System for Human Inhalation Exposure Studies 用于人体吸入暴露研究的通风三维人工肺系统

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-16 DOI: 10.1021/acs.est.4c08315

Haoxuan Chen, Airi Harui, Yu Feng, Liqiao Li, Saagar Patel, Jacob Schmidt, Michael D. Roth, Yifang Zhu

{"title":"A Ventilated Three-Dimensional Artificial Lung System for Human Inhalation Exposure Studies","authors":"Haoxuan Chen, Airi Harui, Yu Feng, Liqiao Li, Saagar Patel, Jacob Schmidt, Michael D. Roth, Yifang Zhu","doi":"10.1021/acs.est.4c08315","DOIUrl":"https://doi.org/10.1021/acs.est.4c08315","url":null,"abstract":"Traditional in vitro and in vivo models for inhalation toxicology studies often fail to replicate the anatomical and physiological conditions of the human lung. This limitation hinders our understanding of intrapulmonary exposures and their related health effects. To address this gap, we developed a ventilated artificial lung system that replicates human inhalation exposures in four key aspects: (1) facilitating continuous breathing with adjustable respiratory parameters; (2) distributing inhaled aerosols through transitional airflow fields in 3D-printed airway structures, which enables size-dependent particle deposition; (3) duplicating the warm and humid lung environment to promote inhaled aerosol dynamics, such as hygroscopic growth; and (4) supporting the cultivation of human airway epithelium for aerosol exposure and toxicological analyses. As a proof-of-concept application, we exposed human bronchial epithelial cells to electronic cigarette aerosols in the system. Our results show that electronic cigarette particles undergo significant hygroscopic growth within the artificial lung, leading to a 19% greater deposition dose compared to data collected at room temperature and relative humidity. Additionally, short-term exposure altered epithelial production of the chemokine Fractalkine in a nicotine-dependent manner, but no acute toxic effects were observed. This artificial lung system provides a more physiologically relevant in vitro model for studying inhalation exposures.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"23 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dominance of Plastic Emission in the High Arctic Aerosol in Light Spring 浅春季北极高纬度地区气溶胶中的塑料排放占主导地位

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-16 DOI: 10.1021/acs.est.4c06090

Jing Chen, Kimitaka Kawamura, Shao-Meng Li, Jan W. Bottenheim, Yiwen Zhang, Xin Huang, Cong-Qiang Liu, Pingqing Fu

{"title":"Dominance of Plastic Emission in the High Arctic Aerosol in Light Spring","authors":"Jing Chen, Kimitaka Kawamura, Shao-Meng Li, Jan W. Bottenheim, Yiwen Zhang, Xin Huang, Cong-Qiang Liu, Pingqing Fu","doi":"10.1021/acs.est.4c06090","DOIUrl":"https://doi.org/10.1021/acs.est.4c06090","url":null,"abstract":"Arctic haze has attracted considerable scientific interest for decades. However, limited studies have focused on the molecular composition of atmospheric particulate matter that contributes to Arctic haze. Our study collected atmospheric particles at Alert in the Canadian high Arctic from mid-February to early May 2000. Over 100 organic species were identified in the solvent-extractable fraction by gas chromatography–mass spectrometry, which were grouped by their functional groups. Plasticizer-derived phthalates were the most abundant, followed by polyacids, sugars, sugar alcohols, biogenic SOA tracers, and fossil fuel combustion tracers. During the dark winter, major contributors to Arctic aerosols include plastic emissions, biomass burning, secondary oxidation products, and fossil fuel combustion products. In the light spring, phthalates (58–76% of the identified organics) dominated, followed by microbial and marine sources and secondary oxidation products. By employing a tracer-based method, we discovered that naphthalene and sesquiterpene oxidation products were the major contributors to SOC, and these contributions were much higher in the winter than in the spring. However, monoterpene and isoprene oxidation products peaked in light spring. Our results confirm that organic aerosols in the Arctic atmosphere are dominated by anthropogenic sources, which consist of both long-range-transported particles and combustion-emitted organics, as well as aged anthropogenic secondary organic aerosols. Despite decreasing anthropogenic pollution being replaced by natural emissions, plastic-derived pollution, represented by phthalates, increased significantly in the high Arctic atmosphere after the polar sunrise.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"47 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perfluorohexanesulfonic Acid (PFHxS) Induces Hepatotoxicity through the PPAR Signaling Pathway in Larval Zebrafish (Danio rerio) 全氟己烷磺酸（PFHxS）通过 PPAR 信号通路诱导幼体斑马鱼（Danio rerio）产生肝毒性

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-16 DOI: 10.1021/acs.est.4c07056

Haolin Liao, Ying-Jie He, Shuwen Zhang, Xinyuan Kang, Xin Yang, Bentuo Xu, Jason T. Magnuson, Shuping Wang, Chunmiao Zheng, Wenhui Qiu

{"title":"Perfluorohexanesulfonic Acid (PFHxS) Induces Hepatotoxicity through the PPAR Signaling Pathway in Larval Zebrafish (Danio rerio)","authors":"Haolin Liao, Ying-Jie He, Shuwen Zhang, Xinyuan Kang, Xin Yang, Bentuo Xu, Jason T. Magnuson, Shuping Wang, Chunmiao Zheng, Wenhui Qiu","doi":"10.1021/acs.est.4c07056","DOIUrl":"https://doi.org/10.1021/acs.est.4c07056","url":null,"abstract":"In recent years, the industrial substitution of long-chain per- and polyfluoroalkyl substances (PFAS) with short-chain alternatives has become increasingly prevalent, resulting in the widespread environmental detection of perfluorohexanesulfonic acid (PFHxS), a short-chain PFAS. However, there remains limited information about the potential adverse effects of PFHxS at environmental concentrations to wildlife. Here, early life stage zebrafish (Danio rerio) were exposed to environmentally relevant concentrations of PFHxS to better characterize the adverse effects of PFHxS on aquatic organisms. Nontargeted, transcriptomic analysis revealed potential hepatotoxic effects in exposed larvae, including macrovesicular and microvesicular hepatic steatosis, as well as focal liver necrosis. Morphological, histological, biochemical, and targeted transcript expression profiles further confirmed significant alterations in hepatocellular lesion numbers, liver pathological structures, relative liver size, liver biochemical parameters, and liver function genes. To validate the PPAR-mediated toxicological mechanism identified as an enriched pathway through in silico bioinformatics analysis, we tested the coexposure to an antagonist and PPAR morpholino knockdown. This intervention alleviated PFHxS-induced hepatic effects, including reductions in the levels of aspartate aminotransferase, alanine aminotransferase, total cholesterol, and total triglycerides. Our results demonstrate that environmentally relevant concentrations of PFHxS can impair liver development and function in fish, which could have potential risks to aquatic organisms.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"250 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transfer Learning with a Graph Attention Network and Weighted Loss Function for Screening of Persistent, Bioaccumulative, Mobile, and Toxic Chemicals

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-16 DOI: 10.1021/acs.est.4c11085

Haobo Wang, Wenjia Liu, Jingwen Chen, Shengshe Ji

{"title":"Transfer Learning with a Graph Attention Network and Weighted Loss Function for Screening of Persistent, Bioaccumulative, Mobile, and Toxic Chemicals","authors":"Haobo Wang, Wenjia Liu, Jingwen Chen, Shengshe Ji","doi":"10.1021/acs.est.4c11085","DOIUrl":"https://doi.org/10.1021/acs.est.4c11085","url":null,"abstract":"In silico methods for screening hazardous chemicals are necessary for sound management. Persistent, bioaccumulative, mobile, and toxic (PBMT) chemicals persist in the environment and have high mobility in aquatic environments, posing risks to human and ecological health. However, lack of experimental data for the vast number of chemicals hinders identification of PBMT chemicals. Through an extensive search of measured chemical mobility data, as well as persistent, bioaccumulative, and toxic (PBT) chemical inventories, this study constructed comprehensive data sets on PBMT chemicals. To address the limited volume of the PBMT chemical data set, a transfer learning (TL) framework based on graph attention network (GAT) architecture was developed to construct models for screening PBMT chemicals, designating the PBT chemical inventories as source domains and the PBMT chemical data set as target domains. A weighted loss (LW) function was proposed and proved to mitigate the negative impact of imbalanced data on the model performance. Results indicate the TL-GAT models outperformed GAT models, along with large coverage of applicability domains and interpretability. The constructed models were employed to identify PBMT chemicals from inventories consisting of about 1 × 106 chemicals. The developed TL-GAT framework with the LW function holds broad applicability across diverse tasks, especially those involving small and imbalanced data sets.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"63 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics Signatures of Exposure to Ambient Air Pollution: A Large-Scale Metabolome-Wide Association Study in the Cancer Prevention Study-II Nutrition Cohort

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-16 DOI: 10.1021/acs.est.4c09592

Donghai Liang, Ziyin Tang, W. Ryan Diver, Jeremy A. Sarnat, Sabrina S. Chow, Haoran Cheng, Emily L. Deubler, Youran Tan, Stephanie M. Eick, Michael Jerrett, Michelle C. Turner, Ying Wang

{"title":"Metabolomics Signatures of Exposure to Ambient Air Pollution: A Large-Scale Metabolome-Wide Association Study in the Cancer Prevention Study-II Nutrition Cohort","authors":"Donghai Liang, Ziyin Tang, W. Ryan Diver, Jeremy A. Sarnat, Sabrina S. Chow, Haoran Cheng, Emily L. Deubler, Youran Tan, Stephanie M. Eick, Michael Jerrett, Michelle C. Turner, Ying Wang","doi":"10.1021/acs.est.4c09592","DOIUrl":"https://doi.org/10.1021/acs.est.4c09592","url":null,"abstract":"Existing air pollution metabolomics studies showed inconsistent results, often limited by small sample size and individual air pollutants effects. We conducted a metabolome-wide association study among 1096 women (68.2 ± 5.7 years) who provided blood samples (1998–2001) within the Cancer Prevention Study-II Nutrition Cohort. Annual average individual exposures to particulate matter, nitrogen dioxide, ozone, sulfur dioxide, and carbon monoxide in the year of blood draw were used. Metabolomics profiling was conducted on serum samples by Metabolon. We evaluated the individual air pollutants effects using multiple linear regression and the mixture effect using quantile g-computation, adjusting for confounders and false discovery rate (FDR). Ninety-five metabolites were significantly associated with at least one air pollutant or mixture (FDR < 0.05). These metabolites were enriched in pathways related to oxidative stress, systemic inflammation, energy metabolism, signals transduction, nucleic acid damage and repair, and xenobiotics. Sixty metabolites were confirmed with level 1 or 2 evidence, among which 21 have been previously linked to air pollution exposure, including taurine, creatinine, and sebacate. Overall, our results replicate prior findings in a large sample and provide novel insights into biological responses to long-term air pollution exposure using mixture analysis.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"250 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formation and Fate of Reactive Nitrogen during Biological Nitrogen Removal from Water: Important Yet Often Ignored Chemical Aspects of the Nitrogen Cycle

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-13 DOI: 10.1021/acs.est.4c03086

Qingxian Su, Carlos Domingo-Félez, Mei Zhi, Marlene Mark Jensen, Boyan Xu, How Yong Ng, Barth F. Smets

{"title":"Formation and Fate of Reactive Nitrogen during Biological Nitrogen Removal from Water: Important Yet Often Ignored Chemical Aspects of the Nitrogen Cycle","authors":"Qingxian Su, Carlos Domingo-Félez, Mei Zhi, Marlene Mark Jensen, Boyan Xu, How Yong Ng, Barth F. Smets","doi":"10.1021/acs.est.4c03086","DOIUrl":"https://doi.org/10.1021/acs.est.4c03086","url":null,"abstract":"Hydroxylamine, nitrous acid, and nitric oxide are obligate intermediates or side metabolites in different nitrogen-converting microorganisms. These compounds are unstable and susceptible to the formation of highly reactive nitrogen species, including nitrogen dioxide, dinitrogen trioxide, nitroxyl, and peroxynitrite. Due to the high reactivity and cytotoxicity, the buildup of reactive nitrogen can affect the interplay of microorganisms/microbial processes, stimulate the reactions with organic compounds like organic micropollutants (OMP) and act as the precursors of nitrous oxide (N2O). However, there is little understanding of the occurrence and significance of reactive nitrogen during biological nitrogen conversions in engineered water systems. In this review, we evaluate the formation and fate of reactive nitrogen produced by microorganisms involved in biological nitrogen removal (BNR) processes, i.e., nitritation/nitrification, denitratation/denitrification, anammox, and the combined processes. While the formation of reactive nitrogen intermediates is entirely controlled by microbial activities, the consumption can be either biological or purely chemical. Changes in environmental conditions, such as redox transition, pH, and substrate availability, can imbalance the production and consumption of these reactive intermediates, thus leading to the transient accumulation of species. Based on previous experimental evidence, environmental relevance of reactive nitrogen in BNR systems, particularly related to abiotic N2O production and OMP transformation, is demonstrated.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"29 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of Aftertreatment Technologies to Alleviating SOA and Toxicity Generation from Typical Diesel Engine-Emitted I/SVOCs

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-13 DOI: 10.1021/acs.est.4c09555

Yaoqiang Huo, Jianguo Liu, Di Wu, Yuankai Shao, Xiwen Song, Zihua Guo, Anlin Liu, Qing Li, Jianmin Chen

{"title":"Contribution of Aftertreatment Technologies to Alleviating SOA and Toxicity Generation from Typical Diesel Engine-Emitted I/SVOCs","authors":"Yaoqiang Huo, Jianguo Liu, Di Wu, Yuankai Shao, Xiwen Song, Zihua Guo, Anlin Liu, Qing Li, Jianmin Chen","doi":"10.1021/acs.est.4c09555","DOIUrl":"https://doi.org/10.1021/acs.est.4c09555","url":null,"abstract":"The removal capacity of aftertreatment technologies equipped on diesel exhaust in intermediate and semivolatile organic compounds (I/SVOCs) remains unclear. This study quantified the effect of typical aftertreatment technologies (China VI) on diesel engine-emitted I/SVOCs, related secondary organic aerosol (SOA), and toxic effects. The equipped aftertreatment devices could mitigate the emission factors (EFs) by 70.8 ± 3.4 to 82.5 ± 20.9% for I/SVOCs, 72.7 ± 18.6 to 77.5 ± 4.2% for SOA production, and 75.7 ± 9.3 to 82.4 ± 9.2% for toxic equivalent quantity (TEQ). Aftertreatment units are better for removing alkanes, benzenes, and ketones. The TEQ from the cold-start cycle is 1.3- to 5.7-fold higher than that from the hot-start cycle. In contrast, the EFs of ship-emitted I/SVOCs are 3.7- to 5.3-fold higher than those of diesel vehicles equipped with an aftertreatment system when burning the same fuel, leading to 2.1- to 3.0-fold higher SOA production and 3.1- to 6.7-fold TEQ. Implementation of control devices on marine diesel engines could reduce I/SVOC EFs, SOA production, and eye irritation TEQ of ships by 76.7 ± 12.2, 75.1 ± 12.2, and 79.1 ± 9.6%, respectively. These results indicate that it is vital to equip marine engines with aftertreatment technologies to improve air quality and protect human health.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"29 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Environmental Conditions on the Escape Rates of Biocontained Genetically Engineered Microbes

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-13 DOI: 10.1021/acs.est.4c10893

Anna M. Hartig, Wentao Dai, Ke Zhang, Krisha Kapoor, Austin G. Rottinghaus, Tae Seok Moon, Kimberly M. Parker

{"title":"Influence of Environmental Conditions on the Escape Rates of Biocontained Genetically Engineered Microbes","authors":"Anna M. Hartig, Wentao Dai, Ke Zhang, Krisha Kapoor, Austin G. Rottinghaus, Tae Seok Moon, Kimberly M. Parker","doi":"10.1021/acs.est.4c10893","DOIUrl":"https://doi.org/10.1021/acs.est.4c10893","url":null,"abstract":"The development of genetically engineered microbes (GEMs) has resulted in an urgent need to control their persistence in the environment. The use of biocontainment such as kill switches is a critical approach to prevent the unintended proliferation of GEMs; however, the effectiveness of kill switches─reported as escape rates, i.e., the ratio of the number of viable microbes when the kill switch is triggered relative to the number when it is not triggered─is typically assessed under laboratory conditions that do not resemble environmental conditions under which biocontainment must perform. In this study, we discovered that the escape rate of an Escherichia coli GEM biocontained with a CRISPR-based kill switch triggered by anhydrotetracycline (aTc) increased by 3–4 orders of magnitude when deployed in natural surface waters as compared to rich laboratory media. We identified that environmental conditions (e.g., pH, nutrient levels) may contribute to elevated escape rates in multiple ways, including by altering the chemical speciation of the kill switch trigger to reduce its uptake and providing limited nutrients required for the kill switch to function. Our study demonstrated that conditions in the intended environment must be considered in order to design effective GEM biocontainment strategies.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"7 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning-Assisted “Shrink-Restricted” SERS Strategy for Classification of Environmental Nanoplastic-Induced Cell Death

IF 9.028 1区环境科学与生态学

环境科学与技术 Pub Date : 2024-12-13 DOI: 10.1021/acs.est.4c05590

Ruili Li, Xiaotong Sun, Yuyang Hu, Shenghong Liu, Shuting Huang, Zhipeng Zhang, Kecen Chen, Qi Liu, Xiaoqing Chen

{"title":"Machine Learning-Assisted “Shrink-Restricted” SERS Strategy for Classification of Environmental Nanoplastic-Induced Cell Death","authors":"Ruili Li, Xiaotong Sun, Yuyang Hu, Shenghong Liu, Shuting Huang, Zhipeng Zhang, Kecen Chen, Qi Liu, Xiaoqing Chen","doi":"10.1021/acs.est.4c05590","DOIUrl":"https://doi.org/10.1021/acs.est.4c05590","url":null,"abstract":"The biotoxicity of nanoplastics (NPs), especially from environmental sources, and “NPs carrier effect” are in the early stages of research. This study presents a machine learning-assisted “shrink-restricted” SERS strategy (SRSS) to monitor molecular changes in the cellular secretome exposure to six types of NPs. Utilizing three-dimensional (3D) Ag@hydrogel-based SRSS, active targeting of molecules within adjustable nanogaps was achieved to track information. Machine learning was employed to analyze the overall spectral profiles, biochemical signatures, and time-dependent changes. Results indicate that environmentally derived NPs exhibited higher toxicity to BEAS-2B and L02 cells. Notably, the “NPs carrier effect,” resulting from pollutant adsorption, proved to be more harmful. This effect altered the death pathway of BEAS-2B cells from a combination of apoptosis and ferroptosis to primarily ferroptosis. Furthermore, L02 cells demonstrated greater metabolic vulnerability to NPs exposure than that of BEAS-2B cells, especially concerning the “NPs carrier effect.” Traditional detection methods for cell death often rely on end point assays, which limit temporal resolution and focus on single or multiple markers. In contrast, our study pioneers a machine learning-assisted SERS approach for monitoring overall metabolic levels post-NPs exposure at both cellular and molecular levels. This endeavor has significantly advanced our understanding of the risks associated with plastic pollution.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"8 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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