{"title":"Environmentally Relevant Concentrations of <i>S</i>-6PPD-Quinone Caused More Serious Hepatotoxicity Than <i>R</i>-Enantiomer and Racemate in <i>Oncorhynchus mykiss</i>.","authors":"Shanshan Di, Haigui Xu, Yundong Yu, Peipei Qi, Zhiwei Wang, Zhenzhen Liu, Huiyu Zhao, Yuanxiang Jin, Xinquan Wang","doi":"10.1021/acs.est.4c06357","DOIUrl":"https://doi.org/10.1021/acs.est.4c06357","url":null,"abstract":"<p><p>6PPD-quinone (6PPD-Q) is frequently detected in various environmental media, and the environmentally relevant concentrations can be fatal to <i>Oncorhynchus mykiss</i>. Notably, 6PPD-Q has two enantiomers (<i>S</i>-6PPD-Q and <i>R</i>-6PPD-Q). In this study, <i>O. mykiss</i> was separately exposed to each enantiomer and racemate of 6PPD-Q for 96 h at environmentally relevant concentrations, and livers were collected. Effects on the biochemical, pathological, and ultrastructural changes were assessed, and metabolomics was conducted to elucidate the potential hepatotoxicity mechanism. Compared with the control treatment, the levels of catalase (CAT, all treatments except for 0.1 μg/L <i>rac</i>-6PPD-Q), and glutathione-S-transferase (GST, all treatments) significantly declined. Hepatocyte space became smaller, nuclear morphology changed, and nucleolysis occurred. Mitochondrial malformation and vesicle-like structure dilation of the endoplasmic reticulum (ER) were observed in the hepatocytes, which was most serious after <i>S</i>-6PPD-Q exposure. Some amino acid metabolism, folate biosynthesis, taurine and hypotaurine metabolism and purine metabolism were disturbed, consistent with mitochondrial dysfunction and ER stress. The differential metabolites were in the order of <i>S</i>-6PPD-Q (216) > <i>rac</i>-6PPD-Q (88) > <i>R</i>-6PPD-Q (56). Thus, 6PPD-Q-induced hepatic mitochondrial dysfunction and ER stress, causing metabolic disturbance and oxidative stress might be the toxic mechanism of 6PPD-Q in <i>O. mykiss</i> liver, and <i>S</i>-6PPD-Q effects were the most serious.</p>","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":10.8,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-21DOI: 10.1021/acs.est.4c06225
Yukui Gou, Yuchen Yang, Weiwei Zheng, Xuzheng Ji, Nan Lu, Wenqing Wang, Meiyan Zhong, Yongqian Shi, Jianying Huang, Weilong Cai, Yuekun Lai
{"title":"Fluorine-Free Amphiphobic SBS/PAN Micro/Nanofiber Membrane by Integrating Click Reaction with Electrospinning for Efficient and Recyclable Air Filtration","authors":"Yukui Gou, Yuchen Yang, Weiwei Zheng, Xuzheng Ji, Nan Lu, Wenqing Wang, Meiyan Zhong, Yongqian Shi, Jianying Huang, Weilong Cai, Yuekun Lai","doi":"10.1021/acs.est.4c06225","DOIUrl":"https://doi.org/10.1021/acs.est.4c06225","url":null,"abstract":"The membrane fouling derived from the accumulated dust pollutants and highly viscous oily particles causes irreversible damage to the filtration performance of air filters and results in a significant reduction in their service life. However, it is still challenging to construct high-efficiency and antifouling air filtration membranes with recyclable regeneration. Herein, the fluorine-free amphiphobic micro/nanofiber composite membrane was controllably constructed by integrating click chemistry reaction and electrospinning technique. Low-surface-energy fibers were constructed by a thiol–ene click chemical reaction between mercaptosilane and vinyl groups of polystyrene–butadiene-styrene (SBS), combined with hydroxyl-terminated poly(dimethylsiloxane) during the electrospinning process. The functional air filter is then prepared by the two-layer composite strategy. Because of the advantages of liquid-like fibrous surface and micro/nanofibrous porous structure, SBS/PAN composite membrane simultaneously shows superior antifouling performances of pollutants and filtration efficiency of over 97% PM<sub>0.3</sub> removal. More importantly, the antifouling fibrous membrane still presents a stable and efficient filtration efficiency after multiple washes. Its service life in dust filtration environments is approximately 1.7 times longer than that of the substrate membrane. This work may provide a significant reference for the design of antifouling fiber membranes and high-efficiency air filters with long life spans and reusability.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-20DOI: 10.1021/acs.est.4c05816
Yizhu Chen, Zhenyu Wang, Zhengyang Fang, Chengpeng Huang, Han Xu, Huanhuan Zhang, Tianyu Zhang, Fu Wang, Lan Luo, Guoliang Shi, Xinming Wang, Mingjin Tang
{"title":"Dominant Contribution of Non-dust Primary Emissions and Secondary Processes to Dissolved Aerosol Iron","authors":"Yizhu Chen, Zhenyu Wang, Zhengyang Fang, Chengpeng Huang, Han Xu, Huanhuan Zhang, Tianyu Zhang, Fu Wang, Lan Luo, Guoliang Shi, Xinming Wang, Mingjin Tang","doi":"10.1021/acs.est.4c05816","DOIUrl":"https://doi.org/10.1021/acs.est.4c05816","url":null,"abstract":"Solubility largely determines the impacts of aerosol Fe on marine ecosystems and human health. Currently, modeling studies have large uncertainties in aerosol Fe solubility due to inadequate understanding of the sources of dissolved Fe. This work investigated seasonal variations of Fe solubility in coarse and fine aerosols in Qingdao, a coastal city in the Northwest Pacific, and utilized a receptor model for source apportionment of total and dissolved aerosol Fe. Desert dust was found to be the main source of total Fe, contributing 65 and 81% annually to total Fe in coarse and fine particles, respectively; in contrast, dissolved aerosol Fe originated primarily from combustion, industrial, and secondary sources. The annual average contributions to dissolved Fe in coarse and fine particles were 68 and 47% for the secondary source and 32 and 33% for the combustion source, respectively. Aerosol Fe solubility was found to be highest in summer and lowest in spring, showing seasonal patterns similar to those of aerosol acidity. Increase in Fe solubility in atmospheric particles, when compared to desert dust, was mainly caused by secondary processing and combustion emission, and the effect of secondary processes was dictated by aerosol acidity and liquid water content.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-19DOI: 10.1021/acs.est.4c02833
Pilleriin Peets, May Britt Rian, Jonathan W. Martin, Anneli Kruve
{"title":"Evaluation of Nontargeted Mass Spectral Data Acquisition Strategies for Water Analysis and Toxicity-Based Feature Prioritization by MS2Tox","authors":"Pilleriin Peets, May Britt Rian, Jonathan W. Martin, Anneli Kruve","doi":"10.1021/acs.est.4c02833","DOIUrl":"https://doi.org/10.1021/acs.est.4c02833","url":null,"abstract":"The machine-learning tool MS2Tox can prioritize hazardous nontargeted molecular features in environmental waters, by predicting acute fish lethality of unknown molecules based on their MS<sup>2</sup> spectra, prior to structural annotation. It has yet to be investigated how the extent of molecular coverage, MS<sup>2</sup> spectra quality, and toxicity prediction confidence depend on sample complexity and MS<sup>2</sup> data acquisition strategies. We compared two common nontargeted MS<sup>2</sup> acquisition strategies with liquid chromatography high-resolution mass spectrometry for structural annotation accuracy by SIRIUS+CSI:FingerID and MS2Tox toxicity prediction of 191 reference chemicals spiked to LC-MS water, groundwater, surface water, and wastewater. Data-dependent acquisition (DDA) resulted in higher rates (19–62%) of correct structural annotations among reference chemicals in all matrices except wastewaters, compared to data-independent acquisition (DIA, 19–50%). However, DIA resulted in higher MS<sup>2</sup> detection rates (59–84% DIA, 37–82% DDA), leading to higher true positive rates for spectral library matching, 40–73% compared to 34–72%. DDA resulted in higher MS2Tox toxicity prediction accuracy than DIA, with root-mean-square errors of 0.62 and 0.71 log-mM, respectively. Given the importance of MS<sup>2</sup> spectral quality, we introduce a “CombinedConfidence” score to convey relative confidence in MS2Tox predictions and apply this approach to prioritize potentially ecotoxic nontargeted features in environmental waters.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-19DOI: 10.1021/acs.est.4c05523
Yulong Wang, Xiaoxiao Li, Shijie Chen, Ji Yang, Bo Fang, Hao Chen, Yiming Yao, Hongwen Sun
{"title":"Structure-Dependent Distribution, Metabolism, and Toxicity Effects of Alkyl Organophosphate Esters in Lettuce (Lactuca sativa L.)","authors":"Yulong Wang, Xiaoxiao Li, Shijie Chen, Ji Yang, Bo Fang, Hao Chen, Yiming Yao, Hongwen Sun","doi":"10.1021/acs.est.4c05523","DOIUrl":"https://doi.org/10.1021/acs.est.4c05523","url":null,"abstract":"This study provides a comprehensive investigation into the structure-dependent uptake, distribution, biotransformation, and potential toxicity effects of alkyl organophosphate esters (OPEs) in hydroponic lettuce (<i>Lactuca sativa</i> L.). Trimethyl, triethyl, and tripropyl phosphates were readily absorbed and acropetally translocated, while tributyl, tripentyl, and trihexyl phosphates accumulated mainly in lateral roots. The acropetal translocation potential was negatively associated with log <i>K</i><sub>ow</sub> values. Trimethyl and triethyl phosphates are less prone to biotransformation, while a total of 14 novel hydrolysis, hydroxylated, and conjugated metabolites were identified for other OPEs using nontarget analysis. The extent of hydroxylation decreases from tripropyl phosphate to trihexyl phosphate, but multiple hydroxylations occurred more frequently on longer chain OPEs. Further comparative toxicity test revealed that hydrolyzed and hydroxylated metabolites have stronger toxic effects on Ca<sup>2+</sup>-dependent protein kinases (CDPK) than their parent OPEs. Dibutyl 3-hydroxybutyl phosphate particularly induces upregulation of CDPK in lateral roots of lettuce, probably associated with adenine reduction that may play an important role in the self-defense and detoxification processes. This study contributes to understanding the uptake and transformation behaviors of alkyl OPEs as well as their associations with a toxic effect on lettuce. This emphasizes the necessary evaluation of the environmental risk of the use of OPEs, particularly focusing on their hydroxylated metabolites.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-19DOI: 10.1021/acs.est.4c05434
Zachary T. Kralles, Prashant K. Deherikar, Christian A. Werner, Ximin Hu, Edward P. Kolodziej, Ning Dai
{"title":"Halogenation of Anilines: Formation of Haloacetonitriles and Large-Molecule Disinfection Byproducts","authors":"Zachary T. Kralles, Prashant K. Deherikar, Christian A. Werner, Ximin Hu, Edward P. Kolodziej, Ning Dai","doi":"10.1021/acs.est.4c05434","DOIUrl":"https://doi.org/10.1021/acs.est.4c05434","url":null,"abstract":"Aniline-related structures are common in anthropogenic chemicals, such as pharmaceuticals and pesticides. Compared with the widely studied phenolic compounds, anilines have received far less assessment of their disinfection byproduct (DBP) formation potential, even though anilines and phenols likely exhibit similar reactivities on their respective aromatic rings. In this study, a suite of 19 aniline compounds with varying <i>N</i>- and ring-substitutions were evaluated for their formation potentials of haloacetonitriles and trihalomethanes under free chlorination and free bromination conditions. Eight of the aniline compounds formed dichloroacetonitrile at yields above 0.50%; the highest yields were observed for 4-nitroaniline, 3-chloroaniline, and 4-(methylsulfonyl)aniline (1.6–2.3%). Free bromination generally resulted in greater haloacetonitrile yields with the highest yield observed for 2-ethylaniline (6.5%). The trihalomethane yields of anilines correlated with their haloacetonitrile yields. Product analysis of aniline chlorination by liquid chromatography–high-resolution mass spectrometry revealed several large-molecule DBPs, including chloroanilines, (chloro)hydroxyanilines, (chloro)benzoquinone imines, and ring-cleavage products. The product time profiles suggested that the reaction pathways include initial ring chlorination and hydroxylation, followed by the formation of benzoquinone imines that eventually led to ring cleavage. This work revealed the potential of aniline-related moieties in micropollutants as potent precursors to haloacetonitriles and other emerging large-molecule DBPs with the expected toxicity.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142246157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-19DOI: 10.1021/acs.est.4c01859
Mathis Loïc Messager,Hervé Pella,Thibault Datry
{"title":"Inconsistent Regulatory Mapping Quietly Threatens Rivers and Streams.","authors":"Mathis Loïc Messager,Hervé Pella,Thibault Datry","doi":"10.1021/acs.est.4c01859","DOIUrl":"https://doi.org/10.1021/acs.est.4c01859","url":null,"abstract":"Even the most stringent environmental law cannot protect a river if its tributaries remain exposed to pollution and other threats upstream. Excluding a subset of watercourses from legal protection therefore threatens to alter freshwater ecosystems across entire river networks and the services they provide, such as drinking water and flood regulation. Considerable attention has been devoted to defining the scope of environmental laws protecting watercourses. Yet how these definitions are implemented through regulatory mapping, the cartography of waterbodies that legally qualify as watercourses and are thus protected, has not been examined outside of the United States. Here, we demonstrate the consequences of regulatory mapping on the extent of river networks that are protected, using France as a case study. By assembling the first map of France's watercourses protected under the Water Law, we estimate that a quarter of previously mapped hydrographic segments were excluded from protection and found stark geographical variations in the extent of protected ecosystems. Headwater and nonperennial segments are disproportionately excluded by 28% compared to their prevalence (67%) in the overall hydrographic network, with potentially far-reaching implications for biodiversity and people. We expect regulatory frameworks in most countries to be equally susceptible to local interpretation of legal definitions.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142246982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Indigenized Characterization Factors for Health Damage Due to Ambient PM2.5 in Life Cycle Impact Assessment in China","authors":"Qiao Ma, Renxiao Yuan, Shan Wang, Yuchen Sun, Qianqian Zhang, Xueliang Yuan, Qingsong Wang, Congwei Luo","doi":"10.1021/acs.est.3c08122","DOIUrl":"https://doi.org/10.1021/acs.est.3c08122","url":null,"abstract":"Life cycle assessment (LCA) is a broadly used method for quantifying environmental impacts, and life cycle impact assessment (LCIA) is an important step as well as a major source of uncertainties in LCA. Characterization factors (CFs) are pivotal elements in LCIA models. In China, the health loss due to ambient PM<sub>2.5</sub> is an important aspect of LCIA results, which, however, is generally assessed by adopting CFs developed by global models and there remains a need to integrate localized considerations and the latest information for more precise applications in China. In this study, we developed indigenized CFs for LCIA of health damage due to ambient PM<sub>2.5</sub> in China by coupling the atmospheric chemical transport model GEOS-Chem, exposure–response model GEMM containing Chinese cohort studies, and the latest local data. Results show that CFs of four major PM<sub>2.5</sub> precursors all exhibit significant interregional variation and monthly differences in China. Our results were generally an order of magnitude higher and show disparate spatial distribution compared to CFs currently in use, suggesting that the health damage due to ambient PM<sub>2.5</sub> was underestimated in LCIA in China, and indigenized CFs need to be adopted for more accurate results in LCIA and LCA studies.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
环境科学与技术Pub Date : 2024-09-19DOI: 10.1021/acs.est.4c05558
Jing Duan, Ru-Jin Huang, Chunshui Lin, Jincan Shen, Lu Yang, Wei Yuan, Ying Wang, Yi Liu, Wei Xu
{"title":"Aromatic Nitration Enhances Absorption of Biomass Burning Brown Carbon in an Oxidizing Urban Environment","authors":"Jing Duan, Ru-Jin Huang, Chunshui Lin, Jincan Shen, Lu Yang, Wei Yuan, Ying Wang, Yi Liu, Wei Xu","doi":"10.1021/acs.est.4c05558","DOIUrl":"https://doi.org/10.1021/acs.est.4c05558","url":null,"abstract":"Brown carbon (BrC) from biomass burning constitutes a significant portion of light-absorbing components in the atmosphere. Although the aging of BrC surrogates from biomass burning has been studied in many laboratory settings, BrC aging behavior in real-world urban environments is not well understood. In this study, through a combination of online dynamic monitoring and offline molecular characterization, the ambient optical aging of BrC was linked to its dynamic changes in molecular composition. Enhanced light absorption by BrC was consistently observed during the periods dominated by oxygenated biomass burning organic aerosol (BBOA), in contrast to periods dominated by primary emissions or secondary formation in aqueous-phase. This enhancement was linked to the formation of nitrogen-containing compounds during the ambient aging of BBOA. Detailed molecular characterization, alongside analysis of environmental parameters, revealed that an increased atmospheric oxidizing capacity, marked by elevated levels of ozone and nighttime NO<sub>3</sub> radicals, facilitated the formation of nitrated aromatic BrC chromophores. These chromophores were primarily responsible for the enhanced light absorption during the ambient aging of BBOA. This study elucidates the nitration processes that enhance BrC light absorption for ambient BBOA, and highlights the crucial role of meteorological conditions. Furthermore, our findings shed light on the chemical and optical aging processes of biomass burning BrC in ambient air, offering insights into its environmental behavior and effects.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Short Chain Chlorinated Paraffins Impaired Spermatogenesis Process in Mice via Inhibiting α-KG/TET Enzyme Activity","authors":"Ruiyang Meng, Xingde Du, Yu Fu, Fufang Wang, Yingfei Yang, Feiyang Guo, Xin Wang, Kangfeng Ge, Jun Yang, Xiao Liang, Hongxiang Guo, Wenjun Wang, Xiaodong Liu, Huizhen Zhang","doi":"10.1021/acs.est.4c05895","DOIUrl":"https://doi.org/10.1021/acs.est.4c05895","url":null,"abstract":"Short chain chlorinated paraffins (SCCPs) are widely found in various environmental media and potentially threaten human health. However, the toxicity mechanisms of SCCPs to the male reproductive system remain unclear. In this study, male BALB/c mice and GC-1 cells were used to investigate the reproductive toxicity of SCCPs and their molecular mechanisms. SCCPs decreased the content of the tricarboxylic acid cycle intermediate α-KG in testicular cells, thus inhibiting the activity of the DNA demethylase TET enzyme and resulting in an increase in the overall methylation level of the testicular genome. Correspondingly, the promoter demethylation and expression of spermatogenesis-related genes <i>Rbm46</i>, <i>Sohlh1</i>, <i>Kit</i>, and <i>Dmrt1</i> were significantly reduced by SCCPs, which further prevented the transformation of spermatogonia to spermatocytes and reduced sperm quality in mice. The <i>in vitro</i> experiments suggested that the TGFβ pathway activated by oxidative stress might be an essential reason for inhibiting the tricarboxylic acid cycle and the reduction of α-KG content in testicular cells induced by SCCPs. Overall, this study reveals a novel metabolic regulatory mechanism of SCCPs-induced spermatogenesis disorders, which provides an essential theoretical basis for the prevention of reproductive toxicity of SCCPs.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":9.028,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142246158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}