Mexican Journal of Biotechnology最新文献_第6页

Characterization of the degradation potential of xenobiotic compounds by the rhizobacteria Azospirillum brasilense 巴西固氮螺旋菌对外生化合物降解潜力的研究

Mexican Journal of Biotechnology Pub Date : 2019-01-01 DOI: 10.29267/mxjb.2019.4.2.10

M. A. Cruz-Hernández, J. M. Jiménez-Andrade, A. M. Herrera

{"title":"Characterization of the degradation potential of xenobiotic compounds by the rhizobacteria Azospirillum brasilense","authors":"M. A. Cruz-Hernández, J. M. Jiménez-Andrade, A. M. Herrera","doi":"10.29267/mxjb.2019.4.2.10","DOIUrl":"https://doi.org/10.29267/mxjb.2019.4.2.10","url":null,"abstract":"La contaminacion ocasionada por hidrocarburos representa un riesgo para el medioambiente. Se han enfocado estudios en la busqueda de alternativas para contribuir a revertir su efecto. Una de ellas involucra el uso de microorganismos nativos capaces de persistir en ambientes contaminados. Por lo cual el objetivo de este trabajo fue analizar el potencial de biotipos de Azospirillum brasilense para degradar compuestos xenobioticos como fenantreno, xileno, tolueno y naftaleno. Se caracterizo la produccion de biosurfactantes, se evaluo in vitro la capacidad de tolerancia a hidrocarburos derivados del petroleo y busqueda de genes relacionados con la degradacion de compuestos aromaticos mediante el programa RAST. Los resultados mostraron que los biotipos produjeron biosurfactantes y se seleccionaron cinco de ellos para realizar ensayos de tolerancia a xileno, tolueno, fenantreno y naftaleno; en los cuales no se observo crecimiento bacteriano despues de 216 horas de incubacion a 30 °C. Se registraron 19 secuencias codificantes relacionadas con la degradacion de compuestos aromaticos; de las cuales 11 estan asociadas con el metabolismo de los compuestos intermedios aromaticos centrales y 5 con las vias catabolicas perifericas cuya funcion se asocia con las rutas de degradacion de quinato, benzoato, salicilato, gentisato y tolueno","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"4 1","pages":"10-22"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Neurospora sitophila crecido en medios adicionados con dibutil ftalato en fermentación sumergida: Cinética de crecimiento y actividad de esterasa 在添加邻苯二甲酸二丁酯的培养基中发酵生长嗜锡孢菌:生长动力学和酯酶活性

Mexican Journal of Biotechnology Pub Date : 2019-01-01 DOI: 10.29267/mxjb.2019.4.1.61

Dania Fernanda Sánchez-Sánchez, C. Sánchez

{"title":"Neurospora sitophila crecido en medios adicionados con dibutil ftalato en fermentación sumergida: Cinética de crecimiento y actividad de esterasa","authors":"Dania Fernanda Sánchez-Sánchez, C. Sánchez","doi":"10.29267/mxjb.2019.4.1.61","DOIUrl":"https://doi.org/10.29267/mxjb.2019.4.1.61","url":null,"abstract":"Los ésteres de ftalato son compuestos usados principalmente como plastificantes y aditivos para proveer de flexibilidad y maleabilidad a los productos plásticos. Dentro de los ftalatos más empleados se encuentra el dibutil ftalato (DBF), el cual se utiliza como un aditivo en adhesivos, tintas para impresoras y en productos cosméticos. En este trabajo se evaluaron la velocidad específica de crecimiento (μ), biomasa máxima (Xmax), pH, consumo de glucosa y actividad específica de esterasas de Neurospora sitophila en medios adicionados con diferentes concentraciones de DBF (1500 y 2000 mg/L) en fermentación sumergida. N. sitophila presentó una mayor velocidad específica de crecimiento en el medio adicionado con 1500 mg de DBF/L que en el medio adicionado con 2000 mg de DBF/L. El medio adicionado con 2000 mg de DBF/L presentó la mayor producción de biomasa. La mayor actividad específica de esterasas fue observada en el medio conteniendo 1500 mg de DBF/L. Esta investigación sugiere que N. sitophila es capaz de emplear altas concentraciones de DBF como fuente de carbono y energía.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation 邻苯二甲酸二(2-乙基己基)液体发酵中镰刀菌的生长和酯酶活性

Mexican Journal of Biotechnology Pub Date : 2019-01-01 DOI: 10.29267/MXJB.2019.4.1.51

Naomi Surellem Ríos-González, Ángel González-Márquez, C. Sánchez

{"title":"Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation","authors":"Naomi Surellem Ríos-González, Ángel González-Márquez, C. Sánchez","doi":"10.29267/MXJB.2019.4.1.51","DOIUrl":"https://doi.org/10.29267/MXJB.2019.4.1.51","url":null,"abstract":"Di (2-ethyl hexyl) phthalate (DEHP) is a plasticizer present in various products, mainly those made with PVC. This phthalate has attracted attention due to its important participation in the contamination of the environment. It has been reported as an endocrine-disrupting compound in mammals. Fusarium culmorum is a phytopathogenic fungus able to degrade DEHP, because it produces esterases, which are enzymes capable to break down ester bonds present in the structure of phthalates. In this research, growth, protein content and esterases activity by biochemical tests and polyacrylamide gel electrophoresis were characterized for F. culmorum grown in DEHP-supplemented (100 and 1500 mg/L) media as the only carbon source in liquid fermentation. F. culmorum showed higher biomass production and esterase activity in medium supplemented with 1500 mg of DEHP/L. Zymography revealed that bands with esterase activity were observed after 24 h and 48 h in media supplemented with 1500 and 100 mg of DEHP/L, respectively. It was shown that DEHP is an inducer of esterases and that this compound was used as carbon and energy sources by F. culmorum. This fungus can secrete specific esterase to breakdown high concentrations of DEHP, being a promising organism for bioremediation of DEHP-polluted environments in both aquatic and terrestrial ecosystems.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Desempeño productivo, características de la canal y calidad de la carne de cerdos alimentados con dietas base sorgo-pasta de soya adicionadas con metionina de cromo en diferentes etapas de desarrollo 不同发育阶段饲喂添加蛋氨酸铬的高粱豆粕型日粮的猪的生产性能、胴体特征和肉质

Mexican Journal of Biotechnology Pub Date : 2018-10-01 DOI: 10.29267/mxjb.2018.3.4.1

J. M. González-Alvarado, Tania Gómez-Hernández, Nohemi Hernández-Camacho, Edmundo W. Sánchez-Márquez, Antonio J. Soto-Sánchez, Marco Antonio Cruz-Portillo, J. Aragón-Hernández, Adelfo Vite-Aranda, J. Yáñez-Hernández

{"title":"Desempeño productivo, características de la canal y calidad de la carne de cerdos alimentados con dietas base sorgo-pasta de soya adicionadas con metionina de cromo en diferentes etapas de desarrollo","authors":"J. M. González-Alvarado, Tania Gómez-Hernández, Nohemi Hernández-Camacho, Edmundo W. Sánchez-Márquez, Antonio J. Soto-Sánchez, Marco Antonio Cruz-Portillo, J. Aragón-Hernández, Adelfo Vite-Aranda, J. Yáñez-Hernández","doi":"10.29267/mxjb.2018.3.4.1","DOIUrl":"https://doi.org/10.29267/mxjb.2018.3.4.1","url":null,"abstract":"Se evaluó el efecto de la suplementación con metionina de cromo (Met-Cr) sobre el desempeño productivo y características de la canal en cerdos en finalización. Se evaluaron 3 tratamientos; 1) testigo en donde los cerdos fueron alimentados con dietas de finalización de 60 a 100 kg; 2) testigo + 0.8 mg de Cr/kg de alimento desde 60 hasta 100 kg; y 3) testigo + 0.8 mg de Cr/kg de alimento desde 80 hasta 100 kg. Los cerdos suplementados con MetCr consumieron menos alimento (P≤0.05), crecieron más rápido (P≤0.05) y fueron más pesados (P≤0.05) y más eficientes (P≤0.05) que los cerdos testigo, pero el efecto benéfico fue mayor cuando la suplementación inició a menor peso. La adición de Met-Cr incrementó el área del músculo Longissimus dorsi (P≤0.01), el rendimiento de canal (P≤0.01) y redujo el espesor de grasa dorsal (P≤0.01). Además, la carne de cerdos suplementados presentó valores de pH y luminosidad (L*) más bajos (P≤0.01) que de cerdos testigo. Incluso, el consumo de Met-Cr redujo las pérdidas a la cocción (P≤0.01) y la fuerza de corte (P≤0.01), pero el efecto sobre la suavidad fue mayor al suplementar a menor peso. En conclusión, la suplementación de Met-Cr en cerdos mejora el desempeño productivo, las características de la canal y algunos indicadores de calidad de la carne, pero la etapa en que inicia la suplementación con Cr puede afectar la respuesta animal.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44289209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diseño y aplicación in vitro de consorcios microbianos degradadores de hidrocarburos 碳氢化合物降解微生物组合的设计与体外应用

Mexican Journal of Biotechnology Pub Date : 2018-10-01 DOI: 10.29267/MXJB.2018.3.4.33

María de los Ángeles Romero-Bañuelos, Adán Topiltzin Morales-Vargas, Varinia López Ramírez

引用次数: 0

Biological links between nanoparticle biosynthesis and stress responses in bacteria 纳米颗粒生物合成与细菌应激反应之间的生物学联系

Mexican Journal of Biotechnology Pub Date : 2018-10-01 DOI: 10.29267/MXJB.2018.3.4.44

Angela Chen, B. Keitz, Lydia M. Contreras

{"title":"Biological links between nanoparticle biosynthesis and stress responses in bacteria","authors":"Angela Chen, B. Keitz, Lydia M. Contreras","doi":"10.29267/MXJB.2018.3.4.44","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.4.44","url":null,"abstract":"There is rising interest in nanoparticle biosynthesis using bacteria due to the potential for applications in bioremediation, catalysis, or as antimicrobials. However, biosynthesis remains limited by the inability to control nanoparticle morphology and size due to the lack of knowledge regarding explicit molecular mechanisms. Due to their importance in nanoparticle biosynthesis and as antimicrobials, we focus our discussion on silver, gold, and copper nanoparticles. We discuss recent efforts to elucidate reduction mechanisms that have identified generic enzymes and metal resistance genes as strong candidates to facilitate nanoparticle biosynthesis. Although it is known that these enzymes and genes play significant roles in maintaining bacterial homeostasis, there are few reports discussing this topic. Thus, we discuss examples of how metal resistance genes are conserved across bacteria and have been shown to be important for both nanoparticle biosynthesis and processes such as virulence or oxidative stress responses. Overall, this review highlights biological connections between nanoparticle biosynthesis and stress responses by examining\u0000the role of reductases and metal resistance genes in both processes. This understanding provides a greater role for nanoparticle biosynthesis in bacteria and could enable a systems biology level of control over nanoparticle biosynthesis.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46208474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic effects of interferons in human viral infections 干扰素对人类病毒感染的治疗作用

Mexican Journal of Biotechnology Pub Date : 2018-10-01 DOI: 10.29267/MXJB.2018.3.4.19

Hebert Jair Barrales-Cureño, C. Reyes-Reyes, Maximino Díaz-Bautista, A. D. Jesús, Salvador Chávez-Salinas, L. G. López-Valdez

引用次数: 0

Prevalencia de genes de virulencia de Escherichia coli en aguas superficiales del Río Bravo en la ciudad de Reynosa, Tamaulipas 塔毛利帕斯州雷诺萨市里约热内卢Bravo地表水中大肠杆菌毒力基因的流行情况

Mexican Journal of Biotechnology Pub Date : 2018-07-01 DOI: 10.29267/MXJB.2018.3.3.87

Rocío Requena-Castro, María Guadalupe Aguilera-Arreola, A. V. Martínez-Vázquez, Virgilio Bocanegra-García

引用次数: 1

Biological treatment of coal combustion wastes by Acidithiobacillus thiooxidans DSM 26636 酸性硫氧化硫杆菌DSM 26636对燃煤废弃物的生物处理

Mexican Journal of Biotechnology Pub Date : 2018-07-01 DOI: 10.29267/MXJB.2018.3.3.54

N. Rojas-Avelizapa, Itzayana V. Hipólito-Júarez, M. Gómez-Ramírez

{"title":"Biological treatment of coal combustion wastes by Acidithiobacillus thiooxidans DSM 26636","authors":"N. Rojas-Avelizapa, Itzayana V. Hipólito-Júarez, M. Gómez-Ramírez","doi":"10.29267/MXJB.2018.3.3.54","DOIUrl":"https://doi.org/10.29267/MXJB.2018.3.3.54","url":null,"abstract":"The high levels of toxicity generated by the heavy metal content in industrial wastes has generated environmental and health concerns. One of the strategies to reduce the metallic load is the use of sulfur-oxidizing bacteria, due to its ability to produce sulfuric acid involved in the metal leaching. The aim of this research was to evaluate the growth of Acidithioobacillus thiooxidans DSM 26636 and its ability to leach metals from slags and ashes from coal combustion wastes. Microbial growth was monitoring by sulfate and\u0000sulfuric acid production. The metal content in slags and fly ashes was determined by ICPOES. The experiments were carried out during 21 days at 30°C, 150 rpm in 125 mL Erlenmeyer flasks containing 30 mL of Starkey media added with 1% (w/v) of elemental sulfur and 1% (w/v) of slags or ashes. Results showed that Acidithioobacillus thiooxidans was able to leach V, Fe, Mg, Al, Si and Ni from slags. For fly ashes, metal leaching was Al, Ni, Sn, Mg, Zn and Si. Summarizing, Acidithioobacillus thiooxidans could be used for the leaching of different metals contained in wastes from carbon combustion plant.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43979548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Biosorption of mercury (II) from aqueous solution onto biomass of Aspergillus niger 水溶液中汞（II）在黑曲霉生物量上的吸附

Mexican Journal of Biotechnology Pub Date : 2018-07-01 DOI: 10.29267/MXJB.2018.3.3.15

I. A. Rodríguez, Nancy Cecilia Pacheco-Castillo, J. Cárdenas-González, M. Zárate, V. Martínez-Juárez, A. Rodríguez-Pérez

引用次数: 0