Mexican Journal of Biotechnology最新文献

Mycelial growth and production of laccase and peroxidases by Pleurotus ostreatus and Agrocybe aegerita in liquid fermentation 在液体发酵过程中的菌丝生长以及黑木耳和姬松茸产生漆酶和过氧化物酶的情况

Mexican Journal of Biotechnology Pub Date : 2024-07-01 DOI: 10.29267/mxjb.2024.9.3.36

Angel González-Márquez, Carmen Sánchez

引用次数: 0

Bacterial community structure associated with roots of Pinus montezumae Lamb. inoculated with edible ectomycorrhizal fungi and mycorrhizal helper bacteria 接种了可食用的外生菌根真菌和菌根辅助菌的欧洲赤松（Pinus montezumae Lamb.）根部的细菌群落结构

Mexican Journal of Biotechnology Pub Date : 2024-07-01 DOI: 10.29267/mxjb.2024.9.3.1

J. D. Álvarez-Solís, María del Rosario Cardoso-Villanueva, B. Xoconostle‐Cazares, M. E. Sánchez-García, José Luis Barragán-Soriano, J. Pérez‐Moreno

{"title":"Bacterial community structure associated with roots of Pinus montezumae Lamb. inoculated with edible ectomycorrhizal fungi and mycorrhizal helper bacteria","authors":"J. D. Álvarez-Solís, María del Rosario Cardoso-Villanueva, B. Xoconostle‐Cazares, M. E. Sánchez-García, José Luis Barragán-Soriano, J. Pérez‐Moreno","doi":"10.29267/mxjb.2024.9.3.1","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.3.1","url":null,"abstract":"Tree roots are associated with a high diversity of microorganisms that play key roles in soil fertility and plant nutrition and health. Here we examine the composition and diversity of bacterial community in ectomycorrhizal root (ECR) and their ectomycorrhizosphere (EMR) of the Neotropical tree Pinus montezumae inoculated with Laccaria laccata and Hebeloma mesophaeum, applied alone or co-inoculated with Azospirillum brasilense. For this, we used a 16S rRNA gene metabarcoding-sequencing approach in samples of ECR and EMR that were collected nine-months after inoculation in greenhouse. A total of 943 Operational Taxonomic Units (OTU) were differentiated and grouped in 32 bacterial phyla. The most abundant phyla were Proteobacteria (67%) and Actinobacteria (20.7%). Bacterial communities were structured in ECR and EMR (Anosim, R=0.77, P≤0.005), and were more diverse in EMR than ECR (Shannon-Weaver of 4.2 and 3.6, and Simpson inverse of 33.7 and 16.7, respectively). The abundance of Pseudomonas, Actinoplanes and Salinibacterium were promoted with L. laccata, while with H. mesophaeum an OTU from Burkholderiaceae was prominent. Our results showed that the ectomycorrhizal fungi strongly shaped the composition of the bacterial communities associated with roots of P. montezumae.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"52 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141696220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth and invertase production by Neurospora crassa in submerged and solid-state cultures 沉水和固态培养条件下十字花科黑孢子菌的生长和转化酶的产生

Mexican Journal of Biotechnology Pub Date : 2024-07-01 DOI: 10.29267/mxjb.2024.9.3.20

Christian Jesús Mora-Pérez, Montserrat Citlali Sánchez-Ruiz, Daniel Salgado-Bautista, U. Carrasco-Navarro, E. Favela‐Torres

{"title":"Growth and invertase production by Neurospora crassa in submerged and solid-state cultures","authors":"Christian Jesús Mora-Pérez, Montserrat Citlali Sánchez-Ruiz, Daniel Salgado-Bautista, U. Carrasco-Navarro, E. Favela‐Torres","doi":"10.29267/mxjb.2024.9.3.20","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.3.20","url":null,"abstract":"Filamentous fungi are widely used in industry to produce enzymes, organic acids, and secondary metabolites. Neurospora crassa has gained attention due to its flexibility for easy genetic manipulation, fast growth, and non-pathogenic characteristics. This study evaluated the growth of N. crassa and invertase production in submerged culture (SC) and solid-state culture (SSC) using pine sawdust (SSC-PS) and polyurethane foam (SSC-PUF) as supports. Modified Vogel's medium with initial sucrose concentrations of 1.5%, 3%, and 5% was used. The specific and maximum CO2 production rates were higher in SSC than SC, particularly with SSC-PS. Protein and invertase production were higher in SSC, with SSC-PS demonstrating the highest sucrose concentration as the inducer and carbon source. The water-holding capacity (WHC) of SSC-PUF was approximately 25 times higher than that of SSC-PS, facilitating a more productive process. SSC-PUF enables higher biomass growth, protein, and invertase production than SC. Moreover, using inert supports, such as PUF, allows the correct substrates and product assessment without interferences due to the chemical composition and heterogeneity of conventional agro-industrial by-products, such as PS.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"37 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidases production by Trametes versicolor grown on green waste and on polyurethane foam in solid-state fermentation: A comparative study 固态发酵法在绿色废物和聚氨酯泡沫上生长的多色曲霉产生氧化酶：比较研究

Mexican Journal of Biotechnology Pub Date : 2024-04-20 DOI: 10.29267/mxjb.2024.9.2.51

Edgardo Ocaña-Romo, C. O. Rodríguez-Nava, Carmen Sánchez

{"title":"Oxidases production by Trametes versicolor grown on green waste and on polyurethane foam in solid-state fermentation: A comparative study","authors":"Edgardo Ocaña-Romo, C. O. Rodríguez-Nava, Carmen Sánchez","doi":"10.29267/mxjb.2024.9.2.51","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.2.51","url":null,"abstract":"Green waste (GW) is generated by the maintenance of public or private green spaces. It is necessary to find ecological alternatives for GW utilization, aiming to avoid accumulation of this material at the environment. In this research, the production of laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and unspecific peroxygenase (UnP) produced by Trametes versicolor grown on GW as a substrate and on polyurethane foam (PUF) as an inert support in solid state fermentation was evaluated. T. versicolor showed higher values of Lac, MnP, UnP and LiP activities (34, 943, 1023 and 766 U/gS, respectively) when grown on GW than when grown on PUF (10.9, 588, 559 and 229 U/gS, respectively). These results suggest that T. versicolor produced Lac inducible and constitutively, while LiP, MnP and UnP were induced by GW at the beginning of fungal growth, however, these enzymes were constitutive and inducible during the rest of the fermentation. The production of oxidases and peroxidases was induced and increased by GW. It is suggested that LiP is involved (as a constitutive enzyme) at the beginning of the exponential phase, while MnP and UnP participate in fungal growth at the end of fermentation. To our knowledge, this is the first detailed study on the main lignincellulose-degrading fungal enzymes involved in GW degradation by fungi. In particular, the relevance of UnP was showed as peroxidase involved in lignocellulosic substrates biodegradation.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140681660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of a plant growth-promoting rhizobacteria consortium to promote growth in lettuce (Lactuca sativa L.) and chili pepper (Capsicum annuum L.) 植物生长促进根瘤菌群对促进莴苣（Lactuca sativa L.）和辣椒（Capsicum annuum L.）生长的影响

Mexican Journal of Biotechnology Pub Date : 2024-04-19 DOI: 10.29267/mxjb.2024.9.2.19

Franco Ignacio Bugueño-Guerrero, Orlando Catalán-Barrera, M. P. Carballo-Sánchez

引用次数: 0

Effect of different concentrations of benzylaminopurine for the in vitro propagation of Vanilla tahitensis (J. W. Moore) 不同浓度的苄基氨基嘌呤对香草（J. W. Moore）体外繁殖的影响

Mexican Journal of Biotechnology Pub Date : 2024-04-19 DOI: 10.29267/mxjb.2024.9.2.34

Laura Lucia Parismoreno-Rivas, Bryan Stalin Barcia-Jalca, R. Medina-Litardo, I. Pérez-Almeida

{"title":"Effect of different concentrations of benzylaminopurine for the in vitro propagation of Vanilla tahitensis (J. W. Moore)","authors":"Laura Lucia Parismoreno-Rivas, Bryan Stalin Barcia-Jalca, R. Medina-Litardo, I. Pérez-Almeida","doi":"10.29267/mxjb.2024.9.2.34","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.2.34","url":null,"abstract":"The establishment of an efficient in vitro propagation protocol is still ongoing for Vanilla tahitensis (J. W. Moore), an underdeveloped but economically attractive crop in Ecuador. The objective of the research was to evaluate the application of different concentrations of cytokine benzylaminopurine (BAP) in its in vitro culture. The effect of six concentrations of BAP was studied (0.50, 1.00, 1.50, 1.75, 2.00 and 2.50 mg/L), and a control treatment, with five repetitions, using a completely randomized design. The 2.0 mg/L concentration of BAP allowed the best micropropagation of the vanilla producing 5 and 6 shoots at 60 and 90 d after sowing. The greatest length of roots was achieved with 1.75 mg/L (7 cm). Regarding plant height and leaf number, the 2.0 mg/L concentration showed the highest plant height with 18 and 6 cm at 90 and 60 d after sowing, and the highest number of leaves with 7 and 5 leaves, respectively. This research reports a successful in vitro culture protocol for this species, to promote the development of this crop in Ecuador, due to its outstanding possibilities for commerce at the international market.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":" 346","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140682386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mutation of the MIR867 gene causes seed abortion and affects fruit size in the model plant Arabidopsis thaliana MIR867 基因突变会导致模式植物拟南芥种子流产并影响果实大小

Mexican Journal of Biotechnology Pub Date : 2024-04-01 DOI: 10.29267/mxjb.2024.9.2.1

Yemitzel Camarero-Arellano, J. Badillo-Corona, Andrea Tovar-Aguilar, N. Durán-Figueroa

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Proceedings of the X International Congress on Biotechnology SOCIBI, IV Meeting of Students and Alumni of the CICB-UATx 第十届国际生物技术大会（SOCIBI）论文集，第四届 CICB-UATx 学生和校友会议

Mexican Journal of Biotechnology Pub Date : 2024-01-15 DOI: 10.29267/mxjb.2024.9.1.33

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Bioactive compounds in Laelia speciosa (Orchidaceae) seedlings grown in temporary immersion bioreactor 在临时浸泡生物反应器中培育的兰花（兰科）幼苗中的生物活性化合物

Mexican Journal of Biotechnology Pub Date : 2024-01-05 DOI: 10.29267/mxjb.2024.9.1.19

Ana Laura López-Escamilla, Viridiana Badillo-Huerta, Patricia Rodríguez-Cuamatzi, J. García-Dávila, Lilia Sánchez-Minutti

引用次数: 0

Alternative for increasing enzymatic activity and sugar release in organic solid waste during aerobic degradation process 在有氧降解过程中提高有机固体废物中酶的活性和糖分释放的替代方法

Mexican Journal of Biotechnology Pub Date : 2024-01-05 DOI: 10.29267/mxjb.2024.9.1.1

Castillo-Minjarez J.M., Vargas-León E.A., Quezada M., Martínez-Valdez F. J.

{"title":"Alternative for increasing enzymatic activity and sugar release in organic solid waste during aerobic degradation process","authors":"Castillo-Minjarez J.M., Vargas-León E.A., Quezada M., Martínez-Valdez F. J.","doi":"10.29267/mxjb.2024.9.1.1","DOIUrl":"https://doi.org/10.29267/mxjb.2024.9.1.1","url":null,"abstract":"The impact of inoculum (mature compost) was assessed in an aerobic system for the rapid degradation of organic solid waste, with a focus on the release of reducing sugars. Isothermal (35 and 55 °C) and non-isothermal (35-55 °C) treatments were conducted. Key indicators, including the rate of oxygen consumption (O2) and carbon dioxide production (CO2), were measured to assess microbial activity. Additionally, the concentration of reducing sugars, pH, enzymatic activity, and the presence of pathogens were evaluated. Treatments with inoculum at 35 °C and without inoculum at 55 °C exhibited an increase in oxygen consumption and CO2 production, indicating heightened microbial activity and, consequently, greater mineralization of organic matter. The treatment without inoculum at 35-55 °C presented the highest concentration of reducing sugars (160 mg/g dry weight), suggesting its potential as a pretreatment for bioconversion processes aimed at generating value-added products. At a temperature of 55 °C, there was a reduction of 84.4, 87.28, and 67.33 % in the concentrations of fecal coliforms, Escherichia coli, and parasites, respectively. This result suggests that elevated temperature contributes to the effectiveness of pathogen elimination.","PeriodicalId":36479,"journal":{"name":"Mexican Journal of Biotechnology","volume":"40 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0