Biosafety and Health最新文献_第8页

Viral rebound and safety of nirmatrelvir/ritonavir for lung-transplant recipients infected with SARS-CoV-2 尼马特雷韦/利托那韦治疗严重急性呼吸系统综合征冠状病毒2型肺移植受者的病毒反弹和安全性

Biosafety and Health Pub Date : 2023-10-01 DOI: 10.1016/j.bsheal.2023.08.004

Hui Li , Li Zhao , Ke Huang , Xiaoxing Wang , Fei Zhou , Yiming Feng , Liang Ma , Bin Cao , Wenhui Chen

{"title":"Viral rebound and safety of nirmatrelvir/ritonavir for lung-transplant recipients infected with SARS-CoV-2","authors":"Hui Li , Li Zhao , Ke Huang , Xiaoxing Wang , Fei Zhou , Yiming Feng , Liang Ma , Bin Cao , Wenhui Chen","doi":"10.1016/j.bsheal.2023.08.004","DOIUrl":"10.1016/j.bsheal.2023.08.004","url":null,"abstract":"<div><p>Data on the viral rebound and safety of nirmatrelvir/ritonavir in lung transplant (LTx) recipients are limited. The study prospectively followed four LTx recipients. Clinical characteristics, viral RNA dynamic in throat swabs, and tacrolimus blood concentration were monitored regularly. All four LTx recipients, aged 35–74 years, were not vaccinated against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). They got coronavirus disease 2019 (COVID-19) after more than one week of admission during the era of Omicron. All cases received nirmatrelvir/ritonavir (NM/r) within two days of infection, and the relative viral RNA copies dropped quickly. Viral load rebound was observed in all four cases after discontinuation of the first five days of NM/r treatment. Three of them received another 5-days antiviral therapy with NM/r. The duration of positive viral PCR testing was 25-28 days. None of them progressed into severe or critical COVID-19. Tacrolimus was stopped 12 h before NM/r and held during the 5-day course of antiviral therapy. Blood concentration of tacrolimus were maintained at a baseline level during these five days. Tacrolimus was re‐initiated at its baseline daily dose 3-4 days after NM/r therapy. However, during the second round of antiviral therapy with NM/r, the concentration of tacrolimus fluctuated wildly. In conclusion, the 5-day course of NM/r treatment was not sufficient for LTx recipients and the viral rebound was common. More data are needed to clarify whether LTx recipients with SARS-CoV-2 viral rebound could benefit from additional treatment with NM/r.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 266-271"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43864855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incorporation of Escherichia coli heat-labile enterotoxin B subunit into rabies virus particles enhances its immunogenicity in mice and dogs 将大肠杆菌热不稳定肠毒素B亚基掺入狂犬病毒颗粒中，增强了狂犬病毒在小鼠和犬体内的免疫原性

Biosafety and Health Pub Date : 2023-10-01 DOI: 10.1016/j.bsheal.2023.05.005

Zhiyuan Gong , Hailun Li , Meichen Qian , Yujie Bai , Hongli Jin , Jingxuan Sun , Mengyao Zhang , Cuicui Jiao , Pei Huang , Yuanyuan Li , Haili Zhang , Hualei Wang

{"title":"Incorporation of Escherichia coli heat-labile enterotoxin B subunit into rabies virus particles enhances its immunogenicity in mice and dogs","authors":"Zhiyuan Gong , Hailun Li , Meichen Qian , Yujie Bai , Hongli Jin , Jingxuan Sun , Mengyao Zhang , Cuicui Jiao , Pei Huang , Yuanyuan Li , Haili Zhang , Hualei Wang","doi":"10.1016/j.bsheal.2023.05.005","DOIUrl":"10.1016/j.bsheal.2023.05.005","url":null,"abstract":"<div><p>Although inactivated vaccines against rabies have the advantage of high safety, effective protection against rabies virus (RABV) infection often requires multiple, high-dose immunization. Incorporating a molecular adjuvant into the viral particles has been found to be a useful strategy to promote the immune effectiveness of inactivated vaccines. In this study, we constructed a recombinant virus, rCVS11-LTB, which chimerically expresses a molecular adjuvant heat-labile enterotoxin B subunit (LTB) protein on the surface of the RABV particles. Immunogenicity <em>in vivo</em> was found to be promoted by rCVS11-LTB through the activation of dendritic cells (DCs). Our results demonstrated that inactivated rCVS11-LTB was able to induce higher levels of virus-neutralizing antibodies (VNAs) in both mice and dogs than the parent virus rCVS11, to enhance the cellular immune response and T cell immune memory in mice, and was also able to provide 100% protection in mice from lethal doses of rabies virus, indicating its potential as a safe and effective inactivated rabies vaccine candidate.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 308-319"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48445393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The recombinant truncated envelope protein of West Nile virus adjuvanted with Alum/CpG induces potent humoral and T cell immunity in mice Alum/CpG修饰的西尼罗河病毒截短包膜蛋白诱导小鼠产生强大的体液免疫和T细胞免疫

Biosafety and Health Pub Date : 2023-10-01 DOI: 10.1016/j.bsheal.2023.06.003

Yongping Du , Yao Deng , Ying Zhan , Ren Yang , Jiao Ren , Wen Wang , Baoying Huang , Wenjie Tan

{"title":"The recombinant truncated envelope protein of West Nile virus adjuvanted with Alum/CpG induces potent humoral and T cell immunity in mice","authors":"Yongping Du , Yao Deng , Ying Zhan , Ren Yang , Jiao Ren , Wen Wang , Baoying Huang , Wenjie Tan","doi":"10.1016/j.bsheal.2023.06.003","DOIUrl":"https://doi.org/10.1016/j.bsheal.2023.06.003","url":null,"abstract":"<div><p>West Nile virus (WNV) is a mosquito-transmitted flavivirus distributed globally for decades and can cause disease in humans and animals. So far, no WNV vaccine has been licensed for human use. Therefore, the development of novel candidate vaccines and the improvement of vaccination strategies is imperative. As the WNV envelope (E) glycoprotein plays an important role in mediating viral binding to cellular receptors and virus-cell membrane fusion, it is a critical target for the host humoral response. Here, we prepared a recombinant truncated envelope protein of WNV (rWNV-80E) and developed a WNV subunit vaccine formulation with a combination of aluminum hydroxide (alum) and a synthetic oligonucleotide CpG as adjuvants. C57BL/6 mice were immunized twice intramuscularly at 28-day intervals with 5 µg purified rWNV-80E adjuvanted with Alum/CpG. WNV E-specific IgG was detected by enzyme-linked immunosorbent assay and neutralizing antibodies (nAbs) was detected using single-round infectious particles of WNV. Furthermore, T cell immunity was detected by enzyme-linked immunospot assay and intracellular cytokine staining assay. Notably, rWNV-80E was highly immunogenic and elicited potent humoral and cell immunity, as evidenced by significant levels of IFN-γ and TNF-α secretion in the T cells of mice. In summary, the Alum/CpG-adjuvanted rWNV-80E subunit vaccine elicited potent and balanced B- and T-cell immunity in mice, and therefore it is a promising candidate vaccine that warrants further investigation for use in human or veterinary applications.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 5","pages":"Pages 300-307"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67739761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of innate immunity in SARS-CoV-2 infection 先天免疫在严重急性呼吸系统综合征冠状病毒2型感染中的作用

Biosafety and Health Pub Date : 2023-10-01 DOI: 10.1016/j.bsheal.2023.08.005

Zihao Wang , Fang Cheng , Yuxiu Xu , Xin Li , Songdong Meng

引用次数: 0

Revealing the roles of TLR7, a nucleic acid sensor for COVID-19 in pan-cancer 揭示新冠病毒核酸传感器TLR7在泛癌中的作用

Biosafety and Health Pub Date : 2023-08-01 DOI: 10.1016/j.bsheal.2023.05.004

Zhijian Huang , Yaoxin Gao , Yuanyuan Han , Jingwen Yang , Can Yang , Shixiong Li , Decong Zhou , Qiuyan Huang , Jialiang Yang

{"title":"Revealing the roles of TLR7, a nucleic acid sensor for COVID-19 in pan-cancer","authors":"Zhijian Huang , Yaoxin Gao , Yuanyuan Han , Jingwen Yang , Can Yang , Shixiong Li , Decong Zhou , Qiuyan Huang , Jialiang Yang","doi":"10.1016/j.bsheal.2023.05.004","DOIUrl":"10.1016/j.bsheal.2023.05.004","url":null,"abstract":"<div><p>Recent studies suggested that cancer was a risk factor for coronavirus disease 2019 (COVID-19). Toll-like receptor 7 (TLR7), a severe acute respiratory syndrome 2 (SARS-CoV-2) virus's nucleic acid sensor, was discovered to be aberrantly expressed in many types of cancers. However, its expression pattern across cancers and association with COVID-19 has not been systematically studied. In this study, we proposed a computational framework to comprehensively study the roles of TLR7 in COVID-19 and pan-cancers at genetic, gene expression, protein, epigenetic, and single-cell levels. We found TLR7 mRNA expression was significantly up-regulated in 6 cancer types and down-regulated in 6 cancer types, further validated in the HPA database at the protein level. The genes significantly co-expressed with TLR7 were mainly enriched in the toll-like receptor signaling pathway, endolysosome, and signaling pattern recognition receptor activity. In addition, the abnormal TLR7 expression was associated with Mismatch repair (MMR), microsatellite instability (MSI), tumor mutational burden (TMB) in various cancers. Mined by the ESTIMATE algorithm, the expression of TLR7 was also closely linked to various immune infiltration patterns in pan-cancer, and TLR7 was mainly enriched in macrophages, as revealed by single-cell RNA sequencing. Finally, TLR7 expressions were very sensitive to a few targeted drugs, such as Alectinib and Imiquimod. In conclusion, TLR7 might be essential in the pathogenesis of COVID-19 and cancers.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 211-226"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9695706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Early warning of emerging infectious diseases based on multimodal data 基于多模式数据的新发传染病早期预警

Biosafety and Health Pub Date : 2023-08-01 DOI: 10.1016/j.bsheal.2023.05.006

Haotian Ren , Yunchao Ling , Ruifang Cao , Zhen Wang , Yixue Li , Tao Huang

{"title":"Early warning of emerging infectious diseases based on multimodal data","authors":"Haotian Ren , Yunchao Ling , Ruifang Cao , Zhen Wang , Yixue Li , Tao Huang","doi":"10.1016/j.bsheal.2023.05.006","DOIUrl":"10.1016/j.bsheal.2023.05.006","url":null,"abstract":"<div><p>The coronavirus disease 2019 (COVID-19) pandemic has dramatically increased the awareness of emerging infectious diseases. The advancement of multiomics analysis technology has resulted in the development of several databases containing virus information. Several scientists have integrated existing data on viruses to construct phylogenetic trees and predict virus mutation and transmission in different ways, providing prospective technical support for epidemic prevention and control. This review summarized the databases of known emerging infectious viruses and techniques focusing on virus variant forecasting and early warning. It focuses on the multi-dimensional information integration and database construction of emerging infectious viruses, virus mutation spectrum construction and variant forecast model, analysis of the affinity between mutation antigen and the receptor, propagation model of virus dynamic evolution, and monitoring and early warning for variants. As people have suffered from COVID-19 and repeated flu outbreaks, we focused on the research results of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses. This review comprehensively viewed the latest virus research and provided a reference for future virus prevention and control research.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 193-203"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9687588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Simultaneous detection of enterovirus-D68 and vaccine-related poliovirus 3 in the stool samples of a 5-month hospitalized child with acute respiratory disease: A case report 急性呼吸道疾病住院5个月儿童粪便样本中同时检测肠道病毒- d68和疫苗相关脊髓灰质炎病毒3:1例报告

Biosafety and Health Pub Date : 2023-08-01 DOI: 10.1016/j.bsheal.2023.07.002

Wenzhe Su , Qing Zeng , Jinmei Geng , Jingwen Liu , Huaping Xie , Kuibiao Li , Pengzhe Qin , Chaojun Xie , Biao Di

{"title":"Simultaneous detection of enterovirus-D68 and vaccine-related poliovirus 3 in the stool samples of a 5-month hospitalized child with acute respiratory disease: A case report","authors":"Wenzhe Su , Qing Zeng , Jinmei Geng , Jingwen Liu , Huaping Xie , Kuibiao Li , Pengzhe Qin , Chaojun Xie , Biao Di","doi":"10.1016/j.bsheal.2023.07.002","DOIUrl":"10.1016/j.bsheal.2023.07.002","url":null,"abstract":"<div><p>Human enterovirus (EV) infections can lead to various manifestations, with variable correlations between genotypes and symptoms. Human enterovirus D68 (EV-D68) was considered to be associated with acute respiratory disease and acute flaccid myelitis. In this short report, both EV-D68 and poliovirus 3 were detected in the stool of a hospitalized 5-month child who presented with acute respiratory symptoms and who was recently vaccinated with oral polio vaccine (OPV), using a metatranscriptomic high-throughput sequencing method. The nearly full-length genome sequences with complete open reading frames of EV-D68 and poliovirus 3 were assembled. One previously-reported neurovirulence-related amino acid substitution (T860N) in the EV-D68 VP1 region was observed, but the patient showed no neurological symptoms. More attention should be paid to EV-D68, and continuous multiple syndrome-based surveillance on non-polio enterovirus is called for.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 250-253"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41381250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the distribution characteristics of enterovirus types based on environmental surveillance from 2013 to 2021 in Fujian Province, China 基于2013 - 2021年福建省环境监测的肠道病毒类型分布特征分析

Biosafety and Health Pub Date : 2023-08-01 DOI: 10.1016/j.bsheal.2023.05.003

Xiuhui Yang , Shaojian Cai , Xiaoqian Wu , Yong Zhang , Dong Li , Yahong Chen , Qianjing Chen , Shuangli Zhu , Dongmei Yan , Wenbo Xu , Hairong Zhang , Zhifei Chen , Suhan Zhang , Yong Zhou , Mengping Zhang , Ningxuan Zheng , Na You

{"title":"Analysis of the distribution characteristics of enterovirus types based on environmental surveillance from 2013 to 2021 in Fujian Province, China","authors":"Xiuhui Yang , Shaojian Cai , Xiaoqian Wu , Yong Zhang , Dong Li , Yahong Chen , Qianjing Chen , Shuangli Zhu , Dongmei Yan , Wenbo Xu , Hairong Zhang , Zhifei Chen , Suhan Zhang , Yong Zhou , Mengping Zhang , Ningxuan Zheng , Na You","doi":"10.1016/j.bsheal.2023.05.003","DOIUrl":"10.1016/j.bsheal.2023.05.003","url":null,"abstract":"<div><p>Environmental surveillance (ES) is a useful approach for monitoring circulating viruses, including polioviruses (PVs) and non-polio enteroviruses (NPEVs). In this study, the results of nine years of ES from 2013 to 2021 at six sampling sites in three cities in Fujian Province, China, were summarized. It showed that the sewage samples contained abundant viruses, but the positive rate was affected by different sampling sites. From the 520 samples, 431 PVs, 1,713 NPEVs, and 281 human adenoviruses (HAdVs) were isolated. PV isolates had been markedly affected following the adjustment of the immunization strategy. All but one PV isolate were Sabin-like strains without wild PVs. One isolate was vaccine-derived PV type 3 with 10 variation points in the <em>VP1</em> region. After May 2016, PV type 2 was no longer detected, and PV type 3 became a superior serotype. Of 1,713 NPEVs, 24 serotypes were identified, including echovirus11 (E11), E6, coxsackievirus B3 (CVB3), CVB5, E7, and E3 were the predominant serotypes (37.65%, 20.96%, 11.50%, 8.87%, 8.23%, and 7.06%, respectively). The temporal dynamic of the six common serotypes was inconsistent. E3 was frequently isolated, but the number of isolates was low, with no obvious peaks. E6, E7, and CVB3 exhibited periodic changes with a high peak every three to four years, and E11 only had one high peak lasting four years. Summer-fall peaks of the echoviruses and spring-winter peaks of CVB were observed in the monthly distribution of virus isolation. The infectious isolates of various serotypes of different species identified from the sewage samples showed that ES is an essential part of pathogen surveillance.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 240-249"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44723474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Forecast the potential SARS-CoV-2 variants in the future and predict their biological properties and social impacts from bioinformatics and public health perspectives 预测未来潜在的严重急性呼吸系统综合征冠状病毒2型变异株，并从生物信息学和公共卫生角度预测其生物学特性和社会影响

Biosafety and Health Pub Date : 2023-08-01 DOI: 10.1016/j.bsheal.2023.07.005

Yixue Li , Yuming Guo , Tao Huang

引用次数: 0

On the collection and integration of SARS-CoV-2 genome data 关于严重急性呼吸系统综合征冠状病毒2型基因组数据的收集和整合

Biosafety and Health Pub Date : 2023-08-01 DOI: 10.1016/j.bsheal.2023.07.004

Lina Ma , Wei Zhao , Tianhao Huang , Enhui Jin , Gangao Wu , Wenming Zhao , Yiming Bao

{"title":"On the collection and integration of SARS-CoV-2 genome data","authors":"Lina Ma , Wei Zhao , Tianhao Huang , Enhui Jin , Gangao Wu , Wenming Zhao , Yiming Bao","doi":"10.1016/j.bsheal.2023.07.004","DOIUrl":"10.1016/j.bsheal.2023.07.004","url":null,"abstract":"<div><p>Genome data of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for virus diagnosis, vaccine development, and variant surveillance. To archive and integrate worldwide SARS-CoV-2 genome data, a series of resources have been constructed, serving as a fundamental infrastructure for SARS-CoV-2 research, pandemic prevention and control, and coronavirus disease 2019 (COVID-19) therapy. Here we present an overview of extant SARS-CoV-2 resources that are devoted to genome data deposition and integration. We review deposition resources in data accessibility, metadata standardization, data curation and annotation; review integrative resources in data source, de-redundancy processing, data curation and quality assessment, and variant annotation. Moreover, we address issues that impede SARS-CoV-2 genome data integration, including low-complexity, inconsistency and absence of isolate name, sequence inconsistency, asynchronous update of genome data, and mismatched metadata. We finally provide insights into data standardization consensus and data submission guidelines, to promote SARS-CoV-2 genome data sharing and integration.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 4","pages":"Pages 204-210"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45326889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2