Biosafety and Health最新文献_第9页

Pathogenesis of enterovirus infection in central nervous system. 肠病毒感染中枢神经系统的发病机制

IF 3.5

Biosafety and Health Pub Date : 2023-06-14 eCollection Date: 2023-08-01 DOI: 10.1016/j.bsheal.2023.06.001

Congcong Wang, Jichen Li, Ying Liu, Qiang Sun, Zhijun Liu

{"title":"Pathogenesis of enterovirus infection in central nervous system.","authors":"Congcong Wang, Jichen Li, Ying Liu, Qiang Sun, Zhijun Liu","doi":"10.1016/j.bsheal.2023.06.001","DOIUrl":"10.1016/j.bsheal.2023.06.001","url":null,"abstract":"<p><p>Enteroviruses (EVs) are classified into 15 species according to their sequence diversity. They include four human EV (A, B, C, and D) and three rhinoviruses (A, B, and C), and cause diseases in millions of people worldwide. Generally, individuals with enteroviral infections have mild clinical symptoms, including respiratory illness, vomiting, diarrhea, dizziness, and fever. More importantly, some members of the human EV family are neurotropic pathogens that may cause a wide range of clinical diseases, such as aseptic meningitis and encephalitis. Previously, the EV that caused the most severe neurotropic symptoms was poliovirus (PV), a member of the EV C group. Poliovirus has been eliminated in most countries through a global vaccination campaign. Non-PV EVs infect the central nervous system (CNS) and are the major EVs causing neurological diseases. These human non-PV EVs include EV A (e.g., EV-A71, CVA6, and CVA16), B (e.g., CVA9 and CVB3, CVB5, echovirus 11 [E11], E30, and E7), C (e.g., CVA24), and D (e.g., EV-D68). Here, we review the relationship between EV infection and CNS diseases and advance in the use of cellular receptors and host immune responses during viral infection.</p>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":" ","pages":"233-239"},"PeriodicalIF":3.5,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42943062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and genetic characteristics of coxsackievirus A16 associated with hand-foot-and-mouth disease in Yantai city, China in 2018–2021 烟台市2018-2021年与手足口病相关的柯萨奇病毒A16流行病学及遗传特征

Biosafety and Health Pub Date : 2023-06-01 DOI: 10.1016/j.bsheal.2023.05.001

Zhenlu Sun , Ji Wang , Xin Chi , Peihua Niu , Ruiqing Zhang , Qiao Gao , Yong Zhang , Xuejun Ma

{"title":"Epidemiology and genetic characteristics of coxsackievirus A16 associated with hand-foot-and-mouth disease in Yantai city, China in 2018–2021","authors":"Zhenlu Sun , Ji Wang , Xin Chi , Peihua Niu , Ruiqing Zhang , Qiao Gao , Yong Zhang , Xuejun Ma","doi":"10.1016/j.bsheal.2023.05.001","DOIUrl":"10.1016/j.bsheal.2023.05.001","url":null,"abstract":"<div><p>In 2008, China launched a national surveillance system for hand-foot-and-mouth disease (HFMD). Several million cases of HFMD are reported every year, coxsackievirus A16 (CVA16) was the leading cause of HFMD epidemic in Yantai city, China in recent years, but the information of epidemiology and molecular characterization of CVA16 in Yantai is limited. The aim of this study is to investigate the epidemiological characteristics and pathogenic spectrum of HFMD, and most importantly, the molecular characterization of CVA16 in Yantai from 2018 to 2021. A total of 2,000 clinical samples were collected in Yantai city from 2018 to 2021 and the enterovirus typing was performed using real-time reverse transcriptase–polymerase chain reaction (qRT-PCR). <em>VP1</em> coding regions of 41 CVA16 isolates were amplified and Sanger sequenced, and phylogenetic analysis was performed. During the study period, HFMD became prevalent from May to August each year. It peaked in June and declined in September. The incidence was highest in children aged 1 to 5 years, while more common in males than females. 1,617 out of 2,000 clinical collection of samples were tested positive for enterovirus. Among them, 614 were identified as CVA16, 45 were enterovirus A71 (EV A17), and 958 were other enterovirus serotypes. All 41 CVA16 strains belonged to the Bla and B1b genotypes. Homology analysis showed that 41 CVA16 isolates shared 83.2%–100% nucleotide and 93.7%–100% amino acid similarity among themselves. The results of this study update molecular epidemiology of CVA16 and provide a reference for HFMD prevention and control.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 3","pages":"Pages 181-186"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46190803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emvirus: An embedding-based neural framework for human-virus protein-protein interactions prediction Emvirus:一个基于嵌入的神经框架，用于预测人-病毒蛋白-蛋白相互作用

Biosafety and Health Pub Date : 2023-06-01 DOI: 10.1016/j.bsheal.2023.04.003

Pengfei Xie , Jujuan Zhuang , Geng Tian , Jialiang Yang

{"title":"Emvirus: An embedding-based neural framework for human-virus protein-protein interactions prediction","authors":"Pengfei Xie , Jujuan Zhuang , Geng Tian , Jialiang Yang","doi":"10.1016/j.bsheal.2023.04.003","DOIUrl":"10.1016/j.bsheal.2023.04.003","url":null,"abstract":"<div><p>Human-virus protein-protein interactions (PPIs) play critical roles in viral infection. For example, the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds primarily to human angiotensin-converting enzyme 2 (ACE2) protein to infect human cells. Thus, identifying and blocking these PPIs contribute to controlling and preventing viruses. However, wet-lab experiment-based identification of human-virus PPIs is usually expensive, labor-intensive, and time-consuming, which presents the need for computational methods. Many machine-learning methods have been proposed recently and achieved good results in predicting human-virus PPIs. However, most methods are based on protein sequence features and apply manually extracted features, such as statistical characteristics, phylogenetic profiles, and physicochemical properties. In this work, we present an embedding-based neural framework with convolutional neural network (CNN) and bi-directional long short-term memory unit (Bi-LSTM) architecture, named Emvirus, to predict human-virus PPIs (including human–SARS-CoV-2 PPIs). In addition, we conduct cross-viral experiments to explore the generalization ability of Emvirus. Compared to other feature extraction methods, Emvirus achieves better prediction accuracy.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 3","pages":"Pages 152-158"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9717247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Seroprevalence of human enterovirus A71 in Guangzhou, China, 2019–2021 2019-2021年广州地区人肠道病毒A71血清阳性率分析

Biosafety and Health Pub Date : 2023-06-01 DOI: 10.1016/j.bsheal.2023.05.002

Huimin Lian , Huimin Jiang , Lina Yi , Jing Sun , Huaping Xie , Ming Qiu , Limei Sun , Huifang Lin , Mingda Yang , Lin Qu , Haiyi Yang , Jing Lu , Hanri Zeng

{"title":"Seroprevalence of human enterovirus A71 in Guangzhou, China, 2019–2021","authors":"Huimin Lian , Huimin Jiang , Lina Yi , Jing Sun , Huaping Xie , Ming Qiu , Limei Sun , Huifang Lin , Mingda Yang , Lin Qu , Haiyi Yang , Jing Lu , Hanri Zeng","doi":"10.1016/j.bsheal.2023.05.002","DOIUrl":"10.1016/j.bsheal.2023.05.002","url":null,"abstract":"<div><p>Enterovirus A71 (EV-A71) is a significant hand-foot-mouth disease (HFMD) etiology. The inactivated EV-A71 vaccines were approved in China in 2016. However, the seroprevalence of EV-A71 after the vaccine application and its potential association with the EV-A71 epidemic in the population are rarely studied. In this study, we analyzed the incidence of EV-A71 infection and seroepidemiology in Guangzhou City, China. From 2019 to 2021, 167,920 clinically confirmed HFMD cases were reported in Guangzhou. In 6,868 enterovirus-positive samples, Coxsackievirus A6 and Coxsackievirus A16 were dominant genotypes, and only 3 EV-A71-positive samples were detected, highlighting the deficient epidemic activity of EV-A71. Microneutralization assay was performed on 1,000 representative serum samples. Notably, the seroprevalence and geometric mean titer (GMT) decreased significantly in 2020, and that in the < 3-year age group were increased and even higher than that in 3–5-year age group in 2019 and 2021, which was contrary to our previous surveillance result and other studies in Guangzhou. Furthermore, a moderate decline of GMT level was observed following the vaccination, but the seropositive serums were still detected for 49 months after second immunization, suggesting the long-term persistence of the immunity. Our seroepidemiology study revealed relatively higher neutralizing antibody activity in the susceptible population after the EV-A71 vaccine was adopted in 2016 in Guangzhou. It may be one of the reasons for the lower epidemic activity of EV-A71 in Guangzhou from 2019 to 2021.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 3","pages":"Pages 168-173"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48904263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk and countermeasure of laboratory-acquired infection based on pathogen transmission routes 基于病原体传播途径的实验室获得性感染风险及对策

Biosafety and Health Pub Date : 2023-06-01 DOI: 10.1016/j.bsheal.2023.04.006

Kunlan Zuo , Zongzhen Wu , Chihong Zhao , Huan Liu

引用次数: 0

Biosafety and mental health: Virus induced cognitive decline 生物安全和心理健康:病毒引起的认知能力下降

Biosafety and Health Pub Date : 2023-06-01 DOI: 10.1016/j.bsheal.2023.04.002

Chunxiao Du, Ge Li, Gencheng Han

引用次数: 0

A one-step reverse-transcription recombinase aided PCR assay for the rapid and sensitive detection of human enteroviruses 一步逆转录重组酶辅助聚合酶链式反应快速灵敏检测人肠道病毒

Biosafety and Health Pub Date : 2023-04-01 DOI: 10.1016/j.bsheal.2023.03.002

Xiuli Sun , Huanhuan Lu , Yanqing Tie , Mengchuan Zhao , Ruiqing Zhang , Zhenlu Sun , Guohao Fan , Fengyu Li , Fengyu Tian , Yaxin Hu , Mengyi Zhang , Xinxin Shen , Xuejun Ma , Zhishan Feng

{"title":"A one-step reverse-transcription recombinase aided PCR assay for the rapid and sensitive detection of human enteroviruses","authors":"Xiuli Sun , Huanhuan Lu , Yanqing Tie , Mengchuan Zhao , Ruiqing Zhang , Zhenlu Sun , Guohao Fan , Fengyu Li , Fengyu Tian , Yaxin Hu , Mengyi Zhang , Xinxin Shen , Xuejun Ma , Zhishan Feng","doi":"10.1016/j.bsheal.2023.03.002","DOIUrl":"10.1016/j.bsheal.2023.03.002","url":null,"abstract":"<div><p>Human enteroviruses (HEVs) include many different types that cause a wide range of diseases, and an effective method of genus-level identification has therefore significant clinical implications. However, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), the gold-standard method, still has shortfalls in diagnostic sensitivity and timeliness. Here we established a one-step real-time reverse-transcription recombinase-aided PCR assay (RT-RAP) to detect HEV fragment within an hour. The RT-RAP assay showed a detection limit of 5 copies/μL using recombinant plasmids and was extensively verified using 15 HEV strains. Among 15 types of HEV (species A-C), the sensitivity of RT-RAP was approximately 2–8 folds lower than that of the qRT-PCR in 9 types, and no-cross reaction with other viruses was observed. RT-RAP was further applied to analyze CSF and fecal specimens; the clinical performance demonstrated that the RT-RAP and the commercial qRT-PCR kit provided consistent results. These results indicated that RT-RAP assay may be a promising approach for rapid and sensitive detection of HEV.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 2","pages":"Pages 126-131"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47816143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards precision medicine: Omics approach for COVID-19 迈向精准医学:治疗COVID-19的组学方法

Biosafety and Health Pub Date : 2023-04-01 DOI: 10.1016/j.bsheal.2023.01.002

Xiaoping Cen , Fengao Wang , Xinhe Huang , Dragomirka Jovic , Fred Dubee , Huanming Yang , Yixue Li

引用次数: 4

Rapid evaluation of heterologous chimeric RBD-dimer mRNA vaccine for currently-epidemic Omicron sub-variants as booster shot after inactivated vaccine 目前流行的异源嵌合rbd -二聚体mRNA疫苗作为灭活疫苗后加强剂的快速评价

Biosafety and Health Pub Date : 2023-04-01 DOI: 10.1016/j.bsheal.2023.02.002

Qian Chen , Pei Du , Yuxuan Han , Xuehui Ma , Rong Zhang , Xiaoyu Rong , Xu Zhao , Renyi Ma , Huiting Yang , Anqi Zheng , Qingrui Huang , Jinghua Yan , Hui Wang , Xin Zhao , Lianpan Dai , George F. Gao , Qihui Wang

{"title":"Rapid evaluation of heterologous chimeric RBD-dimer mRNA vaccine for currently-epidemic Omicron sub-variants as booster shot after inactivated vaccine","authors":"Qian Chen , Pei Du , Yuxuan Han , Xuehui Ma , Rong Zhang , Xiaoyu Rong , Xu Zhao , Renyi Ma , Huiting Yang , Anqi Zheng , Qingrui Huang , Jinghua Yan , Hui Wang , Xin Zhao , Lianpan Dai , George F. Gao , Qihui Wang","doi":"10.1016/j.bsheal.2023.02.002","DOIUrl":"10.1016/j.bsheal.2023.02.002","url":null,"abstract":"<div><p>With continuous mutations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the severe immune escape of Omicron sub-variants urges the development of next-generation broad-spectrum vaccines, especially as booster jabs after high-level vaccination coverage of inactivated vaccines in China and many other countries. Previously, we developed a coronavirus disease 2019 (COVID-19) protein subunit vaccine ZF2001® based on the tandem homo-prototype receptor-binding domain (RBD)-dimer of the SARS-CoV-2 spike protein. We upgraded the antigen into a hetero-chimeric prototype (PT)-Beta or Delta-BA.1 RBD-dimer to broaden the cross-protection efficacy and prove its efficiency with protein subunit and mRNA vaccine platforms. Herein, we further explored the hetero-chimeric RBD-dimer mRNA vaccines and evaluated their broad-spectrum activities as booster jabs following two doses of inactivated vaccine (IV) in mice. Our data demonstrated that the chimeric vaccines significantly boosted neutralizing antibody levels and specific T-cell responses against the variants, and PT-Beta was superior to Delta-BA.1 RBD as a booster in mice, shedding light on the antigen design for the next-generation COVID-19 vaccines.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 2","pages":"Pages 89-100"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9425351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Laboratory diagnosis of nonpolio enteroviruses: A review of the current literature 非脊髓灰质炎肠道病毒的实验室诊断：现有文献综述

Biosafety and Health Pub Date : 2023-04-01 DOI: 10.1016/j.bsheal.2022.12.002

Tarek Itani , Vladislav Chalapa , Aleksandr Semenov , Aleksandr Sergeev

引用次数: 0