Journal of Functional Foods最新文献

{"title":"Unraveling amino acid sequence features that confer high calcium-binding capacity to soybean peptides and improve absorption in caco-2 cells","authors":"Jiulong An ,&nbsp;Manqun Wang ,&nbsp;Wanlu Liu ,&nbsp;Yumei Wang ,&nbsp;Na Ma ,&nbsp;Mingzhen Zhang ,&nbsp;Lu Li ,&nbsp;Chenchen Liu ,&nbsp;Xinqi Liu ,&nbsp;He Li","doi":"10.1016/j.jff.2025.106898","DOIUrl":"10.1016/j.jff.2025.106898","url":null,"abstract":"<div><div>Structure–activity relationship of food-derived peptides is a hotspot for nutrient interactions. Some studies have also improved the ability of these chelates to promote calcium absorption. However, whether the structures enabling calcium binding also influence absorption has not been studied in detail. Our previous research found that structural features, such as aspartic acid near the center of the N-terminal or consecutive aspartic acids, enhancing peptide calcium-binding capacity. Although the GGDLVS peptide exhibits these features, its effect on calcium absorption remains unclear. This study investigated chelates derived from peptides with or without these structures. The results indicated that chelates with this structure more effectively promoted Caco-2 cell calcium absorption and upregulated calcium channel protein TRPV6 expression. Molecular docking showed that the structure promoted protein interaction, facilitating calcium accumulation at the channel entry. Molecular dynamics simulations showed that the chelate structure promoted protein activation, stabilized conformation, and increased subsequent hydrogen bond formation.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"130 ","pages":"Article 106898"},"PeriodicalIF":3.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible rose (Rosa rugosa cv. ‘Plena’) aqueous extract, a potential antibacterial agent against multidrug-resistant Helicobacter pylori with anticancer activities","authors":"Shuhan Luo ,&nbsp;Meiling Pan ,&nbsp;Yihang Xu ,&nbsp;Ruixue Wang ,&nbsp;Xiao Zhang ,&nbsp;Juan Liao ,&nbsp;Tianli Zheng ,&nbsp;Xiaofang Pei","doi":"10.1016/j.jff.2025.106902","DOIUrl":"10.1016/j.jff.2025.106902","url":null,"abstract":"<div><div>Food-grade aqueous extracts from edible plants has the potential to serve as alternative agent for preventing <em>H. pylori</em> infection, due to high safety and antibacterial effects. Therefore, we evaluated the anti<em>-H. pylori</em> effects of 17 edible plants. Our results revealed that the aqueous extract of <em>Rosa rugosa</em> cv. ‘Plena’ (RA) emerged as the most potent anti-<em>H. pylori</em> agent. RA altered the morphology of <em>H. pylori</em> from spiral rods into coccoid forms, damaged its ultrastructure, inhibited its motility and urease activity. Utilizing network pharmacology analysis and qPCR, the mechanisms by which RA inhibited the aberrant activation of <em>PI3K/AKT</em> signaling pathway in gastric epithelial cells were confirmed. In conclusion, this study is the first study to report the anti-<em>H. pylori</em> effect and underlying mechanisms of RA, as well as its potential for further development into a novel food-grade agent for preventing <em>H. pylori</em> infections.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"130 ","pages":"Article 106902"},"PeriodicalIF":3.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, identification, and anti-melanogenesis activity of royal jelly protein peptides","authors":"Jiayi Liang ,&nbsp;Chengliang Guo ,&nbsp;Zhiwen Li ,&nbsp;Jing Bi ,&nbsp;Ruiming Wang ,&nbsp;Piwu Li ,&nbsp;Xiaohui Yang","doi":"10.1016/j.jff.2025.106897","DOIUrl":"10.1016/j.jff.2025.106897","url":null,"abstract":"<div><div>Excessive melanogenesis leads to hyperpigmentation-related skin disorders. To develop safe and effective anti-melanogenic agents, an ultrafiltered fraction RJPH-1 (MW &lt; 3 kDa) with high tyrosinase (TYR) inhibitory activity was obtained from royal jelly protein hydrolysates by pepsin enzymolysis and ultrafiltration in this study. It was found that RJPH-1 effectively inhibited TYR activity and melanin production in B16-F10 cells and zebrafish, downregulating the expression of melanogenesis-related genes (<em>MITF</em>, <em>TYR</em>, <em>TRP-2</em>) through the MAPK signaling pathway. Furthermore, the most active F2 component of RJPH-1 was isolated by gel filtration chromatography and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Molecular docking showed that the bioactive peptide FDYDPKFT could establish stable hydrogen bonds with TYR to inhibit its activity. These findings indicate that RJPH-1 and its active peptides can be used as potential anti-melanogenic agents for skin whitening.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106897"},"PeriodicalIF":3.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A lactoferrin-derived peptide LFDP1 alleviates experimental NEC via blocking ACSL4-LPCAT3 axis","authors":"Boshi Yu ,&nbsp;Shuwen Yao ,&nbsp;Yanjie Chen ,&nbsp;Wenjuan Chen ,&nbsp;Shuping Han","doi":"10.1016/j.jff.2025.106900","DOIUrl":"10.1016/j.jff.2025.106900","url":null,"abstract":"<div><div>This study investigated the anti-necrotizing enterocolitis (NEC) effects of LFDP1, a lactoferrin-derived peptide previously identified as up-regulated in human milk from mothers of preterm infants. Using both in vivo and in vitro models, we evaluated LFDP1's potential to attenuate NEC development. In NEC rat pups, LFDP1 administration significantly reduced the severity of clinical symptoms and pathological features. At the cellular level, LFDP1 restored viability and enhanced migration and proliferation of IEC6 and FHC cells. Molecular analysis revealed that LFDP1 downregulated key NEC process indicators (TLR4, ACSL4, and LPCAT3) while upregulating protective factors (ZO-1 and GPX4). RNA sequencing of LFDP1-treated FHC cells identified 117 differentially expressed mRNAs, with pathway analysis highlighting the involvement of polyunsaturated fatty acid biosynthesis and fatty acid metabolism. Further investigation showed that LFDP1 suppressed ferroptosis by inhibiting the ACSL4-LPCAT3 signaling axis, a finding corroborated in human NEC intestinal samples. Notably, LFDP1 treatment reduced oxidative stress and modulated the expression of ferroptosis-related proteins in vitro. These findings suggest that LFDP1 alleviates experimental NEC by repressing polyunsaturated fatty acid production and inhibiting ferroptosis via the ACSL4-LPCAT3 pathway. Our study not only elucidates a potential mechanism for LFDP1's protective effects but also contributes to the broader understanding of how breast milk composition may protect newborns from diseases like NEC.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106900"},"PeriodicalIF":3.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardized hot water extract from the aerial parts of Cirsium setidens (Dunn) Nakai alleviates UVB-induced skin photoaging damage through the inhibition of MMP-1/3 and MAPK/AP-1 pathways in vitro and in vivo","authors":"Min-Ji Park ,&nbsp;Nho-Eul Song ,&nbsp;Hyeon-A Song ,&nbsp;Kyung-Sook Chung ,&nbsp;Hyo-Jin An ,&nbsp;Hee-Do Hong ,&nbsp;Sang Yoon Choi ,&nbsp;Young Kyoung Rhee ,&nbsp;Kyung-Tae Lee","doi":"10.1016/j.jff.2025.106890","DOIUrl":"10.1016/j.jff.2025.106890","url":null,"abstract":"<div><div>Repetitive exposure to ultraviolet (UV) radiation causes skin damage, leading to inflammation, wrinkles, and aging. Many natural products are being highlighted as anti-aging agents due to their accessibility and minor side effects. Here, we explored the photoprotective effects of a standardized hot water extract from the aerial parts of <em>Cirsium setidens</em> (Dunn) Nakai (CS). In Hs68 cells, CS treatment reduced UVB-induced cytotoxicity and inflammation. CS decreased matrix metalloproteinase (MMP-1, −3) expression by inactivating the MAPK/activator protein-1 (AP-1) signaling pathways and recovered pro-collagen type I levels by activating the Smad pathway. CS reduced ROS production and activated heme oxygenase-1 (HO-1)/nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway. In HR-1 mice, oral administration of CS reduced wrinkle formation and epidermal/dorsal thickness, while also restoring hydration. CS inhibited collagen degradation by downregulating MMP-1, −3, and − 9 expression via inactivation of the MAPK/AP-1 and stimulation of the transforming growth factor beta (TGF-β)/Smad signaling pathways.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106890"},"PeriodicalIF":3.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative carnitine-fed rats model reveals resveratrol butyrate Ester's multimechanistic role in reducing TMAO and cardiovascular risk","authors":"Chih-Yao Hou ,&nbsp;Cai-Sian Liu ,&nbsp;Ming-Kuei Shih ,&nbsp;Asif Ali Bhat ,&nbsp;You-Lin Tain ,&nbsp;Chang-Wei Hsieh ,&nbsp;Yu-Wei Chen ,&nbsp;Shin-Yu Chen","doi":"10.1016/j.jff.2025.106910","DOIUrl":"10.1016/j.jff.2025.106910","url":null,"abstract":"<div><div>Trimethylamine-N-oxide (TMAO), a gut microbiota-derived metabolite from dietary carnitine, is a risk factor for cardiovascular disease (CVD). High-fat and carnitine-enriched diets increase TMAO levels, making them suitable for modeling diet-induced CVD. Resveratrol butyrate ester (RBE) and its monomer 3-O-butanoylresveratrol (ED4) have shown potential in reducing TMAO levels and improving cardiovascular outcomes, but their mechanisms remain unclear. This study established a novel rat model with high-fat and carnitine-enriched diets to elevate serum TMAO levels, simulating diet-induced CVD risks, and investigated the therapeutic effects of ED4. Physiological, biochemical, microbial, and gene expression profiles were analyzed. ED4 supplementation reduced serum TMAO levels by upregulating organic cation transporter novel family member 2 (OCTN2) expression, promoting urinary TMAO excretion, and restoring short-chain fatty acid (SCFA) levels. ED4 also modulated gut microbiota, reducing TMA-producing bacteria, and improved cardiovascular markers, including lowered blood lipid levels and fat accumulation. While dimethylbutanol inhibited flavin-containing monooxygenase 3 (FMO3) expression to reduce TMAO, ED4 acted primarily through carnitine utilization and microbiota modulation. Both treatments enhanced urinary TMAO excretion and altered gut microbiota composition. This study introduced an innovative animal model combining high-fat and carnitine-enriched diets to study TMAO-related cardiovascular risks. ED4 demonstrated multi-mechanistic effects in reducing TMAO levels and CVD risk factors by modulating gut microbiota, restoring SCFA levels, and enhancing carnitine metabolism. These findings highlight ED4's therapeutic potential in cardiovascular protection and metabolic regulation. However, further research is needed to elucidate the molecular pathways underlying ED4's effects on TMAO metabolism and its broader translational applications.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106910"},"PeriodicalIF":3.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed electric field-treated malvacin-3-O-galactoside and whey protein complexes alleviate silica-induced pulmonary fibrosis by promoting cellular mitochondrial autophagy and inhibiting cellular pyroptosis","authors":"Yancen Liu,&nbsp;Chuang Ma,&nbsp;Jinlong Tian,&nbsp;Bin Li,&nbsp;Jin Zhao","doi":"10.1016/j.jff.2025.106895","DOIUrl":"10.1016/j.jff.2025.106895","url":null,"abstract":"<div><div>Malvacin-3-<em>O</em>-galactoside (M3G) is the most abundant anthocyanins component in blueberries and has high biological activity. We investigated the effect of a pulsed electric field (PEF) on the interaction between whey protein (WP) and M3G. Stability experiments showed that PEF significantly improved stability of the anthocyanin-whey protein complex (M3G-WP), particularly at 15 kV/cm for 30 min. PEF-treated M3G-WP (PEF-M3G-WP) was used as a model of silica-induced lung fibrosis to investigate its anti-inflammatory activity and molecular mechanisms. Both <em>in vivo</em> and <em>in vitro</em> experiments demonstrated that PEF-M3G-WP alleviated inflammation in macrophages. It upregulated the expression of PINK1 and Parkin and downregulated the expression of NLRP3, CASP1, and GSDMD NT. Furthermore, qPCR experiments showed that PEF-M3G-WP inhibited the release of inflammatory factors IL1b and IL18 by upregulating Atg5 and downregulating P62 in alveolar macrophage cell lines (MH-S). In summary, PEF-treated M3G-WP may be a potential method for alleviating pulmonary fibrosis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106895"},"PeriodicalIF":3.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fortifying a meal with oyster mushroom powder on subsequent ad libitum energy intake, gastric emptying, selected gastrointestinal hormones and appetite sensations in adults with overweight/obesity: A double-blind randomized controlled crossover trial","authors":"Jana Johnen ,&nbsp;Lisa Dicks ,&nbsp;Dominik J. Kaczmarek ,&nbsp;Nils B. Kroemer ,&nbsp;Leonie Weinhold ,&nbsp;Sabine Ellinger","doi":"10.1016/j.jff.2025.106907","DOIUrl":"10.1016/j.jff.2025.106907","url":null,"abstract":"<div><div>This study aimed to investigate the effect of fortifying a meal with β-glucan-rich oyster mushroom <em>(Pleurotus ostreatus [P.o.])</em> powder on subsequent <em>ad libitum</em> energy intake and potential mechanisms modulating food intake. In a double-blind randomized, crossover trial, 36 adults consumed a meal enriched with 20 g <em>P.o.</em> powder (EN) or not (control, CON). Before and within 240 min afterward, gastric emptying, biochemical parameters (peptide YY, cholecystokinin, short-chain fatty acids) and appetite sensations were determined. <em>Ad libitum</em> energy intake was assessed after 240 min (second meal test). Gastric emptying was delayed after EN vs. CON (lag-time: <em>P</em> = 0.028; half-emptying time: <em>P</em> &lt; 0.001). For biochemical parameters, appetite sensations and subsequent <em>ad libitum</em> energy intake, no treatment effect was detectable (all <em>P</em> ≥ 0.05). Meal fortification with 20 g <em>P.o</em>. powder delays gastric emptying but an effect on <em>ad libitum</em> energy intake could not be detected under the experimental conditions applied.</div><div>Trial Registration</div><div>Registration at the German Clinical Trials Register; DRKS-ID: DRKS00031958; registration date: 25/05/2023. <span><span>https://drks.de/search/en/trial/DRKS00031958</span><svg><path></path></svg></span></div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106907"},"PeriodicalIF":3.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ziziphi Spinosae semen extract ameliorates insomnia by regulating hypothalamic GABA/Glu balance and gut microbiota Lactobacillus johnsonii","authors":"Zhenhua Bian ,&nbsp;Wenming Zhang ,&nbsp;Zifang Feng ,&nbsp;Lihong Gu ,&nbsp;Ping Qin ,&nbsp;Yingying Lu ,&nbsp;Xiaowei Chen ,&nbsp;Minmin Hu ,&nbsp;Liangyu Cai ,&nbsp;Lianlin Su ,&nbsp;De Ji ,&nbsp;Lin Li ,&nbsp;Shile Huang ,&nbsp;Tulin Lu","doi":"10.1016/j.jff.2025.106911","DOIUrl":"10.1016/j.jff.2025.106911","url":null,"abstract":"<div><div>Ziziphi Spinosae Semen (ZSS) is a notable seed food recognized for its sedative and sleep-promoting effects. ZZS improved sleep status and cognitive ability, and attenuated neuronal damage in the PCPA-induced insomnia rats. ZSS improved hypothalamic GABA/Glu balance by elevating GABA levels and GABA/Glu ratio, while lowering Glu levels. ZSS significantly increased expression of GAD67, GABRA1, and GABRG2, and decreased expression of GluR1, NMDAR1, and mGluR5. Notably, <em>L. johnsonii</em> emerged as one of the most significantly enriched microbial species following ZSS treatment, and administration of L. <em>johnsonii</em> showed effects similar to ZSS in improving GABA/Glu balance. Finally, anti-insomnia effects of ZSS were transferable to recipients through fecal microbiota transplantation, while ZSS treatment failed to ameliorate insomnia with depleted gut microbiota. This research validated the anti-insomnia effects of ZSS, along with its regulatory effects on GABA/Glu balance and gut microbiota, offering potential benefits for its utilization in the functional food sector.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106911"},"PeriodicalIF":3.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Marine algae oils from Phaeodactylum tricornutum and Laminaria japonica alleviate obesity, insulin resistance, and gut microbiota dysbiosis in high-fat diet-fed mice” [J. Funct. foods 117 (2024) 106234]","authors":"Lei Lü ,&nbsp;Shu-Xian Li ,&nbsp;Xiao-Yun Li ,&nbsp;Ji-Rong Ma ,&nbsp;Jin-Xing He ,&nbsp;Han-Zhu Xing ,&nbsp;Chen Chen ,&nbsp;Kazuo Miyashita ,&nbsp;Yu-Hong Yang ,&nbsp;Lei Du","doi":"10.1016/j.jff.2025.106908","DOIUrl":"10.1016/j.jff.2025.106908","url":null,"abstract":"","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106908"},"PeriodicalIF":3.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}