Heat-inactivated Bifidobacterium longum ZFML006 extends lifespan in Caenorhabditis elegans via MAPK and insulin/IGF-1 signaling pathways

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-08-25 DOI:10.1016/j.jff.2025.106992

Ruonan Wang , Yimin Li , Gang Wang , Zihao Zhang , Yingjie Huang , Ran Tuo , Ruomeng Dong , Zhen Li , Xiangfeng Chen , Shuxun Liu , Qing Gu , Ping Li

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Abstract

The global burden of aging populations and age-related functional decline presents a major public health challenge. Although live Bifidobacterium exhibits anti-aging properties, its strict anaerobic requirements limit its utility, whereas heat-inactivated preparations may offer enhanced stability and functional activity. This study characterized Bifidobacterium longum (B. longum) ZFML006 and systematically evaluated its anti-aging efficacy in Caenorhabditis elegans (C. elegans) using heat-inactivated administration. Treatment resulted in a 30.64 % increase in lifespan, a 15.20 % reduction in fecundity, a 49.92 % decrease in lipofuscin accumulation, a 20.69 % improvement in thermotolerance, a 14.8 % increase in oxidative stress survival, and enhanced motility. Additionally, it led to 10.6 % lower Reactive Oxygen Species (ROS) levels and elevated Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GSH-Px) activities. Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) analysis revealed coordinated downregulation of daf-2 and akt-2, accompanied by the upregulation of daf-16, sod-3, pmk-1, nsy-1, skn-1, and sek-1, confirming the modulation of both Insulin/IGF-1 signaling (IIS) and Mitogen-Activated Protein Kinase (MAPK) signaling pathways. These findings establish heat-inactivated ZFML006 as a potent anti-aging intervention by precisely regulating conserved longevity mechanisms.

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热灭活长双歧杆菌ZFML006通过MAPK和胰岛素/IGF-1信号通路延长秀丽隐杆线虫的寿命

人口老龄化和与年龄有关的功能衰退的全球负担是一项重大的公共卫生挑战。虽然活的双歧杆菌具有抗衰老的特性，但其严格的厌氧要求限制了它的应用，而热灭活制剂可能提供更高的稳定性和功能活性。本研究对长双歧杆菌（B. longum） ZFML006进行了特征鉴定，并采用热灭活方法系统评价了其对秀丽隐杆线虫（C. elegans）的抗衰老作用。处理后，寿命延长30.64%，繁殖力降低15.20%，脂褐素积累减少49.92%，耐热性提高20.69%，氧化应激存活率提高14.8%，运动能力增强。此外，它还导致活性氧（ROS）水平降低10.6%，过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性升高。逆转录定量聚合酶链反应（RT-qPCR）分析显示daf-2和akt-2协同下调，同时daf-16、sod-3、pmk-1、ny -1、skn-1和sek-1上调，证实了胰岛素/IGF-1信号通路（IIS）和丝裂原活化蛋白激酶（MAPK）信号通路的调节。这些发现证实热失活的ZFML006是一种有效的抗衰老干预剂，通过精确调节保守的长寿机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.