Sudebno-Meditsinskaya Ekspertisa最新文献

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[On the relevance of examining the internal organs for the presence of diatom plankton after drowning at sea].
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246706148
V M Antipov, I A Tolmachev, O S Lavrukova, A V Antipov, I Yu Kostikov
{"title":"[On the relevance of examining the internal organs for the presence of diatom plankton after drowning at sea].","authors":"V M Antipov, I A Tolmachev, O S Lavrukova, A V Antipov, I Yu Kostikov","doi":"10.17116/sudmed20246706148","DOIUrl":"https://doi.org/10.17116/sudmed20246706148","url":null,"abstract":"<p><strong>Objective: </strong>To establish the relevance of examining the internal organs for the presence of diatom plankton after drowning in the waters of the White Sea.</p><p><strong>Material and methods: </strong>We studied the biological material (fragments of lungs and kidneys) from the corpses of people found in the waters of the Kemskaya Bay and Onega Bay of the White Sea and waters from different areas of the White Sea for the presence of diatoms.</p><p><strong>Results: </strong>In all cases of drowning in the waters of the White Sea (in the area of the city of Belomorsk and the Kem skerries), marine and freshwater diatoms were found in the lungs and kidneys of the deceased in quantities sufficient to diagnose death from drowning in water. No diatoms were found in the unmixed waters of the White Sea. However, marine and freshwater diatoms were found in mixed waters (mouths of the Nizhniy Vyg River and the 19th lock of the White Sea-Baltic Canal). Desalination of seawater in the coastal zones of the White Sea reduces its salinity to isotonic values and even lowers it. This promotes the penetration of marine and freshwater diatoms contained in the water into the bloodstream of victims dying from drowning.</p><p><strong>Conclusions: </strong>The obtained results justify the relevance of examining the internal organs in cases of drowning in the coastal waters of the White Sea.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 6","pages":"48-51"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Study of the features of perchlozon isolation from biological fluids for the purposes of chemico-toxicological analysis].
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246706138
S S Konovalova, E A Illarionova, N V Chmelevskaya
{"title":"[Study of the features of perchlozon isolation from biological fluids for the purposes of chemico-toxicological analysis].","authors":"S S Konovalova, E A Illarionova, N V Chmelevskaya","doi":"10.17116/sudmed20246706138","DOIUrl":"https://doi.org/10.17116/sudmed20246706138","url":null,"abstract":"<p><strong>The study objective: </strong>Is to develop a methodology for extraction, identification and quantification of perchlozon from biological fluids for the purposes of chemico-toxicological analysis. The article describes the selection of optimal conditions for sample preparation, allowing to extract the perchlozon most effectively. The methodology is tested on model mixtures of biological fluids (urine, saliva, blood plasma). Identification and quantification were performed using UV spectrophotometry and high-performance liquid chromatography.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 6","pages":"38-41"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[On the prospects of the impedance monitoring method for determining the prescription of death coming]. [论阻抗监测法确定死亡来临处方的前景]。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236602120
V L Popov, E L Kazakova, O S Lavrukova, A Yu Polyakov
{"title":"[On the prospects of the impedance monitoring method for determining the prescription of death coming].","authors":"V L Popov,&nbsp;E L Kazakova,&nbsp;O S Lavrukova,&nbsp;A Yu Polyakov","doi":"10.17116/sudmed20236602120","DOIUrl":"https://doi.org/10.17116/sudmed20236602120","url":null,"abstract":"<p><p>The aim of the scientific work is to analyze the prospects of using the impedance monitoring method to determine the prescription of death coming. The conducted exploratory analysis allows hypothesizing the dependence of impedance values and dispersion factors for the diagnostic zones studies on the postmortem interval; it also suggests that it is possible to determine this interval for the objects (pig corpses) studies with a combination of impedance values and the corresponding factors. Among large mammals, a swine is the closest to a human from the point of analyzing the postmortem period process, and it can be recommended as an adequate human corpse model. When determining the correlation between the postmortem interval and the impedance parameters, it is the simplicity and reproducibility of the method, the absence of expensive equipment, its portability and the rapid receipt of results that allow this method to be applied at the scene, reinforcing traditional forensic methods for determining the prescription of death coming. Interpretation of impedance monitoring results can be used to analyze the biological nature of the postmortem period processes.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 2","pages":"20-25"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Practical value of microorganisms for forensic purposes (on the example of microbial flora of bony remnants from the historic burial site)]. [微生物在法医鉴定中的实用价值(以历史墓葬遗骨的微生物菌群为例)]。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236602130
N A Sidorova, O S Lavrukova, R F Beraya, V L Popov
{"title":"[Practical value of microorganisms for forensic purposes (on the example of microbial flora of bony remnants from the historic burial site)].","authors":"N A Sidorova,&nbsp;O S Lavrukova,&nbsp;R F Beraya,&nbsp;V L Popov","doi":"10.17116/sudmed20236602130","DOIUrl":"https://doi.org/10.17116/sudmed20236602130","url":null,"abstract":"<p><p>Phenotypic signs of dominants isolated from the surface of bony remnants from the historic burial site were analyzed in order to expand data on the biodiversity of microorganisms in the microbial flora of bony remnants and to assess the possibility of using the results of microbiological analysis in the evidence base of forensic examination and forensic archaeology. It was detected that only <i>Deuteromycota</i> and <i>Eubacteria</i> colonized all types of surfaces in the samples of bone fragments from the historic burial site (with the age in the range of 90-95 years); with the abundance of micromycetes, the proportion of Eubacteria naturally decreased, while with the increased bacterial background counts the rate of micromycetes detection decreased. The insignificant amount of nutrients in the bony remnants led to the decrease in the number and biological diversity of microorganisms contaminating them; species adapted to a hard-to-reach organic substrate dominated there. During the process of bony remnants decomposition, when the conditions of their location changed, inter-species competition and specific recolonization occurred by species of microorganisms most adapted to a hard-to-reach organic substrate in the abiotic and biotic conditions of existence given. The results obtained are important for the descriptive ecology and biology of specific groups of microorganisms in the postmortem microbiome and form the basis for a more thorough study of complex communications between species of microorganisms in the necrobiome of bony remnants - in the future it will allow putting forward original hypotheses about the involvement of microbes in the circulation of matter and energy, as well as to apply the information obtained in the evidence base of forensic examination and forensic archaeology.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 2","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Mathematical modeling of the fracture along the length of the femur diaphysis]. [沿股骨骨干长度骨折的数学模型]。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236604119
M A Kislov, K N Krupin, Yu I Pigolkin
{"title":"[Mathematical modeling of the fracture along the length of the femur diaphysis].","authors":"M A Kislov,&nbsp;K N Krupin,&nbsp;Yu I Pigolkin","doi":"10.17116/sudmed20236604119","DOIUrl":"https://doi.org/10.17116/sudmed20236604119","url":null,"abstract":"<p><strong>The aim of the study: </strong>Was to establish a pattern of femur diaphysis fracture with impact force over the entire front surface in an increments of 25 mm. Transverse, oblique and comminuted femur fractures were studied as a result of mathematical modeling. The application of mathematical modeling using the finite element analysis made it possible to visualize and predict the tension arising in the transient material during the impact force of blunt object, as well as the features of fractures' morphology in different sections of femur diaphysis. Modelled data about the mechanism and morphology of femur fracture were confirmed by the results of original full-scale experiments.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 4","pages":"19-24"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9872644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Chromatographic analysis of hard tooth tissue to determine the age of personality]. 【硬牙组织色谱分析确定人格年龄】。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236604158
P S Velenko, M P Poletaeva, A A Bychkov, D A Sheptulin, E A Gavrilova
{"title":"[Chromatographic analysis of hard tooth tissue to determine the age of personality].","authors":"P S Velenko, M P Poletaeva, A A Bychkov, D A Sheptulin, E A Gavrilova","doi":"10.17116/sudmed20236604158","DOIUrl":"10.17116/sudmed20236604158","url":null,"abstract":"<p><p>The biochemical approach for age assessment is most appropriate in forensic medicine, as racemization of aspartic acid in bones and teeth is closely related to human biological age. The aim of the study is to assess the biochemical parameters of aspartic acid in human teeth, which can be implemented into forensic practice in Russia. Samples of dentin in amount of 20, taken from the teeth of subjects aged between 16 and 76, were examined. Chromatographic analysis of the samples was performed on a gas chromatograph using chiral column. Statistical data processing showed that the relative squared peak of D-aspartic acid has a strong correlation with human biological age. Data, obtained from the Russian population study, demonstrate the applicability of chromatography for forensic purposes. It should be noted that the approach to the racemization rate estimation in the hard tooth tissue was performed using standard laboratory equipment, which allows to easily implement this method in forensic medical practice.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 4","pages":"58-61"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9872647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Ways to solve the problem of cross-reactions during immunochromatographic examination of biological objects]. 【解决生物物体免疫层析检查时交叉反应问题的方法】。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236601143
P S Vikman, A S Zhuravleva, O Yu Strelova, A N Grebenyuk
{"title":"[Ways to solve the problem of cross-reactions during immunochromatographic examination of biological objects].","authors":"P S Vikman,&nbsp;A S Zhuravleva,&nbsp;O Yu Strelova,&nbsp;A N Grebenyuk","doi":"10.17116/sudmed20236601143","DOIUrl":"https://doi.org/10.17116/sudmed20236601143","url":null,"abstract":"<p><p>The purpose of this review is to study the causes of cross-reactions of a number of drugs (mebeverine, phenibut, tropicamide, ramipril, metoprolol, phenylephrine, sertraline, chloropyramine and diphenhydramine) during the preliminary stage of laboratory diagnostics by immunochromatographic method and to propose a possible algorithm for solving this problem. Conducting a hair study in order to identify the fact of the use of psychoactive substances will increase the reliability of analytical diagnostics and reduce the likelihood of false positive results of the analysis. The use of a validated method of enzymatic hydrolysis of hair will eliminate unreliable results of the analysis due to the detection of the native molecule of the toxicant, increase the efficiency and accuracy of the diagnostic procedure.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 1","pages":"43-49"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10602266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[TGFβ1 antibodies application for IHC determination of injuries vitality. The experimental results]. 【TGFβ1抗体在IHC损伤活力测定中的应用。实验结果】。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236605143
M V Fedulova, D D Kupriyanov
{"title":"[TGFβ1 antibodies application for IHC determination of injuries vitality. The experimental results].","authors":"M V Fedulova,&nbsp;D D Kupriyanov","doi":"10.17116/sudmed20236605143","DOIUrl":"https://doi.org/10.17116/sudmed20236605143","url":null,"abstract":"<p><strong>The aim of the study: </strong>Is to examine the applicability of IHC staining method with TGFβ1 antibodies for injuries vitality determination. 72 skin autopsy samples with mechanical injuries from 12 persons were divided to 3 groups (<i>n</i>=24 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC with TGFβ1 antibodies was performed. We concluded the positive TGFβ1 staining was in keratinocytes in vitally injured skin and the negative one in postmortally injured and control non-injured samples. The role of TGFβ1 in the injuries healing process and the further examinations of the TGFβ1 staining method application for determination of injuries vitality are discussed.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 5","pages":"43-46"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41131631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Lercanidipine distribution in warm-blooded animals]. [乐卡地平在温血动物中的分布]。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236605147
L L Kvachakhiya, V K Shormanov
{"title":"[Lercanidipine distribution in warm-blooded animals].","authors":"L L Kvachakhiya,&nbsp;V K Shormanov","doi":"10.17116/sudmed20236605147","DOIUrl":"10.17116/sudmed20236605147","url":null,"abstract":"<p><strong>The aim of the study: </strong>Is to investigate the lercadipine distribution in warm-blooded animals (rats). The experimental study used rats of Wistar race. TLC, GC-MS and UV-spectrophotometry methods were used for physical and chemical analysis. Semilethal (890 mg/kg) dose of lercadipine, previously suspended in water, was injected into stomach of laboratory animals. Examined substance was isolated from the thick tissues and animals' blood by acetone, cleaned with a change of solvent and macrocolumn chromatography using 30 µm «Silasorb S-18» sorbent and acetonitrile-water (8:2) polar eluent. The analyte was identified by chromatographic behavior in the thin sorbent layer, retention time and set of positive ions in its mass spectrum, as well as by UV-spectrum. The analyte was determined quantitatively in bioactive matrix using UV-spectrophotometry. The methods were validated by criteria of linearity, selectivity, accuracy, precision, detection limits and quantitative determination. The main content of lercanidipine (mg/100 g) was determined in the stomach content (198.183±29.541), the stomach (195.312±21.579), the small intestine (47.096±3.947), the spleen (38.952±3.532) and the liver (26.211±2.232).</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 5","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41137047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Distribution features of gunshot residue from hunting Steyr Mannlicher AUG-Z3 carbine on targets]. [狩猎用斯太尔曼利切 AUG-Z3 卡宾枪射击残留物在目标上的分布特征]。
Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI: 10.17116/sudmed20236606113
A N Prikhod'ko, S V Leonov, A D Surovikin
{"title":"[Distribution features of gunshot residue from hunting Steyr Mannlicher AUG-Z3 carbine on targets].","authors":"A N Prikhod'ko, S V Leonov, A D Surovikin","doi":"10.17116/sudmed20236606113","DOIUrl":"10.17116/sudmed20236606113","url":null,"abstract":"<p><strong>Objective: </strong>To study the morphology of distribution of shot factors on white targets made of coarse calico at different distances.</p><p><strong>Material and methods: </strong>The study was conducted on 57 experimental damages of targets made of cotton fabric, caused by shots from hunting self-loading Steyr Mannlicher AUG-Z3 carbine of.223 Rem caliber. The number of experimental firings equal 19 was done by 3 shots at the distance from close range to 250 cm. The examination of shot targets was carried out using the visual, metric, stereoscopic (Leica 125M microscope) methods, in ultraviolet rays (LUMATEC SUPERLITE M Series light).</p><p><strong>Results: </strong>The nature and morphology of distribution of gunshot residue on targets' surfaces made it possible to establish close range firing zones. The nature in which soot is deposited during shot under the conditions of point-blank range and in the first zone of close range (up to 8 cm) allows to establish the position of weapon's transverse plane relative to the target basing on a specific blowout of a gas-powder jet from special design of flame arrestor. The performed experimental shooting from hunting self-loading Steyr Mannlicher AUG-Z3 carbine made it possible to determine the metric and morphological characteristics of the close range firing zones: 1 zone is equal a distance up to 8 cm; 2 zone - up to 30 cm; 3 zone - up to 100 cm from muzzle of weapon. The design features of Steyr Mannlicher AUG-Z3 carbine's flame arrestor allow to identify the type of weapon according to the morphological pattern of target's damage. The complex of obtained data makes it possible to determine the firing distance with high accuracy.</p>","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"66 6","pages":"13-17"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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