Sudebno-Meditsinskaya Ekspertisa最新文献_第8页

[Development of selective method for identifying mebeverine in hair]. [开发鉴定毛发中美白藜芦醇的选择性方法]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246705129

P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk

{"title":"[Development of selective method for identifying mebeverine in hair].","authors":"P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk","doi":"10.17116/sudmed20246705129","DOIUrl":"https://doi.org/10.17116/sudmed20246705129","url":null,"abstract":"Objective: To develop the method for identification of mebeverine and its metabolites in biological materials (urine and hair) for solution of the problem of possible cross-reaction with psychoactive substances during preliminary studies by immunochromatographic method.Material and methods: The study was carried out using «Mebeverine» tablets (NAO «Severnaya Zvezda», Russia), enzymes, namely trypsin, chymotrypsin and hyaluronidase, papain. The obtained extracts were analyzed using HELC-MS/MS on a modular liquid Nexera XR chromatograph with a tandem LCMS-8050 (Shimadzu) mass spectrometer. Guinea pigs weighing about 500 gr were used in modelling the long-term use of mebeverine. The drug was administered per os, once to 5 guinea pigs at a dosage of 200 mg calculated as an animal weighing 500 gr and daily urine was collected. The wool was collected 30 days after regular administration.Results and conclusion: The main mebeverine's metabolites have been determined and native mebeverine and its marker metabolites (mebeverine and veratric acids) have been found in urine while taking this medicine in therapeutic doses for medical purposes. The application of enzymatic hydrolysis of hair method allowed to establish that hair tissue accumulates only a native molecule of mebeverine, therefore, only by results of hair analysis it is possible to conclude, if there was pharmacological use of this medicine or psychoactive substances from the group of phenylalkilamines. The effectiveness of enzymatic hydrolysis by hyaluronidase to isolate mebeverine from hair has been shown.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 5","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Review of age assessment methods of children and adolescents by teeth X-rays]. [儿童和青少年牙齿 X 射线年龄评估方法综述]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246702147

G V Zolotenkova, M P Poletaeva

引用次数: 0

[Comparative analysis of factors influencing suicidal actions performance before and during the COVID-19 pandemic]. [COVID-19大流行之前和期间影响自杀行动表现的因素比较分析]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed2024670115

M A Kislov, A N Prikhodko, E M Kildyushov, E N Artemieva, M M Marevichev, S A Stepanov, A S Shpikuleva

引用次数: 0

[Correlation between defects in medical care and data of causal analysis]. [医疗缺陷与因果分析数据之间的相关性]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246701162

A N Hovsepyan, K G Mkhitaryan

引用次数: 0

[Determination of 2,4-dimethylhydroxybenzene by chromatographic methods in forensic toxicological research of biological material]. [生物材料法医毒理学研究中的色谱法测定 2,4-二甲基羟基苯]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246701140

A P Chernova, O I Pugacheva, V K Shormanov, L O Orekhova, M V Shashkova

{"title":"[Determination of 2,4-dimethylhydroxybenzene by chromatographic methods in forensic toxicological research of biological material].","authors":"A P Chernova, O I Pugacheva, V K Shormanov, L O Orekhova, M V Shashkova","doi":"10.17116/sudmed20246701140","DOIUrl":"10.17116/sudmed20246701140","url":null,"abstract":"Objective: Is to develop a method for determining 2.4-dimethylhydroxybenzene (2.4-DMHOB) in biological material. The analytical methods used in the experiments were extraction, column chromatography of normal pressure, TLC, GC-MS and HPLC. To extract the analyte from the bioactive matrix, maceration with the binary insulating agent acetone-ethyl acetate (3:7) was used, observing the 2:1 mass ratio of the «insulating agent-matrix». Optimal conditions of semi-preparative analyte chromatography were achieved in column (150×10 mm) of «Silasbor» S-18 sorbent in elution with a mixture of acetonitrile-water (7:3), which was used in the proposed cleaning scheme, combining extraction and reversed-phase column chromatography. The application of the mobile phase of tetrachloromethane-dioxane (9.5:0.5) has been substantiated for the selective determination of 2.4-DMHOB by TLC («Sorbfil» plates). The expediency of confirming identification of the analyte in the form of 2.4-dimethyltrymethylsilylphenol using GC-MS (DB-5MS EVIDEX column (25.000×0.2 mm), stationary phase (5%-phenyl)-methylpolysiloxane, carrier gas - helium) has been shown. The group of characteristic particles in the mass spectrum of trimethylsilyl analyte derivative was represented by 45; 59; 73; 82; 91; 105; 119; 135; 149; 163; 179; 194 m/z ions. HPLC (Discovery C18 250×4.6 mm column, eluting liquid - acetate buffer solution with pH 5.5 - acetonitrile, 50:50) was used to confirm the identification and quantification of 2.4-DMHOB. A method for determining 2.4-DMHOB by the HPLC method in biological material (liver tissue) is proposed, which corresponds to the criteria of linearity, selectivity, accuracy, precision and stability. The minimum detectable quantity of 2.4-DMHOB in the bioactive matrix is 0.5 µg/g, the minimum determined quantity is 1.2 ug/g.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 1","pages":"40-46"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development of selective method for mebeverine detection in blood]. [开发血液中甲基比弗林的选择性检测方法]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246701134

P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk

{"title":"[Development of selective method for mebeverine detection in blood].","authors":"P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk","doi":"10.17116/sudmed20246701134","DOIUrl":"10.17116/sudmed20246701134","url":null,"abstract":"Objective: To develop the method of biological fluids' sample processing and mebeverine detection to exclude false results' receiving when diagnosing drug intoxication.Material and methods: The study was carried out using «Mebeverine» (NJCO «North star», Russia) medicine and hydrolysis by enzymes, namely papain, chymotrypsin, trypsin, chymopsin and hyaluronidase, was applied for sample processing. The extractions were analyzed by methods of HPLC-MS/MS on Nexera XR modular liquid chromatograph with LCMS-8050 (Shimadzu) tandem mass spectrometer and GC-MS on gas chromatograph connected with QP-2020 (Shimadzu, Japanese) mono quadrupole mass spectrometer.Results and conclusion: It has been revealed that using selective method of sample processing, which consists of aqueous phase extraction at pH=2-4 and enzymatic hydrolysis by papain and hyaluronidase during isolation from blood, is required to detect mebeverine in biological liquids. It has been proven that the native mebeverine is hydrolyzed to veratric (3.4-dimethoxybenzoic) acid and mebeverine alcohol at alkalotic pH value of medium. It has been shown that mebeverine extraction is necessary to study using HPLC-MS/MS, which will allow to avoid the native mebeverine degradation in chromatograph injector as with GC-MS method analysis.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 1","pages":"34-39"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Experience of Data Analysis application for proceeding research data in solving the problem of target age group establishment]. [数据分析应用于研究数据以解决目标年龄组建立问题的经验]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246704137

Yu I Pigolkin, V I Solodovnikov, D D Zolotenkov, B R Salem, M P Poletaeva, G V Zolotenkova

引用次数: 0

[Forensic age diagnosis by assessment of sternum ossification processes]. [通过评估胸骨骨化过程进行法医年龄诊断]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246704147

G V Zolotenkova, S V Shigeev, T A Tareeva, E K Vershinina

{"title":"[Forensic age diagnosis by assessment of sternum ossification processes].","authors":"G V Zolotenkova, S V Shigeev, T A Tareeva, E K Vershinina","doi":"10.17116/sudmed20246704147","DOIUrl":"https://doi.org/10.17116/sudmed20246704147","url":null,"abstract":"Objective: To study the sternum ossification processes that take place over time as well as the possibilities of sternum X-rays application to evaluate the age on the modern Russian population in forensic terms.Material and methods: Sternum X-rays with fragments of ribs' chondral parts were obtained from 369 male and female corpses aged 12-96 years without pathological and traumatic changes. The X-rays were done in frontal projection. The set of examined parameters included: complete and partial synostosis of manubrium and body, induration along the synostosis (or on the articulated edges of manubrium and body), complete and partial synostosis of body segments, traces of body segments union, complete and partial synostosis of body and xiphoid process, induration along the synostosis (or on the articulated edges of manubrium and body), form of costal notches, induration of articular edges of body, presence of calcification foci in sternal edge of II-VII ribs' cartilages, depth of sternum notches, overgrowths on articular edges of body, presence of union between body's costal notches and calcification centers of ribs' cartilages. Mean values, standard deviation and correlation coefficient were determined.Results: The maximal correlation with age was found in such parameters such: overgrowths on articular edges of body (r=0.6), presence of calcification foci in sternal edge of II-VII ribs (r=0.6), induration of body's articular edges (r=0.5), presence of union between costal notches and calcification centers of ribs cartilages (r=0.5), synostosis of body and xiphoid process (r=0.4).Conclusion: A subsequent construction of linear model for age diagnosis has its details when comparing the predicted age with the real.","PeriodicalId":35704,"journal":{"name":"Sudebno-Meditsinskaya Ekspertisa","volume":"67 4","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Possibilities of educational process and researches realization using organs and tissues of the deceased person]. [利用死者器官和组织进行教育和研究的可能性]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246702162

A L Kochoyan, M N Nagornov, D P Berezovskiy

引用次数: 0

[Characteristics of bloodstain patterns in bleeding from a contused wound of head with rupture of a branch of superficial temporal artery]. [头部挫伤伴颞浅动脉分支破裂出血的血痕特征]。

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI: 10.17116/sudmed20246706112

M N Nagornov, R V Kalinin, D V Karasev, P A Lomyga, E N Leonova

引用次数: 0