遗传最新文献_第2页

Molecular basis of microRNA stability and degradation in plants. 植物中microRNA稳定性和降解的分子基础。

遗传 Pub Date : 2025-08-01 DOI: 10.16288/j.yczz.25-030

Meng-Wei Guo, You-Hong Fan, Guo-Dong Ren

引用次数: 0

Application and prospects of current computational methods in m⁶A research: a comprehensive review. 当前计算方法在m6A研究中的应用与展望

遗传 Pub Date : 2025-08-01 DOI: 10.16288/j.yczz.24-373

Ding-Wei Lei, Rui-Chu Gu, Xiao-Xue Xie, Shi-Zhi Ding, Han Wen

{"title":"Application and prospects of current computational methods in m6A research: a comprehensive review.","authors":"Ding-Wei Lei, Rui-Chu Gu, Xiao-Xue Xie, Shi-Zhi Ding, Han Wen","doi":"10.16288/j.yczz.24-373","DOIUrl":"10.16288/j.yczz.24-373","url":null,"abstract":"N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic mRNA, playing a pivotal role in regulating various aspects of mRNA metabolism, including splicing, processing, degradation, and translation. This review provides a comprehensive overview of computational strategies employed in m6A research, with an emphasis on data-driven methodologies for the prediction of m6A sites and molecular dynamics simulations for deciphering m6A-associated biological mechanisms. The article first discusses the evolution of m6A detection technologies, outlines the corresponding data processing methods, and summarizes publicly available datasets that serve as essential resources for constructing computational models. Subsequently, we highlight research advancements in machine learning and deep learning models for m6A site prediction. Finally, we demonstrate the contributions of molecular dynamics simulations in unravelling m6A-related molecular mechanisms, illustrating how computational methods facilitate the understanding of this complex epigenetic regulation. By systematically synthesizing relevant content, this review further discusses the latest research progress and application values of computational methods in m6A modification, offering new perspectives and insights for in-depth investigations.","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"903-927"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogenesis, action, function of plant small RNAs and their potential application in agriculture. 植物小rna的生物发生、作用、功能及其在农业中的潜在应用。

遗传 Pub Date : 2025-08-01 DOI: 10.16288/j.yczz.25-163

Huai-Hao Yang, Bing-Lian Zheng

{"title":"Biogenesis, action, function of plant small RNAs and their potential application in agriculture.","authors":"Huai-Hao Yang, Bing-Lian Zheng","doi":"10.16288/j.yczz.25-163","DOIUrl":"10.16288/j.yczz.25-163","url":null,"abstract":"Plant small RNAs (sRNAs) are essential regulators of gene expression and genome stability in plants. Based on their biogenesis and mechanisms of action, they are primarily classified into two major categories: microRNAs (miRNAs) and small interfering RNAs (siRNAs). These sRNAs rely on distinct processing proteins for their production and effector proteins to execute their functions, playing pivotal roles in diverse developmental processes and environmental responses. Recent advances in next-generation sequencing have identified numerous novel sRNAs across multiple plant species, while studies in Arabidopsis thaliana and various crops have significantly enhanced our understanding of their biogenesis, regulatory networks, and biological functions. In this review, we systematically summarize the research progress on different classes of plant sRNAs, focusing on their biosynthetic pathways, molecular mechanisms, and biological function. Furthermore, we discuss the potential applications of plant sRNAs in agriculture, including their prospects as next-generation RNA pesticides, supported by current technological developments. This review aims to provide a theoretical foundation for further research on plant sRNAs and their agricultural applications.","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"928-943"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress on nucleos(t)idyl lipid-based nanoparticles for nucleic acid drugs delivery. 核酰基脂基核酸药物递送纳米颗粒研究进展。

遗传 Pub Date : 2025-08-01 DOI: 10.16288/j.yczz.24-378

Jia-Mei Hong, Hong-Yi Liu, Hua Guo, Jing Yu, Qi Zhang, Zhu Guan, Zhen-Jun Yang

{"title":"Progress on nucleos(t)idyl lipid-based nanoparticles for nucleic acid drugs delivery.","authors":"Jia-Mei Hong, Hong-Yi Liu, Hua Guo, Jing Yu, Qi Zhang, Zhu Guan, Zhen-Jun Yang","doi":"10.16288/j.yczz.24-378","DOIUrl":"10.16288/j.yczz.24-378","url":null,"abstract":"Nucleic acid drugs can function at the gene level, and have the advantages of simple synthesis, easy modification and high specificity. However, there are many obstacles in transfection and in vivo delivery due to their negative charge, high molecular weight, and hydrophilicity. Lipid nanoparticles (LNPs) can encapsulate siRNA or mRNA through electrostatic interactions and five related drugs have been approved as of April 2025. However, due to the inevitable immunogenicity and hepatosplenic toxicity, most LNP-encapsulated nucleic acid drugs were terminated in the early clinical stage. Nucleos(t)idyl lipids are a class of amphiphilic molecules composed of nucleobases or nucleos(t)idyl heads, linkers and lipid tail chains, which can bind with the bases of nucleic acid drugs through hydrogen bonding and π-π stacking and self-assemble to form nanoparticles or micelles with broad application prospects. In this review, we summarize the research progress in delivery systems of nucleic acid drugs based on nucleos(t)idyl lipids and peptidyl lipids, and discuss their differences with LNP-encapsulated nucleic acid drugs, including structural characterization, molecular dynamics simulation, in vivo distribution, as well as efficacy and safety, so as to provide new ideas for improving the targeting delivery of nucleic acid drugs.","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"823-841"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas system targeting RNA and its derivative technology. 靶向RNA的CRISPR/Cas系统及其衍生技术。

遗传 Pub Date : 2025-08-01 DOI: 10.16288/j.yczz.24-311

Xun Zhou, Shi-Jie Zhou, Jie Liu, Yu-Xiang Wang

引用次数: 0

RNAi-based antiviral immunity. 基于rnai的抗病毒免疫。

遗传 Pub Date : 2025-08-01 DOI: 10.16288/j.yczz.25-077

De-Yu Xu, Xi Zhou, Yu-Jie Ren

{"title":"RNAi-based antiviral immunity.","authors":"De-Yu Xu, Xi Zhou, Yu-Jie Ren","doi":"10.16288/j.yczz.25-077","DOIUrl":"10.16288/j.yczz.25-077","url":null,"abstract":"RNA interference (RNAi) is a gene silencing mechanism mediated by small RNAs derived from double-stranded RNA (dsRNA), capable of silencing specific genes. Following viral invasion, the dsRNA produced during viral replication is cleaved by the host cell's Dicer protein, generating virus-derived small interfering RNAs (virus-derived small interference RNAs, vsiRNA). These vsiRNAs then guide the cleavage and degradation of viral RNA via the RNAi pathway, exerting an antiviral effect. Therefore, RNAi is also recognized as an efficient antiviral immune pathway during viral infection. However, through long-term evolution, viruses have developed various strategies to counteract RNAi. For instance, they encode specific viral suppressors of RNAi (VSRs) that target and antagonize key molecules in this pathway. Research indicates that designing drugs to specifically target VSRs can \"unlock\" the antiviral function of RNAi within host cells, demonstrating highly potent and relatively broad-spectrum antiviral activity. Furthermore, viral infection can also be regulated by host- or virus-derived microRNAs (miRNAs). The role of miRNAs in viral infection provides new targets for antiviral therapy. In this review, we summarize the mechanism of RNAi in antiviral immunity, recent research advances, and its application prospects in antiviral therapy, aiming to provide theoretical support for antiviral immunity research and treatment.","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"876-884"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design strategies and applications of fluorescent protein-based probes. 基于荧光蛋白的探针设计策略及应用。

遗传 Pub Date : 2025-07-20 DOI: 10.16288/j.yczz.25-022

Ning Zhang, Ye Tian

引用次数: 0

Progress on the construction of Streptomyces minimized genomes. 链霉菌最小化基因组构建研究进展。

遗传 Pub Date : 2025-07-20 DOI: 10.16288/j.yczz.25-008

Hui Li, Li-Juan Guo, He-Xia Zhou, Chun-Yang Li, Jia-Lin Dai, Li-Zhu Shen, Rui Zhang, Feng-Hui Sun

引用次数: 0

Potential value of anoikis transcriptional signatures in predicting prognosis and immune microenvironment in hepatocellular carcinoma. anoikis转录特征在预测肝细胞癌预后和免疫微环境中的潜在价值。

遗传 Pub Date : 2025-07-20 DOI: 10.16288/j.yczz.25-007

Fu-Rong Cheng, Wen-Yu Song, Peng-Bo Cao, Gang-Qiao Zhou

{"title":"Potential value of anoikis transcriptional signatures in predicting prognosis and immune microenvironment in hepatocellular carcinoma.","authors":"Fu-Rong Cheng, Wen-Yu Song, Peng-Bo Cao, Gang-Qiao Zhou","doi":"10.16288/j.yczz.25-007","DOIUrl":"https://doi.org/10.16288/j.yczz.25-007","url":null,"abstract":"Resistance to anoikis, a crucial factor in cancer cell survival, drives the development and progression of numerous malignancies. Hepatocellular carcinoma (HCC) is a malignant liver tumor characterized by high rates of recurrence and metastasis. However, the role of anoikis in HCC remains poorly understood. In this study, we identify 74 anoikis-related genes (ARGs) differentially expressed in HCC using the transcriptional data from The Cancer Genome Atlas (TCGA). Then, we develop a prognostic model incorporating 9 of these genes through LASSO-Cox regression analysis, and confirm the model's independent prognostic significance for overall survival in HCC patients by using multivariable Cox proportional hazards analysis. Furthermore, we observe significant enrichment of activated proliferation-related pathways, increased infiltration of immunosuppressive cells and resistance to anti-PD-L1 therapy in the high-risk group defined by this model. These findings suggest that the ARG model may serve as a novel prognostic indicator for HCC patients and underscore the critical role of anoikis in HCC progression.","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 7","pages":"768-785"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in microRNA promoting gene expression. microRNA促进基因表达的研究进展。

遗传 Pub Date : 2025-07-20 DOI: 10.16288/j.yczz.24-374

Xiao Zhang, Yan Yu, Yong Ning, Qi-Wen Hong, Huai-Ping Shi

引用次数: 0