遗传最新文献

{"title":"Construction and analysis of molecular genetic map of perennial Chinese rice.","authors":"Ting-Shen Peng, Jiu-Yan Lu, Yu-Xin Yan, Lin Tan, Wen-Bin Nan, Xiao-Jian Qin, Ming Li, Jun-Yi Gong, Yong-Shu Liang","doi":"10.16288/j.yczz.24-340","DOIUrl":"https://doi.org/10.16288/j.yczz.24-340","url":null,"abstract":"<p><p>To develop perennial rice varieties and realize one planting (year) more harvest planting pattern of increasing yield and farmer's income is one of the most cost-effective strategy involved in safeguarding China's grain supply. In this study, construction and analysis of molecular maps of perennial rice was performed to elucidate the genetic laws of microsatellite loci in perennial Chinese rice, two half-sib F₂ populations derived from two perennial Chinese <i>japonica</i> rice (HN2^# and CB7^#) crossed to the annual <i>indica</i> rice XieqingzaoB (XQZB) were developed to construct two half-sib linkage maps. We established linkage map lengths of 2,036.10 cM and 1,878.23 cM with average genetic distance of 18.85 cM and 17.23 cM by using 108 and 109 SSR markers in both HN2^# and CB7^# map, respectively. Chi-square value (<i>χ</i>²) for genotypes in the F₂ populations of both HN2^# and CB7^# were 134.85 and 291.02, respectively, and exhibited extreme significant bias towards XQZB. <i>χ</i>² value for genotype on each linkage group of both HN2^# and CB7^# map ranged from 2.23 to 175.67, from 4.53 to 191.52, respectively. Genotypes on linkage groups of both the 1^st, 2^nd, 3^rd, 4^th, 6^th, 8^th, 9^th, 10^th, and 12^th in HN2^# map and 1^st, 2^nd, 3^rd, 5^th, 6^th, 7^th, 9^th, 11^th, and 12^th in CB7^# map deviated from the Mendelian ratio. There 38 F₂ individual in HN2^# and 47 F₂ individual in CB7^# population deviated from the Mendelian ratio, respectively. Altogether 32 markers showed segregation distortion (29.63%) and clustered on the 3^rd, 4^th and 6^th of linkage in HN2^# map, there 44 markers showed segregation distortion (40.37%) and clustered on the 3^rd, 5^th, 6^th, 7^th, 9^th, and 12^th of linkage in CB7^# map. Overall, this study lays a good foundation for the mining of beneficial genes and the innovation and utilization of perennial Chinese rice genetic resources.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"1042-1056"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental regulatory factors promote the efficiency of crop genetic transformation.","authors":"Ying-Ying Xie, Ke-Jian Wang, Yu-Chun Rao, Yong Huang","doi":"10.16288/j.yczz.24-345","DOIUrl":"https://doi.org/10.16288/j.yczz.24-345","url":null,"abstract":"<p><p>Genetic transformation technology is a core tool for generating plants with gain- or loss-of-function in modern biology and agricultural biotechnology. In recent years, developmental regulatory factors (DRs) have shown great potential in enhancing the efficiency of crop genetic transformation. In this review, we systematically examine the main crop genetic transformation methods, including <i>Agrobacterium</i>-mediated transformation and gene gun techniques, and discuss the current challenges in the transformation process, such as low transformation efficiency, strong genotype dependence, and insufficient regeneration capacity. We then provide a detailed discussion of several key developmental regulatory factors and their recent applications in improving transformation efficiency, overcoming genotype dependence, and enhancing regeneration capacity. Additionally, we explore the future prospects of DRs, highlighting their significant potential applications in precision gene editing, functional genomics, synthetic biology, and crop genetic improvement. Through in-depth research on DRs, the bottlenecks in crop genetic transformation are expected to be effectively overcome, thus advancing the further development of crop science and agricultural biotechnology.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"992-1006"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prognostic risk model construction for endometrial cancer based on inflammation-related genes.","authors":"Jian-Yao Liu, Yue Li, Huan-Huan Hu, Shu-Yue Xiao, Xin-Yi Xie, Shan-Liang Zhong, Zhen Gong, Chen-Jing Zhu, Han-Zi Xu","doi":"10.16288/j.yczz.24-376","DOIUrl":"https://doi.org/10.16288/j.yczz.24-376","url":null,"abstract":"<p><p>Inflammatory responses have been identified as a critical factor in the development and progression of various types of tumors. These responses influence the tumor microenvironment, promoting tumor cell invasion and migration while concomitantly reducing the efficacy of tumor therapy. Inflammation is widely regarded as a significant risk factor for the development of endometrial cancer (EC). However, the precise mechanisms through which it influences the development of EC remain to be elucidated. In this study, we obtain RNA expression profiles of EC patients and related clinical information from The Cancer Genome Atlas (TCGA) database. We then screen key inflammation-related genes using survival analysis and the least absolute value shrinkage and selection operator (LASSO) algorithms. Based on this, we finally construct a prognostic risk scoring model containing nine non-zero coefficient IRGs and an alignment diagram prediction model. Survival analysis demonstrates that patients in the low-risk group exhibit a higher survival rate and more favorable prognosis. The predictive performance of both models was confirmed through the analysis of test sets and calibration curves. Subsequently, we obtain EC-related datasets from the Gene Expression Omnibus (GEO) database to serve as an external validation, thereby further substantiating the reliability of the models. Subsequent immune infiltration analysis revealed significant disparities among nine immune cell types between the high- and low-risk groups, with multiple immune cells correlating with tumor progression and prognosis. Concurrently, we perform drug sensitivity analysis, it reveals a significant correlation between one representative EC drug, tamoxifen, and one of the aforementioned IRGs. In summary, our study successfully constructs a risk score model and a column-line graph prediction model for EC. It is expected that these models will better predict the overall survival and provide new therapeutic targets for EC patients.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"1007-1022"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The molecular mechanism of limb regeneration of <i>Hynobius yiwuensis</i>.","authors":"Hao Chen, Rui Dong, Yan-Feng Wang, Jing-Bei Xie, Zhao-Xu Zhang, Rong-Quan Zheng","doi":"10.16288/j.yczz.24-326","DOIUrl":"https://doi.org/10.16288/j.yczz.24-326","url":null,"abstract":"<p><p><i>Hynobius yiwuensis</i> is a vulnerable species endemic to China, restricted to specific hilly regions in Zhejiang Province. This study employed stereomicroscopy to examine the limb regeneration process in <i>H. yiwuensis</i> and utilized transcriptome sequencing to analyze differentially expressed genes. The results indicate that <i>H. yiwuensis</i> possesses strong regenerative capabilities, with the regeneration process comprising four stages: wound healing, tissue dissolution and dedifferentiation, blastema formation, and morphogenesis followed by redifferentiation. Transcriptome analysis identified numerous differentially expressed genes during limb regeneration, exhibiting distinct expression patterns at various time points post-amputation. Key differentially expressed genes were identified, including <i>IL10</i>, associated with cellular immunity and inflammation; <i>TGFβ3</i>, involved in early muscle tissue regeneration; and MMPs, implicated in tissue remodeling. qRT-PCR validation of selected differentially expressed genes confirmed the reliability of the transcriptome sequencing data. Preliminary findings suggest that <i>H. yiwuensis</i> regulates limb regeneration and promotes scar-free tissue repair through signaling pathways such as Wnt/β-catenin, TGFβ, and BMP.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"1032-1041"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid metabolism imbalance: potential pathological mechanism and new intervention ideas for recurrent miscarriage.","authors":"Wen-Hui Nan, Xun-Si Qin, Rong Li","doi":"10.16288/j.yczz.24-332","DOIUrl":"https://doi.org/10.16288/j.yczz.24-332","url":null,"abstract":"<p><p>Recurrent miscarriage is defined as three or more consecutive spontaneous abortions. Its etiology is complex, involving genetic, immune, and endocrine factors. In recent years, lipid metabolism disorders have attracted increasing attention for their potential role in recurrent miscarriage. Abnormalities in lipid metabolism can adversely affect endometrial receptivity, contributing to implantation failure. However, the precise underlying mechanisms remain to be fully elucidated. In this review, we provide an overview of the pathological mechanisms and recent advancements in research pertaining to the relationship between lipid metabolic disorders, embryonic development, and the establishment of endometrial receptivity. Special emphasis is placed on the influence of cholesterol and fatty acid metabolism on recurrent miscarriage. Furthermore, we examine the contributions of lipid metabolism to hormone synthesis and regulation, as well as the pathological changes that can arise from lipid-mediated local inflammation within the endometrium. Finally, we discuss the potential roles of lipid profile monitoring, dietary interventions, and lipid metabolism-targeted therapies in the early diagnosis and treatment of recurrent miscarriage. We hope this review can enhance understanding of the mechanisms underlying lipid metabolic dysfunction in this context and inform the development of tailored therapeutic strategies.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"979-991"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of <i>ppp6r3</i> in zebrafish gonadal differentiation and gametogenesis.","authors":"Yun-Hai Gao, Jia-Jie Deng, Xiao Xiao, Lu-Yuan Pan, Mu-Dan He, Yun-Bin Zhang","doi":"10.16288/j.yczz.25-093","DOIUrl":"https://doi.org/10.16288/j.yczz.25-093","url":null,"abstract":"<p><p>The gonad differentiation and gametogenesis of fish is regulated by various factors. Protein phosphatase (PP) have the function of catalyzing the dephosphorylation of proteins in organisms. As a member of the protein phosphatase family, protein phosphatase type 6 (PP6) plays an important role in gonadal development and gametogenesis. The role of <i>ppp6r3</i>, which encodes the regulatory subunit 3 of protein phosphatase 6, in gonadal differentiation and gametogenesis is still unclear. In this study, two zebrafish <i>ppp6r3</i> mutant lines were constructed using CRISPR/Cas9 technology. It was found that the absence of <i>ppp6r3</i> leads to the development of only male zebrafish, and these mutants are incapable of fertilizing wild-type eggs. Further investigations revealed that in the testes of <i>ppp6r3</i> mutants, the transition of spermatocytes to mature sperm was blocked, disrupting spermatogenesis. In summary, this study established a <i>ppp6r3</i> mutant model, exhibiting defects in gonadal differentiation and gametogenesis, which provides a new model for further research on the mechanisms by which Ppp6r3 regulates germ cell proliferation, differentiation, and sex determination.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"1023-1031"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of the composition and distribution of anti-phage defense systems in <i>Vibrio</i> genomes.","authors":"Xue-Feng Xu, Xing-Kun Jin, Yan Shi, Zhe Zhao","doi":"10.16288/j.yczz.24-290","DOIUrl":"https://doi.org/10.16288/j.yczz.24-290","url":null,"abstract":"<p><p>The long-term co-evolution between bacteria and their viruses (bacteriophages) drives the diversification of anti-phage defense systems. As key members of marine ecosystems, <i>Vibrio</i> species are known for their large and complex genomes. To investigate the composition and distribution of anti-phage defense systems in <i>Vibrio</i> genomes, we collect 242 representative genomes from the GTDB and NCBI databases. Using bioinformatics tools (including Defense Finder), we analyze the anti-phage defense systems encoded by these genomes and identify a total of 108 distinct systems, including GAPS, dGTPase, RM systems, etc. We observe significant variation in defense capabilities among different <i>Vibrio</i> strains: five strains encode more than 20 defense systems, highlighting their high defense potential, while over 30 strains encode five or fewer defense systems. Notably, we find no known defense systems in '<i>Vibrio katoptron</i>'. Furthermore, we note that the diversity of defense systems varies among <i>Vibrio</i> clades, with the Cholerae clade exhibiting the highest entropy in its defense systems. We conduct a detailed analysis of the composition, structure, and functional mechanisms of these defense systems. Although <i>Vibrio</i> species exhibit a complex and diverse array of anti-phage defense systems, the specific functions of many remain unknown. Given the complexity of <i>Vibrio</i> genomes, we suggest that numerous potential defense systems are yet to be discovered. This study provides a comprehensive overview of the genomic characteristics of <i>Vibrio</i> defense systems, laying a foundation for future research into the interactions and evolutionary dynamics between <i>Vibrio</i> and bacteriophages.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"1057-1068"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AARS1/2: dual functions in protein translation and metabolic- immune regulation.","authors":"Zong-Wang Zhang, Jing-Wei Xiong","doi":"10.16288/j.yczz.25-034","DOIUrl":"https://doi.org/10.16288/j.yczz.25-034","url":null,"abstract":"<p><p>Highly precise regulation of protein synthesis is critical for the homeostasis and functionality of living organisms. Alanyl-tRNA synthase (AARS1/2) plays a crucial role in this process. AARS1/2 are a class of enzymes that synthesize alanyl-tRNA in cells, participating in protein synthesis encoded by genes, and catalyzing the propionylation of lysine residues in proteins, thereby regulating protein function. This article reviews the research progress on the involvement of AARS1/2 in disease progression induced by protein mistranslation and in the regulation of the metabolic-immune interaction network, aiming to better understand the pathophysiological mechanisms of AARS1/2 and to provide a reference for the development of potential therapeutic drugs.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 9","pages":"967-978"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on small non-coding RNAs in male reproductive development and intergenerational inheritance.","authors":"Yu-Qian Shi, Jian-Feng Ma, Si-Yu Chen, Li-Xin Zhou, Jia Xue, Lin-Yuan Shen, Li Zhu, Mai-Lin Gan","doi":"10.16288/j.yczz.24-335","DOIUrl":"10.16288/j.yczz.24-335","url":null,"abstract":"<p><p>Small non-coding RNAs (sncRNAs) are crucial in epigenetics, playing a significant regulatory role in the normal development and intergenerational inheritance of male reproduction. Research has shown that highly expressed sncRNAs, including miRNAs, piRNAs, and tsRNAs, are vital in maintaining male germ cell development and spermatogenesis. sncRNAs regulate gene expression, influence protein translation, and modify sperm epigenetics, contributing to male reproductive development at various stages. Abnormal expression of sncRNAs is closely linked to male infertility. Furthermore, growing evidence suggests that environmental exposures affect sperm epigenetic modifications, often leading to phenotypic changes in future generations. In this review, we summarize the types and functions of sncRNAs in male germ cells and examine their role in intergenerational inheritance due to environmental factors. It aims to provide new insights into male reproductive health and potential targets for preventing and treating male infertility and related diseases.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"861-875"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry-based analysis of RNA and its modifications.","authors":"Ying Feng, Xiao-Li He, Yu Liu, Jin Wang","doi":"10.16288/j.yczz.25-052","DOIUrl":"10.16288/j.yczz.25-052","url":null,"abstract":"<p><p>Ribonucleic acids (RNAs) are key biomolecules responsible for the transmission of genetic information, the synthesis of proteins and its regulation, and modulation of many biochemical processes. They are also the key components of many viruses. Chemically modified synthetic RNAs or oligoribonucleotides are becoming more widely used as therapeutics and vaccines. Demands for technologies to detect, sequence, identify, and quantify RNA and its modifications far exceed requirements found in the DNA realm. Currently, mass spectrometry (MS) has become the primary technology for identifying, sequencing, and quantifying RNA and its modifications. This paper mainly reviews latest advances in mass spectrometry for the research of RNA and its modifications, and discusses the strengths and weaknesses of this technology, aiming to provide readers with a comprehensive perspective from technical fundamentals to application prospects, promote the broader application of mass spectrometry in RNA research, and provide important references for method developers and biological researchers in the field.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"885-902"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}