遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-283
Hao-Yu Luo, Xiao-Hong Yan, Fang Mu, Ning Wang
{"title":"Recent advances in the study of 3' untranslated regions of eukaryotic mRNAs.","authors":"Hao-Yu Luo, Xiao-Hong Yan, Fang Mu, Ning Wang","doi":"10.16288/j.yczz.24-283","DOIUrl":"https://doi.org/10.16288/j.yczz.24-283","url":null,"abstract":"<p><p>The 3' untranslated regions (3'UTRs) are a component of eukaryotic messenger RNAs (mRNAs) and located at their 3'-most ends. 3'UTRs play crucial roles in post-transcriptional regulation of gene expression, regulating cleavage and polyadenylation, mRNA stability, mRNA translation and localization. With deepening research of the 3'UTRs, and with advent of various omics methods, a few unexpected functions of the eukaryotic 3'UTRs have been uncovered. In this review, we summarize recent advances in the study of 3'UTRs and propose the future research directions for studying the roles and mechanisms of the 3'UTRs, with the aim to provide a better reference for comprehensively investigating gene function and regulation.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"616-624"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Harmonizing safety and development: effective conservation and sustainable utilization of China's human genetic resources.","authors":"Min Zhu, Huan-Lu Tan, Tian-Yu Zhao, Lin-Xiao Wang, Miao Kuang, Jian-Zhong Shen","doi":"10.16288/j.yczz.25-049","DOIUrl":"10.16288/j.yczz.25-049","url":null,"abstract":"<p><p>The management of human genetic resources constitutes a critical part of the national biosafety system. Its importance increasingly underscored by rapid development in biotechnology. After years of efforts, China has established a relatively comprehensive management system in areas such as legal infrastructure, regulatory mechanisms, and the conservation and utilization of human genetic resources. Nevertheless, facing the new demands of industry development in the new era and new situation, China still encounters numerous problems in the management of human genetic resources, such as less effective protection, inadequate rational utilization, a shortage of specialized personnel, and insufficient regulatory capabilities. To address these problems, this article systematically reviews the development of China's human genetic resources management system, analyzes the current main risks and challenges, and proposes targeted policy recommendations to harmonize development with safety imperatives, thereby facilitating the effective conservation and sustainable utilization of human genetic resources in China.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"611-615"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-282
Yi-Min Zhao, Rui-Xuan Li, Zi-Yi Qin, Yong Wang
{"title":"Progress on the role of eicosanoids in insect immunity.","authors":"Yi-Min Zhao, Rui-Xuan Li, Zi-Yi Qin, Yong Wang","doi":"10.16288/j.yczz.24-282","DOIUrl":"https://doi.org/10.16288/j.yczz.24-282","url":null,"abstract":"<p><p>Eicosanoids are synthesized from arachidonic acid, which are oxygenated metabolites of C20 polyunsaturated fatty acids through cyclooxygenases (COXs) or lipoxygenases (LOXs). Eicosanoids include prostaglandins (PGs), thromboxanes (TXs), epoxyeicosatrienoic acids (EETs) and leukotrienes (LTs), etc. Eicosanoids mediate a number of physiological processes in insects, such as Malpighian tubule and ovary development, or immune response. They play the important roles in regulating cellular immune processes to clear bacteria, fungi, parasites and viruses by hemocytes and the humoral immune pathways. In this review, we summarize the pathways of eicosanoids synthesis, different types of insect immune response and relationship between eicosanoids and humoral immune system. Understanding the role of eicosanoids in insect immune system, and key enzymes or inhibitors in the pathway of eicosanoids synthesis, will provide theoretical guide for the research in pathogen and insects interaction.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"636-649"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-307
Xin-Ru Xu, Ben-Hua Qiu, Xiu-Min Yan
{"title":"Application and progress of proximity labeling technology in the study of protein-protein interactions.","authors":"Xin-Ru Xu, Ben-Hua Qiu, Xiu-Min Yan","doi":"10.16288/j.yczz.24-307","DOIUrl":"https://doi.org/10.16288/j.yczz.24-307","url":null,"abstract":"<p><p>Protein-protein interactions are essential to almost all biological processes within cells. In recent years, proximity labeling technology has emerged as a cutting-edge approach for studying protein interactions and subcellular compartment proteomics. This technique enables labeling and capture of neighboring proteins by introducing chemical tags in the vicinity of a target protein. When combined with mass spectrometry, proximity labeling facilitates the effective identification of protein-protein interactions and proteomic landscapes of subcellular compartments with high spatiotemporal resolution. Compared to traditional methods, proximity labeling offers superior resolution, sensitivity, and real-time tracking capabilities,making it a powerful tool for investigating protein functions, interactions, and spatial proteomics within cells. In this review, we provide a comprehensive overview of the principles, classifications, and applications of proximity labeling technology, aiming to offer researchers valuable insights into this innovative methodology and to promote its broader application in cell biology, proteomics, and the study of disease mechanisms.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"625-635"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-329
Nian Guo, Chen-Jie Wang, Zhao Li, Nan-Nan Han, Chen Zhou, Kai-Ying Wang, Ke Huang, Yong-Qing Pan, Ying-Jie Li, Yun-Hai Li
{"title":"Genetic analysis and identification of candidate genes for a narrow and long grain mutant (<i>nlg1</i>) in rice.","authors":"Nian Guo, Chen-Jie Wang, Zhao Li, Nan-Nan Han, Chen Zhou, Kai-Ying Wang, Ke Huang, Yong-Qing Pan, Ying-Jie Li, Yun-Hai Li","doi":"10.16288/j.yczz.24-329","DOIUrl":"https://doi.org/10.16288/j.yczz.24-329","url":null,"abstract":"<p><p>Grain size is one of the components that determine the rice yield. Exploring more key genes that regulating grain size and analyzing their molecular mechanisms are of great importance for precision breeding of rice. In this study, we screened a mutant <i>nlg1</i>(narrow and long <u>g</u>rain 1) with narrow and long grains by mutagenizing the japonica rice variety ZH11 with EMS. Observation and statistical analysis of the outer surface of the mature glume of <i>nlg1</i> by scanning electron microscopy revealed an increased number of longitudinal cells and a decreased number of transverse cells, along with a reduction in the width of transverse cells. These findings suggest that <i>NLG1</i> may regulate grain size by simultaneously influencing the proliferation and expansion of lemma cells. The candidate gene was cloned by genome resequencing combined with MutMap analysis method. The results showed that the candidate gene of LOC_Os09g27590 was the previous reported gene, <i>GS9</i>, which encodes a protein with unknown domains. In the <i>nlg1</i> mutant, a base C was inserted in the first exon of the candidate gene of LOC_Os09g27590, causing a frameshift mutation that leads to premature termination of protein translation. Further genetic complementation experiments showed that <i>NLG1</i> rescued the phenotype of the <i>nlg1</i> mutant. Therefore, this study identifies a novel <i>GS9</i> allele mutation, and further dissection of its molecular regulatory networks in grain size control can provides a theoretical support and genetic resources for precise rice breeding.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"672-680"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-302
Hui-Xin Yu, Xian-Peng Zhang, Lan-Hai Wei
{"title":"The 30-100 patrilineal nomenclature system for eastern Eurasian populations.","authors":"Hui-Xin Yu, Xian-Peng Zhang, Lan-Hai Wei","doi":"10.16288/j.yczz.24-302","DOIUrl":"https://doi.org/10.16288/j.yczz.24-302","url":null,"abstract":"<p><p>Due to the universality of human patrilineal societies, strict adherence to the patrilineal inheritance law, and a relatively fast mutation rate (about one new branch every 70 years), the non-recombining region of the human Y-chromosome (NRY) can rapidly accumulate specific patrilineal branches that are specific to certain region, population, family, and family branch, making the Y-chromosome one of the powerful tools for studying the evolutionary history of human populations. However, early studies typically only included a limited number of SNP loci, and the classification systems in different articles varied, resulting in insufficient resolution for the evolutionary process of populations. Since 2015, with the rapid accumulation of whole-genome sequence data, the resolution of the human Y chromosome phylogenetic tree at the population level has approached its limit. In this study, we analyze the relevant research on the human Y chromosome in the past 30 years. Based on the currently highly detailed data, we identify 50 major patrilineal types or founder patrilineal types that are directly related to the origin and evolution of 16 population groups in eastern Eurasia. Furthermore, we construct a 30-100 classification system that is compatible with the diverse traditional classification systems in the past 30 years and the current most detailed phylogenies. This system takes into account both the macroscopic genetic structure of eastern Eurasian populations over a timescale of tens of thousands of years and the specific lineages directly related to the several-thousand-year history of modern ethnic groups, laying the foundation for more precise studies of the evolutionary process of ancient and modern populations in the future.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"660-671"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-209
Yao Shen, Zhi-Yu Li, Feng-Cheng Miao, Ying-Ping Xiao, Hua Yang, Ya-Li Dang, Jian-Gang Ma
{"title":"Progresses on the prevalence and mechanism of vancomycin- resistant bacteria.","authors":"Yao Shen, Zhi-Yu Li, Feng-Cheng Miao, Ying-Ping Xiao, Hua Yang, Ya-Li Dang, Jian-Gang Ma","doi":"10.16288/j.yczz.24-209","DOIUrl":"https://doi.org/10.16288/j.yczz.24-209","url":null,"abstract":"<p><p>Vancomycin, a glycopeptide antibiotic, serves as the last-resort treatment for infections caused by methicillin- resistant <i>Staphylococcus aureus</i> (MRSA), vancomycin-resistant <i>Enterococci</i> (VRE), and Clostridium difficile. However, the emergence of various vancomycin-resistant bacterial strains worldwide poses a significant challenge to clinical therapy. Adopting the \"One Health\" concept, we mainly present the prevalence of vancomycin-resistant bacteria over the past decade from 40 human, animal, environmental, and food sources across various regions, both domestically and internationally. The statistical results indicate that vancomycin-resistant bacteria are primarily concentrated in hospitals and their surrounding environments. The prevalence of resistant bacteria in hospital wastewater in South Africa reaches as high as 96.77%, followed by Pakistan and China's Taiwan region, where the resistance rates are 56.5% and 29.02%, respectively. The vancomycin average resistance rate in domestic human-source bacteria (1.41%) is overall higher than that in international human-source bacteria (0.47%). The prevalence of resistant bacteria in pediatric patients across various regions is relatively low (<1%). It is worth noting that although the use of vancomycin is prohibited in livestock farming, vancomycin- resistant bacteria can still be detected in livestock, related products and environment, posing a potential threat to human health. Based on the statistical analysis results, we summarize several common vancomycin resistance mechanisms and the transmission mechanisms, and clarify the differences in the prevalence of resistant bacteria across the \"human-animal-food-environment\" interface for further analyzing the distribution and transmission risks of vancomycin-resistant bacteria in different hosts worldwide. This review can also provide references for the prevention and control of antimicrobial resistance.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"650-659"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on population genomics of <i>Bacillus anthracis</i> based on multiple types of genetic variations.","authors":"Zu-Ming Zhang, Hao Zhou, Xue-Zhi Huang, Duo-Yue Zhang, Jia-Yi Zhang, Yu Lin, Li-Wei Fang, Xiu-Chang Zhang, Yu-Jun Cui, Ya-Rong Wu, Yan-Jun Li","doi":"10.16288/j.yczz.24-295","DOIUrl":"https://doi.org/10.16288/j.yczz.24-295","url":null,"abstract":"<p><p><i>Bacillus anthracis</i>, the causative agent of the deadly infectious disease anthrax, is also a typical biological warfare agent. It primarily infects livestock such as cattle and sheep, as well as humans, causing significant economic losses to the livestock industry and posing a threat to human society. A more profound insight into the genetic diversity and evolutionary drivers of this species is essential for studying its virulence mechanisms and conducting anthrax surveillance and control. However, current research in this area is insufficient, particularly lacking population genomic studies based on multiple types of genetic variation. In this study, we collect and filter the genome sequences of 1,628 publicly available <i>B. anthracis</i> strains, and identify various types of variation in 1,347 high-quality sequences, including SNPs, indels, large fragment gains and losses, copy number variations (CNVs), and genome rearrangements. In total, we identify 26,635 SNPs, 9,997 indels, 21 large fragment gains and losses, 25 CNVs, and 5 inversions. Phylogenetic reconstruction reveals that this species can be divided into six major populations and 17 subgroups. By integrating population diversity and geographic distribution characteristics, we find that U.S. strains exhibit the highest genetic diversity, while African strains show significant geographic clustering. Additionally, through selection pressure analysis, we identify strong selection signals in four genes (<i>rpoB, fusA, spo0F</i>, <i>GBAA</i>_<i>RS11385</i>) related to drug resistance and sporulation. This study reconstructs the global population structure of <i>B. anthracis</i> and reveals key variations during the species' evolutionary process, providing targets for anthrax strain identification, tracing, and virulence mechanism research, as well as scientific support for the prevention and control of anthrax outbreaks.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"681-693"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-06-01DOI: 10.16288/j.yczz.24-322
Jia-Tong Yan, Guan-Wei Chen, Qing-Mei Miao, Cheng Peng, Lei Yang, Xiao-Yun Chen, Xiao-Li Xu, Wei Wei, Jun-Feng Xu, Xiao-Fu Wang
{"title":"Onsite rapid detection method for genetically modified maize and soybean based on recombinase polymerase amplification.","authors":"Jia-Tong Yan, Guan-Wei Chen, Qing-Mei Miao, Cheng Peng, Lei Yang, Xiao-Yun Chen, Xiao-Li Xu, Wei Wei, Jun-Feng Xu, Xiao-Fu Wang","doi":"10.16288/j.yczz.24-322","DOIUrl":"https://doi.org/10.16288/j.yczz.24-322","url":null,"abstract":"<p><p>With the commercial planting of transgenic maize and transgenic soybeans in China, in order to ensure the healthy development of China's biological breeding industry, it is urgent to develop on-site rapid detection techniques for transgenic maize and soybeans. In this study, a rapid detection method based on recombinase polymerase amplification (RPA) was proposed to overcome the shortcomings of on-site rapid detection of transgenic maize and soybean. Representative transgenic maize (Shuang Kang 12-5, DBN9936, MON810) and soybean (DBN9004, GTS40-3-2, SHZD32-1) were selected as test objects. According to their flanking sequence, specific primers and probes were designed and screened, and a RPA amplification system with high specificity and sensitivity of 20 copies for each transgenic event was established. In order to quickly obtain DNA, seed samples use nucleic acid release agents to obtain templates that can be used for RPA amplification. In order to eliminate the interference of chlorophyll in leaves on RPA fluorescence signal and quickly obtain DNA, leaf samples use the nucleic acid rapid integrated extraction device developed by our laboratory to quickly obtain DNA and eliminate the chlorophyll interference. Combined with a portable metal bath for initiating RPA amplification, a blue light gel cutter for visual detection, and a rapid on-site detection method for transgenic maize and soybean was constructed. The entire detection process required approximately 20 minutes, and the detection result was consistent with the result of conventional PCR. It is suitable for on-site rapid detection without large equipment, and has strong practicability and adaptability. The proposed approach provides technical support for on-site detection of transgenic crops and offers a reference for the application of other rapid nucleic acid detection technologies.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"694-707"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research advances in rice blast resistance genes.","authors":"Zhan-Chun Wang, Gui-Tao Zhong, Bei-Bei Zhang, Yi-Lin Xie, Ding-Zhong Tang, Wei Wang","doi":"10.16288/j.yczz.24-358","DOIUrl":"https://doi.org/10.16288/j.yczz.24-358","url":null,"abstract":"<p><p>Rice (<i>Oryza sativa</i> L.) is an important food crop in China. Its growth and development are threatened by rice blast, which leads to huge grain losses in severe cases. Cultivating and deploying rice varieties that contain broad-spectrum resistance (<i>R</i>) genes is the most economical and effective strategy to prevent and control rice blast. In general, rice blast resistance genes code nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) that play a crucial role in the resistance to rice blast. Therefore, it is essential to clone <i>NLR</i> genes and elucidate the recognition and activation mechanisms between NLRs and effectors for rice disease resistance breeding. In this review, we summarize and provide perspectives on the research progress of <i>NLR</i> genes, the recognition between NLR proteins and effectors in rice, as well as the role and mechanism of paired NLR proteins in the resistance, hoping to provide reference for rice disease resistance breeding.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"533-545"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}