{"title":"Mass spectrometry-based analysis of RNA and its modifications.","authors":"Ying Feng, Xiao-Li He, Yu Liu, Jin Wang","doi":"10.16288/j.yczz.25-052","DOIUrl":null,"url":null,"abstract":"<p><p>Ribonucleic acids (RNAs) are key biomolecules responsible for the transmission of genetic information, the synthesis of proteins and its regulation, and modulation of many biochemical processes. They are also the key components of many viruses. Chemically modified synthetic RNAs or oligoribonucleotides are becoming more widely used as therapeutics and vaccines. Demands for technologies to detect, sequence, identify, and quantify RNA and its modifications far exceed requirements found in the DNA realm. Currently, mass spectrometry (MS) has become the primary technology for identifying, sequencing, and quantifying RNA and its modifications. This paper mainly reviews latest advances in mass spectrometry for the research of RNA and its modifications, and discusses the strengths and weaknesses of this technology, aiming to provide readers with a comprehensive perspective from technical fundamentals to application prospects, promote the broader application of mass spectrometry in RNA research, and provide important references for method developers and biological researchers in the field.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 8","pages":"885-902"},"PeriodicalIF":0.0000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"遗传","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.16288/j.yczz.25-052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
Ribonucleic acids (RNAs) are key biomolecules responsible for the transmission of genetic information, the synthesis of proteins and its regulation, and modulation of many biochemical processes. They are also the key components of many viruses. Chemically modified synthetic RNAs or oligoribonucleotides are becoming more widely used as therapeutics and vaccines. Demands for technologies to detect, sequence, identify, and quantify RNA and its modifications far exceed requirements found in the DNA realm. Currently, mass spectrometry (MS) has become the primary technology for identifying, sequencing, and quantifying RNA and its modifications. This paper mainly reviews latest advances in mass spectrometry for the research of RNA and its modifications, and discusses the strengths and weaknesses of this technology, aiming to provide readers with a comprehensive perspective from technical fundamentals to application prospects, promote the broader application of mass spectrometry in RNA research, and provide important references for method developers and biological researchers in the field.
期刊介绍：
Hereditas is a national academic journal sponsored by the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences and the Chinese Society of Genetics and published by Science Press. It is a Chinese core journal and a Chinese high-quality scientific journal. The journal mainly publishes innovative research papers in the fields of genetics, genomics, cell biology, developmental biology, biological evolution, genetic engineering and biotechnology; new technologies and new methods; monographs and reviews on hot issues in the discipline; academic debates and discussions; experience in genetics teaching; introductions to famous geneticists at home and abroad; genetic counseling; information on academic conferences at home and abroad, etc. Main columns: review, frontier focus, research report, technology and method, resources and platform, experimental operation guide, genetic resources, genetics teaching, scientific news, etc.
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