针刺研究最新文献

{"title":"Electroacupuncture promotes angiogenesis by regulating miR-142-5p and activating ADAMTS1/PI3K/AKT pathway in ischemic stroke rats.","authors":"Shu-Han Si, Shi-Zhen Song, Xue-Yun Xie, Ting-Ting Zhang, Xiao-Fei Kui, Xiao-Hui Yang, Shuang Zhou","doi":"10.13702/j.1000-0607.20230323","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230323","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture on miR-142-5p and ADAMTS1/PI3K/AKT pathway in rats with ischemic stroke, so as to explore the regulatory mechanism of electroacupuncture on angiogenesis after ischemic stroke.</p><p><strong>Methods: </strong>This study was divided into two parts. The first part of the experiment：SD rats were randomly divided into sham operation group, model group and electroacupuncture group. There were 20 rats in each group. The middle cerebral artery occlusion (MCAO) rat model was prepared using a modified Longa's method. In the electroacupuncture group, \"Shuigou\" (GV26) was selected for electroacupuncture intervention (4 Hz/20 Hz) for 30 min each time. The rats in the electroacupuncture group were given electroacupuncture immediately after successful modeling, once a day for 4 times. Hunter score and TTC staining were used to observe the neurological deficits and infarct volumes respectively；HE staining was used to observe the cortical pathological changes；immunohistochemistry was used to determine the changes of cerebral microvascular density. Real-time quantitative PCR and Western blot were used to observe the miR-142-5p expression, mRNA and protein expression levels of ADAMTS1, VEGF, PI3K, AKT, eNOS in ischemic cortex. The second part of the experiment：The rats were randomly divided into electroacupuncture+control group and electroacupuncture+miR-142-5p Antagomir group with 8 rats in each group. MCAO model was established after injection. Electroacupuncture+control group was given 0.9% sodium chloride solution injected into the right ventricle.The rats in the electroacupuncture+miR-142-5p Antagomir group were injected with miR-142-5p inhibitor into the right ventricle 30 min before modeling. Rats in electroacupuncture+control group and electroacupuncture+miR-142-5p Antagomir group were all given the same electroacupuncture treatment. Real-time fluorescence quantitative PCR was used to observe the effect of miR-142-5p Antagomir on the expression of miR-142-5p and ADAMTS1 mRNA. The effect of miR-142-5p Antagomir on ADAMTS1 protein was observed by Western blot.</p><p><strong>Results: </strong>In the first part of the experiment, compared with the sham operation group, the Hunter score in the model group was significantly increased (<i>P</i><0.01)；the volume of cerebral infarction in the model group was significantly increased (<i>P</i><0.01)；the degree of brain edema and neuronal necrosis and the density of cerebral microvessels was increased；the cerebral microvascular density was significantly increased (<i>P</i><0.01)；the expression levels of miR-142-5p and the mRNA expression levels of VEGF, AKT and eNOS were significantly decreased (<i>P</i><0.01, <i>P</i><0.05), and the protein expression levels of VEGF, p-AKT and eNOS were significantly down-regulated (<i>P</i><0.01), while the mRNA expression levels of ADAMTS1 and PI3K, and the protein expression levels of ADAMTS1 and p-PI3K wer","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"787-796"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moxibustion with seed-size moxa cones alleviates colonic injury in mice with ulcerative colitis by regulating TLR4/MyD88/NF-κB signaling pathway.","authors":"Tao Zhu, Lai-Xi Ji, Jia Ren, Yan-Ting Cheng, Yu-Fang Ji, Zhen Gao, Jing Zhang, Hai-Jun Wang","doi":"10.13702/j.1000-0607.20240189","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240189","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of moxibustion with seed-size moxa cones on the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear transcription factor-κB(NF-κB) signaling pathway in mice with ulcerative colitis(UC), so as to explore the therapeutic mechanism of moxibustion with seed-size moxa cones on colonic injury in UC.</p><p><strong>Methods: </strong>Forty male C57BL/6 mice were randomly divided into blank group, model group, moxibustion group, and western medicine group, with 10 mice in each group. The UC mouse model was established by 3% DSS solution by free drinking for 7 consecutive days. Mice in the moxibustion group were treated with seed-size moxa cones at \"Zhongwan\"(CV12), \"Tianshu\"(ST25) and \"Shangjuxu\"(ST37), 3 moxa cones per point, with each cone applied for approximately 30 s, while mice in the western medicine group were orally administered with 300 mg/kg mesalazine solution, which were both conducted once a day for 7 consecutive days. The general condition of mice was observed every 2 days, and the disease activity index (DAI) score was calculated. HE staining was used to observe the morphology of colonic tissue in mice. ELISA was used to detect the serum interleukin(IL)-1β, tumor necrosis factor(TNF)-α, IL-6, and IL-8 contents. Immunohistochemistry was used to detect the positive expression of TLR4 and MyD88 in colonic tissue of mice. Real-time fluorescence quantitative PCR was used to detect the expression levels of TLR4, MyD88, and NF-κB p65 mRNAs in colonic tissue.</p><p><strong>Results: </strong>Compared with the blank group, varying degrees of soft or watery stools were observed, colon length and body weight were decreased(<i>P</i><0.01) in mice of the model group, while DAI score, colon weight index, mucosal damage score, colonic pathological score, serum IL-1β, TNF-α, IL-6, and IL-8 contents, positive expressions of TLR4 and MyD88, and TLR4, MyD88, and NF-κB p65 mRNA expressions in colonic tissue were increased(<i>P</i><0.01). Compared with the model group, improved fecal characteristics were observed, colon length and body weight were increased(<i>P</i><0.01) in mice of the moxibustion group and western medicine group, while DAI scores, colon weight indexes, mucosal damage scores, colonic pathological score, serum contents of IL-1β, TNF-α, IL-6, and IL-8, positive expressions of TLR4 and MyD88, and TLR4, MyD88, and NF-κB p65 mRNA expressions in colonic tissue were decreased(<i>P</i><0.01, <i>P</i><0.05). There was no significant difference in the above indicators between the moxibustion group and the western medicine group.</p><p><strong>Conclusions: </strong>Moxibustion with seed-size moxa cones may alleviate colonic injury in UC mice by regulating the TLR4/MyD88/NF-κB signaling pathway and reducing the release of inflammatory factors.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"836-844"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture alleviates neuropathic pain by regulating hippocampal SP/NK1R/ARRB1 pathway.","authors":"Jia-Yi Liang, Feng-Jiao Wang, Gong-Ming Wang, Yi-Ming Gu, Yong Xia, Ke Wang, Zi-Yong Ju","doi":"10.13702/j.1000-0607.20230520","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230520","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) on pain, anxiety like behavior, and substance P(SP) /neurokinin-1 receptor (NK1R) /β -arrestin 1(ARRB1) pathway related protein expression in hippocampus of chronic constriction injury (CCI) rats, so as to explore its mechanisms underlying improvement of neuropathic pain.</p><p><strong>Methods: </strong>Twenty-seven male SD rats were randomly divided into sham operation, model and EA groups, with 9 rats in each group. The CCI model was established by ligature of the left sciatic nerve. On the 8^th day following modeling, EA (2 Hz, 0.5-1.5 mA) was applied to the left \"Huantiao\" (GB34) and \"Yanglingquan\" (GB34) for 30 min, once every other day for 13 times. Mechanical paw withdrawal threshold (MWT), thermal paw withdrawal threshold (TWL) and difference of the weight distribution of the hind limbs were detected before operation and at the 5^th, 9^th, 17^th, 25^th and 33^rd days after operation. Open field test was used to evaluate the anxiety-like behavior of rats. The content of SP in hippocampus was determined by ELISA. The protein expression of NK1R and ARRB1 in hippocampus was detected by Western blot.</p><p><strong>Results: </strong>Compared with the sham operation group, the MWT and TWL of the left hind limb at the 5^th, 9^th, 17^th, 25^th and 33^rd days after operation, the time of entering the central area and the total distance of movement, and the content of SP in the hippocampus were significantly decreased (<i>P</i><0.001, <i>P</i><0.01), while the difference of the weight distribution of the hind limbs at the 5^th, 9^th, 17^th, 25^th and 33^rd days after operation and the protein expression of NK1R and ARRB1 were significantly increased (<i>P</i><0.001, <i>P</i><0.05) in the model group. After EA intervention, the MWT and TWL of the left hind limb, the time of entering the central area and the total moving distance, and the expression of SP in the hippocampus were significantly increased (<i>P</i><0.01, <i>P</i><0.001, <i>P</i><0.05), while the difference in the weight distribution of the hind limbs was significantly reduced, and the expression of NK1R and ARRB1 protein in the hippocampus were significantly decreased (<i>P</i><0.001, <i>P</i><0.05) in the EA group.</p><p><strong>Conclusions: </strong>EA can effectively improve the pain and anxiety behaviors in CCI rats, and reverse the abnormal expression of SP, NK1R and ARRB1 proteins in the hippocampus, which may be related to its effects in regulating the SP/NK1R/ARRB1 pathway in the hippocampus.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"829-835"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of researches on the mechanisms of acupuncture intervention underlying improvement of ischemic stroke by regulating microRNAs.","authors":"Cheng-Long Li, Wei Mao, Li-da Zhang, Wei Han, Xin Zhang, Ying Wang","doi":"10.13702/j.1000-0607.20230031","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230031","url":null,"abstract":"<p><p>MicroRNAs play an important role in the occurrence and development of ischemic stroke (IS). A lot of researches have shown that acupuncture intervention can improve IS-induced neural dysfunction by regulating miRNA. In the present paper, we summarized the current progress of researches on the mechanisms of acupuncture underlying improvement of IS via regulation of miRNA from 1) promoting angiogenesis and increasing cerebral blood flow, 2) inhibiting inflammatory response, 3) maintaining blood-brain barrier homeostasis and relieving brain edema, 4) regulating programmed cell death, 5) promoting neuron regeneration, and 6) improving synaptic plasticity. These miRNA -related mechanisms may provide a reference for the follow-up research .</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"851-857"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of moxibustion on ATP-sensitive potassium channel (K_ATP) of bladder in detrusor overactivity rats.","authors":"Yu-Xiang Liao, Lu Liu, Yuan-Bo Fu, Peng Chen, Tao Zhang, Fan Zhang, Ya-Li Wen, Shao-Song Wang, Xin DU, Jun Yang, Jiang-Yan Wei, Qi An, Shu-Han Jia, Hui-Lin Liu, Bin Li","doi":"10.13702/j.1000-0607.20230086","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230086","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of ginger-salt-partitioned moxibustion on ATP-sensitive potassium (K_ATP) channel of bladder in detrusor overactivity (DO) rats.</p><p><strong>Methods: </strong>Female SD rats were randomly divided into sham operation, model, moxibustion and antagonist groups (<i>n</i>=9 in each group). Thorax (T) 10 spinal cord transection was performed by surgery. Ginger-salt partitioned moxibustion was applied to \"Shenque\" (CV8) for 3 cones, once daily for 14 consecutive days. Rats of the antagonist group were intraperitoneally injected with K_ATP channel specific antagonist glibenclamide (10 μg·kg^-1·d^-1) once daily for 14 consecutive days. Urodynamic tests were performed after treatment. The distribution and expression of K_ATP channel tetrameric subunit (SUR2B) in the bladder of rats was observed by immunofluorescence. The protein and mRNA expression levels of SUR2B in bladder tissue were detected by Western blot and qPCR respectively.</p><p><strong>Results: </strong>Compared with the sham operation group, rats of the model group showed intensive and large phasic contractions of the detrusor during bladder filling, the frequency and amplitude of phasic contractions of the detrusor 5 min before leakage were significantly increased (<i>P</i><0.001)；the voiding threshold pressure was significantly decreased (<i>P</i><0.001)；the bladder perfusion volume was increased (<i>P</i><0.001)；the SUR2B protein and mRNA expression in bladder tissue were significantly reduced (<i>P</i><0.001). Compared with the model group and the antagonist group, the above-mentioned indicators in the moxibustion group were all reversed (<i>P</i><0.01, <i>P</i><0.001, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Ginger-salt partitioned moxibustion can reduce the frequency and amplitude of detrusor phase contraction during bladder filling and prolong the time of first phase contraction in DO rats, which may be associated with up-regulating the expression level of K_ATP channel protein and mRNA, promoting the outflow of potassium ions, and inhibiting the inflow of calcium ions, thus improve the stability of detrusor during storage.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"814-820"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of electroacupuncture on expression of autophagy-related proteins in nucleus pulposus of cervical spondylosis rabbits.","authors":"Jia-Li Chen, Zi-Ying Zhao, Hang Gao, Yin-Qing Xu, Guang-Yi Wang","doi":"10.13702/j.1000-0607.20230488","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230488","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effects of electroacupuncture (EA) on the morphological changes of intervertebral disc tissues, apoptosis of nucleus pulposus cells, and the protein expression of Unc-51 like autophagy-activated kinase 1 (ULK1), homologous series of yeast Atg6 (Beclin1), and light chain protease complication 3 type (LC3) in nucleus pulposus tissue of cervical spondylosis rabbits, so as to explore the role of cellular autophagy in EA treatment of cervical spondylosis.</p><p><strong>Methods: </strong>A total of 24 New Zealand white rabbits were randomly divided into blank, model and EA groups, with 8 rabbits in each group. In the EA group, both sides of the cervical (C)3-C6 \"Jiaji\" (EX-B2) were stimulated by EA (2 Hz/100 Hz, 1 mA) for 25 min, once daily for 5 days in a course, with a 2-day interval between courses, totaling 4 treatment courses. X-ray was used to assess cervical spine radiographic changes and evaluate radiographic scores；transmission electron microscopy was used to observe ultrastructural changes in nucleus pulposus cells；HE staining was used to observe morphological changes of intervertebral disc tissues and conduct pathological scoring；TUNEL staining was used to observe apoptosis rate of nucleus pulposus cells；Western blot was performed to detect protein expression levels of ULK1, Beclin1, and LC3 in nucleus pulposus tissue.</p><p><strong>Results: </strong>Compared with the blank group, rabbits in the model group showed significantly higher cervical spine radiographic scores (<i>P</i><0.01), higher pathological scores of intervertebral disc tissues (<i>P</i><0.05), increased apoptosis rate of nucleus pulposus cells (<i>P</i><0.01), and decreased expression levels of ULK1, Beclin1, and LC3Ⅱ proteins in nucleus pulposus tissue (<i>P</i><0.05). Compared with the model group, the EA group showed significantly lower pathological scores of intervertebral discs (<i>P</i><0.05), lower apoptosis rate of nucleus pulposus cells (<i>P</i><0.01), and higher protein expression levels of ULK1, Beclin1, and LC3Ⅱ in nucleus pulposus tissue (<i>P</i><0.01). Rabbits in the blank control group exhibited generally normal organelle structures in nucleus pulposus tissues with few autophagic vacuoles, indicative of early stages of autophagy；while those in the model group showed disrupted organelle structures with cytoplasmic condensation and those in the EA group exhibited autophagosomes with double-membrane structures in nucleus pulposus tissues.</p><p><strong>Conclusions: </strong>EA promotes the expression of ULK1, Beclin1, and LC3Ⅱ proteins in nucleus pulposus tissues, reduces apoptosis of nucleus pulposus cells, and improves intervertebral disc degeneration.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"821-828"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation of clinical efficacy of acupuncture combined with bloodletting therapy and Qingwen Xiere decoction in mild to moderate COVID-19 patients.","authors":"Tian-Xin Jiang, Bao-Guo Wang, Qi-Qi Yang, Wen Liu, Shou-Liang Ma, Fei Li, Jun Yang","doi":"10.13702/j.1000-0607.20230316","DOIUrl":"10.13702/j.1000-0607.20230316","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the clinical efficacy and safety of acupuncture combined with bloodletting therapy and Qingwen Xiere decoction in mild to moderate COVID-19 patients.</p><p><strong>Methods: </strong>A total of 100 mild to moderate COVID-19 patients collected from December 2022 to February 2023 were randomly divided into control and observation groups, with 50 patients in each group. Patients in the control group received oral Qingwen Xiere decoction for 6 days. The observation group received acupuncture combined with bloodletting therapy in addition to oral Qingwen Xiere decoction, with the acupuncture (at Kongzui ［LU6］, Hegu ［LI4］, Quchi ［LI11］, Feishu ［BL13］, Zhongwan ［CV12］, Qihai ［CV6］, Yinlingquan ［SP9］) administered 30 min each day for 6 days, and bloodletting (at Shaoshang ［LU11］, Shangyang ［LI1］, Dazhui ［GV14］) administered every other day for 3 times. Traditional Chinese medicine syndrome scores and pulmonary CT scores were recorded before and after treatment. Serum contents of C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were measured using ELISA. Anxiety and depression degree were assessed using the Hamilton Depression Scale (HAMD) and Hamilton Anxiety Scale (HAMA). Safety of the treatments was evaluated in both groups.</p><p><strong>Results: </strong>Compared with before treatment, after treatment, the control group showed improvement in fever, dry cough, sore throat, and total traditional Chinese medicine syndrome scores (<i>P</i><0.01), but no significant improvement in muscle pain or fatigue；the observation group showed significant improvement in total traditional Chinese medicine syndrome and individual symptoms scores (<i>P</i><0.01)；both groups demonstrated reductions in pulmonary CT scores, HAMA score, HAMD score and serum contents of CRP and IL-6 (<i>P</i><0.01)；serum TNF-α content significantly decreased in the observation group (<i>P</i><0.01). All outcome measures were superior in the observation group to the control group (<i>P</i><0.01, <i>P</i><0.05). No adverse reactions were reported in either group.</p><p><strong>Conclusions: </strong>Acupuncture combined with bloodletting therapy and oral Qingwen Xiere decoction effectively improves clinical symptoms, alleviates pulmonary inflammatory injury, reduces inflammatory cytokine contents, and mitigates anxiety and depression in mild to moderate COVID-19 patients, and without adverse effects.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"845-850"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of placebo effect of acupuncture.","authors":"Yu-Ting Wang, Pei-Ming Zhang, Yu Dong, Bao-Chao Fan, Li-Ming Lu","doi":"10.13702/j.1000-0607.20230390","DOIUrl":"10.13702/j.1000-0607.20230390","url":null,"abstract":"<p><p>Acupuncture is a comprehensive therapy which takes the theory of traditional Chinese medicine as the core. Currently, there is much controversy surrounding the \"placebo effect\" of acupuncture： some people argue that acupuncture is a \"super placebo\", while others believe that the so-called placebo effect is just part of the therapeutic effect of acupuncture. To solve the controversy, this article clarified the definition, composition, and research purposes of \"placebo acupuncture\", pointed out its differences from \"sham acupuncture\", elucidated the relationship between the placebo effect and the intrinsic efficacy of acupuncture, distinguished the differences between the \"observed placebo effect\" and the \"real placebo effect\" in acupuncture clinical trials, and proposed the dilemmas and prospects of implementing \"placebo acupuncture\" and \"sham acupuncture\" contrasts at the current stage, in order to provide clues for optimizing randomized controlled trials of acupuncture.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"875-879"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New ideas for the development of acupuncture and moxibustion under the background of medicine-engineering translation.","authors":"Bing-Yuan Zhou, Cai-Feng Zhu, An-Qi Wu, Le Cheng, Jing Li, Xiao-Ya Cui","doi":"10.13702/j.1000-0607.20230936","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230936","url":null,"abstract":"<p><p>With the increasing value of translational medicine and medical engineering in the medical field, the collaborative innovation of acupuncture and engineering has become a trend. The mode and concept of the discipline of medicine-engineering junction can promote the development of translational research with acupuncture characteristics, including the rule of syndrome evolution, the application of meridians and acupoints, the exploration of treatment methods, the research of effect mechanism and the innovation of diagnosis and treatment. This paper put forward the future underlying research ideas, the existing questions and challenges for medicine-engineering junction, so as to provide the evidences for the clinical promotion of \"combination of medicine and engineering\" in the application of modern acupuncture and moxibustion.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"885-892"},"PeriodicalIF":0.0,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142355504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of penetration needling from \"Zhibian\" (BL54) to \"Shuidao\" (ST28) on SIRT1/PGC-1α/Nrf2 signaling pathway in rats with premature ovarian insufficiency].","authors":"Lu-Yun Yin, Hui-Min Feng, Fang Qiu, Jing Yan, Xiao-Fei Jin","doi":"10.13702/j.1000-0607.20230396","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230396","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of penetration needling from \"Zhibian\" (BL54) to \"Shuidao\"(ST28) on silencing information regulator 1 (SIRT1) /peroxisome proliferator-activated receptor-γ co-activator 1α (PGC-1α) /nuclear factor E2 related factor 2 (Nrf2) signaling in rats with premature ovarian insufficiency (POI), so as to explore its mechanisms underlying improvement of POI.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 48 female SD rats were equally and randomly allocated to blank control, POI model, shallow needling and penetration needling (from \"Zhibian\" ［BL54］ to \"Shuidao\" ［ST28］) groups. The POI model was established by intraperitoneal injection of cyclophosphamide (50 mg·kg&lt;sup&gt;-1&lt;/sup&gt;·d&lt;sup&gt;-1&lt;/sup&gt; on the 1&lt;sup&gt;st&lt;/sup&gt; day and 8 mg·kg&lt;sup&gt;-1&lt;/sup&gt;·d&lt;sup&gt;-1&lt;/sup&gt; from the 2&lt;sup&gt;nd&lt;/sup&gt; to 15&lt;sup&gt;th&lt;/sup&gt; day, for a total of 15 days). After successful modeling, for rats of the shallow needling group, a filiform needle was inserted into BL54 to a depth about 5-8 mm, and then retained for 30 min. And for rats of the penetration needling group, a filiform needle was inserted into BL54 area and advanced to the unilateral ST28 to a depth about 12-15 mm, and then retained for 30 min (bilateral acupoints were used at the same time). The treatments were conducted once daily, 6 times a week for 4 weeks. After the interventions, the contents of serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and anti-Müllerian hormone (AMH) were detected using ELISA, and the activity of superoxide dismutase (SOD), catalase (CAT) and content of malondialdehyde (MDA) in the ovarian tissue were detected using colorimetry. Histopathological changes of the ovarian tissue were observed after H.E. staining. The immunoactivities and expression levels of SIRT1, PGC-1α, and Nrf2 mRNA and protein in the ovarian tissues were detected using immunohistochemistry, quantitative real-time PCR and Western blot, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;After modeling, the rats' estrus cycles were disordered, contents of serum FSH and LH levels significantly increased, and the E2 level markedly decreased compared with those of the blank control group (&lt;i&gt;P&lt;/i&gt;&lt;0.01), indicating that the POI model was successfully established. Relevant to the blank control group, the model group had an increase in serum FSH and LH, ovarian MDA contents, and the number of atretic oocytes (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and a decrease in serum E2 and AMH contents, ovarian SOD and CAT activities, number of growing oocytes, immunoactivities and expressions of ovarian SIRT1, PGC-1α and Nrf2 protein and mRNA (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05). Following interventions, both the increased levels of serum FSH and LH and ovarian MDA contents, and the number of atretic oocytes, and the decreased levels of E2 and AMH contents, ovarian SOD and CAT activities, number of growing oocytes, immunoactivities and expressions of ovarian SIRT1, PGC-1α and Nrf2 protein and mRNA we","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 9","pages":"933-942"},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}