针刺研究最新文献

{"title":"Acupuncture alleviates neuro-inflammatory injury in rats with cognitive impairment induced by Parkinson's disease based on cGAS-STING signaling pathway.","authors":"Qi-Lin Liu, Bai-Yi Qiu, Jing Jin, Jiang-Hua Liu, Wen Zhang, Lei Jiang","doi":"10.13702/j.1000-0607.20230839","DOIUrl":"10.13702/j.1000-0607.20230839","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of acupuncture on neuro-inflammation in rats with cognitive impairment induced by Parkinson's disease (PD) based on the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway.</p><p><strong>Methods: </strong>SD rats were randomly grouped into sham operation, model, acupuncture, acupuncture+empty and acupuncture+cGAS over-expression groups, with 12 rats in each group. The PD model was established by injecting 6-hydroxydopamine (6-OHDA, 8 μg/rat) into the substantia nigra pars compacta of the left midbrain (AP:-5.2 mm, ML:2.1 mm, DV:-7.8 mm). Acupuncture was conducted for rats in the acupuncture, acupuncture+empty and acupuncture+cGAS over-expression groups, for 20 min, once daily; empty plasmid or cGAS over-expression plasmid was injected into the substantia nigra pars compacta of the left midbrain for rats of the acupuncture+empty or acupuncture+cGAS over-expression groups respectively, twice a week. The same volume of 0.9% sodium chloride solution was injected for rats in the sham operation, model and acupuncture groups, twice a week. All above interventions were performed for 2 weeks. The rotation behavior of rats was detected by apomorphine-induced rotation experiment. The cognitive function was detected by Morris water maze test and dark avoidance test. Nissl staining was used to detect the pathological morphology and the number of hippocampal neurons. TUNEL staining was used to detect the apoptosis rate of hippocampal neurons. The contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in serum and midbrain tissue were detected by ELISA. Western blot was used to detect the expression levels of cGAS and STING proteins in midbrain tissue.</p><p><strong>Results: </strong>Compared with the sham operation group, the rotation speed, the escape latency in the Morris water maze test and the number of errors in the dark avoidance test, the apoptosis rate of hippocampal neurons, the contents of TNF-α and IL-1β in serum and midbrain tissue, and the expression levels of cGAS and STING protein in midbrain tissue were increased (<i>P</i><0.05), while the times of crossing the original platform in the Morris water maze test, the latency in the dark avoidance test and the number of hippocampal neurons were decreased (<i>P</i><0.05) in the model group, the hippocampal neurons were disordered. All the indexes mentioned above were reserved in the acupuncture and acupuncture+empty groups (<i>P</i><0.05), and there was no significant difference of all indexes between these two groups. After injection of cGAS over-expression plasmid, compared with acupuncture group, the rotation speed, the escape latency in the Morris water maze test and the number of errors in the dark avoidance test, the apoptosis rate of hippocampal neurons, the contents of TNF-α and IL-1β in serum and midbrain tissue, and the expression levels of cGAS and STING protein in midbrain tissue were","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"159-166"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of electroacupuncture at \"Fenglong\"(ST40) and \"Zusanli\"(ST36) on the SIRT1/FOXO1 signaling pathway in non-alcoholic fatty liver disease model rats.","authors":"Xin-Yue Hu, Ya Luo, Fang Zu, Hua-Yu Zhang, Wen-Hui Zhen, Sha-Sha Xiao, Jie Wu, Jia-Jia Rao, Xiao-Fang Yang, Ning Zhang","doi":"10.13702/j.1000-0607.20240849","DOIUrl":"10.13702/j.1000-0607.20240849","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of electroacupuncture (EA) on liver function, lipid metabolism, hepatic histopathology, and the expression of molecules in the SIRT1/FOXO1 signaling pathway in rats with non-alcoholic fatty liver disease (NAFLD), as well as to explore its potential underlying mechanisms.</p><p><strong>Methods: </strong>A total of 13 SD rats were assigned to the blank group and fed a standard diet. An NAFLD model was established in 43 SD rats through a 12-week high-fat diet. Three rats from each group were randomly selected to confirm successful model establishment. After confirmation, rats in the modeling group were randomly divided into four groups:model group, EA group, EA+inhibitor group, and agonist group, with 10 rats in each group. The blank and model groups underwent immobilization three times per week for four weeks. The agonist group received intraperitoneal injections of the SIRT1 agonist resveratrol (200 mg/kg) three times per week for four weeks. The EA group received EA at \"Fenglong\" (ST40) and \"Zusanli\" (ST36) acupoints for 30 minutes, three times per week for four weeks. The EA+inhibitor group was administered the SIRT1 inhibitor EX527 (5 mg/kg) intraperitoneally, with the remaining treatment identical to that of the EA group. After the interventions, contents of serum high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglycerides (TG), as well as activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by using colorimetric method. Liver histopathology was assessed using hematoxylin-eosin (HE) and Oil Red O staining. The protein and mRNA expressions of SIRT1, FOXO1, and ABCA1 in liver tissue, as well as the protein expression of acetylated FOXO1 (AC-FOXO1), were detected using Western blot and PCR.</p><p><strong>Results: </strong>Compared with the blank group, the model group exhibited significantly decreased serum HDL-C contents (<i>P</i><0.05), along with increased contents of LDL-C, TC, TG, and activities of ALT and AST (<i>P</i><0.01, <i>P</i><0.05); histological analysis revealed disorganization of hepatocytes and pronounced fat vacuolization, additionally, the expressions of hepatic SIRT1, FOXO1, and ABCA1 proteins and mRNA were reduced (<i>P</i><0.05, <i>P</i><0.01), whereas AC-FOXO1 protein expression was elevated (<i>P</i><0.05). Compared with the model group, the EA and agonist groups demonstrated increased serum HDL-C contents (<i>P</i><0.05), along with decreased contents of LDL-C, TC, TG, and ALT and AST activities (<i>P</i><0.01, <i>P</i><0.05); histological results showed improved hepatocyte morphology and reduced steatosis, along with elevated expression of SIRT1, FOXO1, and ABCA1 proteins and mRNA (<i>P</i><0.05, <i>P</i><0.01) and decreased AC-FOXO1 protein expression (<i>P</i><0.05). Compared with the EA group, the EA+inhibitor group had significantly lower serum HDL-","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"150-158"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture of \"Jiaji\" (EX-B2) inhibits neuroinflammatory response and glial scar formation by regulating the expression of neutrophil extracellular traps in mice with spinal cord injury.","authors":"Jia-Pei Wang, Si-Qin Huang, Cheng-Lin Tang, Zhu-Xin Yang, Ming-Jiao Li, Cai Liao, Ke-Han Xing","doi":"10.13702/j.1000-0607.20240460","DOIUrl":"10.13702/j.1000-0607.20240460","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) on neuroinflammatory response and glial scar formation Via regulating expression of neutrophil extracellular traps (NETs) in mice with spinal cord injury (SCI).</p><p><strong>Methods: </strong>Sixty female C57BL/6 mice were randomized into sham operation, model, EA, DNase1 and EA+DNase1 groups, with 12 mice in each group. The SCI model was established by clamping the spinal cord with a serrefine after laminectomy at the 11^th thoracic vertebra (T11). EA (1.5 Hz/7.5 Hz, 1 mA) was applied to bilateral \"Jiaji\" (EX-B2, T10, T12) for 10 min, once a day for 14 consecutive days, for mice of the EA and EA+DNasel groups. Mice of the DNase1 and EA+DNase1 groups were intraperitoneally injected with NETs inhibitor (DNase1, 50 μg/mice), twice daily for 14 days. The motor function was evaluated by BBB score. Histopathological changes of the injured area of the spinal cord were determined by H.E. staining. The positive expressions of citrullinated histone H3 (citH3) and glial fibrillary acidic protein (GFAP) in the spinal cord of mice were observed by immunofluorescence. Western blot was used to detect the expressions of citH3, elastase (NE), myeloperoxidase (Mpo), cyclic guanosine monophosphate-adenylate synthase (cGAS), interferon gene stimulator (STING), GFAP and Neurocan in spinal cord tissue. The contents of inflammatory factors interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in spinal cord and serum were detected by ELISA .</p><p><strong>Results: </strong>After SCI, the BBB scores were obviously decreased (<i>P</i><0.05), while the protein relative expressions of citH3, NE, Mpo, cGAS, STING, GFAP, and Neurocan in spinal cord tissue, and the fluorescence intensities of citH3 and GFAP, as well as the contents of IL-1β and TNF-α in serum and spinal cord tissue were all increased in the model group relevant to the sham operation group (<i>P</i><0.05, <i>P</i><0.01). In the EA, DNase1 and EA+DNase1 groups, SCI-induced reduction of BBB scores, and increases of the protein relative expressions of citH3, NE, cGAS, STING, GFAP, and Neurocan, and the fluorescence intensities of citH3 and GFAP, as well as the contents of IL-1β and TNF-α were reversed relevant to the model group (<i>P</i><0.05, <i>P</i><0.01). The expression level of Mpo protein decreased (<i>P</i><0.05, <i>P</i><0.01) in spinal cord of rats in the EA and EA+DNasel groups relevant to the model group. The therapeutic effects of EA+DNase1 were better than EA or DNase1 (<i>P</i><0.05, <i>P</i><0.01). H.E. staining showed neuronal degeneration, nuclear pyknosis and inflammatory cell infiltration in the injured spinal cord tissue in the model group, which was relatively milder in the three intervention groups.</p><p><strong>Conclusions: </strong>EA of EX-B2 can alleviate neuroinflammatory reactions and glial scar formation in SCI mice, and improve the recovery of neurological function after SCI, which may","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"141-149"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis on the clinical advantages and the rules of acupoint selection in treatment of chronic obstructive pulmonary disease with the integration of acupuncture and medication based on data mining.","authors":"Chao-Yang Zhang, Yuan-Zhen Yang, Yu Luo, Fei-Fei Gao, Yi Guo, Tian-Yi Zhao, Shan-Shan Li, Zhi-Fang Xu","doi":"10.13702/j.1000-0607.20231122","DOIUrl":"10.13702/j.1000-0607.20231122","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the clinical advantages and the rules of acupoint selection in treatment of chronic obstructive pulmonary disease (COPD) with the integration of acupuncture and medication by means of frequency statistics and complex network analysis, so as to provide references for the standardized treatment with the integration of acupuncture and medication.</p><p><strong>Methods: </strong>Searched from PubMed, Web of Science, Embase, Ovid, CNKI, Wanfang, VIP, and SinoMed, the articles of clinical trials for COPD treated with the integration of acupuncture and medication were collected, dated from January 1, 2003 to September 1, 2023. Literature was screened according to the inclusion and exclusion criteria. Using frequency statistics, the clinical advantages of the integration of acupuncture and medication were explored. The database of acupoint prescriptions was established using Microsoft Excel 2019, the complex network model was constructed using SPSS18.0 and Gephi0.10, and the core acupoints and acupoint combinations were screened.</p><p><strong>Results: </strong>In the analysis of the clinical advantages of the combination of acupuncture and herbal medicine for the treatment of COPD, acupuncture alone contributed to the improvement of respiratory system indicators a total of 263 times, accounting for 51.77% of the total number of improved indicators, with the majority being improvements in resting lung function (184 times, 36.22%). In addition, acupuncture alone also showed certain improvement effects on clinical symptoms, cardiac function, and biochemical blood indicators in COPD patients. The combination of acupuncture and traditional Chinese medicine (TCM) contributed to the improvement of respiratory system indicators 90 times, accounting for 42.45% of the total improvement frequency, with the primary focus being on improvements in resting lung function (66 times, 31.13%), followed by improvements in clinical symptoms and biochemical blood indicators. The combination therapy for COPD mainly selected acupoints from the Bladder Meridian of Foot-Taiyang, with the combination of \"Shenshu (BL23) + Feishu (BL13)\" being the most frequently used acupoint pair. By performing \"k-core\" analysis and community network partitioning on the complex network diagram, three core acupoint clusters were identified.</p><p><strong>Conclusions: </strong>This article categorizes the clinical advantages of acupuncture and herbal medicine combination therapy for COPD into two types:the advantages of acupuncture alone and the advantages of acupuncture combined with TCM. Both can improve the function of target organs and enhance patients' lung function. Moreover, for other non-target organ indicators, both also have an improvement effect, demonstrating the comprehensive regulatory capacity of the combination of acupuncture and herbal medicine. In the clinical acupoint selection for the treatment of COPD with the combination of acupunctur","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"232-242"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on clinical and mechanism researches of acupuncture therapy for asthma.","authors":"Ting-Ting Wang, Gui-Hua Song","doi":"10.13702/j.1000-0607.20230637","DOIUrl":"10.13702/j.1000-0607.20230637","url":null,"abstract":"<p><p>Asthma is a chronic lung disease that occurs repeatedly and is hardly cured. Clinical studies have shown that acupuncture can effectively relieve asthma-related symptoms and is an ideal measure to treat asthma. The paper reviews the clinical and mechanism researches of acupuncture for asthma reported in China and other countries in recent 5 years, so as to provide the relevant evidences for the further study on asthma. The results showed that the mechanism of acupuncture in treatment of asthma may be related to modulating immune cytokines, ameliorating iron metabolism disorder, regulating the expression of neurotransmitter and receptor, promoting eosinophile apoptosis, inhibiting the expression of matrix metalloproteinase-9 and matrix metalloproteinase inhibitor-1, suppressing the expression of the related proteins and genes in type 2 innate lymphoid cells, inhibiting the signal pathways such as transforming growth factor-β1/Smad, mitogen-activated protein kinase, phosphatidylinositol 3-kinase/protein kinase B, signal transducer and activator of transcription, and nuclear factor-κB. Accordingly, the airway inflammatory response can be suppressed, the airway hyperreactivity be reduced, and the airway remodeling be relieved.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"217-223"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of electroacupuncture on the expressions of myelin-related proteins in the hippocampus of mice with Alzheimer's disease].","authors":"Jin-Quan Li, Wei-Guo Dong, Ruo-Lan Wen, Wan-Qing Guo, Chen-Guang Wen, Jun-Yan Huang, Shao-Min Yang, Lan Lin, Ling-Long Liu","doi":"10.13702/j.1000-0607.20231061","DOIUrl":"10.13702/j.1000-0607.20231061","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effects of electroacupuncture (EA) on anxiety-like behavior, learning and memory ability and expressions of myelin-related proteins in the hippocampus of senescence accelerated mice prone 8 (SAMP8), so as to explore the mechanism of EA in improving Alzheimer's disease (AD) from the perspective of myelin sheath injury and regeneration.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Twenty-four male SAMP8 mice were randomly and equally divided into model group and EA group. Twelve male SAMR1 mice of the same age as SAMP8 mice were used as the control group. The rats in the EA group received acupuncture at \"Baihui\" (GV20), \"Dazhui\" (GV14) and \"Shenshu\" (BL23), among which GV14 and BL23 received EA intervention (1.5-2 mA, 2 Hz), the intervention lasted for 20 min once daily for a total of 3 courses, with every 8 d as a course and an interval of 2 d between each 2 courses. The anxiety-like behavior of mice was observed by open field test. The learning and memory ability of mice was observed by Morris water maze test. The neuronal demyelination in the hippocampus of mice was observed after luxol fast blue(LFB) staining. The positive expressions of myelin basic protein (MBP) and myelin protein lipid protein (PLP) in hippocampal CA1 region were detected by immunofluorescence. The mRNA expression levels of MBP, chondroitin sulfate proteoglycan (NG2) and PLP, and the protein expression levels of myelin-associated proteins MBP, NG2, PLP, myelin-associated glycoprotein (MAG) and myelin oligodendrocyte glycoprotein (MOG) in the hippocampus were detected by qPCR and Western blot, respevtively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the control group, the activity distance and activity time in the central region in open field test of the model group were significantly shortened (&lt;i&gt;P&lt;/i&gt;&lt;0.01), the escape latency of the Morris water maze test was prolonged (&lt;i&gt;P&lt;/i&gt;&lt;0.01), the number of crossing the original platform and the residence time in the original platform quadrant were decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01). The LFB staining in the hippocampus was shallow, the myelin fibers were sparse, the positive expressions of MBP and PLP in the hippocampal CA1 region, and the mRNA expression levels of MBP, NG2 and PLP, as well as the protein expression levels of MBP, NG2, PLP, MAG and MOG in the hippocampus were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the model group than those of the control group. After the acupuncture or EA interventions, the activity distance and activity time in the central region in open field test of the mice in the EA group were significantly prolonged (&lt;i&gt;P&lt;/i&gt;&lt;0.01) , the escape latency of the Morris water maze test was shortened (&lt;i&gt;P&lt;/i&gt;&lt;0.01) , the number of crossing the original platform and the residence time in the original platform quadrant were increased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) , the LFB staining in the hippocampus was deepened, the myelin fibers were arranged more densely, the positive expression levels of M","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 1","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of heat-reinforcing needling on expression profile of LncRNAs in synovial tissues of rabbits with cold-syndrome type rheumatoid arthritis].","authors":"Xiao-Zheng DU, Li-Mei Liu, Cui Liu, Wei-Yao Jing, Bo Yuan, Xing-Hua Zhang, Feng-Fan Zhang, Xiang-Jun Li, Qiang Liu, Hai-Dong Wang","doi":"10.13702/j.1000-0607.20240026","DOIUrl":"10.13702/j.1000-0607.20240026","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of heat-reinforcing needling on the expression profile of long non-coding RNA (LncRNA) in the synovial tissue of rabbits with cold-syndrome type rheumatoid arthritis (RA), so as to explore its mechanisms underlying improvement of RA.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 18 New Zealand rabbits were randomly divided into normal, model and heat-reinforcing needling (acupuncture) groups, with 6 rabbits in each group. The RA model was established by injection of mixed solution (emulsifier) of ovalbumin powder(80 mg) + NaCl (20 mL) +Freund's complete adjuvant (20 mL), combined with cold compress (ice cube +crystal calcium chloride) of the knee-joint. In addition, rabbits of the normal and model groups received regular grasping and fixation in the same way as those of the acupuncture group. For rabbits of the acupuncture group, the heat-reinforcing needling stimulation was applied to bilateral \"Zusanli\" (ST36) for about 1 min, followed by retaining the needle for 30 min, once a day, for a total of 7 d. The rabbits' general condition was observed, and the knee joint circumference and pain threshold after intervention were measured. The knee-joint cavity effusion, synovial thickness, and internal blood flow signals were observed using a color Doppler ultrasound apparatus. Histopathological changes of synovium were observed using H.E. staining. The contents of serum tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-6 were detected using enzyme linked immunosorbent assay (ELISA), and the differential LncRNAs and biological functions in the synovial tissues were detected and analyzed using high-throughput sequencing technology. The expression levels of 5 significantly differentially expressed LncRNAs were verified using qPCR.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the normal group, the model group of rabbits showed a significant increase in the knee joint circumference (&lt;i&gt;P&lt;/i&gt;&lt;0.05) and the content of serum TNF-α, IL-1β, IL-6 (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and a significant decrease in the pain threshold (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and diffuse synovial hyperplasia of the synovial membrane, joint effusion and abnormal blood flow signals, proliferated and thickened synovial lining, with visible capillary formation beneath the lining and a large number of inflammatory cell clustering. In comparison with the model group, the knee joint circumference, and the serum contents of TNF-α, IL-1β and IL-6 were obviously decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while the pain threshold was notably increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and H.E. staining showed a reduction in the hyperplasia of synovial lining, with a milder inflammatory cell infiltration. High-throughput sequencing and bioinformatics analysis displayed that a total of 142 LncRNAs were found to have opposite expression trends between the acupuncture group and the model group, of which 83 LncRNAs were up-regulated in the model group &lt;i&gt;vs&lt;/i&gt; the normal group and down-regulated ","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 1","pages":"65-75"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress of acupuncture regulating enteric nervous system in Parkinson's disease].","authors":"Ke Xu, Shun Wang, Yan Bai, Yu-Lin Wang, Yuan Li, Cheng-Guang Zhang, Yue Shi, Tian-Jiao Wang, Ya-Ke Zhang","doi":"10.13702/j.1000-0607.20230775","DOIUrl":"10.13702/j.1000-0607.20230775","url":null,"abstract":"<p><p>Parkinson's disease (PD) is a neuro-degenerative disease that is common in middle-aged and elderly people, and its incidence is increasing year by year. Studies have shown that pathological products produced by a series of pathological cascade reactions in the enteric nervous system (ENS) of PD patients travel to the brain through the vagus nerve pathway in the microbial-gut-brain axis, and ultimately lead to the occurrence and development of PD. Acupuncture plays an important role in interfering with pathological products of ENS, restoring ecological balance of intestine and delaying PD process. In this paper, the effects of acupuncture on intestinal flora, production of related microbial products, α-synuclein, neuroinflammatory response and oxidative stress in patients or animal model with PD were reviewed.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 1","pages":"110-115"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture relieves neuropathic pain-induced anxio-depression-like behaviors by regulating glutamatergic neurons in the ventrolateral orbital cortex of mice].","authors":"Jia-Jia Gao, Zhi-Jun Diao, Wei Yuan, Si-Meng Xue, Xiao-Hang Yang, Yu-Wei Wu, Hai-Fa Qiao","doi":"10.13702/j.1000-0607.20230755","DOIUrl":"10.13702/j.1000-0607.20230755","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate whether electroacupuncture (EA) at \"Yanglingquan\" (GV34) and \"Xuanzhong\" (GB34) can alleviate anxio-depression-like behaviors induced by neuropathic pain and whether the effect of EA is in connection with the activity of glutamatergic neurons in the ventrolateral orbital cortex (VLO).</p><p><strong>Methods: </strong>The neuropathic pain model was established by common peroneal nerve ligation (CPNL). GV34 and GB34 were selected in EA treatment (2 Hz/15 Hz, 1 mA), 15 min each time, once a day for 7 d. Chemogenetic mixture viruses rAAV-VGLUT1-CRE-WPREs and rAAV-Ef1a-DIO-hM3Dq-mCherry-WPREs or rAAV-Ef1a-DIO-hM4Di-mCherry-WPREs were injected into the VLO, respectively. Clozapine-N-oxide (CNO) was injected intraperitoneally to regulate the activity of glutamatergic neurons in the VLO precisely. Mechanical withdrawal threshold (MWT) and paw withdrawal latency (PWL) were measured by dynamic plantar tactile instruments and plantar thermal nociceptor instruments. Anxio-depression-like behaviors were measured by behavioral tests, including open field test, elevated plus maze test, forced swimming test, and tail suspension test. The activation and inhibition levels of neurons were observed by immunofluorescence staining.</p><p><strong>Results: </strong>Compared with the sham group, the MWT and PWL of CPNL mice were significantly decreased (<i>P</i><0.001) on the 3^rd day after surgery and lasted until the 28^th day. After CPNL, the activity time spent in the central area of open field test reduced (<i>P</i><0.001), the percentage of entries and activity time in the open arm of elevated plus maze test were significantly reduced (<i>P</i><0.001, <i>P</i><0.05); the immobile duration of forced swimming test and tail suspension test were remarkably prolonged (<i>P</i><0.01, <i>P</i><0.05) of mice in the CPNL group. However, after EA treatment, compared with the CPNL group, the anxio-depression-like behaviors of the mice were alleviated (<i>P</i><0.05, <i>P</i><0.01, <i>P</i><0.001). Activating glutamatergic neurons of VLO produced an anti-anxio-depression effect (<i>P</i><0.05, <i>P<</i>0.01) in neuropathic pain mice. Compared with the inhibited+EA+saline group, inhibiting glutamatergic neurons of VLO reversed (<i>P</i><0.001, <i>P</i><0.01, <i>P</i><0.05) the effect of EA.</p><p><strong>Conclusions: </strong>EA at GV34 and GB34 can alleviate anxio-depression-like behaviors induced by neuropathic pain through the modulation of glutamatergic neuron activity in the VLO.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 1","pages":"3-13"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Observation on the clinical efficacy of acupuncture in treatment of oculomotor nerve palsy guided by Biaoben Genjie theory].","authors":"Ting Wang, Ying-Hua Chen, Na Yuan, Lin-Yi Guo","doi":"10.13702/j.1000-0607.20230895","DOIUrl":"10.13702/j.1000-0607.20230895","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the clinical effect of acupuncture in treatment of oculomotor nerve palsy guided by Biaoben Genjie theory (focusing on the distribution, connection and gathering of meridian <i>qi</i> in the body).</p><p><strong>Methods: </strong>A total of 62 patients with oculomotor nerve palsy were selected according to inclusion criteria of the trial. They were randomly divided into a control group and an observation group, with 31 cases in each group. The conventional treatment with Western medicine was used in the two groups. Besides, in the control group, the regular acupuncture was provided, and in the observation group, acupuncture was delivered under the guidance of Biaoben Genjie theory. In the 2 groups, the treatment was given once daily, for 30 min each time and one course of treatment was composed of 6 d. Six courses were required with the interval of 1 d between two courses. The degree of diplopia, the degree of strabismus and the score of traditional Chinese medicine (TCM) main symptoms were recorded before and after the treatment of the 2 groups separately, and the clinical efficacy was evaluated in each group.</p><p><strong>Results: </strong>The degrees of diplopia and strabismus decreased significantly (<i>P</i><0.05) compared with those before treatment in the 2 groups, and the degrees in the observation group were lower (<i>P</i><0.05) than those of the control group. The TCM main symptoms score of both groups were reduced significantly (<i>P</i><0.05) compared with those before treatment, and the score in the observation group was lower (<i>P</i><0.05) than that of the control group. The effective rate was 93.55% (29/31) in the observation group, better (<i>P</i><0.05) than that (83.87%, 26/31) of the control group.</p><p><strong>Conclusions: </strong>Acupuncture based on Biaoben Genjie theory can effectively relieve the ocular symptoms of the affected eyes in the patients with oculomotor nerve palsy, and the effect is better when compared with the regular acupuncture.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 1","pages":"90-95"},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}