针刺研究最新文献

{"title":"[Clinical observation on the effect of visual occlusion on pain sensation and pain emotion in patients with scapulohumeral periarthritis treated with acupuncture].","authors":"Yu-Ting Wei, Cui Ma, An-Guo Liu, Ying Cui, Shou-Xiang Tan, Xing-Ke Yan","doi":"10.13702/j.1000-0607.20240047","DOIUrl":"10.13702/j.1000-0607.20240047","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of visual occlusion acupuncture on shoulder pain, shoulder function, anxiety and depression in patients with acute scapulohumeral periarthritis, so as to evaluate the influence of psychological factors on the efficacy of acupuncture.</p><p><strong>Methods: </strong>A total of 74 patients with acute scapulohumeral periarthritis were randomly divided into heart-stable acupuncture group (17 cases, 1 dropped off), heart-stable covering acupuncture group (17 cases), heart-abnormal acupuncture group (20 cases, 1 dropped off) and heart-abnormal covering acupuncture group (20 cases) based on the results of acupuncture tension, anxiety and depression scores. Patients in the heart-stable acupuncture and heart-abnormal acupuncture groups were treated with acupuncture at Jianyu (LI15, affected side), Jianliao (SJ14, affected side), Jianzhen (SI9, affected side), Jianqian (EX-UE12, affected side), <i>Ashi</i> point, Yanglingquan (GB34, affected side), Tiaokou (ST38) to Chengshan (BL57, healthy side), Dazhui (GV14), Yinlingquan (SP9, affected side). While patients in the heart-stable covering acupuncture and heart-abnormal covering acupuncture groups wore eye mask before acupuncture treatment, and the same acupoints were needled. The treatment was conducted for 30 min, three times per week for 3 weeks. Before and after treatment, visual analogue scale (VAS) score, range of motion (ROM) of shoulder joint and activity of daily living (ADL) were observed for patients in the four groups. While the scores of self-rating anxiety scale (SAS) and self-rating depression scale (SDS) of patients in the heart-abnormal acupuncture and heart-abnormal covering acupuncture groups were observed. The therapeutic effects were evaluated after the treatment.</p><p><strong>Results: </strong>After the treatment, the VAS scores of the 4 groups were notably decreased (<i>P</i><0.05), while the ROM scores and ADL scores were obviously increased (<i>P</i><0.05). Compared with the heart-abnormal acupuncture group, the VAS score and SAS score were significantly lower in the heart-abnormal covering acupuncture group (<i>P</i><0.05), while the ADL score were significantly higher (<i>P</i><0.05). Compared with the heart-stable acupuncture group, the VAS score was increased (<i>P</i><0.05) and ADL score was decreased (<i>P</i><0.05) in the heart-abnormal acupuncture group.</p><p><strong>Conclusions: </strong>Acupuncture can effectively improve the pain sensation and pain emotion of patients with scapulohumeral periarthritis under different psychological states. The stability of psychological state is helpful for acupuncture to exert analgesic effect. Visual occlusion can strengthen the regulation of acupuncture on pain symptoms and anxiety in scapulohumeral periarthritis patients with abnormal psychological state.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"675-681"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Observation on the clinical efficacy on dyspepsia after ischemic stroke treated with elongated needle].","authors":"Wen-Bo Li, Xin-Ran Li, Hao Liu, Jian-Yun Zhang","doi":"10.13702/j.1000-0607.20240185","DOIUrl":"10.13702/j.1000-0607.20240185","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of elongated needle, filiform needle and medication for attenuating belching, nausea and vomiting in patients with dyspepsia after ischemic stroke.</p><p><strong>Methods: </strong>A total of 102 patients with dyspepsia after ischemic stroke were randomly divided into an elongated needle group (34 cases, 1 case dropped out), a filiform needle group (34 cases), and a medication group (34 cases, 2 cases dropped out). In the elongated needle group, the elongated needle with the length of 125 mm was inserted perpendicularly at Zhongwan (CV12), Qihai (CV6), Liangmen (ST21, bilateral) and Tianshu (ST25, bilateral). After inserted with the depth of 70 mm to 90 mm, the needle was lifted beneath the skin, with the depth of 45 mm to 75 mm. In the filiform needle group, the filiform needle, with the length of 40 mm was used and inserted perpendicularly at CV12, CV6, ST21 (bilateral) and ST25 (bilateral), with the depth of 20 mm to 30 mm. Acupuncture therapy was delivered once daily, 6 times a week for 2 weeks in these two groups. In the medication group, domperidone was prescribed orally, 10 mg each time, 3 times a day, for 2 weeks. The scores of Leeds dyspepsia questionnaire (LDQ), Nepean dyspepsia life quality index (NDLQI) and the dyspepsia symptom of TCM were evaluated before and after treatments. The clinical efficacy was compared among the 3 groups and the safety was evaluated.</p><p><strong>Results: </strong>After treatment, the scores of LDQ and TCM dyspepsia symptom decreased markedly compared with the baseline (<i>P</i><0.05), and NDLQI score increased (<i>P</i><0.05) in the 3 groups. After treatment, when compared with the filiform needle group and the medication group, the scores of LDQ and TCM dyspepsia symptom were decreased in the elongated needle group (<i>P</i><0.05) and the NDLQI score was increased (<i>P</i><0.05). The total effective rate was 93.94% (31/33), 76.47% (26/34) and 71.88% (23/32) in the elongated needle group, the filiform needle group and the medication group, respectively. The total effective rate in the elongated needle group was superior to either the filiform needle group or the medication group (<i>P</i><0.05). The serious adverse reaction was not found and the safety was high in the 3 groups.</p><p><strong>Conclusions: </strong>Elongated needling therapy with deep insertion can significantly relieve the symptoms of dyspepsia after ischemic stroke and promote the recovery in the patients. The effect of this therapy is superior to the treatment with either filiform needle or medication.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"682-688"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of acupotomy therapy on macrophage polarization and inhibition of synovial inflammation in rabbits with knee osteoarthritis based on the Notch1/Jagged1/Hes1 signaling pathway].","authors":"Wen-Ying Yu, Zhong-Biao Xiu, Ze-Hao Lin, Hong Liu, Liang-Zhi Zhang, Yu-Lin Li, Xiang-Long Feng, Jing Liu","doi":"10.13702/j.1000-0607.20240962","DOIUrl":"10.13702/j.1000-0607.20240962","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effects of acupotomy therapy on synovial inflammation, macrophage polarization, and protein expressions of drosophila double-wing edge notch homologous gene 1 (Notch1)/Jagged typical Notch ligand 1 (Jagged1)/Hes family BHLH transcription factor 1 (Hes1) signaling pathway, so as to explore its mechanisms underlying improvement of knee osteoarthritis (KOA).</p><p><strong>Methods: </strong>Male New Zealand rabbits were randomly divided into the blank, model and acupotomy groups (8 rabbits in each group). The KOA model was established by immobilizing the left hind limb in an extended position using a modified Videman method with a plaster cast for 6 weeks. After modeling, 3 meridian lesion points with significant reactions were selected from \"Hedingci\", \"Binwaishang\" \"Binneishang\" \"Chengfeijian\" \"Weiyangci\" and \"Yinlingci\" for acupotomy treatment, once a week for a total of 4 times. X-ray detection was performed after modeling. Behavioral changes in the affected knee joint of the rabbits were observed before and after the intervention and the Lequesne MG scores were recorded. Histopathological changes in the synovial tissue of the left knee joint of rabbits were observed and pathological scores were recorded after HE staining. The positive expressions of cluster differentiation 86 (CD86) and macrophage mannose receptor-1 (CD206) in the synovial tissue were detected by immunohistochemical staining. The mRNA expression levels of inducible nitric oxide synthase (iNOS), CD206, Jagged1, Notch1, arginase-1 (Arg-1) and Hes1 in the synovial tissue were detected by real-time qPCR, and the protein expression levels of Jagged1, Notch1, Arg-1, and Hes1 were detected by Western blot.</p><p><strong>Results: </strong>Compared with the blank group, the knee joint space of rabbits in the modeling group was significantly narrowed. Compared to the blank group, the model group showed significant increase in Lequesne MG scores (<i>P</i><0.01), pathological scores (<i>P</i><0.01), the positive expressions of CD86 and the mRNA expression levels of iNOS (<i>P</i><0.01), the mRNA and protein expression levels of Notch1, Jagged1 and Hes1 (<i>P</i><0.01, <i>P</i><0.05) in synovial tissue;and a decrease in the positive expression of CD206 (<i>P</i><0.01), the expression levels of CD206 mRNA, and Arg-1 mRNA and protein (<i>P</i><0.01, <i>P</i><0.05). After acupotomy intervention, modeling induced increase and decrease indexes mentioned above were reversed (<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Acupotomy can reduce the inflammatory response in synovial tissue of KOA rabbits by regulating the Notch1/Jagged1/Hes1 signaling pathway and promoting the polarization shift of synovial macrophages from M1-type to M2-type. This may be one of the mechanisms by which acupotomy treats KOA.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"649-657"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Complex network analysis on the clinical application characteristics of acupuncture and moxibustion in treatment of hiccups].","authors":"Qian Mo, Jing-Xuan Liu, Ya-Nan Li, Yi-Qing Zhang, Guo-Wu Lei, Jing Wen, Chun-Sheng Jia, Shen Zhang","doi":"10.13702/j.1000-0607.20230923","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20230923","url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the clinical application characteristics of acupuncture in treatment of hiccup, using data mining and complex network technology so as to provide better therapeutic regimen for the patients.</p><p><strong>Methods: </strong>Using computer retrieval, the articles of clinical study for the treatment of hiccup with acupuncture and moxibustion were searched in CNKI, Wanfang Database, VIP Database and PubMed from January 2002 to December 2022. The eligible articles were collected in compliance with the exclusion and inclusion criteria, and \"clinical literature database of acupuncture-moxibustion treatment of hiccup\" was established. The data were sorted in Microsoft Excel and analyzed using IBM SPSS Modeler 18.0 and Gephi-0.9.2.</p><p><strong>Results: </strong>A total of 920 articles were included, with 204 acupoints involved, and the selection frequency of acupoints was 9024 times. The top five meridians with the high use frequency included conception vessel, bladder meridian of foot-<i>taiyang</i>, stomach meridian of foot-<i>yangming</i>, pericardium meridian of hand-<i>jueyin</i> and spleen meridian of foot-<i>taiyin</i>. Based on the association rule analysis, \"Zusanli (ST36)→Neiguan (PC6)\", \"Zhongwan (CV12)→PC6\" and \"CV12→ST36\" were the most relevant acupoint combinations, which was coincident with the principles of acupoint selection, e.g. the selection of local acupoints, the selection of distal acupoints, and the combination of the acupoints located in the upper and lower parts of the body. Regarding the high use frequency of single therapy in treatment, filiform needle acupuncture, acupoint injection, moxibustion, auricular acupuncture and electroacupuncture were listed. Concerning to the compound therapy, \"filiform needle acupuncture→acupoint injection\" was the most correlated, followed by \"filiform needle acupuncture→moxibustion\" and \"filiform needle acupuncture→auricular acupuncture\". Through the K-core analytic hierarchy process and community analysis, 3 core acupoint prescriptions, 5 supplementary acupoint prescriptions based on syndrome differentiation, and 3 acupoint prescriptions for reinforcing and reducing were obtained.</p><p><strong>Conclusions: </strong>Using the complex network analysis, the rules of acupoint selection and the acupuncture-moxibustion techniques are found in treatment of hiccup. Acupoint selection is based on the acupoint location, syndrome differentiation and symptoms. Auricular acupuncture is predominated in terms of the micro-acupuncture system, and filiform needle acupuncture is the most common technique in treatment. For the application of compound therapy, the combination of filiform needle acupuncture with acupoint injection is the highest in use frequency.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"703-713"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of thermo-sensitive moxibustion intervention in regulating Th17/Treg immune imbalance in the rat model of allergic rhinitis].","authors":"Ling-Cong Guan, Jun Xiong, An-Ji Guo, Yi-Cheng Li, Shu-Jun Cui, Han-Ling Luo","doi":"10.13702/j.1000-0607.20240157","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240157","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To investigate the effects of thermo-sensitive moxibustion on helper T cells(Th)17/regulatory T cells(Treg) immune imbalance and related cytokines in the rat model of allergic rhinitis (AR).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Thirty 8-week-old SD rats were randomly divided into a blank group (&lt;i&gt;n&lt;/i&gt;=6), a model group (&lt;i&gt;n&lt;/i&gt;=6), a western medicine group (&lt;i&gt;n&lt;/i&gt;=6), and a moxibustion group (&lt;i&gt;n&lt;/i&gt;=12). AR model was established by intraperitoneal injection of ovalbumin and aluminium hydroxide suspension for 14 d followed by intranasal administration of 20 μL of 12% ovalbumin for 10 days. After successful modeling, rats in the western medicine group received 8 μL of fluticasone propionate intranasally for 21 days;the moxibustion group was divided into a thermo-sensitive moxibustion group(&lt;i&gt;n&lt;/i&gt;=4) and a traditional moxibustion group(&lt;i&gt;n&lt;/i&gt;=4) based on whether the increase in tail temperature detected by an infrared thermal imager was greater than 1 °C. Both moxibustion groups received moxibustion on \"Feishu\"(BL13) for 40 minutes, for 21 days. After the intervention, HE staining was used to observe the morphological changes of the nasal mucosa;ELISA was performed to measure serum interleukin(IL)-17A, IL-10, and transforming growth factor(TGF)-β1 contents;and immunohistochemical staining was used to observe the expression of IL-17A, retinoid-related orphan nuclear receptor γt(RORγt), IL-10, TGF-β1, and forkheadbox protein 3(FOXP3) in the nasal mucosa;flow cytometry was used to detect the percentage of Th17 and Treg in peripheral blood.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the blank group, rats in the model group displayed swelling and deformation of the nasal mucosa, significant eosinophil infiltration, and increased serum IL-17A contents along with increased expression of IL-17A and RORγt in the nasal mucosa, as well as a significant rise in peripheral blood Th17 percentage (&lt;i&gt;P&lt;/i&gt;&lt;0.001, &lt;i&gt;P&lt;/i&gt;&lt;0.05);while serum IL-10 and TGF-β1 contents, along with FOXP3, IL-10, and TGF-β1 expression in the nasal mucosa and peripheral blood Treg percentage were significantly reduced (&lt;i&gt;P&lt;/i&gt;&lt;0.05, &lt;i&gt;P&lt;/i&gt;&lt;0.001). Compared with the model group, no significant thickening or eosinophil infiltration was found in the nasal mucosa of the western medicine, traditional moxibustion, or thermo-sensitive moxibustion groups, with improvements of the above indicators observed in all groups to varying extents (&lt;i&gt;P&lt;/i&gt;&lt;0.001, &lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with the thermo-sensitive moxibustion group, the western medicine group showed decreased expression of FOXP3 in the nasal mucosa (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while serum IL-17A contents and nasal mucosal TGF-β1, RORγt, and IL-17A expressions were increased (&lt;i&gt;P&lt;/i&gt;&lt;0.001, &lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05), with an increase in peripheral blood Th17 percentage(&lt;i&gt;P&lt;/i&gt;&lt;0.01) and a decrease in Treg cells (&lt;i&gt;P&lt;/i&gt;&lt;0.01);Traditional moxibustion group showed significantly reduced le","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"658-665"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture of \"Jiaji\"(EX-B2) improves asthma by reducing airway inflammation and regulating PI3K/AKT/mTOR signaling pathway in rats with allergic asthma].","authors":"Tao Zhu, Jie Li, Hong-Ye Wan, Bu Chang, Yan-Ting Cheng, Yu-Fang Ji, Lai-Xi Ji","doi":"10.13702/j.1000-0607.20241336","DOIUrl":"10.13702/j.1000-0607.20241336","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of electroacupuncture (EA) at \"Jiaji\" (EX-B2) on asthma symptoms, airway inflammation and phosphatidylinositol 3-kinase (PI3K) / protein kinase B (AKT) / mammalian target of rapamycin (mTOR) signaling in rats with allergic asthma (AA), so as to explore its mechanisms underlying improvement of AA.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Forty male SD rats were randomly divided into blank control, AA model, EA and medication groups, with 10 rats in each group. The AA model was established by intraperitoneal injection of 1 mL 1% ovalbumin (OVA, grade V) suspension on the 1&lt;sup&gt;st&lt;/sup&gt; day and 7&lt;sup&gt;th&lt;/sup&gt; day and inhalation of 1% aerosol OVA (on the 15&lt;sup&gt;th&lt;/sup&gt; day) for 30 min, once daily for 2 weeks. Rats of the EA group received EA stimulation (2 Hz/15 Hz, 1-2 mA) of bilateral EX-B2 for 20 min, once daily for 2 weeks. Rats of the medication group received intraperitoneal injection of dexamethasone solution (0.5 mg/kg), once daily for 2 weeks. The nasal scratching or tickling (0-3 points) and asthma (0-9 points) behavior were graded, and their summation (behavior score) was used for assessing the severity of asthma. Histopathological changes of the lung tissue were assessed by giving pathological score after H.E. staining. The contents of IL-4, IL-5, IL-13 in the bronchoalveolar lavage fluid (BALF) and lgE in the serum were detected by ELISA. The immunoactivity of Beclin1 and microtubule-associated protein 1A/1B light chain 3B (LC3B) in the lung tissue was detected by immunohistochemistry. The ratio of expression levels of p-PI3K/PI3K, p-AKT/AKT, p-mTOR /mTOR proteins in the lung tissue were detected by Western blot.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the blank control group, the model group showed an increase in the behavior score (shortness of breath, wheezing and irritability, etc), pathological scores of the lung tissue, contents of IL-4, IL-5 and IL-13 in the BALF and IgE in the serum, the immunoactivity of Beclin1 and LC3B in the lung tissue (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and a decrease in the body weight, and the ratio of expression levels of p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR proteins in the lung tissue (&lt;i&gt;P&lt;/i&gt;&lt;0.01). In comparison with the model group, the behavior score, pathological score, the contents of IL-4, IL-5, IL-13 and IgE, and the immunoactivity of Beclin1 and LC3B were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05, &lt;i&gt;P&lt;/i&gt;&lt;0.01), while the ratio of expression levels of p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR proteins were considerably increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05, &lt;i&gt;P&lt;/i&gt;&lt;0.01) in both EA and medication groups. The body weight of rats was increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05) in the EA group compared to the model group. No significant differences were found between the EA and medication groups of all the indexes mentioned above.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;EA of EX-B2 can improve the symptoms of asthma in rats with AA, which may be associated with its functions in inhibiting the autopha","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"666-674"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effect of heat-reinforcing needling on the level of TRAF6-K63 ubiquitination and NF-κB pathway in the synovial tissue of knee joint in rabbits with rheumatoid arthritis of cold syndrome].","authors":"Feng-Fan Zhang, Xiao-Na Zhang, Xing-Hua Zhang, Fang-Hong Nian, Bo Yuan, Fang Li, Le-le Hu, Xiang-Jun Li, Hai-Dong Wang, Xiao-Zheng DU","doi":"10.13702/j.1000-0607.20240329","DOIUrl":"10.13702/j.1000-0607.20240329","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of heat-reinforcing needling on the level of tumor necrosis factor receptor associated factor 6 (TRAF6)-K63 ubiquitination and the nuclear factor-κB (NF-κB) pathway in the synovial tissue of the knee joints of a rabbit model with rheumatoid arthritis (RA) of cold syndrome, so as to explore the mechanism by which the heat-reinforcing needling inhibits inflammatory response in RA synovitis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Eight out of 32 rabbits were randomly selected and assigned to the normal group. The remaining rabbits were induced to establish the RA cold syndrome model using ovalbumin combined with complete Freund's adjuvant and low-temperature cryogenic methods. After modeling evaluation, the rabbits were randomly divided into model group, inhibitor group, and heat-reinforcing needling group, with 6 rabbits in each group. Rabbits in the inhibitor group received intraperitoneal injection of C25-140 solution (2.5 mg/kg), twice a day for 14 days. Rabbits in the heat-reinforcing needling group received acupuncture at \"Zusanli\"(ST36) according to the operation standard of the heat-reinforcing needling and the needle was retained for 30 min, once a day, for 14 days. After the intervention, knee joint circumference and pain threshold of the rabbits were measured. Color Doppler ultrasound was used to observe changes in knee joint imaging. HE staining was performed to observe morphological changes in the synovial tissue. Immunohistochemical method was used to observe the expression of inflammatory factors tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in the synovial tissue. Western blot was used to detect the protein expression of ubiquitin-conjugating enzyme E2 13 (Ubc13) and NF-κB p65 in the synovial tissue, and co-immunoprecipitation was used to detect the level of TRAF6-K63 ubiquitination in the synovial tissue.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the normal group, the model group showed a decreased pain threshold (&lt;i&gt;P&lt;/i&gt;&lt;0.05), increased knee joint circumference (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and increased average optical density of TNF-α, IL-1β, and IL-6 in the synovial tissue(&lt;i&gt;P&lt;/i&gt;&lt;0.05), as well as elevated protein expression of NF-κB p65 and TRAF6-K63 ubiquitination levels (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while Ubc13 protein expression was decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with the model group, the heat-reinforcing needling group and the inhibitor group had increased pain thresholds and reduced knee joint circumferences (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and significantly reduced average optical density of TNF-α, IL-1β, and IL-6 in the synovial tissue (&lt;i&gt;P&lt;/i&gt;&lt;0.05), as well as decreased protein expression of NF-κB p65, TRAF6-K63 ubiquitination levels (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while Ubc13 protein expression was increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared to the heat-reinforcing needling group, the inhibitor group showed reduced pain threshold (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and lower average optical density values of TNF-α, IL-1β, IL-","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"641-648"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on the mechanism of acupuncture based on microbiota-gut-brain axis].","authors":"Min Xing, Li-Na Qiao, Hong-Ye Wan, Yong-Sheng Yang","doi":"10.13702/j.1000-0607.20240145","DOIUrl":"10.13702/j.1000-0607.20240145","url":null,"abstract":"<p><p>Microbiota-gut-brain axis refers to a bidirectional pathway between gut microbiota and the central nervous systems via neural, immune, endocrine and metabolic pathways, and its dysfunction is the crucial factor implicated in many diseases. Acupuncture adjusts the microbiota-gut-brain axis through regulating the structure and metabolic state of gut microbiota, improving the intestinal microenvironment, restoring the stability of intestinal mucosal barrier and blood-brain barrier, stimulating vagus nerve, reducing inflammation and modulating neurotransmitters, so that nervous system diseases, mental diseases, digestive system diseases and metabolic diseases can be treated. With the latest research trends and the cutting-edge technologies considered, the studies in the future should deepen the mechanism of acupuncture on the physiological pathway for modulating the structure and composition of gut microbiota as well as its holistic elaboration.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"728-734"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of acupuncture on intracerebral mitochondrial function in mice with cerebral hemorrhage].","authors":"Hao Liu, Chen Ruan, Pei-Pei Feng, Xin-Wei Li, Zhong-Wei Guo, Ke-Lang Lou, Xiao-Qing Yu, Jia DU","doi":"10.13702/j.1000-0607.20240186","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240186","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of acupuncture on the recovery of neurological function in mice with intracerebral hemorrhage (ICH), so as to explore its mechanism of regulating mitochondria in brain tissue of ICH mice through the utilization of RNA sequencing and m6A methylation sequencing technology.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Twenty-four male C57BL/6J mice were ramondly divided into sham operation, model, and acupuncture groups (&lt;i&gt;n&lt;/i&gt;=8 in each group). The ICH model was established by injection of the rat's auto-blood (10 μL) into the right caudatum region. Acupuncture group received manual acupuncture stimulation of \"Baihui\" (GV20) for 30 min, once every 12 h for 7 d. After the intervention, the behavioral scores of mice in each group were evaluated. The contents of glutathione peroxidase malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in brain tissue were detected by ELISA. The N6-methyladenosine (m6A) modification genes of ICH mice brain tissue were analyzed using RNA and m6A sequencing. The protein expression level of mitochondrial outer membrane translocase 20 (TOMM20) was detected by Western blot. The mRNA expression levels of Ndufb3、Ndufb6、Uqcr11、Cox7c、Ndufv1、Ndufb11 and Cytb were detected by PCR.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the sham operation group, the suspension test score, balance walking test score, GSH-Px and SOD contents in the brain tissue were decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01), while the MDA content in the brain tissue was increased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the model group. After the intervention and compared with the model group, the scores of the suspension test, the balance walking test and the contents of GSH-Px and SOD were increased (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05), while the content of MDA was decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the acupuncture group. RNA sequencing showed that a total of 780 differentially expressed genes were screened in the model group compared with the sham operation group, a total of 1 097 differentially expressed genes were screened in the acupuncture group compared with the model group, and the shared differential genes in the sham group vs model group and model group vs acupuncture group were 342;and 50 common differential genes were obtained after intersection with mitochondrial-related genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the screened differentially expressed genes revealed that the above differential genes were mainly enriched in the oxidative phosphorylation pathway. PCR and Western blot verification results showed that compared with the sham operation group, the expression levels of Ndufb3, Ndufb6, Uqcr11, Cox7c, Ndufv1, Ndufb11 and Cytb mRNA, and the expression level of TOMM20 protein were decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the model group. All the indexes mentioned above were reserved (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the acupuncture group.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Acupuncture can impro","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"624-632"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Exploration of the mechanism of electroacupuncture at \"Jiaji\" (EX-B2) in treating neuronal apoptosis in rats with spinal cord injury based on the JAK2/STAT3 pathway].","authors":"Huan-Ying Jiang, Dong-Xu Zhang, Zhong-Ren Sun, Xue-Feng Jiao, Ming-Yuan Zhou, Fei Huang, Zi-Ru Luo, Mei-Qi Sun, Xin Kai, Hong-Na Yin","doi":"10.13702/j.1000-0607.20240070","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240070","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) at \"Jiaji\" (EX-B2) on inhibiting neuronal apoptosis in rats with spinal cord injury based on the Janus tyrosine kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway, so as to explore the underlying mechanism of EA in the treatment of spinal cord injury.</p><p><strong>Methods: </strong>A total of 36 SD rats were randomly divided into sham operation group, spinal cord injury group, and Jiaji EA group, with 12 rats in each group. The spinal cord injury model was established using Allen's method. The Jiaji EA group was treated with EA at EX-B2 (once a day, 30 min each time, for a total of 14 d). After the treatment, the Basso Beattie Bresnahan (BBB) score was used to evaluate the hindlimb motor function of rats in each group. HE staining was used to observe the morphological changes of spinal cord tissues. TUNEL staining was used to observe the pathological changes and neuronal apoptosis rate of the spinal cord. Western blot was used to detect the relative expression levels of phosphorylated (p)-JAK2, p-STAT3, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), and Caspase-3 proteins in the spinal cord.</p><p><strong>Results: </strong>Compared with the sham operation group at the same time point, the BBB score decreased (<i>P</i><0.01), the spinal cord tissue structure in the injury area was disordered, the cell body shrank, and the neuronal apoptosis rate increased (<i>P</i><0.01), the expression levels of p-JAK2, p-STAT3, Bax, and caspase-3 proteins increased (<i>P</i><0.01) while the expression level of Bcl-2 protein decreased (<i>P</i><0.01) in rats of the spinal cord injury group. Compared with the spinal cord injury group, the BBB score increased on the 7^th and 14^th day of intervention (<i>P</i><0.01), the spinal cord tissue structure tended to be normal, the degree of neuronal damage was reduced, and the apoptosis rate decreased (<i>P</i><0.01), the expression levels of p-JAK2, p-STAT3, Bax, and caspase-3 proteins decreased (<i>P</i><0.01, <i>P</i><0.05), while the expression level of Bcl-2 protein increased (<i>P</i><0.05) in rats of the Jiaji EA group.</p><p><strong>Conclusions: </strong>EA at EX-B2 can improve the motor function of rats with spinal cord injury, up-regulate the expressions of anti-apoptotic factors, down-regulate the expressions of pro-apoptotic factors, and reduce neuronal apoptosis in spinal cord injury. The mechanism may be related to the inhibition of the activation of the JAK2/STAT3 signaling pathway.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 6","pages":"633-640"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}