针刺研究最新文献

{"title":"[Study on the portrait construction of suitable population in the treatment of insomnia with \"Tongdu Tiaowei\" acupoint prescription].","authors":"Chi Wang, Shan Qin, Cheng-Yong Liu, Xiao-Qiu Wang, Kai Liu, Jing Jiang, En-Qi Liu, Ju-Guang Sun, Jin Lu, Min Ding, Wen-Zhong Wu","doi":"10.13702/j.1000-0607.20240534","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240534","url":null,"abstract":"<p><strong>Objectives: </strong>To establish a predictive model of acupuncture treatment of insomnia and to create a profile of suitable populations for acupuncture schemes, so as to help improve clinical efficacy.</p><p><strong>Methods: </strong>The data was sourced from a prospective clinical study on acupuncture treatment of insomnia by \"Tongdu Tiaowei\" acupoint prescription (Baihui [GV20], Yintang [EX-HN3], bilateral Shenmai [BL62] and bilateral Zhaohai [KI6]). Data from 113 insomnia patients were included in the analysis of the present study, with the reduction rate of the Pittsburgh Sleep Quality Index (PSQI) served as the overall clinical efficacy evaluation. First, the feature selection was performed using univariate logistic regression and Boruta algorithm, and the prediction accuracy of the three boosting algorithms - adaptive boosting, gradient boosting, and extreme gradient boosting (XGBoost) - was compared for selecting the best algorithm. The grid search and ten-fold cross-validation were used to optimize the hyperparameters of the best algorithm. The optimal dataset partitioning method was selected using stratified random partitioning, and the best cut-off value was determined based on the Youden index. The predictive model for the therapeutic efficacy was constructed and its performance was evaluated. Finally, SHAP (shapley additive explanation) analysis was used to visually interpret the model.</p><p><strong>Results: </strong>The features included in the model were the proportion of stage N1 to total sleep duration, the proportion of stage N2 to total sleep duration, R latency from lights out, stage N2 latency from lights out, the awake time after sleep onset, PSQI sleep efficiency score, and the presence of an old tongue (a tongue picture of a dry, rough texture and an old body). XGBoost was identified as the best algorithm, with the optimal probability threshold of 0.76, a corresponding precision of 0.91, a recall of 0.91, a F1 score of 0.91, an accuracy of 0.91, and an area under curve (AUC) of 0.82. Patients who meet the following conditions are more likely to respond to \"Tongdu Tiaowei\" acuoint stimulation:the proportion of N1 phase was about 6%-70% of the total sleep duration, N2 phase latency was less than about 40 min from the time when the lights were off, the wakefulness time was less than about 75 min or 100-300 min after falling asleep, the R phase latency was more than about 75 min from the time when the lights were off. The N2 phase were about 20%-50% of the total sleep duration, PSQI sleep efficiency score was 2 or 3, and there was no appearance of \"old tongue\".</p><p><strong>Conclusions: </strong>The predictive model of the efficacy of acupuncture treatment for insomnia established using XGBoost, along with the preliminary profile of the suitable population constructed using SHAP, provides a reliable auxiliary decision-making tool for acupuncture treatment of insomnia.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"954-964"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture alleviates neuropathic pain hyperalgesia and anxiety-like behaviors via the dorsal raphe nucleus-basolateral amygdala circuit].","authors":"Zong-Lin Wu, Yun-Yun Xu, Yue-Rong Chen, Zhe Liu, Si-Yuan Tong, Lin-Bao Wang, Liang Chen, Zhan-Peng Wang, Jian-Qiao Fang, Yuan-Yuan Wu","doi":"10.13702/j.1000-0607.20250181","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20250181","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to investigate the intervention effects of electroacupuncture on neuropathic pain and anxiety-like behavior, and to explore the underlying mechanisms of the DRN^5-HT-BLA (dorsal raphe nucleus serotoninergic - basolateral amygdala) neural circuit.</p><p><strong>Methods: </strong>Male C57BL/6J mice were selected, and a neuropathic pain model was established through spared nerve injury (SNI) surgery. Electroacupuncture (100 Hz, 0.3 mA, 30 min) was applied to bilateral \"Zusanli\" (ST36) and \"Sanyinjiao\"(SP6). Anxiety-like behavior was assessed using the elevated plus maze (EPM) and open field (OF) tests, while mechanical allodynia was evaluated using the Von-Frey test. Viral tracing was used to confirm the anatomical connection between DRN^5-HT and BLA. Immunofluorescence was used to assess the activity of 5-HT neurons in the DRN. Chemogenetic and calcium imaging techniques were employed to observe changes in the activity of 5-HT neurons in the DRN of DRN^5-HT-BLA. Chemogenetics were used to observe the effect of inhibiting DRN^5-HT-BLA on electroacupuncture.</p><p><strong>Results: </strong>SNI model mice showed a significant decrease in mechanical pain threshold (<i>P</i><0.000 1) and exhibited marked anxiety-like behavior in both the EPM and OF tests (<i>P</i><0.01, <i>P</i><0.05). Compared to the model group, electroacupuncture significantly increased the mechanical pain threshold in the electroacupuncture group (<i>P</i><0.000 1). The EPM and OF results indicated that electroacupuncture significantly reduced anxiety-like behavior (<i>P</i><0.05, <i>P</i><0.001) without affecting spontaneous activity. Virus tracing results confirmed the anatomical connection between 5-HT neurons in the DRN and the BLA. Immunofluorescence results showed that the activity of 5-HT neurons in the DRN was significantly reduced (<i>P</i><0.000 1) in SNI model mice, which was significantly reversed by electroacupuncture (<i>P</i><0.01). Calcium imaging revealed that in SNI model mice, the excitability of 5-HT neurons in the DRN^5-HT-BLA circuit was decreased, and electroacupuncture treatment restored their excitability. After chemogenetic inhibition of the DRN^5-HT-BLA circuit, electroacupuncture failed to improve mechanical allodynia and anxiety-like behavior in the SNI model mice (<i>P</i><0.05, <i>P</i><0.001, <i>P</i><0.01).</p><p><strong>Conclusions: </strong>5-HT neurons in the DRN^5-HT-BLA circuit play a crucial role in the mechanical allodynia and associated anxiety-like behavior in neuropathic pain. Electroacupuncture activates this circuit, enhancing the activity of 5-HT neurons in the DRN, thereby alleviating mechanical allodynia and anxiety-like behavior in SNI model mice.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"851-861"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on the analgesic effect of electroacupuncture at different intensities on the primary input level in mice with inflammatory muscle pain based on <i>in vivo</i> calcium imaging].","authors":"Ni-Nan Zhang, Yu-Shan Zhang, Zhi-Yun Zhang, Xiang-Hong Jing","doi":"10.13702/j.1000-0607.20250186","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20250186","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the differential analgesic effects of electroacupuncture (EA) at different intensities on calcium activity in dorsal root ganglia (DRG) neurons induced by mechanical, thermal, and cold stimuli in a state of muscular inflammatory pain, so as to preliminarily reveal the peripheral integration characteristics of acupuncture analgesia mechanisms.</p><p><strong>Methods: </strong>The inflammatory muscle pain model was established by injection of complete Freund's adjuvant (CFA) into the right tibialis anterior muscle. EA (0.5 mA or 1 mA, 2 Hz) was applied to \"Zusanli\" (ST36) on the right side for 10 min. Before and after the EA, mice were given body surface stimulation of brush, clamp and different temperature, while simultaneously recording DRG neuron calcium activity during stimulation. The effects of different stimulation methods before and after EA on the change in fluorescence intensity (ΔF/F0) and the percentage of activated DRG neurons were compared.</p><p><strong>Results: </strong>In the clamp hind paw stimulation, both 0.5 mA and 1 mA EA could reduce the fluorescence intensity of neurons, and the effect of 1 mA was better than that of 0.5 mA (<i>P</i><0.001). Specifically, 0.5 mA EA decreased the percentage of medium and large neurons (<i>P</i><0.05), while 1 mA EA decreased the percentage of small, medium, and large neurons (<i>P</i><0.05, <i>P</i><0.01), and the effect of 1 mA was better than that of 0.5 mA in reducing the percentage of small and medium neurons (<i>P</i><0.01). In the stimulation of clamping tibialis anterior muscle, both EA intensities could reduce the fluorescence intensity of neurons (<i>P</i><0.05, <i>P</i><0.000 1), and the effect of 1 mA was better than that of 0.5 mA (<i>P</i><0.000 1), and 1 mA EA could reduce the percentage of small and medium neurons (<i>P</i><0.05). In the brush stimulation, both EA intensities could reduce the fluorescence intensity of neurons (<i>P</i><0.000 1), and the effect of 1 mA was better than that of 0.5 mA (<i>P</i><0.000 1), and 0.5 mA EA decreased the percentage of medium and large neurons (<i>P</i><0.05), while 1 mA EA decreased the percentage of small, medium and large neurons (<i>P</i><0.05, <i>P</i><0.01). However, in the temperature stimulation (43, 51, 4 ℃), there were no obvious changes in the fluorescence intensity and the percentage of activated neurons in different intensities of EA.</p><p><strong>Conclusions: </strong>Both 0.5 mA and 1 mA EA intervention at ST36 can inhibit the reactivity of DRG neurons to mechanical stimulation, with higher intensity EA showing more significant effects.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"880-888"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture of \"Tianshu\" (ST25) alleviates ulcerative colitis by activating sacral parasympathetic postganglionic neurons of the pelvic ganglion in mice].","authors":"Min Luo, Zheng-Yang Qu, Tong Li, Xiao-Yu Liu, Ya-Jing Li, Yang-Shuai Su, Xiang-Hong Jing","doi":"10.13702/j.1000-0607.20250151","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20250151","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) of bilateral \"Tianshu\" (ST25) on ulcerative colitis (UC) in mice, and further investigate its related neural circuits including colonic-associated sympathetic and sacral parasympathetic postganglionic components.</p><p><strong>Methods: </strong>Thirty C57BL/6J male mice were randomly divided into normal control, model and EA groups, with 10 mice in each group. The UC model was established by free access to 3% dextran sulfate sodium (DSS) solution for 7 consecutive days. Under isoflurane inhalation anesthesia on day 1, 3, 5, and 7 of modeling, mice of the EA group received EA stimulation of bilateral ST25 (2 mA, 10 Hz) for 30 min each time. The colonic histopathological changes were assessed by HE staining, and the body weight of mice was recorded. The relative abundance values of acetylcholine (ACh) and norepinephrine (NE) in the distal colon tissues were detected by using high performance liquid chromatography-mass spectrometry. In addition, six male C57BL/6J mice were randomly and equally divided into model and EA groups. On the fifth day of modeling, mice in the EA group received once EA stimulation with the same operations above, while mice in the model group were only anesthestized without EA. Immunofluorescence staining was used to examine the expression of c-Fos protein of the tyrosine hydroxylase-positive (TH⁺) neurons in the superior mesenteric ganglion (SMG), inferior mesenteric ganglion (IMG), and vesicular acetylcholine transporter-positive (VAChT⁺) neurons in the pelvic ganglion (PG).</p><p><strong>Results: </strong>From day 7 to 9 of DSS modeling, the model group showed significant decrease of body weight compared to the normal control group (<i>P</i><0.001), and the EA group showed a significantly lower reduction in body weight in contrast to the model group (<i>P</i><0.01, <i>P</i><0.05). The histopathological score (thickening of the colon wall, injury of the lamina propria structure, submucosal edema, and inflammatory cell infiltration) was significantly increased after modeling (<i>P</i><0.000 1) and obviously improved after EA (<i>P</i><0.001). Compared with the normal group, the relative abundance of ACh in the distal colon of the model group decreased significantly (<i>P</i><0.05), which increased significantly in the EA group (<i>P</i><0.05) when compared with the model group. No significant differences were found among the 3 groups in the relative abundance value of NE in the distal colonic tissues. Results of immunofluorescence staining showed that, compared with the model group, there were no statistically significant differences in the expression of c-Fos in TH⁺ neurons of SMG and IMG (sympathetic postganglionic component innervating the colon tissue) in the EA group. However, compared with the model group, the number of VAChT⁺ neurons that express c-Fos in PG of the EA group increased significant","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"872-879"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research on the prescription characteristics in treatment of acute and chronic urticaria with integrative acupuncture and medicines based on data mining technology].","authors":"Guang-Ran He, Qian Mo, Chun-Sheng Jia","doi":"10.13702/j.1000-0607.20240391","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240391","url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the characteristics of point prescriptions for acute and chronic urticaria treated with acupuncture using complex network and data mining, so as to provide the basis for acupoint selection and the approaches to clinical treatment of acute and chronic urticaria.</p><p><strong>Methods: </strong>The clinical trials of acupuncture in treatment of urticaria were systematically searched from China National Knowledge Infrastructure (CNKI), VIP database (VIP), Wanfang database (WanFang), SinoMed and PubMed. According to the screening criteria, the \"database of acupuncture for acute and chronic urticaria\" was established. IBM SPSS Modeler18.0, Cytoscape3.9.0, Origin2021, Gephi0.9.2 and other softwares were used for frequency analysis, association rule analysis and cluster analysis.</p><p><strong>Results: </strong>A total of 352 articles were included, and 158 acupuncture prescriptions were extracted, involving 76 points. The analysis focused on main acupoints, and the laws of acupoint selection and medications based on syndrome differentiation. The results showed that the meridian with the highest use frequency of acupuncture was the bladder meridian of foot-<i>taiyang</i>. The top 6 acupoints with the highest use frequency included Quchi (LI11), Xuehai (SP10), Zusanli (ST36), Sanyinjiao (SP6), Geshu (BL17) and Feishu (BL13). Acupuncture was delivered for urticaria in terms of \"syndrome differentiation and treatment\" to determine the principle, and acupuncture was combined with medication as the main regimen. Among reinforcing and reducing techniques, the neutral supplementation and drainage method is the most frequently used. In the acupoint and herbal prescriptions for combined acupuncture-medication therapy of chronic urticaria:the 3 strongest acupoint pairings were Quchi (LI11) and Xuehai (SP10), Quchi (LI11) and Zusanli (ST36), and Xuehai (SP10) and Sanyinjiao (SP6). The 3 strongest herb pairs were Jingjie (<i>Schizonepeta</i>) and Fangfeng (<i>Saposhnikovia</i>), Gancao (<i>Licorice</i>) and Fangfeng (<i>Saposhnikovia</i>), and Danggui (<i>Angelica sinensis</i>) and Fangfeng (<i>Saposhnikovia</i>). The three strongest herb-acupoint associations were Xuehai (SP10) and Fangfeng (<i>Saposhnikovia</i>), Quchi (LI11) and Fangfeng (<i>Saposhnikovia</i>), and Zusanli (ST36) and Fangfeng (<i>Saposhnikovia</i>).</p><p><strong>Conclusions: </strong>Acupuncture is commonly combined with medication in treatment of acute and chronic urticaria. The combined therapy can solve the insufficiency presented in treatment with either simple delivery of acupuncture or Chinese herbal medicine, give a full play to the advantages of acupuncture and Chinese medicine, and advance the therapeutic effect.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"974-982"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Decoding acupuncture's modulation effects on intracerebral neural circuits through tri-dimensional perspectives: brain region activation, functional connectivity, and synaptic transmission].","authors":"Yi-Jun Hu, Teng He, Hao-Shuang Li, Yong-Jun Chen","doi":"10.13702/j.1000-0607.20250296","DOIUrl":"10.13702/j.1000-0607.20250296","url":null,"abstract":"<p><p>An increasing number of studies indicate that the modulation effect of acupuncture on intracerebral neural circuits is a crucial pathway through which acupuncture exerts its therapeutic effects. Among these, brain region activation, functional connectivity, and synaptic transmission collectively form the three dimensions of acupuncture modulation of neural circuits. Brain region activation reflects the activity state of key nodes in neural circuits, functional connectivity focuses on the intensity and patterns of information exchange within neural circuits, and snaptic transmission examines molecular-level changes during signal projection in neural circuits. To systematically elucidate the mechanisms of acupuncture, this paper first analyzed the impact of acupuncture on these three dimensions using commonly used points such as Quchi (LI11) as examples. Then, it explored the multi-dimensional regulatory effects of acupuncture on neural circuits related to clinically advantageous conditions like pain, mood disorders, and gastrointestinal dysfunction. Finally, it proposed issues and potential solutions for the multi-dimensional analysis of acupuncture-related neural circuits, aiming to provide insights for advancing research on acupuncture mechanisms and guiding further precision treatment.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"889-897"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture regulates blood glucose dysregulation by up-regulating IGF1-mediated PI3K/Akt signaling pathway in mice with circadian rhythm disorder].","authors":"Jia-Rui Miao, Xu Fan","doi":"10.13702/j.1000-0607.20240183","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240183","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To investigate the mechanism of electroacupuncture (EA) underlying improvement of abnormal blood glucose in mice with disturbance of peripheral biological clock.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;C57BL/6J mice (half male and half female) were randomly divided into normal control (&lt;i&gt;n&lt;/i&gt;=26), model (&lt;i&gt;n&lt;/i&gt;=26), EA (&lt;i&gt;n&lt;/i&gt;=26) and EA+ inhibitor (&lt;i&gt;n&lt;/i&gt;=8) groups. The circadian rhythm disturbance model was established by subjecting the mice to constant light (12-h light-light [LL] cycle) for 4 weeks. EA (4 Hz/20 Hz, 0.2 mA) was applied to bilateral \"Ganshu\" (BL18) for 15 min, once daily for 8 weeks. The mice of the EA+inhibitor group were given LY294002 (40 μmol/L, 10 μL) by intraperitoneal injection for blocking activities of phosphatidylinositol-3-kinase (PI3K) signaling pathway, once every other day for 8 weeks. After the intervention, the fasting plasma glucose (FPG) was measured, and fasting serum insulin (FINS) and the circadian rhythm of liver insulin-like growth factor 1 (IGF1) were observed by measuring their contents with ELISA. The insulin resistance was evaluated by homeostasis model assessment of insulin resistance (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI). The histopathological changes of the liver tissue were observed by H.E. staining. The changes of liver glycogen granule deposition were observed by PAS staining. The immunoactivity of forkhead box protein O1 (FoxO1) and glycogen synthase kinase 3β(GSK3β) in the liver tissue was detected by immunofluorescence technique. The expression levels of IGF1, IGF1R, FoxO1, GSK3β, phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6 phosphatase(G6Pase) mRNA and protein kinase B (Akt) and phosphorylated (p)-Akt proteins in the liver tissue were detected by qPCR and Western blot, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In comparison with the normal control group, daily water intake, the contents of FPG, FINS and HOMA-IR, the immunoactivity of GSK3β and FoxO1 in liver tissue, and the expression levels of IGF1R, GSK3β, FoxO1, PEPCK and G6Pase mRNA in liver tissue were significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05), while the serum QUICKI, ratio of p-PI3K/PI3K and p-Akt/Akt, and expression of IGF1 mRNA in liver tissue obviously decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) of mice in the model group. Compared with the model group, all the above indicators were significantly reversed in the EA group (&lt;i&gt;P&lt;/i&gt;&lt;0.05, &lt;i&gt;P&lt;/i&gt;&lt;0.01). After administration of the inhibitor LY294002 of PI3K signaling, the effects of EA in up-regulating the ratio of p-PI3K/PI3K and p-Akt/Akt, and in down-regulating the immunoactivity of GSK3β and FoxO1, and the expression levels of GSK3β, FoxO1 and G6Pase mRNA in liver tissue were eliminated (&lt;i&gt;P&lt;/i&gt;&lt;0.05). H.E. staining showed irregular arrangement of the hepatocytes with diffuse swelling, loose connections between hepatocytes, fat vacuoles of different sizes in the cytoplasm, and diffuse steatosis in some mice ","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"908-918"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture at \"Lianquan\" (CV23) improves post-stroke dysphagia via hypoglossal nucleus].","authors":"Wen-Hui DI, Ke-Ni Huang, Li-Chen Yi, Hao-Xi Long, Xuan-Hao Liang, Jun Gu, Lin Liu, En-Zhe Xu, Neng-Gui Xu, Lu-Lu Yao","doi":"10.13702/j.1000-0607.20250444","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20250444","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To investigate the mechanisms by which the hypoglossal nucleus (12N) modulates swallowing-related activities and mediates the therapeutic effects of electroacupuncture (EA) at \"Lianquan\" (CV23) in alleviating post-stroke dysphagia (PSD).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Tracer viruses were used to investigate the brain regions that directly project to CV23. C57BL/6J mice were divided into 7 groups:control, model, model+EA, normal+12N inhibition control, normal+12N inhibition, model+EA+12N inhibition control, and model+EA+12N inhibition groups (7 mice/group). Three additional C57BL/6J mice were used for the virus tracing experiment. A photochemical thrombosis model was established in C57BL/6J mice to simulate PSD, by intraperitoneally injecting 0.2 mL of 1.5% rose bengal solution combined with laser irradiation to induce ischemic pathological changes in the primary motor cortex;EA at CV23 (2 Hz, 1 mA, for 15 minutes, once in total) to study the effect of electroacupuncture on swallowing function in PSD mice. Chemogenetic viral inhibition was applied to suppress neuronal activity in the 12N, and changes in vocal fold movements and swallowing electromyography were observed under physiological conditions, by laryngoscopy and electrophysiological recorder respectively. After PSD model establishment, EA combined with chemogenetic inhibition of 12N was followed to observe the effect of chemogenetic inhibition of 12N on EA at CV23 in improving PSD. Tracer viruses were used to trace the upper brain region of the 12N.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The 12N directly projected to the surrounding tissues of CV23. Compared with the control group, in the model group, food residues were observed in the pharyngeal cavity and piriform recesses, along with prolonged vocal cord movement cycle time, a reduced number of vocal cord movement cycles within 10 seconds (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and decreased electromyographic area under the curve as well as reduced number of swallows (&lt;i&gt;P&lt;/i&gt;&lt;0.001, &lt;i&gt;P&lt;/i&gt;&lt;0.01). Compared with the model group, the model+EA group showed shortened vocal cord movement cycle time, an increased number of vocal cord movement cycles within 10 seconds (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and an enlarged electromyographic area under the curve (&lt;i&gt;P&lt;/i&gt;&lt;0.001). Physiologically, compared with the normal+12N inhibition control group, the normal+12N inhibition group showed a reduction in the area under the swallowing electromyography curve and a decrease in the number of swallows (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Pathologically, laryngoscopy showed that compared with the model+EA+12N inhibition control group, the vocal fold movement cycle time of the model+EA+12N inhibition group was prolonged, and the number of movement cycles within 10 seconds was reduced (&lt;i&gt;P&lt;/i&gt;&lt;0.01), the area under the swallowing electromyography curve was decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The upstream brain regions of the 12N projecting to the surrounding tissues of CV23 were mainly identified i","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"862-871"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of moxibustion on SIRT1/eNOS signaling pathway in ApoE^-/- atherosclerotic mice].","authors":"Xian-Ming Wu, Ning Zhang, Zhou Zhu, Xiao-Fang Yang","doi":"10.13702/j.1000-0607.20240618","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240618","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of moxibustion on pathological structure and ultrastructural changes of thoracic aorta, the expression of silent information regulator 1 (SIRT1) and endothelial nitric oxide synthase (eNOS) in ApoE^-/- atherosclerosis (AS) mice, so as to explore its possible mechanism in preventing and treating AS.</p><p><strong>Methods: </strong>Ten C57BL/6J mice were fed a normal diet as the control group, and 30 ApoE^-/- mice were fed a high-fat diet to establish the AS model, which were randomly divided into the model group, moxibustion group, and moxibustion+EX527 group, with 10 mice in each group. Mice in the moxibustion group received mild moxibustion treatment at \"Danzhong\"(CV17), \"Shenque\"(CV8), \"Neiguan\"(PC6, bilateral), and \"Xuehai\" (SP10, bilateral) for 30 min, the mice in the moxibustion+EX527 group were given intraperitoneal injection of EX527 (10 mg/kg) 30 min before moxibustion, with both treatments given once daily, 5 times a week, with a total intervention period of 12 weeks. After the intervention, the contents of nitric oxide (NO) and endothelin-1 (ET-1) in serum were detected by ELISA. HE staining and transmission electron microscopy (TEM) were used to observe the pathological structure and ultrastructural changes of thoracic aorta. The expression of endomucin of thoracic aorta was observed by immunofluorescence staining. The protein and mRNA expression levels of SIRT1 and eNOS in thoracic aorta were detected by Western blot and real-time fluorescence quantitative PCR, respectively.</p><p><strong>Results: </strong>Compared with the control group, the content of serum NO was decreased (<i>P</i><0.05), while the content of serum ET-1 was increased (<i>P</i><0.05);the fluorescence intensity of endomucin, the protein and mRNA expression of SIRT1 and eNOS in thoracic aorta were significantly decreased (<i>P</i><0.05) in the model group. Compared with the model and moxibustion+EX527 groups, the moxibustion group showed a significantly increase in the serum NO contents (<i>P</i><0.05), and decrease in serum ET-1 contents (<i>P</i><0.05), and the endomucin fluorescence intensity, SIRT1 and eNOS protein and mRNA were significantly increased (<i>P</i><0.05). Morphological observation revealed that in the model group, light microscope showed incomplete thoracic aorta structure, uneven inner wall, endothelial cell degeneration and swelling, and TEM showed a few swollen mitochondria and autophagy, and a large number of dilated rough endoplasmic reticulum, and these situations were obviously milder in the moxibustion group.</p><p><strong>Conclusions: </strong>Moxibustion can reduce vascular endothelial injury, improve endothelial function and promote endothelial repair in AS mice, which may be related to its function in regulating the SIRT1/eNOS signaling pathway.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"928-935"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on the mechanism of moxibustion in improving autophagy of intestinal epithelial cells in rats with diarrhea-predominant irritable bowel syndrome based on PI3K/AKT/mTOR signaling pathway].","authors":"Ting Wang, Xiao-Ge Song, Jing-Ru Ruan, Xiao-Yu Han, Yu-Qing Wang, Ting-Ting Xiang, Kui-Wu Li, Jing-Wei Zhu, Yu-Cheng Fang, Zi-Ye Wang, Hao-Ran Chu","doi":"10.13702/j.1000-0607.20241113","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20241113","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of moxibustion on visceral hypersensitivity and the phosphatidylinositol-3 kinase (PI3K) /protein kinase B (AKT) /mammalian target of rapamycin (mTOR) signaling pathway in rats with diarrhea-predominant irritable bowel syndrome (IBS-D), so as to explore its mechanisms underlying amelioration of pain reaction of IBS-D by regulating the autophagy level of intestinal epithelial cells and protecting the intestinal mucosal barrier.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Forty male SD rats were randomly divided into control, model, moxibustion and moxibustion+LY294002 groups, with 10 rats in each group. The IBS-D model was established by acetic acid enema + chronic binding method. In the moxibustion group, the rats were given suspended moxibustion on bilateral \"Tianshu\" (ST25) and \"Shangjuxu\" (ST37) for 20 minutes. Rats of the moxibustion+LY294002 group received intraperitoneal injection of PI3K inhibitor LY294002 (0.75 mg/kg) 30 min before each moxibustion intervention. Both groups were treated once a day for 7 days. The minimum colon-rectal water injection volume (minimum water-injection volume) for achieving a score of 3 points of the abdominal withdrawal reflex (AWR) was measured to assess the rats' visceral pain reaction before and after modeling, and after the intervention. H.E. staining was used to observe inflammatory damage of the colonic mucosa. A transmission electron microscopy was employed to examine the mitochondrial structure and the number of autophagosomes in the colon mucosa. The expressions of Occludin, ZO-1, Claudin-2, p62 proteins, and the ratios of p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR and LC3B-Ⅱ/Ⅰ were detected by Western blot. The mRNA expression levels of PI3K, AKT, mTOR, Occludin, ZO-1 and Claudin-2 in the colon tissues were detected by qPCR.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the control group, the minimum water injection volume, and the expression levels of p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR ratios, Occludin, ZO-1 and p62 proteins and the mRNA expression of PI3K, AKT, mTOR, Occludin and ZO-1 were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01), while the protein and mRNA expression level of Claudin-2 and the ratio of LC3B-Ⅱ/Ⅰ were strikingly increased in the model group (&lt;i&gt;P&lt;/i&gt;&lt;0.01). H.E. staining showed disordered arrangement of the intestinal epithelial cells, and infiltration of local inflammatory cells, and electron microscopy observation showed multiple autophagosomes in the model group. In comparison with the model group, the minimum water injection volume, the p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR ratios, the protein expression levels of Occludin, ZO-1 and p62 and the mRNA expressions of PI3K, AKT, mTOR, Occludin and ZO-1 in the colon tissue were considerably increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05, &lt;i&gt;P&lt;/i&gt;&lt;0.01), while the protein and mRNA expression of Claudin-2 and the ratio of LC3B-Ⅱ/Ⅰ were apparently decreased in the moxibustion group (&lt;i&gt;P&lt;/i&gt;&lt;0.01). After administration of PI","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 8","pages":"936-945"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144972271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}