针刺研究最新文献

{"title":"[Electroacupuncture intervention promotes browning of white adipose tissue by regulating LKB1/AMPK pathway in obese mice].","authors":"Ying-Rong Zhang, Yan-Ji Zhang, Wei Huang, Xia Chen, Jia-Jie Wang, Zhong-Yu Zhou","doi":"10.13702/j.1000-0607.20240109","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240109","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) on hypothalamic liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling pathway, sympathetic nerve activity and browning of white adipose tissue (WAT) in obese mice, so as to explore the possible central mechanisms of EA in improving obesity.</p><p><strong>Methods: </strong>C57BL/6J male mice were divided into normal group (<i>n</i>=8) and high fat diet group (<i>n</i>=30) randomly, and fed by normal diet and high fat diet for 8 weeks, respectively. In the high fat diet group, mice with body weight over 20% heavier than that of the normal group were considered as obese mice. The obese mice were divided into model and EA groups (<i>n</i>=8 in each group). Mice in the EA group were treated with EA (2 Hz/15 Hz, 1 mA, 3 mm needling depth) at \"Zusanli\" (ST36) and \"Tianshu\" (ST25) for 30 min, 5 times a week for 4 weeks. The mice's body weight and length were measured for calculating the Lee's index. The contents of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and noradrenaline (NE) were detected by ELISA. The weight of abdominal white adipose tissue was weighed, and the morphological changes of abdominal adipose tissue were observed by HE staining. The mRNA and protein expression levels of LKB1 and AMPK in hypothalamus, peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), uncoupling protein-1 (UCP1), peroxisome proliferator-activated receptor γ (PPAR-γ) and β3-adrenergic receptor (β3-AR) in abdominal white adipose tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively.</p><p><strong>Results: </strong>Compared with the normal group, the body weight, Lee's index, serum contents of TC, TG and LDL-C, abdominal WAT mass, hypothalamus LKB1, AMPK mRNA and protein expression were increased (<i>P</i><0.01), while serum HDL-C and NE contents, mRNA and protein expression of β3-AR, PGC-1α, UCP1 and PPAR-γ in adipose tissue were decreased (<i>P</i><0.01) in the model group. The diameter of adipocytes in abdominal WAT was significantly increased, and the number of cells per unit area was decreased in the model group. Following EA intervention, the body weight, Lee's index, serum contents of TC, TG and LDL-C, abdominal WAT mass, hypothalamus LKB1, AMPK mRNA and protein expression were decreased (<i>P</i><0.01), while the serum HDL-C and NE contents, mRNA and protein expression of β3-AR, PGC-1α, UCP1 and PPAR-γ were increased (<i>P</i><0.01). The diameter of adipocytes in abdominal WAT was decreased and the number of cells per unit area was increased in the EA group.</p><p><strong>Conclusions: </strong>EA can reduce the body weight and Lee's index in obese mice, regulate blood lipids, and reduce the weight of abdominal WAT, which may be closely related to its effect in regulating the hypothalamic LKB1/AMPK signaling pathway, ac","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"393-401"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanisms of acupuncture in inhibition of ferroptosis in intracerebral hemorrhage mice based on RNA m6A methylation sequencing].","authors":"Hao Liu, Jia DU, Chen Ruan, Pei-Pei Feng, Zhong-Wei Guo, Ke-Lang Lou, Xiao-Qing Yu, Xin-Wei Li","doi":"10.13702/j.1000-0607.20240002","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240002","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of acupuncture on N6-methyladenosine (m6A) methylation and ferroptosis in brain tissue of mice with intracerebral hemorrhage (ICH), so as to explore its mechanisms underlying promoting neural function recovery.</p><p><strong>Methods: </strong>Fifty-four male C57BL/6J mice were randomly divided into sham operation, model and acupuncture groups, with 18 mice in each group. The ICH model was established by injection of the mouse's auto-blood (10 μL) into the right caudate nucleus. Mice of the acupuncture group received acupuncture at \"Baihui\" (GV20) for 30 min, twice daily for 7 days. The neurological functions (locomotor and balance deficits) were evaluated according to wire hanging test (0-5 points) and balance walking test (0-5 points) before modeling and after treatment. Histopathological changes in the brain were observed by HE staining. RNA-sequencing and m6A- sequencing were used to analyze the differential m6A modification genes. Immunofluorescence and Western blot were used to detect the expression of glutathione peroxidase 4 (Gpx4) and sirtuin 3 (Sirt3) proteins in brain tissue, respectively.</p><p><strong>Results: </strong>HE staining showed that in the brain tissue nucleus was pyknotic, inflammatory cells were infiltrated, neurons were edematous and necrotic in the model group, which were relatively milder in the acupuncture group. The sequencing results showed that 592 differentially expressed genes were screened in the model group <i>vs</i> sham operation group, and 849 differentially expressed genes were screened in the acupuncture group <i>vs</i> model group. Five genes that inhibit ferroptosis were obtained by intersecting with genes that inhibit ferroptosis, namely charged multi vesicular body protein (Chmp) 1a, Chmp6, glutaredoxin (Glrx) 5, Gpx4, and Sirt3. Compared with the sham operation group, the wire hanging test and balance walking test scores, gene expression levels of Chmp1a, Chmp6, Glrx5, Gpx4, and Sirt3, Gpx4/NeuN and Sirt3/NeuN co-expressed fluorescence intensity, the protein expressions of Gpx4 and Sirt3 in brain tissue were all significantly decreased in the model group (<i>P</i><0.01). Following the interventions, the wire hanging test and balance walking test scores, gene expression levels of Chmp1a, Chmp6, Glrx5, Gpx4, and Sirt3, Gpx4/NeuN and Sirt3/NeuN co-expressed fluorescence intensity, the protein expressions of Gpx4 and Sirt3 in brain tissue were all significantly increased in the acupuncture group compared with the model group (<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Acupuncture can ameliorate neurological deficits, which may be associated with its function in improving m6A RNA modification and RNA expression in mice with ICH, and promoting the expression of ferroptosis antioxidation related proteins Gpx4 and Sirt3 in brain tissue.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"366-374"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of acupotomy on lipid peroxidation of chondrocytes in rabbits with knee osteoarthritis].","authors":"Xin Qi, You-Nan Chen, Yun-Xuan Ma, Long-Fei Xing, Yong-Qi Cui, Chang-Qing Guo","doi":"10.13702/j.1000-0607.20240323","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240323","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of acupotomy on expression of long-chain acyl-CoA synthetase 4 (ACSL4), lysophosphatidylcholine acyltransferase 3 (LPCAT3), malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) in knee cartilage of rabbits with knee osteoarthritis (KOA), so as to explore whether acupotomy can treat KOA by inhibiting lipid peroxidation of chondrocytes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Six-month-old New Zealand male rabbits were randomly divided into control, model, acupotomy and electroacupuncture (EA) groups, with 7 rabbits in each group. The KOA model was established by left hindlimb straightening fixation according to Videman method. In the acupotomy group, the high stress points, such as tendons attachment points of the vastus medialis, vastus lateralis, rectus femoris, biceps femoris and pes anserine bursa of the left hind limb knee joint were selected for acupotomy lysis treatment, once a week for 3 weeks. In the EA group, EA (2 Hz/100 Hz, 3 mA) was applied to \"Neixiyan\" (EX-LE4), \"Dubi\" (ST35), \"Xuehai\" (SP10) and \"Liangqiu\" (ST34) of the left hind limb of rabbits for 20 min, once every other day for 3 weeks. Before and after the treatment, Lequesne MG score and passive range of motion (PROM) of knee joint were detected in each group. After the treatment, safranin O-fast green staining was used to observe the morphological changes of rabbit knee cartilage and Mankin scoring was evaluated. Immunohistochemistry method was used to detect the location and positive expression of ACSL4. Western blot was used to detect the protein expression levels of ACSL4 and LPCAT3, and TBA and ELISA methods were used to detect the expression levels of MDA and 4-HNE in the affected knee cartilage, respectively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared to the control group, the Lequesne MG score was significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.01), while PROM was significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the model group;after the intervention and compared to the control group, the model group exhibited significant increase in Mankin score, enhancement in the positive expression of ACSL4 in knee cartilage (&lt;i&gt;P&lt;/i&gt;&lt;0.01), along with significant elevation in ACSL4 and LPCAT3 protein expression levels (&lt;i&gt;P&lt;/i&gt;&lt;0.01), as well as elevated contents of MDA and 4-HNE (&lt;i&gt;P&lt;/i&gt;&lt;0.01). Compared to the model group, both the acupotomy group and EA group showed significant reductions in Lequesne MG score (&lt;i&gt;P&lt;/i&gt;&lt;0.01) and increases in PROM (&lt;i&gt;P&lt;/i&gt;&lt;0.01). Additionally, there were marked decreases in Mankin score and the positive expression of ACSL4 in knee cartilage (&lt;i&gt;P&lt;/i&gt;&lt;0.01), as well as significant decreases in the protein expression levels of ACSL4 and LPCAT3 (&lt;i&gt;P&lt;/i&gt;&lt;0.01). Moreover, the contents of MDA and 4-HNE decreased significantly (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared to the EA group, the acupotomy group exhibited reduced Lequesne MG score (&lt;i&gt;P&lt;/i&gt;&lt;0.01), increased PROM (&lt;i&gt;P&lt;/i&gt;&lt;0.01), significant reductions in ACSL4 and LPC","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"402-410"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of moxibustion on lipid peroxidation in the spinal cord of rats with urinary retention due to spinal cord injury].","authors":"Chen-Xu Qi, Kai-Yan DU, Jing-Yu Cao, Hui-Xin Wang, Shu-Ting Duan, Jing Gao, Cheng-Mei Liu","doi":"10.13702/j.1000-0607.20240148","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240148","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of moxibustion on urinary dynamics and lipid oxidation in spinal cord tissue of rats with urinary retention after spinal cord injury (SCI), so as to explore its possible mechanism in improving urinary retention after SCI.</p><p><strong>Methods: </strong>Female Wistar rats were randomly divided into sham operation, model and moxibustion groups, with 10 rats in each group. SCI induced urinary retention model was established by using Allen's method. Moxibustion was applied to \"Shenque\" (CV8), \"Guanyuan\"(CV4) and \"Zhongji\" (CV3) for 20 min, once daily for 14 d. The maximum bladder volume, bladder leakage point pressure and bladder compliance of rats were measured by urodynamics. The changes of spinal cord histomorphology were observed by H.E. staining. The ultrastructure of neurons in spinal cord was observed by transmission electron microscope. Serum reactive oxygen species (ROS) content was detected by ELISA. Serum glutathione (GSH) and malondialdehyde (MDA) contents were detected by colorimetry. The protein expression levels of lipid oxidation-associated protein esteryl-CoA synthetase long chain family member 4 (ACSL4), lyso phosphatidyl cholin acyl transferase 3 (LPCAT3) and lipoxygenase (LOX) in spinal cord were detected by Western blot.</p><p><strong>Results: </strong>Compared with sham operation group, the maximum bladder volume, bladder compliance, bladder leakage point pressure, serum ROS and MDA contents, the protein expression levels of ACSL4, LPCAT3 and LOX in spinal cord tissue were increased (<i>P</i><0.01, <i>P</i><0.05) in the model group, while the GSH content in serum was decreased (<i>P</i><0.01). After the treatment and compared with model group, the maximum bladder capacity, bladder compliance, bladder leakage point pressure, serum ROS and MDA contents, the protein expression levels of ACSL4, LPCAT3 and LOX in spinal cord tissue in the moxibustion group were decreased (<i>P</i><0.01, <i>P</i><0.05), while the GSH content in serum was increased (<i>P</i><0.05). H.E. staining indicated morphological damage in the spinal cord of rats in the model group, with disappeared normal neurons, which were relatively milder in the moxibustion group.</p><p><strong>Conclusions: </strong>Moxibustion can improve the bladder function of rats with urinary retention after SCI, which may be associated with its functions in down-regulating lipid oxidation in spinal cord tissue, reshaping spinal cord function.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"419-425"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical efficacy and safety of acupuncture therapy for central facial palsy after stroke: a systematic review and Meta-analysis].","authors":"Xi-Rui Wang, Yuan-Yuan Wu, Yue-Rong Chen, Si-Yuan Tong, Jun-Yi Wang, Zong-Lin Wu, Jin-Zhu Gao","doi":"10.13702/j.1000-0607.20240426","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240426","url":null,"abstract":"<p><strong>Objectives: </strong>To systematically evaluate the efficacy and safety of acupuncture therapy in the treatment of central facial palsy after stroke.</p><p><strong>Methods: </strong>A computerized search of articles on acupuncture treatment of central facial palsy was conducted from databases of China National Knowledge Infrastructure(CNKI), Wanfang Data Knowledge Service Platform (Wanfang), VIP Information Chinese Journal Service Platform (VIP) , China Biomedical Literature Database(CBM), Cochrane Library, PubMed, Embase and Web of Science from their inception to September 25^th, 2023. The Cochrane risk of bias assessment tool was used to evaluate the risk of bias of all the included literature, and Meta-analysis on the extracted data was conducted using Review Manager 5.4 software.</p><p><strong>Results: </strong>Eleven studies with a total of 893 patients were finally included, and the bias risks of the included studies were all \"some concerns\". The results of Meta-analysis suggested that:1) the observation group was superior to the control group in improving the the House-Brackmann facial nerve grading system (H-B) scores (<i>MD</i>=6.41, 95%<i>CI</i> [3.69, 9.14], <i>Z</i>=4.62, <i>P</i><0.000 01), facial disability index (FDI) scores (somatic function) (<i>MD</i>=2.72, 95%<i>CI</i> [0.31, 5.12], <i>Z</i>=2.21, <i>P</i>=0.03), and clinical effective rate (<i>RR</i>=1.25, 95%<i>CI</i> [1.15, 1.35], <i>Z</i>=5.57, <i>P</i><0.000 01);2) the observation group was not significantly different from the control group in improving the Toronto facial nerve assessment system (TFGS) scores (<i>MD</i>=3.66, 95%<i>CI</i> [ -2.33, 9.65], <i>Z</i>=1.20, <i>P</i>=0.23), the degrees of facial nerve paralysis (DFNP) scores (<i>MD</i>=4.60, 95%<i>CI</i> [ -1.14, 10.34], <i>Z</i>=1.57, <i>P</i>=0.12), FDI scores (social function) (<i>MD</i>=3.00, 95%<i>CI</i> [ -0.67, 6.66], <i>Z</i>=1.60, <i>P</i>=0.11).</p><p><strong>Conclusions: </strong>Acupuncture therapy has advantages in clinical efficacy and safety than conventional drugs and other sports rehabilitation therapies in improving post-stroke central facial palsy. However, more high-quality clinical trials are warranted to provide adequate clinical evidence.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"467-476"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of electroacupuncture on cortactin and cytoskeletal stability in lung tissue of mice with chronic obstructive pulmonary disease].","authors":"Zhu Pan, Hui-Hui Wu, Hai-Yan Liu, Xin-Fang Zhang, Yuan Wang, Shu-Wen Xu, Yu-Jie Zhang, Wen-Mei Liu, Zi-Bing Liu","doi":"10.13702/j.1000-0607.20240044","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240044","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effects of electroacupuncture (EA) at \"Zusanli\" (ST36) and \"Feishu\" (BL13) points on Cortactin and cytoskeletal stability of pulmonary endothelial cells in mice with chronic obstructive pulmonary disease (COPD), and to explore the mechanism by which EA alleviates inflammatory damage in COPD.</p><p><strong>Methods: </strong>The C57BL/6 mice were randomly divided into 5 groups:normal group, model group, EA group, Cortactin small interfering RNA (si-Cortactin) group, and si-Cortactin + EA group, with 10 mice in each group. A COPD model was established through 12 weeks of cigarette exposure. Nasal instillation of Cortactin siRNA was performed 24 h before EA treatment. EA at bilateral ST36 and BL13 with each EA session lasted for 30 min, conducted once every other day for a continuous period of 2 weeks. Pulmonary function was assessed using a small animal lung function analyzer and HE staining was used to observe lung histopathology. ELISA was employed to measure the contents of tumor necrosis factor-α (TNF-α) and Caspase-3 in bronchoalveolar lavage fluid (BALF) and serum. TUNEL assay was used to detect apoptosis of pulmonary endothelial cells, and double immunofluorescence staining was performed to assess cytoskeletal stability in pulmonary endothelial cells. The expression levels of Caspase-3 and Cortactin mRNA in lung tissue were evaluated using qPCR, while Western blot was used to measure the protein expression levels of Caspase-3 and Cortactin in lung tissues.</p><p><strong>Results: </strong>Compared to the normal group, the model group exhibited decreases in forced vital capacity (FVC), forced expiratory volume in 0.05 s (FEV0.05), forced expiratory volume in 0.1 s (FEV0.1), FEV0.05/FVC, and FEV0.1/FVC (<i>P</i><0.001). Lung tissue showed severe inflammatory infiltration, accompanied by increased contents of TNF-α and Caspase-3 in serum and BALF (<i>P</i><0.001). The expression levels of Caspase-3 mRNA and protein were elevated, while Cortactin mRNA and protein levels were reduced in lung tissue (<i>P</i><0.001). There was significant apoptosis of pulmonary endothelial cells and disruption of the cytoskeletal structure (<i>P</i><0.001). After treatment, the EA group demonstrated marked improvement in these parameters compared to the model group (<i>P</i><0.001, <i>P</i><0.01, <i>P</i><0.05). Following intervention with si-Cortactin, the indicators in the si-Cortactin group worsened further (<i>P</i><0.01, <i>P</i><0.001, <i>P</i><0.05). The si-Cortactin + EA group showed improvements in these parameters relative to the si-Cortactin group (<i>P</i><0.001, <i>P</i><0.05, <i>P</i><0.01).</p><p><strong>Conclusions: </strong>EA can alleviate inflammatory pathological damage in the lung tissues of mice with COPD, potentially through up-regulating Cortactin, stabilizing the cytoskeleton of pulmonary endothelial cells, and inhibiting apoptosis.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"384-392"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture improves synaptic plasticity and cognitive dysfunction via down-regulating HDAC3 in mice of Alzheimer's disease].","authors":"Lan-Feng Lai, Xin Li, Hong-Zhu Li, Zhi-Min Li, Lian Liu, Yu-Ying Zhang, Han Yang, Bing-Yi Luo, Wei Yi, Neng-Gui Xu, Jia-Ying Zhao","doi":"10.13702/j.1000-0607.20240214","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240214","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture(EA) on histone deacetylase 3 (HDAC3), synaptic plasticity and N-methyl-D-aspartate (NMDA) receptors in the hippocampus of mice with Alzheimer's disease(AD), so as to explore the underlying mechanism of EA in treatment of AD.</p><p><strong>Methods: </strong>5XFAD mice were randomly divided into EA group, model group and sham-acupuncture group, with 13 mice in both the EA group and the model group, and 7 mice in the sham-acupuncture group. Thirteen wild-type mice from the same litter were taken as the normal control group. The mice in the EA group received EA at \"Baihui\" (GV20)and \"Dazhui\" (GV14) for 15 min once daily, 6 times a week for 4 weeks. The mice in the sham-acupuncture group received sham EA, i.e., the needle was inserted into the rubber clay which was placed on the surface of the corresponding acupoints. The novel object recognition(NOR), Y-maze and Morris water maze(MWM) tests were used to observe the cognitive functions of mice. Electrophysiological technique was used to detect long-term potentiation (LTP) of the hippocampal neurons and Western blot was used to detect the relative expressions of HDAC3 and NMDAR-related receptors (NMDAR1, NMDAR2A, NMDAR2B) in the hippocampus.</p><p><strong>Results: </strong>Compared with the normal control group, 5XFAD mice in the model group showed decreased(<i>P</i><0.01, <i>P</i><0.05) preference index for new object recognition, alternative arm ratio (AAR), number of times crossing the original platform, percentage of time and distance traveled in the target quadrant, NMDAR2B, NMDAR2A and NMDAR1 protein expression levels, with prolonged(<i>P</i><0.01) escape latency, and increased (<i>P</i><0.05) protein relative expression of HDAC3. At the same time, with high-frequency stimulation, the slope of fEPSP was decreased(<i>P</i><0.01, <i>P</i><0.05)in the 5XFAD mice. After EA intervention, comparison between the EA and the model groups revealed that, the preference index for new object recognition, AAR were increased (<i>P</i><0.01, <i>P</i><0.05) in the EA group, the escape latency was shortened (<i>P</i><0.05), and the number of times crossing the platform, percentage of time and distance traveled in the target quadrant, the slope of fEPSP, and the protein relative expressions of NMDAR2B, NMDAR2A and NMDAR1 in the hippocampus were increased (<i>P</i><0.01, <i>P</i><0.05), while the protein relative expression of HDAC3 decreased (<i>P</i><0.01). Compared with sham-acupuncture group, the above indexes improved to different degree in the EA group (<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>EA of GV20 and GV14 can restore the impaired LTP and improve the cognitive impairment, which may be related to increasing the expressions of NMDA-related receptor proteins and down-regulating the expression of HDAC3 in the hippocampus of 5XFAD mice.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"375-383"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on moxibustion therapy for improving chemotherapy-induced bone marrow suppression].","authors":"Zun-Yuan Li, Mei-Hong Luo, Ye-Ran Mao, Zhi-Dan Liu","doi":"10.13702/j.1000-0607.20231009","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20231009","url":null,"abstract":"<p><p>Moxibustion therapy is an important traditional non-pharmacological treatment in traditional medicine for improving chemotherapy-induced bone marrow suppression. By reviewing recent studies on moxibustion intervention for chemotherapy-induced bone marrow suppression, this article summarized and analyzed the current research status. In clinical studies, moxibustion therapy that tonifies the spleen, nourishes the kidneys, warms yang, and nourishes blood has been verified to be effective for chemotherapy-induced bone marrow suppression, but the efficacy may vary among individuals receiving different chemotherapy regimens. Experimental studies have shown that moxibustion therapy primarily improves chemotherapy-induced bone marrow suppression by repairing bone marrow tissue structure, increasing the amounts of hematopoietic stem cells, improving bone marrow hematopoietic microenvironment, repairing bone marrow cell DNA, and regulating signaling pathways such as Notch, Wnt, phosphatidylinositol 3-kinase/protein kinase B/ mammalian target protein of rapamycin and other signaling pathways. Future research can further systematically reveal the mechanisms of moxibustion therapy, such as alleviating hematopoietic stem cell aging induced by chemotherapy, regulating miRNAs to improve bone marrow suppression, and investigate the sensitivity of patients with bone marrow suppression caused by different chemotherapy regimens to moxibustion therapy, in order to complete and standardize the application protocols of moxibustion.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"448-458"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The long-term follow-up clinical research of ultrasound-guided warm needle knife in treatment of advanced knee osteoarthritis].","authors":"Jing Yin, Shi-Ru Ju, Xiao-Juan Luo, Yi-Xuan Duan, Zhao-Qing Zhang, Hui-Jun Chen","doi":"10.13702/j.1000-0607.20231059","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20231059","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the long-term efficacy and safety of ultrasound-guided warm needle knife therapy for patients with advanced knee osteoarthritis (KOA).</p><p><strong>Methods: </strong>A total of 70 advanced KOA patients were recruited from the Department of Pain and Rehabilitation of the Third Hospital of Wuhan from June 2020 to June 2022. They were randomized to a treatment group or a control group in a 1∶1 ratio. Patients in the treatment group received ultrasound guided warm needle knife treatment, while patients in the control group received ultrasound guided radiofrequency treatment of the knee sensory plexus, both for 1 time. The primary outcome was the changes in Western Ontario McMaster University Osteoarthritis Index (WOMAC) scores. Other outcomes included the Visual Analogue Pain Scale (VAS) scores, value of Young's modulus of A-shi points, proportion of patients achieving improvement, and adverse events related to study interventions.</p><p><strong>Results: </strong>Compared with baseline, the WOMAC and VAS scores in the two groups were significantly decreased at each timepoints(<i>P</i><0.05), and the value of Young's modulus was significantly decreased at 12 months after treatment. Compared with the control group, the treatment group reported a significantly greater reduction in WOMAC and VAS scores at 6 months and 12 months after treatment(<i>P</i><0.05). The proportion of patients achieving improvement in the treatment group were significantly higher than the control group at 12 months after treatment(<i>P</i><0.05). The value of Young's modulus in the treatment group was significantly lower than the control group at 12 months after treatment(<i>P</i><0.05). No adverse events were reported.</p><p><strong>Conclusions: </strong>Ultrasound guided warm needle knife is effective in improving the function of knee joint and relieving pain in patients with advanced KOA. Further, the long-term efficacy of ultrasound guided warm needle knife for advanced KOA was superior to the ultrasound guided radiofrequency therapy.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"433-438"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on the \"neuro-endocrine-immune\" regulatory mechanism of acupuncture in the treatment of simple obesity].","authors":"Wen-Ting Xie, Yu-Juan Ye, Cui Ma, Yu-Ting Wei, Xing-Ke Yan","doi":"10.13702/j.1000-0607.20231051","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20231051","url":null,"abstract":"<p><p>Acupuncture has a definite effect on the treatment of simple obesity. Acupuncture can effectively reduce the weight of patients and improve the body metabolism. Studies have shown that acupuncture in the treatment of simple obesity is closely related to the regulation of neuro-endocrine-immune function. This article reviews the neuro-endocrine-immune regulatory mechanism of acupuncture in the treatment of simple obesity from the aspects of regulating key nuclei of the central nervous system, improving insulin and leptin resistance, regulating glucose and lipid metabolism, intestinal flora disorders, and regulating related immune inflammatory molecules and inflammatory signaling pathways. This review aims to provide a basis for the mechanism and application research of acupuncture in the treatment of simple obesity.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 4","pages":"459-466"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}