针刺研究最新文献

{"title":"[Effect of \"Tongdu Qishen\" acupuncture on pyroptosis in hippocampus and cortex of SAMP8 mice].","authors":"Meng Wu, Xin Hao, Ting Li, Jun-Jian Tian, Xiao-Ming Gao, Zhi-Gang Li","doi":"10.13702/j.1000-0607.20240754","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240754","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the underlying mechanism of \"Tongdu Qishen\" acupuncture (dredging the Govern Vessel and normalizing mental activities) in treating SAMP8 mice from the perspective of pyroptosis.</p><p><strong>Methods: </strong>Six-month-old male SAMP8 mice were randomly divided into model and \"Tongdu Qishen\" acupuncture (EA) groups, with 12 mice in each group. SAMR1 mice of the same age (<i>n</i>=12) were used as the normal group. In the EA group, EA (2 Hz/50 Hz, 20 min) was applied to \"Baihui\" (GV20) and \"Yintang\" (GV24⁺), and pricking bleeding was applied to \"Shuigou\" (GV26). The treatment was performed once a day, for a total of 28 days. Morris water maze test was used to evaluate the learning and memory ability of mice, and HE staining was used to observe the morphology of cells in the hippocampus and cortex. Nissl staining was used to observe the number of neurons in the hippocampus and cortex. Immunohistochemistry was used to observe the expressions of Aβ_1-40, interleukin (IL)-1β and IL-18 in the hippocampus and cortex. The co-staining of TUNEL and Caspase-1 was detected by immunofluorescence. Western blot and real-time quantitative PCR were used to observe the protein and mRNA expression levels of Nod-like receptor 3 (NLRP3), Caspase-1 and Gasdermin D (GSDMD) in the hippocampus and cortex, respectively.</p><p><strong>Results: </strong>Behavioral results showed that compared with the normal group, the escape latency was prolonged (<i>P</i><0.001), the dwell time in the original platform quadrant and the times of crossing the original platform were considerably decreased (<i>P</i><0.001) in the model group. In comparison with the model group, the escape latency was shortened (<i>P<</i>0.05, <i>P<</i>0.001, <i>P<</i>0.01), and the dwell time in the original platform quadrant was significantly increased (<i>P<</i>0.01) in the EA group. Following modeling, the number of neurons was decreased (<i>P</i><0.001), the positive expression of Aβ_1-40, IL-1β and IL-18 in the hippocampus and cortex, the co-expression of TUNEL and Caspase-1, and the protein and mRNA expression levels of NLRP3, Caspase-1, GSDMD were all increased (<i>P</i><0.001) in the model group relevant to the normal group. After EA intervention, modeling induced increase and decrease of indexes mentioned above were completely reversed (<i>P<</i>0.01, <i>P<</i>0.05, <i>P<</i>0.001).</p><p><strong>Conclusions: </strong>\"Tongdu Qishen\" acupuncture may improve the cognitive function of SAMP8 mice by regulating the NLRP3/Caspase-1/GSDMD pathway, relieving the release of inflammatory factors and reducing the content of Aβ_1-40.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"995-1004"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of acupuncture on the efferocytosis pathway of Mer receptor tyrosine kinase in mice with adjuvant arthritis].","authors":"Ji-Yu Zhao, Gong-Ming Yuan, Pei-Yun Li, Xin-Meng Wang, Zi-Xuan Liu, Yuan Xu, Yong-Ming Guo, Yuan Li","doi":"10.13702/j.1000-0607.20240585","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240585","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of acupuncture at \"Zusanli\" (ST36) on the expression of Mer receptor tyrosine kinase (MerTK)-related efferocytosis regulatory molecules in mice with adjuvant arthritis induced by complete Freund's adjuvant (CFA), so as to explore the potential mechanism of acupuncture on efferocytosis in adjuvant arthritis.</p><p><strong>Methods: </strong>C57BL/6J mice were randomly divided into a saline group, a model group, and an acupuncture group (10 mice/group). The right hind footpad of mice in the saline group was injected with 0.9% sodium chloride solution, while those in the model group and the acupuncture group were injected with CFA (50 μL) to establish the model of adjuvant arthritis. Mice in the acupuncture group received acupuncture at bilateral ST36 once a day for a total of 7 times, and mice in the saline group and the model group were fixed in the same way. During the experiment, paw swelling degree and the paw withdrawal thermal latency (PWTL) of mice were measured before modeling, on the day of modeling (D0) and on the 1^st, 3^rd, 5^th and 7^th day of acupuncture (D1, D3, D5, D7) to evaluate paw swelling and pain conditions. The mRNA expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β in spinal cord tissue were detected by real-time quantitative PCR. The protein expressions of MerTK, phosphorylated (p)-signal transducer and activator of transcription 3 (STAT3), STAT3, and programmed death-ligand 1 (PD-L1) in footpad and spinal cord tissue were detected by Western blot.</p><p><strong>Results: </strong>After modeling, compared with the saline group, the PWTL on D0-D7, the protein expression of PD-L1 in spinal cord and protein expressions of MerTK and PD-L1 in footpad, the phosphorylation level of STAT3 in spinal cord and footpad were decreased (<i>P</i><0.01) in the model group, while the degree of paw swelling, mRNA expressions of TNF-α, IL-6, and IL-1β in spinal cord tissue were increased (<i>P</i><0.01, <i>P</i><0.05). Compared with the model group, the PWTL on D1-D7, the protein expressions of MerTK, PD-L1 and phosphorylation level of STAT3 in spinal cord and footpad were increased (<i>P</i><0.01) in the acupuncture group after acupuncture intervention, while the degree of paw swelling on D7, the mRNA expressions of TNF-α, IL-6, and IL-1β in spinal cord tissue were significantly decreased(<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Acupuncture can improve the symptoms of mice with adjuvant arthritis, which may be related to promoting MerTK-mediated efferocytosis and thus exerting an anti-inflammatory effect.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1021-1028"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of electroacupuncture on E/I balance of prefrontal cortex and hippocampal pyramidal neurons in anxiety-like mice].","authors":"Zeng-Ming Ma, Zhi-Yuan Huang, Jin-Dong Chen, Yu-Ping Xia, Chuan-An Zhu","doi":"10.13702/j.1000-0607.20240586","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240586","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) on the excitatory/inhibitory (E/I) balance of prefrontal cortex and hippocampal pyramidal neurons in anxiety-like mice induced by chronic unpredictable mild stress (CUMS).</p><p><strong>Methods: </strong>Forty-eight male C57BL/6 mice were randomly divided into control, control+EA (CE), model, and model+EA (ME) groups, with 12 mice in each group. The anxiety model was established by using CUMS (restraint, day-night reversal, forced swimming in warm water, food/water deprivation for 24 h, rearing in a humid environment, and shaking the cage) for 21 consecutive days. EA (2 Hz, 1 mA) was applied to \"Baihui\" (GV20) and \"Yintang\" (GV24⁺) for 30 min, once daily for 10 consecutive days. The anxiety-like behaviors of mice were evaluated by open field test and elevated plus maze (EPM) test. The E/I balance of the prefrontal cortex and hippocampal pyramidal neurons of the mice were observed by whole-cell recording.</p><p><strong>Results: </strong>After modeling, the exploration time in the central area of open field test, the times of entries and residence time in the open arm of EPM test were significantly reduced (<i>P</i><0.01) in the model group, while the spontaneous excitatory postsynaptic current (sEPSC) frequency, capacitance and E/I ratio of sEPSC in the dorsolateral prefrontal cortex (dlPFC), medial prefrontal cortex (mPFC) and ventral hippocampal CA1 (vCA1) were increased significantly (<i>P</i><0.01). Compared with the model group, the exploration time in the central area of the open field, the times of entries and residence time in the open arm of EPM test were significantly increased (<i>P</i><0.05, <i>P</i><0.01), while the frequency, capacitance and E/I ratio of sEPSC in the dlPFC, mPFC and vCA1 were significantly decreased (<i>P</i><0.01, <i>P</i><0.05) in the ME group. In comparison with the CE group, the exploration time in the central area of the open field, the times of entries and residence time in the open arm of EPM test were significantly reduced (<i>P</i><0.01), while the frequency, capacitance and E/I ratio of sEPSC in the dlPFC, mPFC and vCA1 were significantly increased (<i>P</i><0.01, <i>P</i><0.05) in the ME group.</p><p><strong>Conclusions: </strong>Chronic stress may induce anxiety in mice by increasing the E/I of pyramidal neurons in dlPFC, mPFC and vCA1. EA may improve anxiety-like behaviors in mice by regulating the E/I balance of the above brain regions.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1055-1063"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Blood-letting plus cupping relieves endotoxin-induced fever by improving hypothalamic mitochondrial metabolism in rabbits].","authors":"Xin Wang, Wen-Yu Liu, Ying Wang, Jin Wu, Bo-Wen Xiang, Ying Pei","doi":"10.13702/j.1000-0607.20240700","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240700","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of blood-letting plus cupping on endotoxin-induced fever in rabbits and to explore its central antipyretic mechanism based on mitochondrial energy metabolism.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Seventy-two male Japanese white rabbits were randomly divided into six groups:blank control, model, medication, blood-letting, cupping, and blood-letting cupping groups, with 12 rabbits in each group. The fever model was established by injection of endotoxin (200 ng/kg) into the ear vein. One hour after modeling, the rabbits in the medication group received gavage of indomethacin, while the other groups were given the same volume of physiological saline via gavage. The rabbits in the blood-letting group received blood-letting at the \"Dazhui\" (GV14) acupoint, those of the cupping group received cupping at the GV14 area with -0.02 MPa pressure for 15 min, and those of the blood-letting cupping group first underwent blood-letting at GV14 followed by cupping with the same parameters as those of the simple blood-letting and simple cupping groups. Temperature changes in each group were recorded at 1, 2, 3, 4, 5 and 6 h after modeling. A transmission electron microscopy was used to observe the ultrastructure of hypothalamic mitochondria, the ELISA was employed to measure the contents of serum IL-1β and IL-6. An Oxygraph-2k was used to assess the mitochondrial respiratory function, the ATP content was determined with an ATP assay kit, and the expression levels of uncoupling protein 2 (UCP2) and mitofusin 2 (Mfn2) in the hypothalamic tissue were measured using the WES (automatic Western blot quantitative analysis system).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the blank control group, the model group showed a significant increase in the body temperature from 1 h on after modeling (&lt;i&gt;P&lt;/i&gt;&lt;0.01), contents of serum IL-1β and IL-6 (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and the expression of hypothalamic UCP2 protein (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and a significant decrease in the mitochondrial oxygen consumption (&lt;i&gt;P&lt;/i&gt;&lt;0.05) and ATP content (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and the expression of Mfn2 protein in the hypothalamic tissue (&lt;i&gt;P&lt;/i&gt;&lt;0.01). Compared to the corresponding time points of the model group, the body temperature at 2, 3, 4, 5 and 6 h in both medication and blood-letting cupping groups, at 2, 3, 4 and 6 h in both cupping and blood-letting groups were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05). No significant differences were found between the medication and blood-letting cupping groups, and between the cupping and blood-letting groups in lowering the body temperature at the five time points. The effects of simple cupping at 2, 3, 5 and 6 h and simple blood-letting at 2, 3, 4, 5 and 6 h were respectively obviously weaker than those of the medication in lowering body temperature at the corresponding time points (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05). The effects of simple cupping at 5 and 6 h and simple blood-letting at 2, 3, 5 and 6 h w","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1072-1080"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of transcutaneous electrical acupoint stimulation at Neiguan (PC6) and Jianshi (PC5) on autonomic nervous function and inflammatory factors in frail elderly patients after surgery].","authors":"Meng Zhang, Nan Zhao, Ge Yang, Huan-Huan Zhang, Jian-Li Li","doi":"10.13702/j.1000-0607.20241107","DOIUrl":"10.13702/j.1000-0607.20241107","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the effects of transcutaneous electrical acupoint stimulation (TEAS) at the bilateral acupoints Neiguan (PC6) and Jianshi (PC5) on heart rate variability (HRV) and inflammatory factors in frail elderly patients undergoing laparoscopic colorectal cancer surgery under general anesthesia.</p><p><strong>Methods: </strong>A total of 78 frail elderly patients undergoing elective laparoscopic colorectal cancer surgery were randomized into the TEAS group (39 patients, with 2 patients dropping out) and the control group (39 patients, with 1 patient dropping out). In the TEAS group, TEAS was applied to the bilateral PC6 and PC5 from 30 min before anesthesia induction until the end of the surgery. The control group was connected to an electronic acupuncture instrument at the same acupoints but did not receive electrical stimulation. A short-term Holter electrocardiogram was used to collect HRV parameters in the frequency domain, including low-frequency power (LF), high-frequency power (HF), and the LF/HF ratio. Serum concentrations of C-reactive protein (CRP) and interleukin-6 (IL-6) were measured using ELISA. The Quality of Recovery-15 (Qor-15) score was recorded, along with adverse reactions such as postoperative nausea and vomiting (PONV), palpitations, chest tightness, and chest pain.</p><p><strong>Results: </strong>Compared with 1 d before surgery, LF and HF were significantly decreased (<i>P</i><0.05), and the LF/HF ratio significantly increased (<i>P</i><0.05) at 1, 2, and 3 d after surgery in the control group. In the TEAS group, both LF and the LF/HF ratio were significantly decreased (<i>P</i><0.05) at 1, 2, and 3 d after surgery. Compared with the control group, LF and HF levels were significantly increased (<i>P</i><0.05), and the LF/HF ratio was significantly decreased (<i>P</i><0.05) in the TEAS group at 1, 2, and 3 d after surgery. Both groups exhibited increased levels of CRP and IL-6 at 1, 3, and 5 d after surgery compared to 1 d before surgery (<i>P</i><0.05), with the TEAS group showing lower CRP and IL-6 levels than the control group (<i>P</i><0.05). Compared to baseline before surgery, the Qor-15 scores of both groups were decreased at 1, 2, and 3 d after surgery (<i>P</i><0.05), with the TEAS group showing significantly higher Qor-15 scores than those in the control group (<i>P</i><0.05). The incidence of PONV, palpitations, chest tightness, and chest pain in the TEAS group was lower than that in the control group (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>TEAS at the PC6 and PC5 can regulate autonomic nervous function, reduce the early postoperative sympathetic nerve excitation, maintain parasympathetic nerve tension, reduce inflammatory responses, improve the quality of postoperative recovery, and decrease the incidence of postoperative adverse reactions in frail elderly patients after laparoscopic colorectal cancer surgery.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 7","pages":"815-821"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of moxibustion on the cGAS/STING signaling pathway in synovial tissue of rats with rheumatoid arthritis].","authors":"Chuan-Yu Peng, Rong-Lin Cai, Feng Hao, Lei Liu, Chuan-Ying Zhan, Lu He, Ming-Yang Jin","doi":"10.13702/j.1000-0607.20240487","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240487","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of moxibustion on the cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) signaling pathway in the synovial tissue of knee joints in rheumatoid arthritis (RA) model rats, and to explore the mechanism by which moxibustion alleviates synovial inflammatory injury.</p><p><strong>Methods: </strong>Forty male SD rats were randomly divided into 4 groups (10 rats in each group):blank, model, moxibustion, and triptolide groups. The RA model was induced by injecting complete Freund's adjuvant into the right hind toe combined with exposure to wind, cold, and damp environments. The moxibustion group received daily 20 min treatment at \"Zusanli\" (ST36) or \"Shenshu\" (BL23) for 15 d;the triptolide group was administered triptolide suspension (8 mg/kg) via gavage for 15 d. The joint swelling degree of right hind toe was measured by water displacement;the synovial morphology was assessed via HE staining;the mitochondrial ultrastructure was observed by transmission electron microscopy;the synovial protein expression levels of cGAS, STING, TANK-binding kinase 1 (TBK1), phosphorylated interferon regulatory factor 3 (p-IRF3) and phosphorylated nuclear factor-kappa B p65(p-NF-κB p65) were detected by Western blot;serum contents of interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), C-X-C motif chemokine ligand 10 (CXCL10), and interferon-β (IFN-β) were measured by ELISA.</p><p><strong>Results: </strong>Compared with the blank group, the model group showed increased joint swelling degree (<i>P</i><0.01), irregular synovial lining cell distribution, marked hyperplasia, and inflammatory cell infiltration;synovial cGAS, STING, TBK1, p-IRF3, and p-NF-κB p65 protein expression levels and serum IL-6, ICAM-1, CXCL10, and IFN-β contents were significantly elevated (<i>P</i><0.01). Compared with the model group, both moxibustion and triptolide interventions reduced joint swelling degree (<i>P</i><0.01), improved synovial histopathology, and down regulated the aforementioned proteins and cytokines (<i>P</i><0.01). No significant differences were observed between the moxibustion and triptolide groups.</p><p><strong>Conclusions: </strong>Moxibustion attenuates joint swelling, inflammatory cytokine release, and synovial injury in RA model rats, potentially via suppression of the cGAS/STING pathway.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1029-1036"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism research progress of acupuncture for ameliorating pain-related emotions through regulating GABAergic system].","authors":"Hong-Yu Lu, Bao-Jin Wang, Cui Ma, Xing-Ke Yan","doi":"10.13702/j.1000-0607.20240666","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240666","url":null,"abstract":"<p><p>Pain-related emotions refer to the negative emotions such as depression and anxiety triggered by pain, which seriously impact patients' physical and mental health as well as the quality of life. The gamma-aminobutyric acid (GABA) ergic system is the primary inhibitory neurotransmitter system in the brain and plays a significant role in the onset and development of pain-related emotions. Acupuncture can effectively ameliorate the negative emotions such as depression and anxiety caused by pain. Its mechanism is closely related to the regulatory effect of acupuncture on GABAergic system. Acupuncture is active in modulating GABAergic neurons, up-regulating the expression of GABAergic neuron subtype parvalbumin neurons and down-regulating the expression of GABAergic neuron subtype neuropeptide Y; up-regulating the mRNA expression of GABAA receptor (GABAAR) and the protein expression of GABAB2 in the amygdala, and down-regulating the protein expression of GABAAR in the anteiror cingulate cortex. Besides, acupuncture can regulate the expressions of the key factors such as gutamate decarboxylase and gama-amino-butyrate transporter that affect the GABAergic system, thereby relieve pain-related emotions. These findings provide the references for the mechanism research and clinical application of acupuncture in treatment of pain-related emotions.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1095-1102"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Therapeutic effect of the combination of Tiaodu Lijin acupuncture therapy and Lijin reduction tuina manipulation in treatment of cervical spondylotic radiculopathy].","authors":"Yuan-Hui Feng, Ling-Yu Kong, Ying Sun, Fang Gao","doi":"10.13702/j.1000-0607.20250309","DOIUrl":"10.13702/j.1000-0607.20250309","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the therapeutic effect of the combined measures of Tiaodu Lijin acupuncture therapy (for regulating the governor vessel and tendons) and Lijin reduction tuina manipulation (for regulating tendons and reduction) in treatment of cervical spondylotic radiculopathy (CSR).</p><p><strong>Methods: </strong>From August 2021 to December 2023, 160 CSR patients were selected and randomly divided into a control group (80 cases) and a trial group (80 cases) using a random number table method. In the control group, the conventional acupuncture and Lijin reduction tuina manipulation were operated, and in the trial group, Tiaodu Lijin acupuncture therapy and Lijin reduction tuina manipulation were combined. The treatment was delivered once daily in each group, 5 interventions a week and lasted for 4 weeks. Before and after treatment, the score of clinical assessment scale of cervical spondylosis (CASCS), cervical dysfunction index (NDI) and the score of visual analog scale (VAS) were compared in the two groups. Using ELISA, the contents of interleukin (IL)-6, leukotriene D4 (LTD4), tumor necrosis factor (TNF)-α and C-reactive protein (CRP) in the serum were detected. Transcranial color Doppler ultrasound was used to detect the mean peak velocity (VM), resistance index (RI) and pulsation index (PI) of vertebral-basilar artery at systole. The effective rate was calculated in the two groups.</p><p><strong>Results: </strong>Every indicator after treatment was improved in comparison with that before treatment in each group (<i>P</i><0.05). After 4-week treatment, compared with the control group, NDI and VAS scores were lower (<i>P</i><0.05), and the serum contents of IL-6, LTD4, TNF-α and CRP were reduced (<i>P</i><0.05), RI and PI of vertebral-basilar artery were lower (<i>P</i><0.05), and CASCS score and VM were higher (<i>P</i><0.05) in the trial group. The overall effective rate in the trial group (95.00%, 76/80) was higher (<i>P</i><0.05) than that (81.25%, 65/80) in the control group.</p><p><strong>Conclusions: </strong>The combined measures of Tiaodu Lijin acupuncture therapy and Lijin reduction tuina manipulation obtain the satisfactory therapeutic effect on CSR, which alleviates pain, reduces inflammatory indicators and improves vertebral basilar arterial blood flow and cervical spine function.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1088-1094"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Electroacupuncture of specific acupoints of Shaoyang Meridian relieves progression of chronic migrain by down-regulating expression of P2Y12 receptor in rats].","authors":"Yi Qiu, Yu-Han Huang, Yu-Hui Song, Yu-Han He, Wen-Hua Han, Ke He, Lei Fu, Wei-Ai Liu","doi":"10.13702/j.1000-0607.20240573","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240573","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of electroacupuncture (EA) at specific points of the Shaoyang Meridian on mechanical pain threshold, and expressions of P2Y12 receptor (P2RY12), ionic calcium binding molecule 1 (Iba-1), calcitonin gene-related peptide (CGRP) and c-fos in rats with chronic migraine (CM) , so as to explore its mechanisms underlying prevention and treatment of CM.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Forty-eight SD rats (half male and half female) were randomly divided into 6 groups (using a random number table method):control, model, EA, antagonist, EA+agonist, and medication groups, with 8 rats in each group. The CM model was established by repeated intraperitoneal injection of nitroglycerin (10 mg/kg, once every other day, 5 times altogether). EA (10 Hz/50 Hz, a strength being able to induce local slight regular muscular contraction of the fore-limb) was applied to bilateral \"Fengchi\"(GB20), \"Yanglingquan\"(GB34), and \"Waiguan\"(TE5) for 30 min, once a day before modeling and once every other day during modeling, for a total of 6 times. The rats of the antagonist group received intrathecal injection of P2RY12 antagonist MRS2395 (200 μg/rat) , once every other day for a total of 5 times. Rats of the EA+agonist group received intrathecal injection of P2RY12 agonist ADP (4.272 μg/rat) once every other day for a total of 5 times, and those of the medication group received intraperitoneal injection of topiramate (30 mg/kg) once a day for 9 d. Rats of the other groups were bound for 30 min during EA. On the 1&lt;sup&gt;st&lt;/sup&gt;, 3&lt;sup&gt;rd&lt;/sup&gt;, 5&lt;sup&gt;th&lt;/sup&gt;, 7&lt;sup&gt;th&lt;/sup&gt;, and 9&lt;sup&gt;th&lt;/sup&gt; day of modeling, 2 h before the intervention, the mechanical pain thresholds of the periorbital region of the eye and the plantar region of the hindlimb in each group were measured using a von Frey filament. The number of P2RY12 positive cells and the expression of P2RY12 mRNA in the caudal nucleus of the trigeminal spinal tract (TNC) were detected using immunofluorescence staining and real-time fluorescence quantitative PCR, separately, and the expression levels of P2RY12, Iba-1, CGRP, and c-fos proteins in the TNC tissue were detected using Western blot.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In comparison with the control group, the periorbital and plantar pain thresholds were significantly reduced (&lt;i&gt;P&lt;/i&gt;&lt;0.001), while the expression levels of P2RY12, Iba-1, CGRP, and c-fos proteins in the TNC tissue, and the number of P2RY12 positive cells and P2RY12 mRNA expression were significantly increased in the model group (&lt;i&gt;P&lt;/i&gt;&lt;0.001). Compared with the model group, the periorbital and plantar pain thresholds of the EA, antagonist and medication groups were significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.001), while the expression levels of P2RY12, Iba-1, CGRP, and c-fos proteins, number of P2RY12 positive neurons, and P2RY12 mRNA expression were apparently decreased in the EA, antagonist, medication and EA+agonist groups (&lt;i&gt;P&lt;/i&gt;&lt;0.001, &lt;i&gt;P&lt;/i&gt;&lt;0.05).","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1064-1071"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of \"Huayu Tongluo\" moxibustion on expressions of oligodendrocyte-related proteins and myelin ultrastructure in the corpus callosum in rats with vascular dementia].","authors":"Chen-Hui Gao, Qing-Ping Zhang, Lan Mei, Ming Zhang, Wei-Shun Wang, Jin-Jin Zheng, Yin-Qiu Fan, Hai-Ping Shi, Pin Wang","doi":"10.13702/j.1000-0607.20240658","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20240658","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of \"Huayu Tongluo\"(blood stasis-removing and meridian-collateral dredging) moxibustion on oligodendrocyte-related protein expression and myelin ultrastructure in the corpus callosum tissue of rats with vascular dementia (VD), so as to explore its mechanism underlying improvement of VD.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Male SD rats were randomly divided into model group, \"Huayu Tongluo\" moxibustion group and non-acupoint moxibustion group, with 42 rats in each group. According to the treatment time, the rats were further divided into 1 course (7 d) group (&lt;i&gt;n&lt;/i&gt;=12), 2 courses (14 d) group (&lt;i&gt;n&lt;/i&gt;=12) and 4 courses (28 d) group (&lt;i&gt;n&lt;/i&gt;=18), and the other 18 rats were used as the sham operation group. The VD model was established by using modified 2-vessel occlusion (VO) method. The rats in the \"Huayu Tongluo\" moxibustion group were treated with suspended moxibustion of \"Baihui\"(GV20), \"Dazhui\"(GV14) and \"Shenting\"(GV24) for 20 min, once a day for 7 d, 14 d or 28 d, respectively. The rats of the non-acupoint moxibustion group were treated with suspended moxibustion at the three locations 2, 3 and 5 cm away from the tail, and the course of treatment was the same as that of \"Huayu Tongluo\" moxibustion group. Morris water maze test was used to assess the rats' learning-memory ability. A transmission electron microscopy was used to observe the ultrastructure of myelin sheath and the number of myelinated nerve axons, and to calculate the g-ratio (axon diameter/(axon diameter + myelin sheath thickness)) of the myelin sheath thickness. Western blot and immunofluorescence staining were used to separately detect the expressions and fluorescence intensities of oligodendrocyte markers 2', 3'-cyclic nucleotide-3'-phosphodiesterase (CNPase), oligodendrocyte transcription factor 2 (Olig2) and myelin oligodendrocyte glycoprotein (MOG) in the corpus callosum of each group.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the sham operation group, the model group showed prolonged escape latency (&lt;i&gt;P&lt;/i&gt;&lt;0.01) and reduced number of platform quadrant crossings (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the 1, 2 and 4 courses of treatment, significantly decreased number of myelinated nerve axons in the corpus callosum (&lt;i&gt;P&lt;/i&gt;&lt;0.01), increased g-ratio value of axonal myelin (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and decreased relative fluorescence intensities and expression levels of CNPase, Olig2, and MOG proteins in the 1, 2 and 4 courses of intervention (&lt;i&gt;P&lt;/i&gt;&lt;0.01). Compared with the model group, the \"Huayu Tongluo\" moxibustion group showed shortened escape latency (&lt;i&gt;P&lt;/i&gt;&lt;0.01) and increased number of platform quadrant crossing after 2 and 4 courses of intervention (&lt;i&gt;P&lt;/i&gt;&lt;0.01), significantly increased number of myelinated nerve axons (&lt;i&gt;P&lt;/i&gt;&lt;0.01), decreased g-ratio value of axonal myelin, and significantly elevated relative fluorescence intensities and protein expression levels of CNPase, Olig2, and MOG in all the different courses of inte","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 9","pages":"1046-1054"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}